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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-Th1wt (#RDB14364)

Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1.

Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1. pCoGFP-Th1wt is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent publication 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-60947)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14364 pCoGFP-Th1wt DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCoGFP-Th1wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14364).

Reference section:

Murai, M., Ogoh, K., Kinebuchi, T., Suzuki, H., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14364_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100
Sequence file: RDB14364_A6Jca.seq check
>D03707A6_A6Jc_1_pCU_M13-100_C01_07_ABI24.ab1
    1 GTATGTCAAG GCGGATTAAG TTGGGTACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
   61 CGACGGCCAG TGAATTCGAG CTCGGTACCC GGGGATCCAT GAGTATTCCA GAGAATTCGG
  121 GCTTAACAGA AGAGATGCCT GCTCAAATGA ATTTAGAAGG AGTGGTAAAT GGTCATGCCT
  181 TCTCTATGGA AGGAATTGGT GGAGGGAACA TACTAACTGG AATTCAAAAG CTGGATATCC
  241 GTGTCACCGA GGGAGATCCT CTTCCGTTTT CCTTTGACAT TCTCTCAGTT GCGTTTCAAT
  301 ATGGCAACAG AACTTACACA AGTTATCCTG CGAAGATCCC CGATTATTTC GTTCAATCAT
  361 TCCCAGAAGG GTTCACCTTT GAAAGGACTC TTAGTTTTGA AGATGGTGCA ATCGTAAAGG
  421 TCGAATCGGA TATTAGTATT GAAGATGGCA AATTTGTTGG CAAGATTAAA TATAATGGCG
  481 AAAGTTTTCC AGAGGATGGA CCGGTGATGA AAAAGGAAGT TATCAAACTA GAACCGTCCA
  541 GTGAGTCAAT GTATGTACGT GACGACACTT TGGTGGGAGA AGTTGTGTTG TCTTACAAGA
  601 CCCAGTCGAC TCATTACACT TGCCATATGA AAACAATCTA CAGGTCCAAA AAGCCAGTAG
  661 AAAACCTGCC AAAGTTCCAT TATGTTCATC ATCGCCTCGA AAAGAAAAAA GTCGAGGAAG
  721 GCAACTACTA CGAGCAACAT GAGACGGCCA TAGCTAAACC ATGAAAGCTT GGCGTAATCA
  781 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
  841 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
  901 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCCAGC TGCATTAATG
  961 AATCGGCCAA CGCGCGGGGG AGAGGCGGTT TGCGTATTGG GCCGCTCCTT CCGCCTTCCT
 1021 CGCTCACTGA CCTCGCCTGC CGCCTCCGGT CCGTTCCGGC CTGCCGCCGA GCCGATATCC
 1081 AAGCCTCCCA ACTCCAAGCC GTATACCGGT TTATTCCCAC ACAGAAGTTC CAGGGGGGGA
1141 TAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]