RDB14404 - pEF/HRE-Fluc

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pEF/HRE-Fluc (#RDB14404)

HIF-1 dependent promoter reporter containing HRE.

Alternative name	HRE-Fluc
Clone info.	HIF-1 dependent promoter reporter containing HRE of human VEGF sequence and firefly luciferase (HRE-Fluc).
Comment	Promoter region of pEF-1 and myc-tag were replaced with HRE-Fluc fragment. Expression was confirmed by the depositor with luciferase assay.
Vector backbone	pEF6/Myc-His B plasmid (plasmid)
Size of vector backbone	5.8 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	human VEGF genomic DNA firefly (Photinus pyralis) luciferase cDNA
Depositor\|Developer	Kizaka-Kondoh, Shinae \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016).　RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016).　RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14404	pEF/HRE-Fluc	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF/HRE-Fluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14404).

Reference section:

Hoang, N.T., Kadonosono, T., Kuchimaru, T., Kizaka-Kondoh, S., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14404_A6Kpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R Sequence file: RDB14404_A6Kpa.seq
>14404_14404_A6Kp_2_Amp_R_E02_05_ABI08.ab1 1 CTCCCGTTTT AATATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 121 CACCTGACGT CAGATCGACG GATCGGGAGA TCGGTACCGA GCTTTTCTCG AGCCACAGTG 181 CATACGTGGG CTCCAACAGG TCCTCTTGTC GAGCCACAGT GCATACGTGG GCTCCAACAG 241 GTCCTCTTGT CGAGCCACAG TGCATACGTG GGCTCCAACA GGTCCTCTTG TCGAGCCACA 301 GTGCATACGT GGGCTCCAAC AGGTCCTCTT GTCGAGCCAC AGTGCATACG TGGGCTCCAA 361 CAGGTCCTCT TGTCGAGATC TGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 421 CGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC 481 CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA TTCTATCCGC TGGAAGATGG 541 AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT 601 TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTAC GCTGAGTACT TCGAAATGTC 661 CGTTCGGTTG GCAGAAGCTA TGAAACGATA TGGGCTGAAT ACAAATCACA GAATCGTCGT 721 ATGCAGTGAA AACTCTCTTC AATTCTTTAT GCCGGTGTTG GGCGCGTTAT TTATCGGAGT 781 TGCAGTTGCG CCCGCGAACG ACATTTATAA TGAACGTGAA TTGCTCAACA GTATGGGCAT 841 TTCGCAGCCT ACCGTGGTGT TCGTTTCCAA AAAGGGGTTG CAAAAAATTT TGAACGTGCA 901 AAAAAAGCTC CCAATCATCC AAAAAATTAT TATCATGGAT TCTAAAACGG ATTACCAGGG 961 ATTTCAGTCG ATGTACACGT TCGTCACATC TCATCTACCT CCCGGGTTTT ATGATACGAT 1021 TTTGTGCCAG AGGTCCTTCG ATAGGACAGA ACAATGCACT GATCATGAAC TTCTCTGGAT 1081 CTACTGGATC TGCCTAGTGT CGCCTCTGGC TCATAGAACC TGGCCTTGGC CGTGAAGAAT 1141 TTCTT //
Primer: BGH_rev2 Sequence file: RDB14404_A6Kpb.seq
>RDB14404_14404_A6Kp_2_BGH_rev2_E12_15_ABI24.ab1 1 CTTTCAGGGT CAGGAGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGAG CTTCTAGAAT TACACGGCGA TCTTTCCGCC CTTCTTGGCC 121 TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC TTTCGGTACT 181 TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG ACTGGCGACG 241 TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC AACGGCGGCG 301 GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA GATGTTGGGG 361 TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT GTACTTAATC 421 AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA AGCTATGTCT 481 CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG ATTGTTTACA 541 TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT AACGCCCAGC 601 GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC TTTACCGACC 661 GCGCCCGGTT TATCATCCCC CTCGGGTGTA ATCAGAATAG CTGATGTAGT CTCAGTGAGC 721 CCATATCCTT GCCTGATACC TGGCAGATGG AACCTCTTGG CAACCGCTTC CCCGACTTCC 781 TTAGAGAGGG GAGCGCCACC AGAAGCAATT TCGTGTAAAT TAGATAAATC GTATTTGTCA 841 ATCAGAGTGC TTTTGGCGAA GAAGGAGAAT AGGGTTGGCA CCAGCAGCGC ACTTTGAATC 901 TTGTAATCCT GAAAGCTCCT CAGAAACAGC TCTTCTTCAA TCTATACATT AAGACGACTC 961 GAAATCCACA TATCAATATC CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGAAC 1021 AACACTTTAA AATCGCAGTA TCCGGAATGA TTTGATTTGC CCAAAATAGA TCTCTGGGCA 1081 TGCGAGAATC TCACGCAGGC AGTTCTATTG AGCAGAGGCG ACAACCCTTT TTAAGGCCAG 1141 GAAC //
Primer: SV40pro_F Sequence file: RDB14404_A6Kpc.seq
>RDB14404_14404_A6Kp_2_SV40pro_F_F12_18_ABI24.ab1 1 CGCTTGGTCC GTACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCC CCCCGGGAGG TCCACAATGG TTGAACAAGA TGGATTGCAC GCAGGTTCTC 121 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 181 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 241 ACCTGTCCGG TGCCCTGAAT GAACTCCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 301 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 361 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 421 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 481 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 541 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 601 CCAGGCTCAA GGCGCGTATG CCCGACGGCG AGGATCTCGT CGTGACTCAT GGCGATGCCT 661 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 721 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 781 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 841 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG 901 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCC 961 TTCTATGAAA AGGTTGGGGC TTCGGAAATC GTTTTCCCGG GACGCCGGGC TGGGATGATC 1021 CTCCAGCGCG GGGATCTCAT GCTGGGAGTT CTTTCGCCAC CCCACTTTGT TTATTTGCAG 1081 CCTTATAATG TACAAATAAA GCAATAGCCA TCACAAGTTT CACAAATAGC CATTTTTTTC 1141 AGTGCATCTA GTTGTGATTG TTCCCGAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [link to RRC of NBRP]