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pGEX-6P-3/GST-TAT-ODD/3-0-Casp3

Bacterial expression vector of protein prodrug that is specifically processed and activated in hypoxia‐inducible factor‐active cells.

Catalog number RDB14403
Resource name pGEX-6P-3/GST-TAT-ODD/3-0-Casp3
Alternative name GST-TOP3
Clone info. Bacterial expression vector of GST tagged TAT-ODD-procaspase-3 (TOP3), a fusion protein prodrug containing TAT-PTD, ODD, and procaspase-3 specifically processed and activated in hypoxia‐inducible factor‐active cells. The recombinant protein showed prospective effects in hypoxic cells in vivo as well as in vitro.
Expression was confirmed by the depositor. cDNA fragment 2,046 to 2,216 of NM_001530.3 (HIF1A ODD/3-0) and 264 to 1,097 of NM_004346.3 (CASP3) were cloned. Known differences: T to C substitution at 876 of NM_004346.3 (S205P); T to C substitutuion at 1,061 of NM_004346.3 (V266, synonymous).
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human HIF1A cDNA human CASP3 cDNA
Depositor Kizaka-Kondoh, Shinae |
 
Other clones in our bank human HIF1A (NCBI Gene 3091) | human CASP3 (NCBI Gene 836) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016) RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

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RDB14403 pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14403_A6Kpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R RDB14403_A6Kpa.seq
>14403_14403_A6Kp_1_M13R_H03_08_ABI08.ab1
    1 GACTGTGGGC GGTCGGTTTT AAACGTCGTG ACTGGGAAAC CCTGGCGTTA CCCAACTTAA
   61 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
  121 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC
  181 ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT
  241 CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA
  301 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT
  361 CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG
  421 CGTTGGAATT AGCTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
  481 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
  541 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
  601 TGAAATGAGC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT
  661 AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG
  721 GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT
  781 TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG
  841 TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC
  901 ATACGTTATA TAGCTGACAG GCACAACATG TTGGGTGGTT TGTCCAAAGA GCGTGCAGAG
  961 ATTTCATGCT TGAAGGGAGC GGTTTGGGAT ATAGATACGT GTTTCGAGGA GTGCATATAG
 1021 TTAAGGACTT GCGCTCTCCA AAGTTGAATT TTCTAGCAAG CCTTACTTGG AATTGCCTGA
1081 ATTGTCGAGA TCGTTATGGT CATAAACATA GTTATAAATG GGTTGAT
//
Primer: pGEX-F2 RDB14403_A6Kpb.seq
>RDB14403_14403_A6Kp_1_pGEX-F2_A12_03_ABI24.ab1
    1 TCCTATTTGG TTTGGTTTTT AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTAACT
   61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
  121 GCGACCATCC TCCAAAATCG GATCTGGAAG TTCTGTTCCA GGGGCCCCTG GGATCCCCGA
  181 ATTCCCAGCT TGACATGGCG CCTAAGAAGA AGAGGAAGAG ATCATATGGT CGTAAGAAAC
  241 GTCGCCAACG TCGCCGAAGA TCTAACCCAT TTTCTACTCA GGACACAGAT TTAGACTTGG
  301 AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC TTCGATCAGT
  361 TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA AGCACAGTTA
  421 CAGTATTCCA GCAGGTACCA ATGGAGAACA CTGAAAACTC AGTGGATTCA AAATCCATTA
  481 AAAATTTGGA ACCAAAGATC ATACATGGAA GCGAATCAAT GGACTCTGGA ATATCCCTGG
  541 ACAACAGTTA TAAAATGGAT TATCCTGAGA TGGGTTTATG TATAATAATT AATAATAAGA
  601 ATTTTCATAA AAGCACTGGA ATGACATCTC GGTCTGGTAC AGATGTCGAT GCAGCAAACC
  661 TCAGGGAAAC ATTCAGAAAC TTGAAATATG AAGTCAGGAA TAAAAATGAT CTTACACGTG
  721 AAGAAATTGT GGAATTGATG CGTGATGTTT CTAAAGAAGA TCACAGCAAA AAGGAGCAGT
  781 TTTGTTTGTG TGCTTCTGAG CCATGGTGAA GAAGGAATAA TTTTTTGGAA CAAATGGACC
  841 TGTTGACCTG AAAAAAATAA CAAACTTTTT CAGAGGGGAT CGTTGTAGAA GTCTAACTGG
  901 AAAACCCAAA CTTTTCATTA TTCAGGCCTG CCGTGGTACA GAACTGGACT GTGGCATTGA
  961 GACAGACAGT GGTTGTTGAT GATGACATGG CGTGTCAATA AAAATACCAG GTGGAGGCCC
 1021 GACCTTCTTG TTATGCATAC TTCCCACCAG CACCTGGGTT ATTAATCCCT TTGGCGGAAA
 1081 TTCCAAAGGG ATTGGCTCCT GTCATCCCAG TTCGCTTGTG CCATGCTGAA CAGTATGCCG
1141 AACCAAGCCT TTTAAAATTT TTAATGTCAA CA
//
Primer: pGEX-R RDB14403_A6Kpc.seq
>14403_14403_A6Kp_1_pGEX-R_A03_03_ABI24.ab1
    1 TTCCATTTAC GGGAAAAGGC GCGAAGGCAG ATCGTCAGTC AGTCACGGAT GCGGCCGCTC
   61 GAGTCGACTT AGTGATAAAA ATAGAGTTCT TTTGTGAGCA TGGAGACAAT ACATGGAATC
  121 TGTTTCTTTG CATGAAAAGT AGCGTCAAAG GAAAAGGACT CAAATTCTGT TGCCACCTTT
  181 CGGTTAACCC GGGTAAGAAT GTGCATAAAT TCAAGCTTGT CGGCATACTG TTTCAGCATG
  241 GCACAAAGCG ACTGGATGAA CCAGGAGCCA TCCTTTGAAT TTCGCCAAGG ATAATAACCA
  301 GGTGCTGTGG AGTATGCATA CAAGAAGTCG GCCTCCACTG GTATTTTATG ACACGCCATG
  361 TCATCATCAA CACCACTGTC TGTCTCAATG CCACAGTCCA GTTCTGTACC ACGGCAGGCC
  421 TGAATAATGA AAAGTTTGGG TTTTCCAGTT AGACTTCTAC AACGATCCCC TCTGAAAAAG
  481 TTTGTTATTT TTTTCAGGTC AACAGGTCCA TTTGTTCCAA AAATTATTCC TTCTTCACCA
  541 TGGCTCAGAA GCACACAAAC AAAACTGCTC CTTTTGCTGT GATCTTCTTT AGAAACATCA
  601 CGCATCAATT CCACAATTTC TTCACGTGTA AGATCATTTT TATTCCTGAC TTCATATTTC
  661 AAGTTTCTGA ATGTTTCCCT GAGGTTTGCT GCATCGACAT CTGTACCAGA CCGAGATGTC
  721 ATTCCAGTGC TTTTATGAAA ATTCTTATTA TTAATTTATT ATACATAAAC CCAATCTCAG
  781 GATAATCCAT TTTATAAACT GTTGTCCAGG GATATTCCAG AGTCCATTGA TTCGCTTCCA
  841 TGTATGATCT TTGGGTTCCA AAATTTTTAA TGGATTTTGA ATCCACTGAA GTTTTCAGTG
  901 TTCTCCATTG GGTACCTGCT GGGAATACTG TAACTGTGCT TTGAGGACTT GCGCTTTCAA
  961 GGGCTTGTCG GAACTGCTTT TCTAATGGTG ACAACTTGAT CCAAGGAAAC CGTAAACTTG
 1021 GAAAGTCTTC CATCCCATTT GGGAATATAA GGAAGCCTAA ACATTCTCCC AAGTTCTAAA
 1081 TCCTGTGTCC CTTGAAGTAC AAAATGGGTT AGAATCTTTC TGCCGAACCT TGCCGCAACG
 1141 GATCTTACGA CCATATAGTG AATCCTCCTT CCTCCTTCCT GTCTAGGCCG ACCGCTCAAG
1201 TGTTCTACAG ATCTGATGGA GTA
//
Primer: M13_-40 RDB14403_A6Kpd.seq
>14403_14403_A6Kp_1_M13_-40_B03_06_ABI24.ab1
    1 ACCGCCAGTC ATCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
   61 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
  121 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
  181 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
  241 GCTAGCGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC
  301 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC
  361 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC
  421 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC
  481 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT
  541 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC
  601 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA
  661 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC
  721 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA
  781 GAGACATCAA AGAAATAACG CCGGAAACAT TAGTGCAGGC CAGCTTCCAA CAAGCAATGG
  841 CATCCCTGGT CATCCCAGCG GGATAGTTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA
901 AGATTGTGGC ACCGCCCGCT TTACAGGCTT CGACGCCGCC TTTCGTTCTA ACCATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl