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pSflaRE1Luc

Red-shifted luciferase of Stenocladius flavipennis firefly with higher luminous intensity.

Catalog number RDB14362
Resource name pSflaRE1Luc
Clone info. Red-shifted luciferase of Stenocladius flavipennis firefly with higher luminous intensity. A maximum luminescent wavelength falls 613 nm (Fig. 9 of Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-81459). pSflaRE1Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Stenocladius flavipennis Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
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External Database
Stenocladius flavipennis Luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-81459). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ 特開2013-81459)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14362 pSflaRE1Luc DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-81459.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14362_A6Jcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB14362_A6Jca.seq check
          >D03707A4_A6Jc_3_Reverse2_H04_22_ABI24.ab1
              1 AGGGAAGGCA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCGATGGCC
             61 AGCAGCATGA TGAGCAAGAA GGACCTGGAA GATAAGAACG TGGTGCACGG CCCCGACCCC
            121 TACTACCTGG TGGATGAGGG CAATGCCGGC CAGCAGCTGC ACAAGACCAT CCTGAGATAC
            181 GCCCAGCTGC CCGACACAAT CGCCTTCACC GACGGCCACA CCAAGCGGGA TGTGACCTAC
            241 GCCCACTACT TCGACCTGAC CTGCAGACTG GCCGAGAGCC TGAAGAGATA CGGCCTGAAC
            301 CTGCAGAGCC GGATCGCCGT GTGCAGCGAG AACAACGTGG AATTTTTCAT CCCCGTGGTG
            361 GCCAGCCTGT ACCTGGGAGT GGGAGTGGCC CCCACCAACG ACATCTACAA CGAGACAGAG
            421 CTGTTCAACA GCCTGAACAT CAGCCAGCCC ACCATCGTGT TCGTGTCCAA GCGGGCCCTG
            481 CACAAGATCC TGGAAGTGAA GAAGCGCATC CCCATCATCA AGACCGTGGT GGTGCTGGAC
            541 ACCGAAGAGG ACTTCATGGG CTACCACTGC CTGCACAGCT TTATGAAGCA CTACCTGCCC
            601 CCCAACTTCG ACATCATGAG CTACAAGCCC GAAGAGTTCG CCCGGGATGG ACAGCTGGCC
            661 CTGATCATGA ACAGCAGCGG CAGCACCGGC CTGCCTAAAG GCGTGATGCT GGCCCACAGA
            721 TCCGTGGTCG TGCGGTTCAG CCACTGCAAG GACCCCGTGT TCGGCAACCA GATCATCCCC
            781 GACACCGCTA TCCTGACCGT GATCCCTTTC CACCACGGCT TCGGCATGTT CACCACCCTG
            841 GGCTACCTGA CCTGTGGCTT CCGGATCGTG CTGCTGCGGA AGTTCGACGA GCACTACTTT
            901 CTGAAGTGCC TGCAGGACTA CAAGATCCAG TTTGCCCTGC TGGGTGCCCT ACCCTGTTTC
            961 AGCTTTCTTT CGCCAAGAGC ACCCTGTGGA CCAGTACGAC CTGAGCAACC CTGAAAGAGA
           1021 TCGGCAGCGG GCGGGAGCCC CCCCTTGCTA AGGAGTGGGG AGAGGTCGTC GCCAGCGGCT
          1081 TTAAGCCTGC CCGGTCATTC CCGA
          //
          Primer: pUC-M13_-100
          Sequence file: RDB14362_A6Jcb.seq check
          >D03707A4_A6Jc_3_pUC-M13_-100_G04_19_ABI24.ab1
              1 GACTTTCAAG CGGATAAGGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGTTTCTT CAGGATTTCT TTCAGGGCCT
            121 TCCGGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCATC GATGAAGCGC ACACCGCCTC
            181 TCAGTCTCTT GTAGCAGCTC AGCTGGCCGG CGACGTAGTC CATCACTTCC TGCTCGGTCA
            241 CGTGCTTGCC CTTTTCCAGG ACCACACAGG CGCCAGGCAG TTCGCCGTCC ACATCATCTG
            301 GCACGCCGGT CACGCCGGCA TCGAAGATGC AGGGATGCTG CAGCAGCACA GCTTCCAGTT
            361 CGGCAGGGGC CACCTGGTAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
            421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCAA CGTCGCCGGA GTGCAGCCAG CCATCCTTGT
            481 CGATCATCTC GTCGGTGGCG GCCTTGTTGT TGCAGTAGCC CATCATGATC ATGTCGCCCT
            541 TCAGGTAGAG TTCTCCCCTC TGGTGAGGGC CCACGCTCTT GCCGCTGTTC AGGTCCACGA
            601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC AGGCTTATCC TCGCCCTCGG
            661 GGGTGATGAT CACGGCGCTG GTTGTCTCGG TCAGGCCGTA GCCCTGTCTG ATGCCGGGCA
            721 GCTTAAACCG CTTGGCGACG GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCTGG
            781 CGATCTCTTT CAGGTTGCTC AGGTCGTACT GGTCCACCAG GGTGCTCTTG GCGAAGAAGC
            841 TGAACAGGGT AGGCACCAGC AGGGCAAACT GGATCTTGTA GTCCTGCAGG CACTTCAGAA
            901 AGTAGTGCTC GTCGAACTTC CGCAGCAGCA CGATCCGGGA AGCCCACAGG TCAGGTAGCC
            961 CAGGGTGGTG ACATGCCGAA GCCGTTGTTG GAAAGGATCA CGTCAGATAG CGTGTCGGGG
           1021 ATGATCTGCT TGCCCGACAC GGTTCCCTGC CAGTGACCTG AATCGCCACG ACCCACCCGA
          1081 TCTGGTGGGC CCAAGCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-81459.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl