RDB14353 - pBabe-hSTING(V147L)

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pBabe-hSTING(V147L) (#RDB14353)

Retroviral expression vector of human STING, V147L mutant.

Clone info.	Mammalian retroviral vector for expressing human STING (mutation, V147L). The disease (SAVI) patients have a point mutation of STING (V147L, N154S or V155M). These STING variants exhibit a gain-of-function phenotype and are able to stimulate the production of an IFN beta reporter construct.
Comment	Expression was confirmed by the depositor with western blotting.
Vector backbone	pBabe-Puro (Plasmid)
Size of vector backbone	5.2 kb
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	human TMEM173 cDNA
Depositor\|Developer	Arai, Hiroyuki \| Taguchi, Tomohiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14353	pBabe-hSTING(V147L)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(V147L) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14353).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14353_A6J7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' () Sequence file: RDB14353_A6J7a.seq
>D03866A3_A6J7_1_pBABE5'_A07_01_ABI08.ab1 1 AGGGTCGGGA TCAGTGTGGT GGTACGTAGG AATTCGCCGC CACCATGCCC CACTCCAGCC 61 TGCATCCATC CATCCCGTGT CCCAGGGGTC ACGGGGCCCA GAAGGCAGCC TTGGTTCTGC 121 TGAGTGCCTG CCTGGTGACC CTTTGGGGGC TAGGAGAGCC ACCAGAGCAC ACTCTCCGGT 181 ACCTGGTGCT CCACCTAGCC TCCCTGCAGC TGGGACTGCT GTTAAACGGG GTCTGCAGCC 241 TGGCTGAGGA GCTGCGCCAC ATCCACTCCA GGTACCGGGG CAGCTACTGG AGGACTGTGC 301 GGGCCTGCCT GGGCTGCCCC CTCCGCCGTG GGGCCCTGTT GCTGCTGTCC ATCTATTTCT 361 ACTACTCCCT CCCAAATGCG GTCGGCCCGC CCTTCACTTG GATGCTTGCC CTCCTGGGCC 421 TCTCGCAGGC ACTGAACATC CTCCTGGGCC TCAAGGGCCT GGCCCCAGCT GAGATCTCTG 481 CACTGTGTGA AAAAGGGAAT TTCAACGTGG CCCATGGGCT GGCATGGTCA TATTACATCG 541 GATATCTGCG GCTGATCCTG CCAGAGCTCC AGGCCCGGAT TCGAACTTAC AATCAGCATT 601 ACAACAACCT GCTACGGGGT GCAGTGAGCC AGCGGCTGTA TATTCTCCTC CCATTGGACT 661 GTGGGGTGCC TGATAACCTG AGTATGGCTG ACCCCAACAT TCGCTTCCTG GATAAACTGC 721 CCCAGCAGAC CGGTGACCAT GCTGGCATCA AGGATCGGGT TTACAGCAAC AGCATCTATG 781 AGCTTCTGGA GAACGGGCAG CGGGCGGGCA CCTGTGTCCT GGAGTACGCC ACCCCCTTGC 841 AGACTTTGTT TGCCATGTCA CAATACAGTC AAGCTGGCTT TAGCCGGGAG GATAGGCTTG 901 AGCAGGCCAA ACTCTTCTGC CGGACACTTG AGGACATCCT GGCAGATGCC CCTGAGTCTC 961 AGAACAACTG CCCGCCTCAT TGCCTACCAG GAAACCTGCA GATGACAGCA GCTTCTCGCT 1021 GGTCCCAGGA GTTCTCCGGC ACTTGCGCAG AGGAAAAGGA GAGTACTGTG GGCCAGCTTT 1081 GAGACTCAGC GTGCCAGCTA CTCACGATGT TTCCAAGAGA GAGCCTGAGC T //
Primer: PuroR-rev () Sequence file: RDB14353_A6J7b.seq
>D03866A3_A6J7_1_PuroR-rev_C06_03_ABI08.ab1 1 GAATGTGCTT TTGCAAAGCC TAGGCCTCCA AAAAGCCTCC TCACTACTTC TGGAATAGCT 61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG 121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA 181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG 241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG 301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG 361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG 421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC 481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT 541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG 601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG 661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG 721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC 781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG 841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC 901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC 961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCC GCAGATATCC GATGTAATAT GACCATGCCA 1021 GCCCATGGGC CACGTTTGAA ATTCCCCTTT TTCACACAGT GCAGAGATCT CAGCTGGGGC 1081 CAGGCCCCTT GGAGACCAGA GATGTTCAGT GCTTGCAGAG CAGGAAGCAG CATCAGTGAA 1141 GGCCGGCCGA ACCGCCAAAG //
Primer: Amp_R () Sequence file: RDB14353_A6J7c.seq
>D03866A3_A6J7_1_Amp_R_C03_09_ABI24.ab1 1 TTCCCCTTTT TATAAATTTA ATTGAAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 61 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 121 AAGTGCCACC TGCAGCCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC 181 CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG 241 GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCAGC 301 CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA 361 CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT 421 GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGGGCGCCAG TCCTCCGATT 481 GACTGAGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG 541 TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT 601 CTTTCATTTG GGGGCTCGTC CGGGATCGGG AGACCCCTGC CCAGGGACCA CCGACCCACC 661 ACCGGGAGGC AAGCTGGCCA GCAACTTATC TGTGTCTGTC CGATTGTCTA GTGTCTATGA 721 CTGATTTTAT GCGCCTGCGT CGGTACTAGT TAGCTAACTA GCTCTGTATC TGGCGGACCC 781 GTGGTGGAAC TGACGAGTTC TGAACACCCG GCCGCAACCC TGGGAGACGT CCCAGGGACT 841 TTGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGAAGT CGATGTGGAA TCCGACCCCG 901 TCAGGATATG TGGGTTCTGG GTAGGGAGAC GAGAAACCTA AAACAGGTTC CCGCCTTCCG 961 TCTGAAATTT TTTGCCTTTC GGGTTTGGGA ACCCGAAGCC GCGCGTTCTT GTCTGCCTGC 1021 AACCGCTTGC AGCAATCGGT TCTGTGTTTG TCTCTTGTCC TGACTGTGTT CTGTTATTTG 1081 GTCTGGAAAA AATTAAGGGC CAGAACTTGT TAACCACCTC CCTTCAGCTT TGAACCTTAA 1141 AGTCACTGGA AAAGAATGGT CCGAGGAGCG GAATTGCGGT CT //
Primer: SV40pro_F_V2 () Sequence file: RDB14353_A6J7d.seq
>D03866A3_A6J7_1_SV40pro_F_V2_C07_03_ABI08.ab1 1 GTCCAACTTT TTTTGGGAGC CTAGGCTTTT GCAAAAGCTT ACCATGACCG AGTACAAGCC 61 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 121 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 181 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 241 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 301 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 361 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 421 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 481 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 541 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA 601 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC 661 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATAAAATAAA AGATTTTATT 721 TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA 781 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC 841 AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 901 TTCCTGCCCC GGCTCAGGCC AAGAACAGAT GGAAACAGCT GAATATGGGC CAAACAGGAT 961 ATCTGTGGTA AGCAGTTCCT GCCCCGGGCT CAGGCCAAGA AACAGGATGG GTCCCCAGAT 1021 GCGTCCCAGC CCTCAGCCAG GTTTCTAGAA GAACCATCAG GATGTTTCAG GGTGCCCCAA 1081 CGACTGAATG AACCTGTGCT ATTTGGACCT AACCAATTCC AGATTCGCT //