RDB14355 - pBabe-hSTING(V155M)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pBabe-hSTING(V155M) (#RDB14355)

Retroviral expression vector of human STING, V155M mutant.

Clone info.	Mammalian retroviral vector for expressing human STING (mutation, V155M). The disease (SAVI) patients have a point mutation of STING (V147L, N154S or V155M). These STING variants exhibit a gain-of-function phenotype and are able to stimulate the production of an IFN beta reporter construct.
Comment	Expression was confirmed by the depositor with western blotting.
Vector backbone	pBabe-Puro (Plasmid)
Size of vector backbone	5.2 kb
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	human TMEM173 cDNA
Depositor\|Developer	Arai, Hiroyuki \| Taguchi, Tomohiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14355	pBabe-hSTING(V155M)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(V155M) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14355).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14355_A6J7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' () Sequence file: RDB14355_A6J7a.seq
>D03866A5_A6J7_1_pBABE5'_E01_13_ABI24.ab1 1 ATTGGGCCGG GAATCCAGTT GTGGTGGTAC GGTAGGAATT CGCCGCCACC ATGCCCCACT 61 CCAGCCTGCA TCCATCCATC CCGTGTCCCA GGGGTCACGG GGCCCAGAAG GCAGCCTTGG 121 TTCTGCTGAG TGCCTGCCTG GTGACCCTTT GGGGGCTAGG AGAGCCACCA GAGCACACTC 181 TCCGGTACCT GGTGCTCCAC CTAGCCTCCC TGCAGCTGGG ACTGCTGTTA AACGGGGTCT 241 GCAGCCTGGC TGAGGAGCTG CGCCACATCC ACTCCAGGTA CCGGGGCAGC TACTGGAGGA 301 CTGTGCGGGC CTGCCTGGGC TGCCCCCTCC GCCGTGGGGC CCTGTTGCTG CTGTCCATCT 361 ATTTCTACTA CTCCCTCCCA AATGCGGTCG GCCCGCCCTT CACTTGGATG CTTGCCCTCC 421 TGGGCCTCTC GCAGGCACTG AACATCCTCC TGGGCCTCAA GGGCCTGGCC CCAGCTGAGA 481 TCTCTGCAGT GTGTGAAAAA GGGAATTTCA ACATGGCCCA TGGGCTGGCA TGGTCATATT 541 ACATCGGATA TCTGCGGCTG ATCCTGCCAG AGCTCCAGGC CCGGATTCGA ACTTACAATC 601 AGCATTACAA CAACCTGCTA CGGGGTGCAG TGAGCCAGCG GCTGTATATT CTCCTCCCAT 661 TGGACTGTGG GGTGCCTGAT AACCTGAGTA TGGCTGACCC CAACATTCGC TTCCTGGATA 721 AACTGCCCCA GCAGACCGGT GACCATGCTG GGCATCAAGG ATCGGGTTTA CAGCAACAGC 781 ATCTAATGAG CTTCTGGAGA ACGGGGCAGC GGGCGGGCAC CTGTGTCCTG GAGTACGCCA 841 CCCCCCTTGC AGACTTTGTT TGCCATGTCA CAATACAGTC AAGCTGGCCT TTAGCCCGGG 901 AAGGATAAGG GCTTGAGCCA GGCCCAAAAC TCTTCTGGCC GGGACACTTT GAGGAACATT 961 CCTTGCCAGA ATGCCCCCTG AAAGTCTCAG AAAAAACTGG CCCGCCCTCC ATTTGCCCTA 1021 CCAGGGAAAC CTTGCAAGAA GGAACAGCCA GCCTTTCCTC GCCTTGTCCC CAAGAAGGGT 1081 TTCCTCCCGG CCACCTTGCC GGGCAGGGGA GGAAAAAGGA GAAGAAAGGT TTACTTGGTG 1141 TGGGGGCAAG ACCTTGGAAA GAAACCCCTT CAAGCGGGGT GTGCCCCCAG AGTATCACTC 1201 TCTCTCCAAC GAGAAGTATG TATTGTCCTC //
Primer: PuroR-rev () Sequence file: RDB14355_A6J7b.seq
>D03866A5_A6J7_1_PuroR-rev_E06_05_ABI08.ab1 1 GCTGGACTTT TTGCAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT 61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG 121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA 181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG 241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG 301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG 361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG 421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC 481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT 541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG 601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG 661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG 721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC 781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG 841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC 901 AGCCGCTGGC TCACTGCACC CCGTAGCAGT TGTTGTAATG CTGATTGTAA GTTCGAATCC 961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC 1021 CCATGGGCCC ATGTTGAAAT TTCCCTTTTT CCACACACTG CAGAGATCTC AGCTGGGGCC 1081 AGGGCCCATG AGCCCAGAGG ATGTTCAGTG CTTGCAGAGC CAAGGAGCCA AGCATCCAAG 1141 TGCAAGGCCG GGTTCCGAAC CTGCCT //
Primer: Amp_R () Sequence file: RDB14355_A6J7c.seq
>D03866A5_A6J7_1_Amp_R_E03_15_ABI24.ab1 1 ATCTTTTTTT TCATTATTAT TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 121 GTGCCACCTG CAGCCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC 181 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT 241 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC 301 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC 361 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC 421 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA 481 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG 541 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT 601 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC 661 CGGGAGGCAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG ATTGTCTAGT GTCTATGACT 721 GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT 781 GGTGGAACTG ACGAGTTCTG AACACCCGGC CGCAACCCTG GGGAGACGTC CCAGGGACTT 841 TGGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGGAGT CGATGTGGGA ATCCGACCCC 901 GTCAGGGATA TGTGGGTTTC TGGGTAGGAG ACGAGAACCT AAAAACAGTT TCCGCCCTCC 961 CGTCTGGAAT TTTTTGCTTT TCGGGTTTGG AACCGAAGCC GCGCGTCTTG TTCTGCCTGC 1021 AGCGCTTGCA GCAATCGATC TGGTGGTTGG TTCATCTGGT CTGAACTGGT GGTTTTCTGG 1081 TATTTTGGTC CTGGAAAATT AAGGGCCAGG ACATGTGTAA CCACTTCCCT TAGTATTTGG 1141 AACCCTTAGT CACTGGGAAA AGAATGTTCA GACGGATTTC GCCTTCTCGA GCA //
Primer: SV40pro_F_V2 () Sequence file: RDB14355_A6J7d.seq
>D03866A5_A6J7_1_SV40pro_F_V2_E05_14_ABI24.ab1 1 GACAACTTTT TTGGAGGCCT AGGCTTTTGC AAAAGCTTAC CATGACCGAG TACAAGCCCA 61 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT 121 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA 181 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG 241 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG 301 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC 361 AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA 421 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG 481 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA 541 ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG 601 GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC 661 AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCGAT AAAATAAAAG ATTTTATTTA 721 GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG 781 TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA 841 GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT 901 CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA AATATGGGCC CAAACAGGGA 961 TATCTGTGGT AAGCAGTTCC TGCCCCCGGG CTCAAGGGCC CAAGACAGAT GGGTCCCCAG 1021 ATGCGGTTCC AGCCTCAGCA GTTTTCTAGG AGGAACATCA GATGGTTTTC CCAGGGTGCC 1081 CCAAGGACCT GGAATGGACC TGTTGCTTAT TTTGAAACCT TAACCA //