Resource data sheet
Prev.

pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF

Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3.

Catalog number RDB14530
Resource name pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF
Alternative name Fxa_His- and Fxa_FLAG-tagged Human alpha1bata3 tubulin
Clone info. Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3. This clone is driven by the two promoters (PPH and Pp10) in a single vector for expression of two proteins simultaneously.
Unpublished plasmid. Reference sequence: NP_006073 for TUBA1B, NP_006077 for TUBB3.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Gent^r.
Gene/insert name Human TUBA1B cDNA Human TUBB3 cDNA
Depositor Muto, Etsuko |
 
Other clones in our bank human TUBA1B (NCBI Gene 10376) | human TUBB3 (NCBI Gene 10381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14530 pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Purification of Recombinant Tubulin (pdf)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14530_A7A5p1-2

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F RDB14530_A7A5a.seq
>D03763A1_A7A5_3_pPolyhedrin_F_C06_09_ABI24.ab1
    1 TATTTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT TCCGGATTAT
   61 TCATACCGTC CCACCATCGG GCGCGGATCC CGGTCCGAAG CGCGCGGAAT TCAAAGGCCT
  121 ACGTCGACGA GCTCACTAGT AACTCCTAAA AAACCGCCAC CATGCGCGAG TGCATCTCCA
  181 TCCACGTGGG CCAGGCTGGT GTCCAGATCG GCAACGCTTG CTGGGAGCTG TACTGCCTGG
  241 AGCACGGTAT CCAGCCCGAC GGCCAGATGC CCTCCGACAA GACCATCGGT GGCGGCGACG
  301 ACTCCTTCAA CACCTTCTTC TCCGAAACCG GTGCTGGCAA GCACGTGCCC CGCGCTGTGT
  361 TCGTGGATCT GGAACCCACC GTGATCGACG AAGTGCGTAC CGGCACCTAC CGTCAGCTGT
  421 TCCACCCCGA GCAACTGATC ACCGGCAAGG AGGACGCTGC TAACAACTAC GCTCGTGGTC
  481 ACTACACCAT CGGCAAGGAA ATCATCGACC TGGTCCTGGA CCGTATCCGC AAGCTGGCTG
  541 ACCAGTGCAC CGGCCTGCAG GGATTCCTCG TGTTCCACTC CTTCGGTGGT GGAACCGGTT
  601 CTGGTTTCAC CTCCCTGCTG ATGGAACGTC TGTCTGTGGA CTACGGCAAG AAGTCCAAGT
  661 TGGAGTTCTC CATCTACCCC GCTCCCCAGG TGTCCACCGC TGTGGTCGAG CCCTACAACT
  721 CCATCCTGAC CACCCACACC ACCCTGGAAC ACTCCGACTG CGCTTTCATG GTGGACAACG
  781 AGGCTATCTA CGACATCTGC CGTCGTAACC TGGACATCGA GCGTCCCACC TACACCAACC
  841 TGAACCGTCT GATCTCCCAG ATCGTGTCCT CCATCACCGC TTCCCTGCGT TTCGACGGTG
  901 CTCTGAACGT GGACCTGACC GAGTTCCAGA CCAACCTGGT GCCCTACCCC CGTATCCACT
  961 TCCCCCTGGC TACCTACGCT CCCGTGATCT CCGGCTGAGA GGCTTACCAC GAGCAGCTGT
 1021 CCGTGGCTGA GATCACCAAC GCCCTGCTTC GAGCCCGCTA ACCAGATGGT CAGTGCGACC
 1081 CCCGTCACGG CAAGTACATG GCTGCTGCCT GCTGTACGTT GCCGACGTGA TGCTAGGAAC
1141 GTGAACGGCT GCTTATTCGC CTTACCATTC CAGGAACCCC AAGC
//
Primer: SV40polyA-R RDB14530_A7A5b.seq
>D03763A1_A7A5_3_SV40polyA_R(+)_E05_05_ABI08.ab1
    1 AGAAAAATGG GAGGTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
   61 TCCTCTAGTA CTTCTCGACA AGCTTTTAGT GGTGATGGTG GTGATGATGG TGACCACCGG
  121 AACCACCACG ACCCTCGATG TATTCCTCGC CTTCCTCTTC GCCCTCGCCC TCGACGGAGT
  181 CGACGCCCAC TTCCTCGTAG TCCTTTTCCA GAGCAGCCAT GTCCTCGCGA GCCTCGGAGA
  241 ACTCGCCCTC TTCCATGCCT TCGCCCACGT ACCAGTGCAC GAAAGCGCGC TTAGCGTACA
  301 TCAGGTCGAA CTTGTGGTCC AGACGAGCCC AAGCCTCAGC GATAGCGGTG GTGTTGGACA
  361 GCATGCACAC AGCACGCTGC ACCTTAGCCA AGTCGCCACC GGGCACCACA GTGGGGGGCT
  421 GGTAGTTGAT TCCGACCTTG AAACCGGTGG GGCACCAGTC CACGAACTGG ATGGAACGCT
  481 TGGTCTTGAT GGTAGCGATA GCAGCGTTCA CGTCCTTAGG CACCACGTCG CCACGGTACA
  541 GCAGGCAGCA AGCCATGTAC TTGCCGTGAC GGGGGTCGCA CTTGACCATC TGGTTAGCGG
  601 GCTCGAAGCA GGCGTTGGTG ATCTCAGCCA CGGACAGCTG CTCGTGGTAA GCCTTCTCAG
  661 CGGAGATCAC GGGAGCGTAG GTAGCCAGGG GGAAGTGGAT ACGGGGGTAG GGCACCAGGT
  721 TGGTCTGGAA CTCGGTCAGG TCCACGTTCA GAGCACCGTC GAAACGCAGG GAAGCGGTGA
  781 TGGAGGACAC GATCTGGGAG ATCAGACGGT TCAGGTTGGT GTAGGTGGGA CGCTCGATGT
  841 CCAGGTTACG ACGGCAGATG TCGTAGATAG CCTCGTTGTC CACCATGAAA GCGCAGTCGG
  901 AGTGTTCCAG GGTGGTGTGG GTGGGTCAGG GATGGAGTTG TAGGGCTCGA CCACAGCGGG
  961 TGGACACCTG GGGGAGCGGG GGTAGATGGA GAACTCCAAA CTTGGACTTC TTTGCCGTAG
 1021 TCCACAGACA GACGTTTCCA TCAGCAGGGG AGGTGAAACC AGAACCGGTT CACACCGGAA
 1081 AGGAGTGGAA CACGAGAATC CCTGCAGGGC CGGATGCACT GATTCAGGCC AGCTGCCGAA
1141 TTACCGGATT CCAGGACCAG GGATCGAATG GAATTTTCTC
//
Primer: pP10_F RDB14530_A7A5c.seq
>D03763A1_A7A5_3_pP10_F_A06_01_ABI08.ab1
    1 ACTATAGATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT
   61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA
  121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG
  181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG
  241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA
  301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT
  361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG
  421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG
  481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT
  541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG
  601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA
  661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG
  721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG
  781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA
  841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG
  901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGGTTTCG CTCCTCTGAC CGCTCGCGGG
  961 TTCCCAGCAA TACCGCGCTC TGACCGTGCC CTGAGCTGAC CCAGCAGATG TTCGACGCTA
 1021 AGAACATGAT GGCTGCTTGC GACCCTCGCC ACGGTCGCTA CTGACGTGGC TACCGTGTTC
 1081 CGCGGTCGCA TGTCATGAGA AGTGGACGAG CGGATGCTGC TATCCAGTCA GGACTTCTTC
1141 TTACCTCGTT GGAGGTGATT CCCTTAAACC AAAC
//
Primer: HSV_TK_pA_R RDB14530_A7A5d.seq
>D03763A1_A7A5_3_HSV_TK_pA_R_F06_18_ABI24.ab1
    1 GTCGGGGGGT TCTTTCGGGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC
   61 CCGGTACCGC ATGCTATGCA TCAGCTGCTA GCTTACTTGT CATCGTCATC TTTGTAGTCA
  121 CCACCGGAAC CACCACGACC CTCGATCTTA GGACCCTGAG CCTCGGACTC TTCCTCATCG
  181 TCCTCGTACA TCTCACCCTC TTCTTCAGCG GTAGCGTCCT GGTACTGCTG GTACTCGGAC
  241 ACCAGGTCGT TCATGTTGGA CTCAGCCTCG GTGAACTCCA TCTCGTCCAT ACCCTCACCG
  301 GTGTACCAGT GCAGGAAAGC CTTGCGGCGG AACATAGCGG TGAACTGCTC GGAGATGCGC
  361 TTGAACAGCT CCTGGATAGC GGTGGAGTTA CCGATGAAGG TGGAGGACAT CTTCAGACCG
  421 CGAGGAGGGA TGTCGCACAC AGCCACCTTC ACGTTGTTAG GGATCCACTC CACGAAGTAG
  481 GAGGAGTTCT TGGACTGGAT AGCCAGCATC TGCTCGTCCA CCTCCTTCAT GGACATGCGA
  541 CCGCGGAACA CGGTAGCCAC GGTCAGGTAG CGACCGTGGC GAGGGTCGCA AGCAGCCATC
  601 ATGTTCTTAG CGTCGAACAT CTGCTGGGTC AGCTCAGGCA CGGTCAGAGC GCGGTATTGC
  661 TGGGAACCGC GAGCGGTCAG AGGAGCGAAA CCAGGCATGA AGAAGTGCAG GCGAGGGAAA
  721 GGCACCATGT TCACAGCCAG CTTGCGCAGG TCAGCGTTCA GCTGACCAGG GAAGCGCAGG
  781 GAGGTGGTCA CACCGGACAT GGTAGCGGAG ACCAGGTGGT TCAGGTCACC GTAGGTAGGG
  841 GTAGCCAGCT TCAGGGTGCG GAAGCAGATG TCGTACAGAG CCTCGTTGTC GATGCAGTAG
  901 GTCTCGTCGG TGTTCTCCAC CAGTTGGTGG GATGGACAGG GGTAGCGTTG TAAGGCTCCA
  961 CCACGGTGTC GGACACCCTT AGGGGAAGGC ACGACGGGAG AAAGGTATTC ATGATGCGGT
 1021 CAGGTTACTC CTCGCGGCAC CTTTGGAGAA TCAGCAGGGT TACCCATACC AGATCCGGGT
 1081 ACCACCCACC CCAAGGGAGT GAGTCAGCTT TGAAACCCTT GCAGCCAGTT CGCAGTTCTC
1141 GCACCTCCTT TGCGCCACCA ACCGATCCAG GCAACGAGAA GGTCTCGAAC CGAGA
//
Primer: f1ori_F RDB14530_A7A5e.seq
>D03763A1_A7A5_3_f1ori_F_E03_15_ABI24.ab1
    1 CAGGGGCTAG GCGCTGGCAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG
   61 CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCAAAT AAGCGTTGAT
  121 ATTCAGTCAA TTACAAACAT TAATAACGAA GAGATGACAG AAAAATTTTC ATTCTGTGAC
  181 AGAGAAAAAG TAGCCGAAGA TGACGGTTTG TCACATGGAG TTGGCAGGAT GTTTGATTAA
  241 AAACATAACA GGAAGAAAAA TGCCCCGCTG TGGGCGGACA AAATAGTTGG GAACTGGGAG
  301 GGGTGGAAAT GGAGTTTTTA AGGATTATTT AGGGAAGAGT GACAAAATAG ATGGGAACTG
  361 GGTGTAGCGT CGTAAGCTAA TACGAAAATT AAAAATGACA AAATAGTTTG GAACTAGATT
  421 TCACTTATCT GGTTCGGATC TCCTAGGCTC AAGCAGTGAT CAGATCCAGA CATGATAAGA
  481 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
  541 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
  601 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
  661 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGT ACTTCTCGAC
  721 AAGCTTTTAG TGGTGATGGT GGTGATGATG GTGACCACCG GAACCACCAC GACCCTCGAT
  781 GTATTCCTCG CCTTCCTCTT CGCCCTCGCC CTCGACGGAG TCGACGCCCA CTTCCTCGTA
  841 GTCCTTTTCC AGAGCAGCCA TGTCCTCGCG AGCCTCGGAG AACTCGCCCT CTTCCATGCC
  901 TTCGCCCACG TACCAGTGCA CGAAAGCGCG CTTAGCGTAC ATCAGGTCGA ACTTGTGGGT
  961 CCAGACGAGC CCAAAGCCCT CAGCGATAGC GGTGATGTGG GACAGCATGC ACAACAGCAA
 1021 CGGCTGGCAC CTTTAGCCCA AGGTCGCCAA CCGGGCAACA CAGTTGAGGA GCTGGTAGTT
1081 GAATTCCGAA TCTTGCAACT CGAATGCATA
//
Primer: Pcpro_F RDB14530_A7A5f.seq
>D03763A1_A7A5_3_Pcpro_F_G06_21_ABI24.ab1
    1 AAGGCGTTTG CGCTCACGCA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC
   61 GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG TACAGTCTAT GCCTCGGGCA
  121 TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT
  181 GTTACGCAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAGGTGG
  241 CTCAAGTATG GGCATCATTC GCACATGTAG GCTCGGCCCT GACCAAGTCA AATCCATGCG
  301 GGCTGCTCTT GATCTTTTCG GTCGTGAGTT CGGAGACGTA GCCACCTACT CCCAACATCA
  361 GCCGGACTCC GATTACCTCG GGAACTTGCT CCGTAGTAAG ACATTCATCG CGCTTGCTGC
  421 CTTCGACCAA GAAGCGGTTG TTGGCGCTCT CGCGGCTTAC GTTCTGCCCA AGTTTGAGCA
  481 GCCGCGTAGT GAGATCTATA TCTATGATCT CGCAGTCTCC GGCGAGCACC GGAGGCAGGG
  541 CATTGCCACC GCGCTCATCA ATCTCCTCAA GCATGAGGCC AACGCGCTTG GTGCTTATGT
  601 GATCTACGTG CAAGCAGATT ACGGTGACGA TCCCGCAGTG GCTCTCTATA CAAAGTTGGG
  661 CATACGGGAA GAAGTGATGC ACTTTGATAT CGACCCAAGT ACCGCCACCT AACAATTCGT
  721 TCAAGCCGAG ATCGGCTTCC CGGCCGCGGA GTTGTTCGGT AAATTGTCAC AACGCCGCGA
  781 ATATAGTCTT TACCATGCCC TTGGCCACGC CCCTCTTTAA TACGACGGGC AATTTGCACT
  841 TCAGAAAATG AAGAGTTTGC TTTAGCCATA ACAAAAGTCC AGTATGCTTT TTCACAGCAT
  901 AACTGGACTG ATTTCAGTTT ACAACTATTC TGTCTAGTTT AAGACTTTAT TGTCATAGTT
  961 TAGATCTATT TTGTTCAGTT TAAGACTTTA TTTGTCCGCC CACACCCGCT TTACGCAGGG
 1021 CATCCATTTA TTACTCAACC GTAAACCGAT TTTGCCAGGC TACGCGGCTG GTCTATGCGT
 1081 GGTGAAATAC GCACAGATGC GTAAGGGAGA AATACGCATC AGCGCCTCTT CGCTTCGTCG
1141 CTCACTTGAA CTTCCGCCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

reference Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl