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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-Th1co (#RDB14365)

Thermostable green fluorescent protein of sea cactus Cavernularia obesa.

Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1 codon optimized. pCoGFP-Th1co is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent publication 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-60947)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14365 pCoGFP-Th1co DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-Th1co was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14365).

Reference section:

Murai, M., Ogoh, K., Kinebuchi, T., Suzuki, H., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14365_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14365_A6Jca.seq check
>D03707A7_A6Jc_2_Reverse2_F01_16_ABI24.ab1
    1 CGTACTCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGAGCATC
   61 CCCGAGAACA GCGGCCTGAC CGAAGAGATG CCCGCCCAGA TGAACCTGGA AGGGGTGGTC
  121 AACGGCCACG CCTTCTCCAT GGAAGGCATC GGCGGAGGCA ACATCCTGAC CGGCATCCAG
  181 AAACTGGACA TCAGAGTGAC CGAGGGCGAC CCCCTGCCCT TCAGCTTTGA CATCCTGAGC
  241 GTGGCCTTCC AGTACGGCAA CCGGACCTAC ACCAGCTACC CCGCCAAGAT CCCCGACTAC
  301 TTCGTGCAGA GCTTCCCCGA GGGCTTCACC TTCGAGCGGA CCCTGAGCTT TGAGGACGGC
  361 GCCATCGTGA AGGTGGAAAG CGACATCAGC ATCGAGGACG GCAAGTTCGT GGGCAAGATC
  421 AAGTACAACG GCGAGTCCTT CCCAGAGGAC GGCCCCGTGA TGAAGAAAGA AGTGATCAAG
  481 CTGGAACCCA GCAGCGAGTC TATGTACGTG CGCGACGACA CCCTCGTGGG CGAGGTGGTG
  541 CTGAGCTACA AGACCCAGAG CACCCACTAC ACCTGTCACA TGAAGACCAT CTACCGGTCC
  601 AAGAAACCCG TGGAAAACCT GCCCAAGTTC CACTACGTGC ACCACCGGCT CGAAAAGAAA
  661 AAGGTCGAAG AGGGCAACTA CTACGAGCAG CACGAGACAG CCATTGCCAA GCCCTGAGAA
  721 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
  781 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
  841 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
  901 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
  961 TGATGCCGCA TAGTTTAAGC CAGCCCCCGA CACCCGCCCA ACACCCGCTG ACGCGCCCTG
 1021 ACGGGCTTGT CTGCTCCCGG GCATCCGGCT TACAGACGAG CTGTGACCGT CTCGGTAGCC
1081 TGCCATGTGT CAGAGGTTTC ACCGCTCATC ACTGAACTCG CTGCGTAGGA TACCCGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]