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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSflaRE1Luc (#RDB14362)

Red-shifted luciferase of Stenocladius flavipennis firefly with higher luminous intensity.

Clone info. Red-shifted luciferase of Stenocladius flavipennis firefly with higher luminous intensity. A maximum luminescent wavelength falls 613 nm (Fig. 9 of Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-81459). pSflaRE1Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Stenocladius flavipennis Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh K, Akiyoshi R, Suzuki H. 2013. Firefly luciferase. Japan patent publication 2013-81459). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2013-81459)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14362 pSflaRE1Luc DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSflaRE1Luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14362).

Reference section:

Ogoh, K., Akiyoshi, R., Suzuki, H., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
Ogoh, K., Akiyoshi, R., Suzuki, H., Firefly luciferase. Japan Patent Abstracts 2013-81459. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14362_A6Jcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB14362_A6Jca.seq check
>D03707A4_A6Jc_3_Reverse2_H04_22_ABI24.ab1
    1 AGGGAAGGCA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCGATGGCC
   61 AGCAGCATGA TGAGCAAGAA GGACCTGGAA GATAAGAACG TGGTGCACGG CCCCGACCCC
  121 TACTACCTGG TGGATGAGGG CAATGCCGGC CAGCAGCTGC ACAAGACCAT CCTGAGATAC
  181 GCCCAGCTGC CCGACACAAT CGCCTTCACC GACGGCCACA CCAAGCGGGA TGTGACCTAC
  241 GCCCACTACT TCGACCTGAC CTGCAGACTG GCCGAGAGCC TGAAGAGATA CGGCCTGAAC
  301 CTGCAGAGCC GGATCGCCGT GTGCAGCGAG AACAACGTGG AATTTTTCAT CCCCGTGGTG
  361 GCCAGCCTGT ACCTGGGAGT GGGAGTGGCC CCCACCAACG ACATCTACAA CGAGACAGAG
  421 CTGTTCAACA GCCTGAACAT CAGCCAGCCC ACCATCGTGT TCGTGTCCAA GCGGGCCCTG
  481 CACAAGATCC TGGAAGTGAA GAAGCGCATC CCCATCATCA AGACCGTGGT GGTGCTGGAC
  541 ACCGAAGAGG ACTTCATGGG CTACCACTGC CTGCACAGCT TTATGAAGCA CTACCTGCCC
  601 CCCAACTTCG ACATCATGAG CTACAAGCCC GAAGAGTTCG CCCGGGATGG ACAGCTGGCC
  661 CTGATCATGA ACAGCAGCGG CAGCACCGGC CTGCCTAAAG GCGTGATGCT GGCCCACAGA
  721 TCCGTGGTCG TGCGGTTCAG CCACTGCAAG GACCCCGTGT TCGGCAACCA GATCATCCCC
  781 GACACCGCTA TCCTGACCGT GATCCCTTTC CACCACGGCT TCGGCATGTT CACCACCCTG
  841 GGCTACCTGA CCTGTGGCTT CCGGATCGTG CTGCTGCGGA AGTTCGACGA GCACTACTTT
  901 CTGAAGTGCC TGCAGGACTA CAAGATCCAG TTTGCCCTGC TGGGTGCCCT ACCCTGTTTC
  961 AGCTTTCTTT CGCCAAGAGC ACCCTGTGGA CCAGTACGAC CTGAGCAACC CTGAAAGAGA
 1021 TCGGCAGCGG GCGGGAGCCC CCCCTTGCTA AGGAGTGGGG AGAGGTCGTC GCCAGCGGCT
1081 TTAAGCCTGC CCGGTCATTC CCGA
//
Primer: pUC-M13_-100
Sequence file: RDB14362_A6Jcb.seq check
>D03707A4_A6Jc_3_pUC-M13_-100_G04_19_ABI24.ab1
    1 GACTTTCAAG CGGATAAGGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGTTTCTT CAGGATTTCT TTCAGGGCCT
  121 TCCGGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCATC GATGAAGCGC ACACCGCCTC
  181 TCAGTCTCTT GTAGCAGCTC AGCTGGCCGG CGACGTAGTC CATCACTTCC TGCTCGGTCA
  241 CGTGCTTGCC CTTTTCCAGG ACCACACAGG CGCCAGGCAG TTCGCCGTCC ACATCATCTG
  301 GCACGCCGGT CACGCCGGCA TCGAAGATGC AGGGATGCTG CAGCAGCACA GCTTCCAGTT
  361 CGGCAGGGGC CACCTGGTAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
  421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCAA CGTCGCCGGA GTGCAGCCAG CCATCCTTGT
  481 CGATCATCTC GTCGGTGGCG GCCTTGTTGT TGCAGTAGCC CATCATGATC ATGTCGCCCT
  541 TCAGGTAGAG TTCTCCCCTC TGGTGAGGGC CCACGCTCTT GCCGCTGTTC AGGTCCACGA
  601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC AGGCTTATCC TCGCCCTCGG
  661 GGGTGATGAT CACGGCGCTG GTTGTCTCGG TCAGGCCGTA GCCCTGTCTG ATGCCGGGCA
  721 GCTTAAACCG CTTGGCGACG GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCTGG
  781 CGATCTCTTT CAGGTTGCTC AGGTCGTACT GGTCCACCAG GGTGCTCTTG GCGAAGAAGC
  841 TGAACAGGGT AGGCACCAGC AGGGCAAACT GGATCTTGTA GTCCTGCAGG CACTTCAGAA
  901 AGTAGTGCTC GTCGAACTTC CGCAGCAGCA CGATCCGGGA AGCCCACAGG TCAGGTAGCC
  961 CAGGGTGGTG ACATGCCGAA GCCGTTGTTG GAAAGGATCA CGTCAGATAG CGTGTCGGGG
 1021 ATGATCTGCT TGCCCGACAC GGTTCCCTGC CAGTGACCTG AATCGCCACG ACCCACCCGA
1081 TCTGGTGGGC CCAAGCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ogoh, K., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
original Ogoh, K., Firefly luciferase. Japan Patent Abstracts 2013-81459. [link to RRC of NBRP]
review Suzuki, H., Bioluminescence microscopy for the visualization of gene expression patterns and the study of embryonic development. Proc. Arthropod. Embryol. Soc. Jpn. 53: 1-7 (2021).