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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBabe-hSTING(N154S_C88S_C91S) (#RDB14357)

Retroviral expression vector of human STING, N154S_C88S_C91S mutant.

Clone info. Mammalian retroviral vector for expressing human STING (mutation, N154S. dual mutation, C88S/C91S). Suppression of STING palmitoylation inhibits the gain-of-function phenotype in the SAVI–STING.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14357 pBabe-hSTING(N154S_C88S_C91S) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(N154S_C88S_C91S) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14357).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14357_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14357_A6J7a.seq check
>D03866A7_A6J7_1_pBABE5'_G01_19_ABI24.ab1
    1 TGTGGCCGGA ATCCAGGTGG TGGGTGGTAC GTAGGATTCG CCGCCACCAT GCCCCACTCC
   61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT
  121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC
  181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC
  241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT
  301 GTGCGGGCCA GCCTGGGCAG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT
  361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG
  421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC
  481 TCTGCAGTGT GTGAAAAAGG GAATTTCAGC GTGGCCCATG GGCTGGCATG GTCATATTAC
  541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG
  601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG
  661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA ACATTCGCTT CCTGGATAAA
  721 CTGCCCCAGC AGACCGGTGA CCATGCTGGC ATCAAGGATC GGGTTTACAG CAACAGCATC
  781 TATGAGCTTC TGGAGAACGG GCAGCGGGCG GGCACCTGTG TCCTGGAGTA CGCCACCCCC
  841 TTGCAGACTT TGTTTGCCAT GTCACAAATA CAGTCAAGCT GGCTTTAGCC GGGGAGGATA
  901 GGCTTGAGCA GGCCAAACTC TTCTGCCCGG ACACTTTGAG GACATCCCTG GCAGATGCCC
  961 CCTGAAGTCT CAGAACAACT GCCGCCTCAT TTGCCTACCA GGAAACCTTG CAGATGACAG
 1021 CAGCTTCTTC GCTGTCCAAG GAGGATCTCC GGCACCCTTG CCGGCAGGAG AAAAAGGAAG
 1081 AAGGTTAACT GGTGGGCCAG CCTGGAGGAC CTTCAGCGGG TGGCCGCAAG TATTCCTCTC
1141 CTACCGAATG
//
Primer: PuroR-rev ()
Sequence file: RDB14357_A6J7b.seq check
>D03866A7_A6J7_1_PuroR-rev_G06_07_ABI08.ab1
    1 GTTGGGCTTT TTGCAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC
   61 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC
  121 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT
  181 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
  241 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT
  301 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGGGT CGAGTCAAGA
  361 GAAATCCGTG CGGAGAGGGA GGGGCTTTTC CATTCCACTG ATGAGGAGCT CAGGCTCTTG
  421 GGACATCGTG GAGGTACTGG GCACCGCTGA GGTCTTCAAG CTGCCCACAG TAACCTCTTC
  481 CTTTTCCTCC TGCCGCAGGT GCCGGAGAAC CTCCTGGGAC AGCGAGAAGC TGCTGTCATC
  541 TGCAGGTTCC TGGTAGGCAA TGAGGCGGCA GTTGTTCTGA GACTCAGGGG CATCTGCCAG
  601 GATGTCCTCA AGTGTCCGGC AGAAGAGTTT GGCCTGCTCA AGCCTATCCT CCCGGCTAAA
  661 GCCAGCTTGA CTGTATTGTG ACATGGCAAA CAAAGTCTGC AAGGGGGTGG CGTACTCCAG
  721 GACACAGGTG CCCGCCCGCT GCCCGTTCTC CAGAAGCTCA TAGATGCTGT TGCTGTAAAC
  781 CCGATCCTTG ATGCCAGCAT GGTCACCGGT CTGCTGGGGC AGTTTATCCA GGAAGCGAAT
  841 GTTGGGGTCA GCCATACTCA GGTTATCAGG CACCCCACAG TCCAATGGGA GGAGAATATA
  901 CAGCCGCTGG CTCACTGCAC CCCGTAGCAG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
  961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCG CAGATATCCG ATGTAATATG ACCATGCCCA
 1021 GCCCATGGGC CACGCTGAAA TTTCCCTTTT TTCACACACT GCAGAGATCT CAGCTGGGGC
 1081 AGTCTTGGAG CCCAGAGATG TCATGACTGC TAGAGGCCAG GAGGCAAGCC ATTCTAGTGA
1141 AGGGCCGGGG C
//
Primer: Amp_R ()
Sequence file: RDB14357_A6J7c.seq check
>D03866A7_A6J7_1_Amp_R_G03_21_ABI24.ab1
    1 ACTTTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCAGCC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  181 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  241 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  301 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  361 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  421 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG
  481 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  541 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  601 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  661 GGCAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  721 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  781 AACTGACGAG TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG
  841 CCGTTTTTGT GGCCCGACCT GAGGAAGGGA GTCGATGTGG GAATCCGACC CCGTCAGGAT
  901 ATGTGGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCCTCCGT CTGAATTTTT
  961 TGCTTTCGGT TTGGAACCGA AGCCGGCGCG TCTTGTCTGC TGCAGCGCTG CAGCATCGTT
 1021 CTGTGTTGTC TCTGTTCTGA CTGTGTTTCT GTATTGTCTG AATTAGGCCC AGACTGGTAA
1081 CCCACCTCCT TAGTTGAACT TAGTTCACCT GAAAGATGGT TTCGAGAGCC GGGAAT
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14357_A6J7d.seq check
>D03866A7_A6J7_1_SV40pro_F_V2_G05_20_ABI24.ab1
    1 GTGCGGCCTT TTTGGGAGGC CTAGGCTTTT GCAAAAGCTT ACCATGACCG AGTACAAGCC
   61 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  121 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  181 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  241 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  301 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  361 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  421 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  481 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  541 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  601 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  661 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATAAAATAAA AGATTTTATT
  721 TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA
  781 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC
  841 AAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGGTAAGC
  901 AGTTCCTGCC CCGGGCTCAG GGCCCAAGAA CAGATGGGAA CAGCTGAAAT ATGGGGCCAA
  961 AACAAGGATA TCTGTGGGTA AGCAGTTTCC TGCCCTCGGC TTCAAGGGCC AAGAAACAGA
 1021 TGGGTCCCCC AGAATGCGGT CCAAGCCCCT CAGCAGCTTT CTAGAGGATC AATCCAGATG
1081 TTTCCAGGGG TGCCCTAGGA ACTTGAATGA ACCCTGGTGC CTTAATTTGA AAACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]