Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-V3 (#RDB14373)

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-3 (Nucleotide sequence accession number: AB743886). pCoGFP-V3 is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of insert sequence AB743886 (DDBJ accession number)
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Ogoh, K. Luminescence 28 (4): 582-591 (2013))。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14373 pCoGFP-V3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-V3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14373).

Reference section:

Ogoh, K., Kinebuchi, T., Murai, M., Takahashi, T., Ohmiya, Y., Suzuki, H., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14373_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14373_A6Jca.seq check
>D03707B6_A6Jc_1_Reverse2_F05_17_ABI24.ab1
    1 CGGTAAAGGA ATTACGCCAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCGATGAGC
   61 ATCCCCGAGA ACAGCGGCCT GACCGAAGAG ATGCCCGCCC AGATGAACCT GGAAGGGGTG
  121 GTCAACGGCC ACGCCTTCTC CATGGAAGGC ATCGGCGGAG GCAACATCCT GACCGGCATC
  181 CAGAAACTGG ACATCAGAGT GATCGAGGGC GACCCCCTGC CCTTCAGCTT TGACATCCTG
  241 AGCGTGGCCT TCCAGTACGG CAACCGGACC TACACCAGCT ACCCCGCCAA GATCCCCGAC
  301 TACTTCGTGC AGAGCTTCCC CGAGGGCTTC ACCTTCGAGC GGACCCTGAG CTTTGAGGAC
  361 GGCGCCATCG TGAAGGTGGA AAGCGACATC AGCATCGAGG ACGGCAAGTT CGTGGGCAAG
  421 ATCAAGTACA ACGGCGAGGG CTTCCCAGAG GACGGCCCCG TGATGAAGAA AGAAGTGACC
  481 AAGCTGGAAC CCTGCAGCGA GTCTATGTAC GTGAGCGACG GCACCCTCGT GGGCGAGGTG
  541 GTGCTGAGCT ACAAGACCCA GAGCACCCAC TACACCTGTC ACATGAAGAC CATCTACCGG
  601 TCCAAGAAAC CCGTGGAAAA CCTGCCCAAG TTCCACTACG TGCACCACCG GCTCGAAAAG
  661 AAAATCGTCG AAGAGGGCTA CTACTACGAG CAGCACGAGA CAGCCATTGC CAAGCCCTGA
  721 GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
  781 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
  841 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT
  901 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG
  961 CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCC AACACCCGCT GACGCGCCCT
 1021 GACGGGGCTT GTCTGCCTCC GGCCATCCGC TTACAGACAG CCTGTGAACC GGATCTCCGG
1081 GAGCTTGCAT GGGTGATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]