Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 (#RDB14403)

Bacterial expression vector of protein prodrug TOP3 that is specifically processed and activated in hypoxia-inducible factor-active cells.

Alternative name	GST-TOP3
Clone info.	Bacterial expression vector of GST tagged TAT-ODD-procaspase-3 (TOP3), a fusion protein prodrug containing TAT-PTD, ODD, and procaspase-3 specifically processed and activated in hypoxia‐inducible factor‐active cells. The recombinant protein showed prospective effects in hypoxic cells in vivo as well as in vitro.
Comment	Expression was confirmed by the depositor. cDNA fragment 2,046 to 2,216 of NM_001530.3 (HIF1A ODD/3-0) and 264 to 1,097 of NM_004346.3 (CASP3) were cloned. Known differences: T to C substitution at 876 of NM_004346.3 (S205P); T to C substitutuion at 1,061 of NM_004346.3 (V266, synonymous).
Vector backbone	pGEX-6P-3 (plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	human HIF1A cDNA human CASP3 cDNA
Depositor|Developer	Kizaka-Kondoh, Shinae |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016).　RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14403	pGEX-6P-3/GST-TAT-ODD/3-0-Casp3	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14403).

Reference section:

Hoang, N.T., Kadonosono, T., Kuchimaru, T., Kizaka-Kondoh, S., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14403_A6Kpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R Sequence file: RDB14403_A6Kpa.seq
>14403_14403_A6Kp_1_M13R_H03_08_ABI08.ab1     1 GACTGTGGGC GGTCGGTTTT AAACGTCGTG ACTGGGAAAC CCTGGCGTTA CCCAACTTAA    61 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA   121 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC   181 ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT   241 CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA   301 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT   361 CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG   421 CGTTGGAATT AGCTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC   481 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC   541 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC   601 TGAAATGAGC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT   661 AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG   721 GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT   781 TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG   841 TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC   901 ATACGTTATA TAGCTGACAG GCACAACATG TTGGGTGGTT TGTCCAAAGA GCGTGCAGAG   961 ATTTCATGCT TGAAGGGAGC GGTTTGGGAT ATAGATACGT GTTTCGAGGA GTGCATATAG  1021 TTAAGGACTT GCGCTCTCCA AAGTTGAATT TTCTAGCAAG CCTTACTTGG AATTGCCTGA 1081 ATTGTCGAGA TCGTTATGGT CATAAACATA GTTATAAATG GGTTGAT //
Primer: pGEX-F2 Sequence file: RDB14403_A6Kpb.seq
>RDB14403_14403_A6Kp_1_pGEX-F2_A12_03_ABI24.ab1     1 TCCTATTTGG TTTGGTTTTT AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTAACT    61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG   121 GCGACCATCC TCCAAAATCG GATCTGGAAG TTCTGTTCCA GGGGCCCCTG GGATCCCCGA   181 ATTCCCAGCT TGACATGGCG CCTAAGAAGA AGAGGAAGAG ATCATATGGT CGTAAGAAAC   241 GTCGCCAACG TCGCCGAAGA TCTAACCCAT TTTCTACTCA GGACACAGAT TTAGACTTGG   301 AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC TTCGATCAGT   361 TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA AGCACAGTTA   421 CAGTATTCCA GCAGGTACCA ATGGAGAACA CTGAAAACTC AGTGGATTCA AAATCCATTA   481 AAAATTTGGA ACCAAAGATC ATACATGGAA GCGAATCAAT GGACTCTGGA ATATCCCTGG   541 ACAACAGTTA TAAAATGGAT TATCCTGAGA TGGGTTTATG TATAATAATT AATAATAAGA   601 ATTTTCATAA AAGCACTGGA ATGACATCTC GGTCTGGTAC AGATGTCGAT GCAGCAAACC   661 TCAGGGAAAC ATTCAGAAAC TTGAAATATG AAGTCAGGAA TAAAAATGAT CTTACACGTG   721 AAGAAATTGT GGAATTGATG CGTGATGTTT CTAAAGAAGA TCACAGCAAA AAGGAGCAGT   781 TTTGTTTGTG TGCTTCTGAG CCATGGTGAA GAAGGAATAA TTTTTTGGAA CAAATGGACC   841 TGTTGACCTG AAAAAAATAA CAAACTTTTT CAGAGGGGAT CGTTGTAGAA GTCTAACTGG   901 AAAACCCAAA CTTTTCATTA TTCAGGCCTG CCGTGGTACA GAACTGGACT GTGGCATTGA   961 GACAGACAGT GGTTGTTGAT GATGACATGG CGTGTCAATA AAAATACCAG GTGGAGGCCC  1021 GACCTTCTTG TTATGCATAC TTCCCACCAG CACCTGGGTT ATTAATCCCT TTGGCGGAAA  1081 TTCCAAAGGG ATTGGCTCCT GTCATCCCAG TTCGCTTGTG CCATGCTGAA CAGTATGCCG 1141 AACCAAGCCT TTTAAAATTT TTAATGTCAA CA //
Primer: pGEX-R Sequence file: RDB14403_A6Kpc.seq
>14403_14403_A6Kp_1_pGEX-R_A03_03_ABI24.ab1     1 TTCCATTTAC GGGAAAAGGC GCGAAGGCAG ATCGTCAGTC AGTCACGGAT GCGGCCGCTC    61 GAGTCGACTT AGTGATAAAA ATAGAGTTCT TTTGTGAGCA TGGAGACAAT ACATGGAATC   121 TGTTTCTTTG CATGAAAAGT AGCGTCAAAG GAAAAGGACT CAAATTCTGT TGCCACCTTT   181 CGGTTAACCC GGGTAAGAAT GTGCATAAAT TCAAGCTTGT CGGCATACTG TTTCAGCATG   241 GCACAAAGCG ACTGGATGAA CCAGGAGCCA TCCTTTGAAT TTCGCCAAGG ATAATAACCA   301 GGTGCTGTGG AGTATGCATA CAAGAAGTCG GCCTCCACTG GTATTTTATG ACACGCCATG   361 TCATCATCAA CACCACTGTC TGTCTCAATG CCACAGTCCA GTTCTGTACC ACGGCAGGCC   421 TGAATAATGA AAAGTTTGGG TTTTCCAGTT AGACTTCTAC AACGATCCCC TCTGAAAAAG   481 TTTGTTATTT TTTTCAGGTC AACAGGTCCA TTTGTTCCAA AAATTATTCC TTCTTCACCA   541 TGGCTCAGAA GCACACAAAC AAAACTGCTC CTTTTGCTGT GATCTTCTTT AGAAACATCA   601 CGCATCAATT CCACAATTTC TTCACGTGTA AGATCATTTT TATTCCTGAC TTCATATTTC   661 AAGTTTCTGA ATGTTTCCCT GAGGTTTGCT GCATCGACAT CTGTACCAGA CCGAGATGTC   721 ATTCCAGTGC TTTTATGAAA ATTCTTATTA TTAATTTATT ATACATAAAC CCAATCTCAG   781 GATAATCCAT TTTATAAACT GTTGTCCAGG GATATTCCAG AGTCCATTGA TTCGCTTCCA   841 TGTATGATCT TTGGGTTCCA AAATTTTTAA TGGATTTTGA ATCCACTGAA GTTTTCAGTG   901 TTCTCCATTG GGTACCTGCT GGGAATACTG TAACTGTGCT TTGAGGACTT GCGCTTTCAA   961 GGGCTTGTCG GAACTGCTTT TCTAATGGTG ACAACTTGAT CCAAGGAAAC CGTAAACTTG  1021 GAAAGTCTTC CATCCCATTT GGGAATATAA GGAAGCCTAA ACATTCTCCC AAGTTCTAAA  1081 TCCTGTGTCC CTTGAAGTAC AAAATGGGTT AGAATCTTTC TGCCGAACCT TGCCGCAACG  1141 GATCTTACGA CCATATAGTG AATCCTCCTT CCTCCTTCCT GTCTAGGCCG ACCGCTCAAG 1201 TGTTCTACAG ATCTGATGGA GTA //
Primer: M13_-40 Sequence file: RDB14403_A6Kpd.seq
>14403_14403_A6Kp_1_M13_-40_B03_06_ABI24.ab1     1 ACCGCCAGTC ATCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA    61 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG   121 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT   181 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC   241 GCTAGCGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC   301 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC   361 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC   421 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC   481 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT   541 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC   601 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA   661 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC   721 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA   781 GAGACATCAA AGAAATAACG CCGGAAACAT TAGTGCAGGC CAGCTTCCAA CAAGCAATGG   841 CATCCCTGGT CATCCCAGCG GGATAGTTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA 901 AGATTGTGGC ACCGCCCGCT TTACAGGCTT CGACGCCGCC TTTCGTTCTA ACCATC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [link to RRC of NBRP]

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Other resources in our bank

• human HIF1A
• human CASP3

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External Database
human HIF1A

• in KEGG Gene
• in NCBI Gene

human CASP3

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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Featured content

• HIF-1 signaling pathway (English text)

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