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pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF

Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3.

Catalog number RDB14530
Resource name pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF
Alternative name Fxa_His- and Fxa_FLAG-tagged Human alpha1bata3 tubulin
Clone info. Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3. This clone is driven by the two promoters (PPH and Pp10) in a single vector for expression of two proteins simultaneously.
Comment Unpublished plasmid. Reference sequence: NP_006073 for TUBA1B, NP_006077 for TUBB3.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Gent^r.
Gene/insert name Human TUBA1B cDNA Human TUBB3 cDNA
Depositor|Developer Muto, Etsuko |
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External Database
Human TUBA1B Human TUBB3

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013)を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
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        RDB14530 pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Purification of Recombinant Tubulin (pdf)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14530_A7A5p1-2 check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pPolyhedrin_F
        Sequence file: RDB14530_A7A5a.seq check
        >D03763A1_A7A5_3_pPolyhedrin_F_C06_09_ABI24.ab1
            1 TATTTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT TCCGGATTAT
           61 TCATACCGTC CCACCATCGG GCGCGGATCC CGGTCCGAAG CGCGCGGAAT TCAAAGGCCT
          121 ACGTCGACGA GCTCACTAGT AACTCCTAAA AAACCGCCAC CATGCGCGAG TGCATCTCCA
          181 TCCACGTGGG CCAGGCTGGT GTCCAGATCG GCAACGCTTG CTGGGAGCTG TACTGCCTGG
          241 AGCACGGTAT CCAGCCCGAC GGCCAGATGC CCTCCGACAA GACCATCGGT GGCGGCGACG
          301 ACTCCTTCAA CACCTTCTTC TCCGAAACCG GTGCTGGCAA GCACGTGCCC CGCGCTGTGT
          361 TCGTGGATCT GGAACCCACC GTGATCGACG AAGTGCGTAC CGGCACCTAC CGTCAGCTGT
          421 TCCACCCCGA GCAACTGATC ACCGGCAAGG AGGACGCTGC TAACAACTAC GCTCGTGGTC
          481 ACTACACCAT CGGCAAGGAA ATCATCGACC TGGTCCTGGA CCGTATCCGC AAGCTGGCTG
          541 ACCAGTGCAC CGGCCTGCAG GGATTCCTCG TGTTCCACTC CTTCGGTGGT GGAACCGGTT
          601 CTGGTTTCAC CTCCCTGCTG ATGGAACGTC TGTCTGTGGA CTACGGCAAG AAGTCCAAGT
          661 TGGAGTTCTC CATCTACCCC GCTCCCCAGG TGTCCACCGC TGTGGTCGAG CCCTACAACT
          721 CCATCCTGAC CACCCACACC ACCCTGGAAC ACTCCGACTG CGCTTTCATG GTGGACAACG
          781 AGGCTATCTA CGACATCTGC CGTCGTAACC TGGACATCGA GCGTCCCACC TACACCAACC
          841 TGAACCGTCT GATCTCCCAG ATCGTGTCCT CCATCACCGC TTCCCTGCGT TTCGACGGTG
          901 CTCTGAACGT GGACCTGACC GAGTTCCAGA CCAACCTGGT GCCCTACCCC CGTATCCACT
          961 TCCCCCTGGC TACCTACGCT CCCGTGATCT CCGGCTGAGA GGCTTACCAC GAGCAGCTGT
         1021 CCGTGGCTGA GATCACCAAC GCCCTGCTTC GAGCCCGCTA ACCAGATGGT CAGTGCGACC
         1081 CCCGTCACGG CAAGTACATG GCTGCTGCCT GCTGTACGTT GCCGACGTGA TGCTAGGAAC
        1141 GTGAACGGCT GCTTATTCGC CTTACCATTC CAGGAACCCC AAGC
        //
        Primer: SV40polyA-R
        Sequence file: RDB14530_A7A5b.seq check
        >D03763A1_A7A5_3_SV40polyA_R(+)_E05_05_ABI08.ab1
            1 AGAAAAATGG GAGGTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
           61 TCCTCTAGTA CTTCTCGACA AGCTTTTAGT GGTGATGGTG GTGATGATGG TGACCACCGG
          121 AACCACCACG ACCCTCGATG TATTCCTCGC CTTCCTCTTC GCCCTCGCCC TCGACGGAGT
          181 CGACGCCCAC TTCCTCGTAG TCCTTTTCCA GAGCAGCCAT GTCCTCGCGA GCCTCGGAGA
          241 ACTCGCCCTC TTCCATGCCT TCGCCCACGT ACCAGTGCAC GAAAGCGCGC TTAGCGTACA
          301 TCAGGTCGAA CTTGTGGTCC AGACGAGCCC AAGCCTCAGC GATAGCGGTG GTGTTGGACA
          361 GCATGCACAC AGCACGCTGC ACCTTAGCCA AGTCGCCACC GGGCACCACA GTGGGGGGCT
          421 GGTAGTTGAT TCCGACCTTG AAACCGGTGG GGCACCAGTC CACGAACTGG ATGGAACGCT
          481 TGGTCTTGAT GGTAGCGATA GCAGCGTTCA CGTCCTTAGG CACCACGTCG CCACGGTACA
          541 GCAGGCAGCA AGCCATGTAC TTGCCGTGAC GGGGGTCGCA CTTGACCATC TGGTTAGCGG
          601 GCTCGAAGCA GGCGTTGGTG ATCTCAGCCA CGGACAGCTG CTCGTGGTAA GCCTTCTCAG
          661 CGGAGATCAC GGGAGCGTAG GTAGCCAGGG GGAAGTGGAT ACGGGGGTAG GGCACCAGGT
          721 TGGTCTGGAA CTCGGTCAGG TCCACGTTCA GAGCACCGTC GAAACGCAGG GAAGCGGTGA
          781 TGGAGGACAC GATCTGGGAG ATCAGACGGT TCAGGTTGGT GTAGGTGGGA CGCTCGATGT
          841 CCAGGTTACG ACGGCAGATG TCGTAGATAG CCTCGTTGTC CACCATGAAA GCGCAGTCGG
          901 AGTGTTCCAG GGTGGTGTGG GTGGGTCAGG GATGGAGTTG TAGGGCTCGA CCACAGCGGG
          961 TGGACACCTG GGGGAGCGGG GGTAGATGGA GAACTCCAAA CTTGGACTTC TTTGCCGTAG
         1021 TCCACAGACA GACGTTTCCA TCAGCAGGGG AGGTGAAACC AGAACCGGTT CACACCGGAA
         1081 AGGAGTGGAA CACGAGAATC CCTGCAGGGC CGGATGCACT GATTCAGGCC AGCTGCCGAA
        1141 TTACCGGATT CCAGGACCAG GGATCGAATG GAATTTTCTC
        //
        Primer: pP10_F
        Sequence file: RDB14530_A7A5c.seq check
        >D03763A1_A7A5_3_pP10_F_A06_01_ABI08.ab1
            1 ACTATAGATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT
           61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA
          121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG
          181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG
          241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA
          301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT
          361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG
          421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG
          481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT
          541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG
          601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA
          661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG
          721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG
          781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA
          841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG
          901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGGTTTCG CTCCTCTGAC CGCTCGCGGG
          961 TTCCCAGCAA TACCGCGCTC TGACCGTGCC CTGAGCTGAC CCAGCAGATG TTCGACGCTA
         1021 AGAACATGAT GGCTGCTTGC GACCCTCGCC ACGGTCGCTA CTGACGTGGC TACCGTGTTC
         1081 CGCGGTCGCA TGTCATGAGA AGTGGACGAG CGGATGCTGC TATCCAGTCA GGACTTCTTC
        1141 TTACCTCGTT GGAGGTGATT CCCTTAAACC AAAC
        //
        Primer: HSV_TK_pA_R
        Sequence file: RDB14530_A7A5d.seq check
        >D03763A1_A7A5_3_HSV_TK_pA_R_F06_18_ABI24.ab1
            1 GTCGGGGGGT TCTTTCGGGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC
           61 CCGGTACCGC ATGCTATGCA TCAGCTGCTA GCTTACTTGT CATCGTCATC TTTGTAGTCA
          121 CCACCGGAAC CACCACGACC CTCGATCTTA GGACCCTGAG CCTCGGACTC TTCCTCATCG
          181 TCCTCGTACA TCTCACCCTC TTCTTCAGCG GTAGCGTCCT GGTACTGCTG GTACTCGGAC
          241 ACCAGGTCGT TCATGTTGGA CTCAGCCTCG GTGAACTCCA TCTCGTCCAT ACCCTCACCG
          301 GTGTACCAGT GCAGGAAAGC CTTGCGGCGG AACATAGCGG TGAACTGCTC GGAGATGCGC
          361 TTGAACAGCT CCTGGATAGC GGTGGAGTTA CCGATGAAGG TGGAGGACAT CTTCAGACCG
          421 CGAGGAGGGA TGTCGCACAC AGCCACCTTC ACGTTGTTAG GGATCCACTC CACGAAGTAG
          481 GAGGAGTTCT TGGACTGGAT AGCCAGCATC TGCTCGTCCA CCTCCTTCAT GGACATGCGA
          541 CCGCGGAACA CGGTAGCCAC GGTCAGGTAG CGACCGTGGC GAGGGTCGCA AGCAGCCATC
          601 ATGTTCTTAG CGTCGAACAT CTGCTGGGTC AGCTCAGGCA CGGTCAGAGC GCGGTATTGC
          661 TGGGAACCGC GAGCGGTCAG AGGAGCGAAA CCAGGCATGA AGAAGTGCAG GCGAGGGAAA
          721 GGCACCATGT TCACAGCCAG CTTGCGCAGG TCAGCGTTCA GCTGACCAGG GAAGCGCAGG
          781 GAGGTGGTCA CACCGGACAT GGTAGCGGAG ACCAGGTGGT TCAGGTCACC GTAGGTAGGG
          841 GTAGCCAGCT TCAGGGTGCG GAAGCAGATG TCGTACAGAG CCTCGTTGTC GATGCAGTAG
          901 GTCTCGTCGG TGTTCTCCAC CAGTTGGTGG GATGGACAGG GGTAGCGTTG TAAGGCTCCA
          961 CCACGGTGTC GGACACCCTT AGGGGAAGGC ACGACGGGAG AAAGGTATTC ATGATGCGGT
         1021 CAGGTTACTC CTCGCGGCAC CTTTGGAGAA TCAGCAGGGT TACCCATACC AGATCCGGGT
         1081 ACCACCCACC CCAAGGGAGT GAGTCAGCTT TGAAACCCTT GCAGCCAGTT CGCAGTTCTC
        1141 GCACCTCCTT TGCGCCACCA ACCGATCCAG GCAACGAGAA GGTCTCGAAC CGAGA
        //
        Primer: f1ori_F
        Sequence file: RDB14530_A7A5e.seq check
        >D03763A1_A7A5_3_f1ori_F_E03_15_ABI24.ab1
            1 CAGGGGCTAG GCGCTGGCAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG
           61 CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCAAAT AAGCGTTGAT
          121 ATTCAGTCAA TTACAAACAT TAATAACGAA GAGATGACAG AAAAATTTTC ATTCTGTGAC
          181 AGAGAAAAAG TAGCCGAAGA TGACGGTTTG TCACATGGAG TTGGCAGGAT GTTTGATTAA
          241 AAACATAACA GGAAGAAAAA TGCCCCGCTG TGGGCGGACA AAATAGTTGG GAACTGGGAG
          301 GGGTGGAAAT GGAGTTTTTA AGGATTATTT AGGGAAGAGT GACAAAATAG ATGGGAACTG
          361 GGTGTAGCGT CGTAAGCTAA TACGAAAATT AAAAATGACA AAATAGTTTG GAACTAGATT
          421 TCACTTATCT GGTTCGGATC TCCTAGGCTC AAGCAGTGAT CAGATCCAGA CATGATAAGA
          481 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
          541 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
          601 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
          661 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGT ACTTCTCGAC
          721 AAGCTTTTAG TGGTGATGGT GGTGATGATG GTGACCACCG GAACCACCAC GACCCTCGAT
          781 GTATTCCTCG CCTTCCTCTT CGCCCTCGCC CTCGACGGAG TCGACGCCCA CTTCCTCGTA
          841 GTCCTTTTCC AGAGCAGCCA TGTCCTCGCG AGCCTCGGAG AACTCGCCCT CTTCCATGCC
          901 TTCGCCCACG TACCAGTGCA CGAAAGCGCG CTTAGCGTAC ATCAGGTCGA ACTTGTGGGT
          961 CCAGACGAGC CCAAAGCCCT CAGCGATAGC GGTGATGTGG GACAGCATGC ACAACAGCAA
         1021 CGGCTGGCAC CTTTAGCCCA AGGTCGCCAA CCGGGCAACA CAGTTGAGGA GCTGGTAGTT
        1081 GAATTCCGAA TCTTGCAACT CGAATGCATA
        //
        Primer: Pcpro_F
        Sequence file: RDB14530_A7A5f.seq check
        >D03763A1_A7A5_3_Pcpro_F_G06_21_ABI24.ab1
            1 AAGGCGTTTG CGCTCACGCA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC
           61 GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG TACAGTCTAT GCCTCGGGCA
          121 TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT
          181 GTTACGCAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAGGTGG
          241 CTCAAGTATG GGCATCATTC GCACATGTAG GCTCGGCCCT GACCAAGTCA AATCCATGCG
          301 GGCTGCTCTT GATCTTTTCG GTCGTGAGTT CGGAGACGTA GCCACCTACT CCCAACATCA
          361 GCCGGACTCC GATTACCTCG GGAACTTGCT CCGTAGTAAG ACATTCATCG CGCTTGCTGC
          421 CTTCGACCAA GAAGCGGTTG TTGGCGCTCT CGCGGCTTAC GTTCTGCCCA AGTTTGAGCA
          481 GCCGCGTAGT GAGATCTATA TCTATGATCT CGCAGTCTCC GGCGAGCACC GGAGGCAGGG
          541 CATTGCCACC GCGCTCATCA ATCTCCTCAA GCATGAGGCC AACGCGCTTG GTGCTTATGT
          601 GATCTACGTG CAAGCAGATT ACGGTGACGA TCCCGCAGTG GCTCTCTATA CAAAGTTGGG
          661 CATACGGGAA GAAGTGATGC ACTTTGATAT CGACCCAAGT ACCGCCACCT AACAATTCGT
          721 TCAAGCCGAG ATCGGCTTCC CGGCCGCGGA GTTGTTCGGT AAATTGTCAC AACGCCGCGA
          781 ATATAGTCTT TACCATGCCC TTGGCCACGC CCCTCTTTAA TACGACGGGC AATTTGCACT
          841 TCAGAAAATG AAGAGTTTGC TTTAGCCATA ACAAAAGTCC AGTATGCTTT TTCACAGCAT
          901 AACTGGACTG ATTTCAGTTT ACAACTATTC TGTCTAGTTT AAGACTTTAT TGTCATAGTT
          961 TAGATCTATT TTGTTCAGTT TAAGACTTTA TTTGTCCGCC CACACCCGCT TTACGCAGGG
         1021 CATCCATTTA TTACTCAACC GTAAACCGAT TTTGCCAGGC TACGCGGCTG GTCTATGCGT
         1081 GGTGAAATAC GCACAGATGC GTAAGGGAGA AATACGCATC AGCGCCTCTT CGCTTCGTCG
        1141 CTCACTTGAA CTTCCGCCT
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        reference Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl