Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-V4 (#RDB14374)

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-4 (Nucleotide sequence accession number: AB743887). pCoGFP-V4 is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of insert sequence AB743887 (DDBJ accession number)
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Ogoh, K. Luminescence 28 (4): 582-591 (2013))。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

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RDB14374 pCoGFP-V4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-V4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14374).

Reference section:

Ogoh, K., Kinebuchi, T., Murai, M., Takahashi, T., Ohmiya, Y., Suzuki, H., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14374_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14374_A6Jca.seq check
>D03707B7_A6Jc_1_Reverse2_H05_23_ABI24.ab1
    1 CGTTAAATCA ATTACGGCCA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG ATCCGATGAG
   61 CATCCCCGAG AACAGCGGCC TGACCGAAGA GATGCCCGCC CAGATGAACC TGGAAGGGGT
  121 GGTCAACGGC CACGCCTTCT CCATGGAAGG CATCGGCGGA GGCAACATCC TGACCGGCAT
  181 CCAGAAACTG GACATCAGAG TGATCGAGGG CGACCCCCTG CCCTTCAGCT TTGACATCCT
  241 GAGCGTGGCC TTCCAGTACG GCAACCGGAC CTACACCAGC TACCCCGCCA AGATCCCCGA
  301 CTACTTCGTG CAGAGCTTCC CCGAGGGCTT CACCTTCGAG CGGACCCTGA GCTTTGAGGA
  361 CGGCGCCATC GTGAAGGTGG AAAGCGACAT CAGCATCGAG GACGGCAAGT TCGTGGGCAA
  421 GATCAAGTAC AACGGCGAGG GCTTCCCAGA GGACGGCCCC GTGATGAAGA AAGAAGTGAC
  481 CAAGCTGGAA CCCGCCAGCG AGTCTATGTA CGTGAGCGAC GGCACCCTCG TGGGCGAGGT
  541 GGTGCTGAGC TACAAGACCC AGAGCACCCA CTACACCTGT CACATGAAGA CCATCTACCG
  601 GTCCAAGAAA CCCGTGGAAA ACCTGCCCAA GTTCCACTAC GTGCACCACC GGCTCGAAAA
  661 GAAAATCGTC GAAGAGGGCT ACTACTACGA GCAGCACGAG ACAGCCATTG CCAAGCCCTG
  721 AGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
  781 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
  841 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG ATGCGGTATT
  901 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT
  961 GCTCTGATGC CCGCATAGTT AAGCCCAGCC CCCGACACCC GCCCAACACC CGGCTTGACG
 1021 GCCGCCCTGA CGGCTTGTCT GGCTTCCGGC CATTCGCCTT ACAGACAGCC TGTGAACGGT
1081 CTCCGGAAGC TGGCATGGTG GTTCCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]