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pEF/HRE-Fluc

HIF-1 dependent promoter reporter.

Catalog number RDB14404
Resource name pEF/HRE-Fluc
Alternative name HRE-Fluc
Clone info. HIF-1 dependent promoter reporter containing HRE of human VEGF sequence and firefly luciferase (HRE-Fluc).
Promoter region of pEF-1 and myc-tag were replaced with HRE-Fluc fragment. Expression was confirmed by the depositor with luciferase assay.
Vector backbone pEF6/Myc-His B plasmid (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human VEGF genomic DNA firefly (Photinus pyralis) luciferase cDNA
Depositor Kizaka-Kondoh, Shinae |
 
Other clones in our bank human VEGFA (NCBI Gene 7422) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016) RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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RDB14404 pEF/HRE-Fluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content HIF-1 signaling pathway (English text)

References

original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14404_A6Kpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R RDB14404_A6Kpa.seq
>14404_14404_A6Kp_2_Amp_R_E02_05_ABI08.ab1
    1 CTCCCGTTTT AATATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CAGATCGACG GATCGGGAGA TCGGTACCGA GCTTTTCTCG AGCCACAGTG
  181 CATACGTGGG CTCCAACAGG TCCTCTTGTC GAGCCACAGT GCATACGTGG GCTCCAACAG
  241 GTCCTCTTGT CGAGCCACAG TGCATACGTG GGCTCCAACA GGTCCTCTTG TCGAGCCACA
  301 GTGCATACGT GGGCTCCAAC AGGTCCTCTT GTCGAGCCAC AGTGCATACG TGGGCTCCAA
  361 CAGGTCCTCT TGTCGAGATC TGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT
  421 CGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC
  481 CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA TTCTATCCGC TGGAAGATGG
  541 AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT
  601 TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTAC GCTGAGTACT TCGAAATGTC
  661 CGTTCGGTTG GCAGAAGCTA TGAAACGATA TGGGCTGAAT ACAAATCACA GAATCGTCGT
  721 ATGCAGTGAA AACTCTCTTC AATTCTTTAT GCCGGTGTTG GGCGCGTTAT TTATCGGAGT
  781 TGCAGTTGCG CCCGCGAACG ACATTTATAA TGAACGTGAA TTGCTCAACA GTATGGGCAT
  841 TTCGCAGCCT ACCGTGGTGT TCGTTTCCAA AAAGGGGTTG CAAAAAATTT TGAACGTGCA
  901 AAAAAAGCTC CCAATCATCC AAAAAATTAT TATCATGGAT TCTAAAACGG ATTACCAGGG
  961 ATTTCAGTCG ATGTACACGT TCGTCACATC TCATCTACCT CCCGGGTTTT ATGATACGAT
 1021 TTTGTGCCAG AGGTCCTTCG ATAGGACAGA ACAATGCACT GATCATGAAC TTCTCTGGAT
 1081 CTACTGGATC TGCCTAGTGT CGCCTCTGGC TCATAGAACC TGGCCTTGGC CGTGAAGAAT
1141 TTCTT
//
Primer: BGH_rev2 RDB14404_A6Kpb.seq
>RDB14404_14404_A6Kp_2_BGH_rev2_E12_15_ABI24.ab1
    1 CTTTCAGGGT CAGGAGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGAG CTTCTAGAAT TACACGGCGA TCTTTCCGCC CTTCTTGGCC
  121 TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC TTTCGGTACT
  181 TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG ACTGGCGACG
  241 TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC AACGGCGGCG
  301 GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA GATGTTGGGG
  361 TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT GTACTTAATC
  421 AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA AGCTATGTCT
  481 CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG ATTGTTTACA
  541 TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT AACGCCCAGC
  601 GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC TTTACCGACC
  661 GCGCCCGGTT TATCATCCCC CTCGGGTGTA ATCAGAATAG CTGATGTAGT CTCAGTGAGC
  721 CCATATCCTT GCCTGATACC TGGCAGATGG AACCTCTTGG CAACCGCTTC CCCGACTTCC
  781 TTAGAGAGGG GAGCGCCACC AGAAGCAATT TCGTGTAAAT TAGATAAATC GTATTTGTCA
  841 ATCAGAGTGC TTTTGGCGAA GAAGGAGAAT AGGGTTGGCA CCAGCAGCGC ACTTTGAATC
  901 TTGTAATCCT GAAAGCTCCT CAGAAACAGC TCTTCTTCAA TCTATACATT AAGACGACTC
  961 GAAATCCACA TATCAATATC CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGAAC
 1021 AACACTTTAA AATCGCAGTA TCCGGAATGA TTTGATTTGC CCAAAATAGA TCTCTGGGCA
 1081 TGCGAGAATC TCACGCAGGC AGTTCTATTG AGCAGAGGCG ACAACCCTTT TTAAGGCCAG
1141 GAAC
//
Primer: SV40pro_F RDB14404_A6Kpc.seq
>RDB14404_14404_A6Kp_2_SV40pro_F_F12_18_ABI24.ab1
    1 CGCTTGGTCC GTACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCC CCCCGGGAGG TCCACAATGG TTGAACAAGA TGGATTGCAC GCAGGTTCTC
  121 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
  181 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
  241 ACCTGTCCGG TGCCCTGAAT GAACTCCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
  301 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
  361 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
  421 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
  481 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
  541 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
  601 CCAGGCTCAA GGCGCGTATG CCCGACGGCG AGGATCTCGT CGTGACTCAT GGCGATGCCT
  661 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
  721 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
  781 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
  841 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG
  901 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCC
  961 TTCTATGAAA AGGTTGGGGC TTCGGAAATC GTTTTCCCGG GACGCCGGGC TGGGATGATC
 1021 CTCCAGCGCG GGGATCTCAT GCTGGGAGTT CTTTCGCCAC CCCACTTTGT TTATTTGCAG
 1081 CCTTATAATG TACAAATAAA GCAATAGCCA TCACAAGTTT CACAAATAGC CATTTTTTTC
1141 AGTGCATCTA GTTGTGATTG TTCCCGAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl