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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBabe-hSTING(N154S) (#RDB14354)

Retroviral expression vector of human STING, N154S mutant.

Clone info. Mammalian retroviral vector for expressing human STING (mutation, N154S). The disease (SAVI) patients have a point mutation of STING (V147L, N154S or V155M). These STING variants exhibit a gain-of-function phenotype and are able to stimulate the production of an IFN beta reporter construct.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14354 pBabe-hSTING(N154S) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(N154S) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14354).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14354_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14354_A6J7a.seq check
>D03866A4_A6J7_1_pBABE5'_D07_04_ABI08.ab1
    1 GGGGTCGGAT CAGTGTGGTG GTACGTAGGA ATTCGCCGCC ACCATGCCCC ACTCCAGCCT
   61 GCATCCATCC ATCCCGTGTC CCAGGGGTCA CGGGGCCCAG AAGGCAGCCT TGGTTCTGCT
  121 GAGTGCCTGC CTGGTGACCC TTTGGGGGCT AGGAGAGCCA CCAGAGCACA CTCTCCGGTA
  181 CCTGGTGCTC CACCTAGCCT CCCTGCAGCT GGGACTGCTG TTAAACGGGG TCTGCAGCCT
  241 GGCTGAGGAG CTGCGCCACA TCCACTCCAG GTACCGGGGC AGCTACTGGA GGACTGTGCG
  301 GGCCTGCCTG GGCTGCCCCC TCCGCCGTGG GGCCCTGTTG CTGCTGTCCA TCTATTTCTA
  361 CTACTCCCTC CCAAATGCGG TCGGCCCGCC CTTCACTTGG ATGCTTGCCC TCCTGGGCCT
  421 CTCGCAGGCA CTGAACATCC TCCTGGGCCT CAAGGGCCTG GCCCCAGCTG AGATCTCTGC
  481 AGTGTGTGAA AAAGGGAATT TCAGCGTGGC CCATGGGCTG GCATGGTCAT ATTACATCGG
  541 ATATCTGCGG CTGATCCTGC CAGAGCTCCA GGCCCGGATT CGAACTTACA ATCAGCATTA
  601 CAACAACCTG CTACGGGGTG CAGTGAGCCA GCGGCTGTAT ATTCTCCTCC CATTGGACTG
  661 TGGGGTGCCT GATAACCTGA GTATGGCTGA CCCCAACATT CGCTTCCTGG ATAAACTGCC
  721 CCAGCAGACC GGTGACCATG CTGGCATCAA GGATCGGGTT TACAGCAACA GCATCTATGA
  781 GCTTCTGGAG AACGGGCAGC GGGCGGGCAC CTGTGTCCTG GAGTACGCCA CCCCCTTGCA
  841 GACTTTGTTT GCCATGTCAC AATACAGTCA AGCTGGCTTT AGCCGGGAGG ATAGGCTTGA
  901 GCAGGCCAAA CTCTTCTGCC GGACACTTGA GGACATCCTG GCAGATGCCC CTGAGTCTCA
  961 GAACAACTGC CGCCTCATTG CCCTACCAGG AACCTGCAGA TGACAGCAGC TTCTCGCTGT
 1021 CCAGGAGTCT CCGCACTTGC GCAGAGAAAA GGAGAGTACT GTGGGCAGCT GGAGACTCAG
1081 CGGTGCAGTA CCTCACGAAT GTCCAGAGCC ATGAGATCCC T
//
Primer: PuroR-rev ()
Sequence file: RDB14354_A6J7b.seq check
>D03866A4_A6J7_1_PuroR-rev_D06_04_ABI08.ab1
    1 GCATTGAGCT TTTGCAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG
   61 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG
  121 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC
  181 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
  241 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC
  301 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGGG TCGAGTCAAG
  361 AGAAATCCGT GCGGAGAGGG AGGGGCTTTT CCATTCCACT GATGAGGAGC TCAGGCTCTT
  421 GGGACATCGT GGAGGTACTG GGCACCGCTG AGGTCTTCAA GCTGCCCACA GTAACCTCTT
  481 CCTTTTCCTC CTGCCGCAGG TGCCGGAGAA CCTCCTGGGA CAGCGAGAAG CTGCTGTCAT
  541 CTGCAGGTTC CTGGTAGGCA ATGAGGCGGC AGTTGTTCTG AGACTCAGGG GCATCTGCCA
  601 GGATGTCCTC AAGTGTCCGG CAGAAGAGTT TGGCCTGCTC AAGCCTATCC TCCCGGCTAA
  661 AGCCAGCTTG ACTGTATTGT GACATGGCAA ACAAAGTCTG CAAGGGGGTG GCGTACTCCA
  721 GGACACAGGT GCCCGCCCGC TGCCCGTTCT CCAGAAGCTC ATAGATGCTG TTGCTGTAAA
  781 CCCGATCCTT GATGCCAGCA TGGTCACCGG TCTGCTGGGG CAGTTTATCC AGGAAGCGAA
  841 TGTTGGGGTC AGCCATACTC AGGTTATCAG GCACCCCACA GTCCAATGGG AGGAGAATAT
  901 ACAGCCGCTG GCTCACTGCA CCCCGTAGCA GTTGTTGTAA TGCTGATTGT AAGTTCGAAT
  961 CCCGGGCCTG GAGCTCTGGC AGGATCAGCC GCAGATATCC GATGTAATAT GACCATGCCA
 1021 GCCCATGGGC CACGCTGAAT TCCCTTTTTC ACACACTGCA GAGATCTCAG CTGGGGCAGC
 1081 CTTGAGGCCA GAGATGTTCA GTGCCTGCGA GAGCAGGAGC CAGCCATCAA GTGAAGGCGG
1141 GCGACCGCCA ATTTGGGGAA GA
//
Primer: Amp_R ()
Sequence file: RDB14354_A6J7c.seq check
>D03866A4_A6J7_1_Amp_R_D03_12_ABI24.ab1
    1 ATCTGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCAGCC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  181 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  241 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  301 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  361 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  421 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG
  481 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  541 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  601 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  661 GGCAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  721 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  781 AACTGACGAG TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG
  841 CCGTTTTTGT GGCCCGACCT GAGGAAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT
  901 ATGTGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCTCCGTCT GAATTTTTGC
  961 TTTCGGTTTG GAAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG CATCGTTCTG
 1021 TGTGTCTCTG TCTGACTGTG TTTTCTGTAT TTTGTCTGAA AAATTAGGAC AGACCTGATA
1081 CCACTCCTTA GTTGACTTAA GTCACTGGAA AAGAATGTTC GAGCCGGGAA TCGGCT
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14354_A6J7d.seq check
>D03866A4_A6J7_1_SV40pro_F_V2_D05_11_ABI24.ab1
    1 GGCAGCTTTT TTGGAGCTAG GCTTTTGCAA AAGCTTACCA TGACCGAGTA CAAGCCCACG
   61 GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC
  121 GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC
  181 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG
  241 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG
  301 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA
  361 CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC
  421 GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA
  481 GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC
  541 CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA
  601 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG
  661 CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT
  721 CTCCAGAAAA AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA
  781 ACGCCATTTT GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG
  841 TCAGGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC
  901 TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGGCCAA ACAGGATATC
  961 TGTGGGTAAG CAGTTCCCTG CCCCGGGCTC AGGCCCAAGA CAGATGGGTC CCCAGATGCG
 1021 GGTCCCAGCC CTCAGCAGTT CTAGAGGACC ATTCAGATGT TTCAGGTGCC CCAACGACTG
1081 AAATGACCTG TGCTTATTTG ACTAACCAAT TCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]