Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF

Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013)を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14530_A7A5p1-2

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F Sequence file: RDB14530_A7A5a.seq
>D03763A1_A7A5_3_pPolyhedrin_F_C06_09_ABI24.ab1     1 TATTTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT TCCGGATTAT    61 TCATACCGTC CCACCATCGG GCGCGGATCC CGGTCCGAAG CGCGCGGAAT TCAAAGGCCT   121 ACGTCGACGA GCTCACTAGT AACTCCTAAA AAACCGCCAC CATGCGCGAG TGCATCTCCA   181 TCCACGTGGG CCAGGCTGGT GTCCAGATCG GCAACGCTTG CTGGGAGCTG TACTGCCTGG   241 AGCACGGTAT CCAGCCCGAC GGCCAGATGC CCTCCGACAA GACCATCGGT GGCGGCGACG   301 ACTCCTTCAA CACCTTCTTC TCCGAAACCG GTGCTGGCAA GCACGTGCCC CGCGCTGTGT   361 TCGTGGATCT GGAACCCACC GTGATCGACG AAGTGCGTAC CGGCACCTAC CGTCAGCTGT   421 TCCACCCCGA GCAACTGATC ACCGGCAAGG AGGACGCTGC TAACAACTAC GCTCGTGGTC   481 ACTACACCAT CGGCAAGGAA ATCATCGACC TGGTCCTGGA CCGTATCCGC AAGCTGGCTG   541 ACCAGTGCAC CGGCCTGCAG GGATTCCTCG TGTTCCACTC CTTCGGTGGT GGAACCGGTT   601 CTGGTTTCAC CTCCCTGCTG ATGGAACGTC TGTCTGTGGA CTACGGCAAG AAGTCCAAGT   661 TGGAGTTCTC CATCTACCCC GCTCCCCAGG TGTCCACCGC TGTGGTCGAG CCCTACAACT   721 CCATCCTGAC CACCCACACC ACCCTGGAAC ACTCCGACTG CGCTTTCATG GTGGACAACG   781 AGGCTATCTA CGACATCTGC CGTCGTAACC TGGACATCGA GCGTCCCACC TACACCAACC   841 TGAACCGTCT GATCTCCCAG ATCGTGTCCT CCATCACCGC TTCCCTGCGT TTCGACGGTG   901 CTCTGAACGT GGACCTGACC GAGTTCCAGA CCAACCTGGT GCCCTACCCC CGTATCCACT   961 TCCCCCTGGC TACCTACGCT CCCGTGATCT CCGGCTGAGA GGCTTACCAC GAGCAGCTGT  1021 CCGTGGCTGA GATCACCAAC GCCCTGCTTC GAGCCCGCTA ACCAGATGGT CAGTGCGACC  1081 CCCGTCACGG CAAGTACATG GCTGCTGCCT GCTGTACGTT GCCGACGTGA TGCTAGGAAC 1141 GTGAACGGCT GCTTATTCGC CTTACCATTC CAGGAACCCC AAGC //
Primer: SV40polyA-R Sequence file: RDB14530_A7A5b.seq
>D03763A1_A7A5_3_SV40polyA_R(+)_E05_05_ABI08.ab1     1 AGAAAAATGG GAGGTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA    61 TCCTCTAGTA CTTCTCGACA AGCTTTTAGT GGTGATGGTG GTGATGATGG TGACCACCGG   121 AACCACCACG ACCCTCGATG TATTCCTCGC CTTCCTCTTC GCCCTCGCCC TCGACGGAGT   181 CGACGCCCAC TTCCTCGTAG TCCTTTTCCA GAGCAGCCAT GTCCTCGCGA GCCTCGGAGA   241 ACTCGCCCTC TTCCATGCCT TCGCCCACGT ACCAGTGCAC GAAAGCGCGC TTAGCGTACA   301 TCAGGTCGAA CTTGTGGTCC AGACGAGCCC AAGCCTCAGC GATAGCGGTG GTGTTGGACA   361 GCATGCACAC AGCACGCTGC ACCTTAGCCA AGTCGCCACC GGGCACCACA GTGGGGGGCT   421 GGTAGTTGAT TCCGACCTTG AAACCGGTGG GGCACCAGTC CACGAACTGG ATGGAACGCT   481 TGGTCTTGAT GGTAGCGATA GCAGCGTTCA CGTCCTTAGG CACCACGTCG CCACGGTACA   541 GCAGGCAGCA AGCCATGTAC TTGCCGTGAC GGGGGTCGCA CTTGACCATC TGGTTAGCGG   601 GCTCGAAGCA GGCGTTGGTG ATCTCAGCCA CGGACAGCTG CTCGTGGTAA GCCTTCTCAG   661 CGGAGATCAC GGGAGCGTAG GTAGCCAGGG GGAAGTGGAT ACGGGGGTAG GGCACCAGGT   721 TGGTCTGGAA CTCGGTCAGG TCCACGTTCA GAGCACCGTC GAAACGCAGG GAAGCGGTGA   781 TGGAGGACAC GATCTGGGAG ATCAGACGGT TCAGGTTGGT GTAGGTGGGA CGCTCGATGT   841 CCAGGTTACG ACGGCAGATG TCGTAGATAG CCTCGTTGTC CACCATGAAA GCGCAGTCGG   901 AGTGTTCCAG GGTGGTGTGG GTGGGTCAGG GATGGAGTTG TAGGGCTCGA CCACAGCGGG   961 TGGACACCTG GGGGAGCGGG GGTAGATGGA GAACTCCAAA CTTGGACTTC TTTGCCGTAG  1021 TCCACAGACA GACGTTTCCA TCAGCAGGGG AGGTGAAACC AGAACCGGTT CACACCGGAA  1081 AGGAGTGGAA CACGAGAATC CCTGCAGGGC CGGATGCACT GATTCAGGCC AGCTGCCGAA 1141 TTACCGGATT CCAGGACCAG GGATCGAATG GAATTTTCTC //
Primer: pP10_F Sequence file: RDB14530_A7A5c.seq
>D03763A1_A7A5_3_pP10_F_A06_01_ABI08.ab1     1 ACTATAGATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT    61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA   121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG   181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG   241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA   301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT   361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG   421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG   481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT   541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG   601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA   661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG   721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG   781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA   841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG   901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGGTTTCG CTCCTCTGAC CGCTCGCGGG   961 TTCCCAGCAA TACCGCGCTC TGACCGTGCC CTGAGCTGAC CCAGCAGATG TTCGACGCTA  1021 AGAACATGAT GGCTGCTTGC GACCCTCGCC ACGGTCGCTA CTGACGTGGC TACCGTGTTC  1081 CGCGGTCGCA TGTCATGAGA AGTGGACGAG CGGATGCTGC TATCCAGTCA GGACTTCTTC 1141 TTACCTCGTT GGAGGTGATT CCCTTAAACC AAAC //
Primer: HSV_TK_pA_R Sequence file: RDB14530_A7A5d.seq
>D03763A1_A7A5_3_HSV_TK_pA_R_F06_18_ABI24.ab1     1 GTCGGGGGGT TCTTTCGGGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC    61 CCGGTACCGC ATGCTATGCA TCAGCTGCTA GCTTACTTGT CATCGTCATC TTTGTAGTCA   121 CCACCGGAAC CACCACGACC CTCGATCTTA GGACCCTGAG CCTCGGACTC TTCCTCATCG   181 TCCTCGTACA TCTCACCCTC TTCTTCAGCG GTAGCGTCCT GGTACTGCTG GTACTCGGAC   241 ACCAGGTCGT TCATGTTGGA CTCAGCCTCG GTGAACTCCA TCTCGTCCAT ACCCTCACCG   301 GTGTACCAGT GCAGGAAAGC CTTGCGGCGG AACATAGCGG TGAACTGCTC GGAGATGCGC   361 TTGAACAGCT CCTGGATAGC GGTGGAGTTA CCGATGAAGG TGGAGGACAT CTTCAGACCG   421 CGAGGAGGGA TGTCGCACAC AGCCACCTTC ACGTTGTTAG GGATCCACTC CACGAAGTAG   481 GAGGAGTTCT TGGACTGGAT AGCCAGCATC TGCTCGTCCA CCTCCTTCAT GGACATGCGA   541 CCGCGGAACA CGGTAGCCAC GGTCAGGTAG CGACCGTGGC GAGGGTCGCA AGCAGCCATC   601 ATGTTCTTAG CGTCGAACAT CTGCTGGGTC AGCTCAGGCA CGGTCAGAGC GCGGTATTGC   661 TGGGAACCGC GAGCGGTCAG AGGAGCGAAA CCAGGCATGA AGAAGTGCAG GCGAGGGAAA   721 GGCACCATGT TCACAGCCAG CTTGCGCAGG TCAGCGTTCA GCTGACCAGG GAAGCGCAGG   781 GAGGTGGTCA CACCGGACAT GGTAGCGGAG ACCAGGTGGT TCAGGTCACC GTAGGTAGGG   841 GTAGCCAGCT TCAGGGTGCG GAAGCAGATG TCGTACAGAG CCTCGTTGTC GATGCAGTAG   901 GTCTCGTCGG TGTTCTCCAC CAGTTGGTGG GATGGACAGG GGTAGCGTTG TAAGGCTCCA   961 CCACGGTGTC GGACACCCTT AGGGGAAGGC ACGACGGGAG AAAGGTATTC ATGATGCGGT  1021 CAGGTTACTC CTCGCGGCAC CTTTGGAGAA TCAGCAGGGT TACCCATACC AGATCCGGGT  1081 ACCACCCACC CCAAGGGAGT GAGTCAGCTT TGAAACCCTT GCAGCCAGTT CGCAGTTCTC 1141 GCACCTCCTT TGCGCCACCA ACCGATCCAG GCAACGAGAA GGTCTCGAAC CGAGA //
Primer: f1ori_F Sequence file: RDB14530_A7A5e.seq
>D03763A1_A7A5_3_f1ori_F_E03_15_ABI24.ab1     1 CAGGGGCTAG GCGCTGGCAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG    61 CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCAAAT AAGCGTTGAT   121 ATTCAGTCAA TTACAAACAT TAATAACGAA GAGATGACAG AAAAATTTTC ATTCTGTGAC   181 AGAGAAAAAG TAGCCGAAGA TGACGGTTTG TCACATGGAG TTGGCAGGAT GTTTGATTAA   241 AAACATAACA GGAAGAAAAA TGCCCCGCTG TGGGCGGACA AAATAGTTGG GAACTGGGAG   301 GGGTGGAAAT GGAGTTTTTA AGGATTATTT AGGGAAGAGT GACAAAATAG ATGGGAACTG   361 GGTGTAGCGT CGTAAGCTAA TACGAAAATT AAAAATGACA AAATAGTTTG GAACTAGATT   421 TCACTTATCT GGTTCGGATC TCCTAGGCTC AAGCAGTGAT CAGATCCAGA CATGATAAGA   481 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT   541 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC   601 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA   661 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGT ACTTCTCGAC   721 AAGCTTTTAG TGGTGATGGT GGTGATGATG GTGACCACCG GAACCACCAC GACCCTCGAT   781 GTATTCCTCG CCTTCCTCTT CGCCCTCGCC CTCGACGGAG TCGACGCCCA CTTCCTCGTA   841 GTCCTTTTCC AGAGCAGCCA TGTCCTCGCG AGCCTCGGAG AACTCGCCCT CTTCCATGCC   901 TTCGCCCACG TACCAGTGCA CGAAAGCGCG CTTAGCGTAC ATCAGGTCGA ACTTGTGGGT   961 CCAGACGAGC CCAAAGCCCT CAGCGATAGC GGTGATGTGG GACAGCATGC ACAACAGCAA  1021 CGGCTGGCAC CTTTAGCCCA AGGTCGCCAA CCGGGCAACA CAGTTGAGGA GCTGGTAGTT 1081 GAATTCCGAA TCTTGCAACT CGAATGCATA //
Primer: Pcpro_F Sequence file: RDB14530_A7A5f.seq
>D03763A1_A7A5_3_Pcpro_F_G06_21_ABI24.ab1     1 AAGGCGTTTG CGCTCACGCA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC    61 GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG TACAGTCTAT GCCTCGGGCA   121 TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT   181 GTTACGCAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAGGTGG   241 CTCAAGTATG GGCATCATTC GCACATGTAG GCTCGGCCCT GACCAAGTCA AATCCATGCG   301 GGCTGCTCTT GATCTTTTCG GTCGTGAGTT CGGAGACGTA GCCACCTACT CCCAACATCA   361 GCCGGACTCC GATTACCTCG GGAACTTGCT CCGTAGTAAG ACATTCATCG CGCTTGCTGC   421 CTTCGACCAA GAAGCGGTTG TTGGCGCTCT CGCGGCTTAC GTTCTGCCCA AGTTTGAGCA   481 GCCGCGTAGT GAGATCTATA TCTATGATCT CGCAGTCTCC GGCGAGCACC GGAGGCAGGG   541 CATTGCCACC GCGCTCATCA ATCTCCTCAA GCATGAGGCC AACGCGCTTG GTGCTTATGT   601 GATCTACGTG CAAGCAGATT ACGGTGACGA TCCCGCAGTG GCTCTCTATA CAAAGTTGGG   661 CATACGGGAA GAAGTGATGC ACTTTGATAT CGACCCAAGT ACCGCCACCT AACAATTCGT   721 TCAAGCCGAG ATCGGCTTCC CGGCCGCGGA GTTGTTCGGT AAATTGTCAC AACGCCGCGA   781 ATATAGTCTT TACCATGCCC TTGGCCACGC CCCTCTTTAA TACGACGGGC AATTTGCACT   841 TCAGAAAATG AAGAGTTTGC TTTAGCCATA ACAAAAGTCC AGTATGCTTT TTCACAGCAT   901 AACTGGACTG ATTTCAGTTT ACAACTATTC TGTCTAGTTT AAGACTTTAT TGTCATAGTT   961 TAGATCTATT TTGTTCAGTT TAAGACTTTA TTTGTCCGCC CACACCCGCT TTACGCAGGG  1021 CATCCATTTA TTACTCAACC GTAAACCGAT TTTGCCAGGC TACGCGGCTG GTCTATGCGT  1081 GGTGAAATAC GCACAGATGC GTAAGGGAGA AATACGCATC AGCGCCTCTT CGCTTCGTCG 1141 CTCACTTGAA CTTCCGCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl