Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-wt (#RDB14368)

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Wild-type (Nucleotide sequence accession number: AB743881). pCoGFP-wt is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of insert sequence AB743881 (DDBJ accession number)
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (Ogoh, K. Luminescence 28 (4): 582-591 (2013))。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

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RDB14368 pCoGFP-wt DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14368).

Reference section:

Ogoh, K., Kinebuchi, T., Murai, M., Takahashi, T., Ohmiya, Y., Suzuki, H., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14368_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100
Sequence file: RDB14368_A6Jca.seq check
>D03707B1_A6Jc_2_pCU_M13-100_C02_08_ABI24.ab1
    1 GACGGTAAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA ATTCGAGCTC GGTACCTCAT GGTTTAGCTA TAGCCGTCTC ATGTTGCTCG
  121 TAGTAGTTGC CTTCCTCGAC TTTTTTCTTT TCGAGGCGAT GATGAACATA ATGGAACTTT
  181 GGCAGGTTTT CTACTGGCTT TTTGGACCTG TAGATTGTTT TCATATGGCA AGTGTAATGA
  241 GTCGACTGGG TCTTGTAAGA CAACACAACT TCTCCCACCA AAGTGTCGTC ACATACATAC
  301 ATTGACTCAC TGGACGGTTC TAGTTTGGTA ACTTCCTTTT TCATCACCGG TCCATCCTCT
  361 GGAAAACTTT CGCCATTATA TTTAATCTTG CCAACAAATT TGCCATCTTC AATACTAATA
  421 TCCGATTCGA CCTTTACGAT TGCACCATCT TCAAAACTAA GAGTCCTTTC AAAGGTGAAC
  481 CCTTCTGGGA ATGATTGAAC GAAATAATCG GGGATCTTCG CAGGATAACT TGTGTAAGTT
  541 CTGTTGCCAT ATTGAAACGC AACTGAGAGA ATGTCAAAGG AAAACGGTAG AGGATCCCCC
  601 TCGATGACAC GGATATCCAG CTTTTGAATT CCAGTTAGTA TGTTCCCTCC ACCAATTCCT
  661 TCCATAGAGA AGGCATGACC ATTTACCACT CCTTCTAAAT TCATTTGAGC AGGCATCTCT
  721 TCTGTTAAGC CCGAATTCTC TGGAATACTC ATAAGCTTGG CGTAATCATG GTCATAGCTG
  781 TTTCCTGTGT GAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA
  841 AGTGTAAAGC CTGGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA
  901 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTTAATGAA ATCGGCCAAC
  961 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCCTCGCT CACTGACTCG
 1021 CTGCGCTCGG TCGGTTCGGC TGCGGGCGAG CGGTATCCAG CCTCACTCAA AGGCGGTAAT
1081 ACGCTAATCA CAGGAGTCAA GGGGATTACG TCAAGGAAAA GGAATCCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077. [link to RRC of NBRP]