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pBabe-hSTING(C88/91S) (#RDB14352)

Retroviral expression vector of human STING, C88/91S mutant.

Clone info. Mammalian retroviral vector for expressing human STING (dual mutation, C88S/C91S). Cysteine residues are the site of protein palmitoylation. The double mutation of Cysteine results in loss of the palmitoylation.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14352 pBabe-hSTING(C88/91S) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(C88/91S) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14352).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14352_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14352_A6J7a.seq check
>D03866A2_A6J7_1_pBABE5'_B01_04_ABI24.ab1
    1 GTGTGTTCTG GAACAAGTGT GGTGGTACGT AGGAATTCGC CGCCACCATG CCCCACTCCA
   61 GCCTGCATCC ATCCATCCCG TGTCCCAGGG GTCACGGGGC CCAGAAGGCA GCCTTGGTTC
  121 TGCTGAGTGC CTGCCTGGTG ACCCTTTGGG GGCTAGGAGA GCCACCAGAG CACACTCTCC
  181 GGTACCTGGT GCTCCACCTA GCCTCCCTGC AGCTGGGACT GCTGTTAAAC GGGGTCTGCA
  241 GCCTGGCTGA GGAGCTGCGC CACATCCACT CCAGGTACCG GGGCAGCTAC TGGAGGACTG
  301 TGCGGGCCAG CCTGGGCAGC CCCCTCCGCC GTGGGGCCCT GTTGCTGCTG TCCATCTATT
  361 TCTACTACTC CCTCCCAAAT GCGGTCGGCC CGCCCTTCAC TTGGATGCTT GCCCTCCTGG
  421 GCCTCTCGCA GGCACTGAAC ATCCTCCTGG GCCTCAAGGG CCTGGCCCCA GCTGAGATCT
  481 CTGCAGTGTG TGAAAAAGGG AATTTCAACG TGGCCCATGG GCTGGCATGG TCATATTACA
  541 TCGGATATCT GCGGCTGATC CTGCCAGAGC TCCAGGCCCG GATTCGAACT TACAATCAGC
  601 ATTACAACAA CCTGCTACGG GGTGCAGTGA GCCAGCGGCT GTATATTCTC CTCCCATTGG
  661 ACTGTGGGGT GCCTGATAAC CTGAGTATGG CTGACCCCAA CATTCGCTTC CTGGATAAAC
  721 TGCCCCAGCA GACCGGTGAC CATGCTGGCA TCAAGGATCG GGTTTACAGC AACAGCATCT
  781 ATGAGCTTCT GGAGAACGGG CAGCGGGCGG GCACCTGTGT CCTGGAGTAC GCCACCCCCT
  841 TGCAGACTTT GTTTGCCATG TCACAAATAC AGTCAAAGCT GGCTTTAGCC GGGAGGATAG
  901 GCTTGAGCAG GCCAAAACTC TTTCTGCCGG ACACTTGAGG ACATCCCTGG CAGATGCCCC
  961 TGAGTCTCAG AACAACTGCC GGCCTTCATT TGCCTACCAG GAACCCTGCA GAATGACAGC
 1021 AGCTTTCTCG CTGGTCCCAA GAGATTCTTC CGGCACCCTG CGGCAGGAGG AAAATGAGAG
 1081 GGTTAACTGT GGGGCCAGCC TGGAGACTTC AACGTTGCCC AAGTATACCC TCCCACGGAA
1141 TGGATTCCCA
//
Primer: PuroR-rev ()
Sequence file: RDB14352_A6J7b.seq check
>D03866A2_A6J7_1_PuroR-rev_B06_02_ABI08.ab1
    1 GCTTAGACTT TTTGCAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC
   61 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC
  121 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT
  181 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
  241 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT
  301 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGGGT CGAGTCAAGA
  361 GAAATCCGTG CGGAGAGGGA GGGGCTTTTC CATTCCACTG ATGAGGAGCT CAGGCTCTTG
  421 GGACATCGTG GAGGTACTGG GCACCGCTGA GGTCTTCAAG CTGCCCACAG TAACCTCTTC
  481 CTTTTCCTCC TGCCGCAGGT GCCGGAGAAC CTCCTGGGAC AGCGAGAAGC TGCTGTCATC
  541 TGCAGGTTCC TGGTAGGCAA TGAGGCGGCA GTTGTTCTGA GACTCAGGGG CATCTGCCAG
  601 GATGTCCTCA AGTGTCCGGC AGAAGAGTTT GGCCTGCTCA AGCCTATCCT CCCGGCTAAA
  661 GCCAGCTTGA CTGTATTGTG ACATGGCAAA CAAAGTCTGC AAGGGGGTGG CGTACTCCAG
  721 GACACAGGTG CCCGCCCGCT GCCCGTTCTC CAGAAGCTCA TAGATGCTGT TGCTGTAAAC
  781 CCGATCCTTG ATGCCAGCAT GGTCACCGGT CTGCTGGGGC AGTTTATCCA GGAAGCGAAT
  841 GTTGGGGTCA GCCATACTCA GGTTATCAGG CACCCCACAG TCCAATGGGA GGAGAATATA
  901 CAGCCGCTGG CTCACTGCAC CCCGTAGCAG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
  961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCC GCAGATATCC GATGTAATAT GACCATGCCC
 1021 AGCCCATGGG CCACGTTGAA ATTCCCTTTT TTCACAACAC TGCAGAGATC TCAGCTGGGG
 1081 GCCAGGCCTT GGAGCCAGAG ATGTCAGTGA CTTGCCAGAG CCAGAGGGCA AGCATCCAAG
1141 TGACGGCGCG ACGCAATTGG CAGGGAGCTT AGAT
//
Primer: Amp_R ()
Sequence file: RDB14352_A6J7c.seq check
>D03866A2_A6J7_1_Amp_R_B03_06_ABI24.ab1
    1 CTCCCTTTTT ACATATTTAT TGAAGGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG CAGCCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
  181 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT
  241 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
  301 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC
  361 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
  421 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA
  481 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG
  541 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT
  601 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC
  661 CGGGAGGCAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG ATTGTCTAGT GTCTATGACT
  721 GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT
  781 GGTGGAACTG ACGAGTTCTG AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTT
  841 GGGGGGCCGT TTTTGTGGCC CGACCTGAGG AAGGGGAGTC GATGTGGGAA TCCGACCCCG
  901 TCAGGATATG TGGTTCTGGG TAGGAGACGA GAAACCTAAA ACAGGTTCCC GCCTCCGTCT
  961 GAATTTTTGC TTTCGGTTTG GAACCGAAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG
 1021 CATCGTTTCT GTGTTGGTCT CTGTTCTGGA CTGGTGTTTC TGGTATTTGT CTGAAAATTT
 1081 AGGCAGACTT GTTTACCACT TCCTTAGGTT GACCTAAGGT CACTGGAAAG AAATGGTTCG
1141 TAAGCCGG
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14352_A6J7d.seq check
>D03866A2_A6J7_1_SV40pro_F_V2_B05_05_ABI24.ab1
    1 GAGCGGCTTT TTTGGAGCTA GGCTTTTGCA AAAGCTTACC ATGACCGAGT ACAAGCCCAC
   61 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT
  121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC
  181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
  241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
  301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
  361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
  421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
  481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
  541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
  601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA
  661 GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG
  721 TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT
  781 AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG
  841 GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
  901 CTGCCCCGGC TCAGGGCCAA GAACAGATGG AAACAGCTGA AATATGGGCC CAAACAGGAT
  961 ATCTGTGGGT AAAGCAGTTT CCTGCCCCCG GGCTCAGGCC CAAGAACAGA TGGGTCCCCC
 1021 AGATGCGGGT CCAAGCCCTC CAGCAGTTTT CTAGAGAATC ATCAGATGGT TTCCAGGGTG
1081 CCTCAGGAAC TTGAATGAAC CTTGTTGCCG TTAATTTTGA ACCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]