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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBabe-hSTING(V147L_C88S_C91S) (#RDB14356)

Retroviral expression vector of human STING, V147L_C88S_C91S mutant.

Clone info. Mammalian retroviral vector for expressing human STING (mutation, V147L. dual mutation, C88S/C91S). Suppression of STING palmitoylation inhibits the gain-of-function phenotype in the SAVI–STING.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14356 pBabe-hSTING(V147L_C88S_C91S) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(V147L_C88S_C91S) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14356).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14356_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14356_A6J7a.seq check
>D03866A6_A6J7_1_pBABE5'_F01_16_ABI24.ab1
    1 GGGGGTTGGG AACCAGTGTG GTGGTACGTA GGATTCGCCG CCACCATGCC CCACTCCAGC
   61 CTGCATCCAT CCATCCCGTG TCCCAGGGGT CACGGGGCCC AGAAGGCAGC CTTGGTTCTG
  121 CTGAGTGCCT GCCTGGTGAC CCTTTGGGGG CTAGGAGAGC CACCAGAGCA CACTCTCCGG
  181 TACCTGGTGC TCCACCTAGC CTCCCTGCAG CTGGGACTGC TGTTAAACGG GGTCTGCAGC
  241 CTGGCTGAGG AGCTGCGCCA CATCCACTCC AGGTACCGGG GCAGCTACTG GAGGACTGTG
  301 CGGGCCAGCC TGGGCAGCCC CCTCCGCCGT GGGGCCCTGT TGCTGCTGTC CATCTATTTC
  361 TACTACTCCC TCCCAAATGC GGTCGGCCCG CCCTTCACTT GGATGCTTGC CCTCCTGGGC
  421 CTCTCGCAGG CACTGAACAT CCTCCTGGGC CTCAAGGGCC TGGCCCCAGC TGAGATCTCT
  481 GCACTGTGTG AAAAAGGGAA TTTCAACGTG GCCCATGGGC TGGCATGGTC ATATTACATC
  541 GGATATCTGC GGCTGATCCT GCCAGAGCTC CAGGCCCGGA TTCGAACTTA CAATCAGCAT
  601 TACAACAACC TGCTACGGGG TGCAGTGAGC CAGCGGCTGT ATATTCTCCT CCCATTGGAC
  661 TGTGGGGTGC CTGATAACCT GAGTATGGCT GACCCCAACA TTCGCTTCCT GGATAAACTG
  721 CCCCAGCAGA CCGGTGACCA TGCTGGCATC AAGGATCGGG TTTACAGCAA CAGCATCTAT
  781 GAGCTTCTGG AGAACGGGCA GCGGGCGGGC ACCTGTGTCC TGGAGTACGC CACCCCCTTG
  841 CAGACTTTGT TTGCCATGTC ACAATACAGT CAAGCTGGCT TTAGCCGGGA GGGATAGGCT
  901 TGAGCAGGCC AAACTCTTCT GCCGGACACT TGAGGACATC CTGGGCAGAT GCCCCTGAGT
  961 CTCAGAACAA CTGCCCGCCC TCATTTGCCC TACCAGGAAC CTGCAGATGA CAGCAGCTTC
 1021 TCGCTGTCCC AGGAGGGTCT CCGGCACCTG CGGGCAAGGA GGAAAAAGGG AGAGGTTACT
1081 GGTGGGGCAG CCTGAGACCT TCAGCGGGTG GCCAGTAACC CTTCAACGAA ATGGATTC
//
Primer: PuroR-rev ()
Sequence file: RDB14356_A6J7b.seq check
>D03866A6_A6J7_1_PuroR-rev_F06_06_ABI08.ab1
    1 AATGGACTTT TTGCAAAGCC TAGGCCTCCA AAAAGCCTCC TCACTACTTC TGGAATAGCT
   61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
  121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
  181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
  241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
  301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
  361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
  421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
  481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
  541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
  601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
  661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
  721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
  781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
  841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
  901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
  961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC
 1021 CCATGGGCCA CGTTGAAATT TCCCTTTTTC AACACAGTGC AGAGATCTCA GCTGGGGCCC
 1081 AGGCCCTTTG AGCCCAGAAG ATGGTCAGTG CCTGCGAGAG CTAGGAGCAG CCATTCAGTG
1141 AGGCGGGCCG ACCCGCAATT TGGGGAA
//
Primer: Amp_R ()
Sequence file: RDB14356_A6J7c.seq check
>D03866A6_A6J7_1_Amp_R_F03_18_ABI24.ab1
    1 TTCTTTTTTA AAATTAATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAG CCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
  181 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  241 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
  301 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
  361 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
  421 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGGGC GCCAGTCCTC CGATTGACTG
  481 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
  541 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
  601 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
  661 GAGGCAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
  721 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
  781 GGAACTGACG AGTTCTGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTTGGG
  841 GGGCCGTTTT TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAAATCCG ACCCCCGTCA
  901 GGATATGTGG TTCTGGTAGG AGACGAGAAA CCTTAAAACA GTTCCCGCCC TCCGTTCTGA
  961 ATTTTTTGCT TTTCGGTTTG GGAAACCGAA AGCCCGCGCG TCTTTGTCTT GCTTGCCAGC
 1021 GCTGCAGCAT CGTTTCTGGT GTTGTCTCTG TCTGAACTGT GTTTCCTTGT AATTGTCTTG
1081 AAATTAGGCA GACCTGTTAA CCACCTCCCC TTAAGTTTTG GAACTGTATA AG
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14356_A6J7d.seq check
>D03866A6_A6J7_1_SV40pro_F_V2_F05_17_ABI24.ab1
    1 GGCCAACTTT TTTGGGAGCT AGGCTTTTGC AAAAGCTTAC CATGACCGAG TACAAGCCCA
   61 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT
  121 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA
  181 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG
  241 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG
  301 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC
  361 AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA
  421 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG
  481 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA
  541 ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG
  601 GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC
  661 AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCGAT AAAATAAAAG ATTTTATTTA
  721 GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG
  781 TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA
  841 AGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT
  901 CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT
  961 CTGTGGTAAG CAGTTCCCTG CCCCGGCTCA AGGCCCAAGA ACAGATGGGT CCCCAGATGC
 1021 GGTCCAGGCC TCAGCCAGTT TTCTAGGAGA ACCAATCAGA TGGTTTCAGG ATGCCCCGAA
1081 GGACTGAATT GACCTGATGC CTTATTTGAA CA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]