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pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 (#RDB14403)

Bacterial expression vector of protein prodrug TOP3 that is specifically processed and activated in hypoxia-inducible factor-active cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hoang, N.T., Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name GST-TOP3
Clone info. Bacterial expression vector of GST tagged TAT-ODD-procaspase-3 (TOP3), a fusion protein prodrug containing TAT-PTD, ODD, and procaspase-3 specifically processed and activated in hypoxia‐inducible factor‐active cells. The recombinant protein showed prospective effects in hypoxic cells in vivo as well as in vitro.
Comment Expression was confirmed by the depositor. cDNA fragment 2,046 to 2,216 of NM_001530.3 (HIF1A ODD/3-0) and 264 to 1,097 of NM_004346.3 (CASP3) were cloned. Known differences: T to C substitution at 876 of NM_004346.3 (S205P); T to C substitutuion at 1,061 of NM_004346.3 (V266, synonymous).
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human HIF1A cDNA human CASP3 cDNA
Depositor|Developer Kizaka-Kondoh, Shinae |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016).　RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。


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RDB14403 pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 DNA solution

How to cite this biological resource

Materials & Methods section:

The pGEX-6P-3/GST-TAT-ODD/3-0-Casp3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14403).

Reference section:

Hoang, N.T., Kadonosono, T., Kuchimaru, T., Kizaka-Kondoh, S., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607. [PubMed] [Article] [RRC of NBRP]