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pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF (#RDB14530)

Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name Fxa_His- and Fxa_FLAG-tagged Human alpha1bata3 tubulin
Clone info. Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3. This clone is driven by the two promoters (PPH and Pp10) in a single vector for expression of two proteins simultaneously.
Comment Unpublished plasmid. Reference sequence: NP_006073 for TUBA1B, NP_006077 for TUBB3.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Gent^r.
Growth conditions LB+Amp, 37oC
Gene/insert name Human TUBA1B cDNA Human TUBB3 cDNA
Depositor|Developer Muto, Etsuko |

Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013)を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。

Catalog # Resource name Shipping form Fee
RDB14530 pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF DNA solution

How to cite this biological resource

Materials & Methods section:

The pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14530).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646. [PubMed] [Article] [RRC of NBRP]