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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBabe-hSTING(WT) (#RDB14351)

Retroviral expression vector of human STING.

Clone info. Mammalian retroviral vector for expressing human STING (wild type).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14351 pBabe-hSTING(WT) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(WT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14351).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14351_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14351_A6J7a.seq check
>D03866A1_A6J7_1_pBABE5'_A01_01_ABI24.ab1
    1 GTGGGTGGGG AATCCAGGTG TGGTGGTACG TAGGAATTCG CCGCCACCAT GCCCCACTCC
   61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT
  121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC
  181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC
  241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT
  301 GTGCGGGCCT GCCTGGGCTG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT
  361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG
  421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC
  481 TCTGCAGTGT GTGAAAAAGG GAATTTCAAC GTGGCCCATG GGCTGGCATG GTCATATTAC
  541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG
  601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG
  661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA ACATTCGCTT CCTGGATAAA
  721 CTGCCCCAGC AGACCGGTGA CCATGCTGGC ATCAAGGATC GGGTTTACAG CAACAGCATC
  781 TATGAGCTTC TGGAGAACGG GCAGCGGGCG GGCACCTGTG TCCTGGAGTA CGCCACCCCC
  841 TTGCAGACTT TGTTTGCCAT GTCACAATAC AGTCAAGCTG GCTTTAGCCG GGGAGGATAG
  901 GCTTGAGCAG GCCAAACTCT TCTGCCGGGA CACTTGAGGA CATCCTGGCA GATGCCCCTG
  961 AGTCTCAGAA ACAACTGCCC GCCTCATTGC CCTACCAGGG AACCTGCAGA TGGACAGCAG
 1021 CTTCTCGCTG TCCCAGAGGA TCTCCGGGCA CTGCGGCAGG AAGAAAGAGA GTTACTGGTG
1081 GGCAGCTGAG ACTTCAAGCG GTGCCAGTTA AACCCTTCCA CGGAATGGTT C
//
Primer: PuroR-rev ()
Sequence file: RDB14351_A6J7b.seq check
>D03866A1_A6J7_1_PuroR-rev_A06_01_ABI08.ab1
    1 CGTAGGCTTT TTGCAAAGCC TAGGCCTCCA AAAAGCCTCC TCACTACTTC TGGAATAGCT
   61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
  121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
  181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
  241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
  301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
  361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
  421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
  481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
  541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
  601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
  661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
  721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
  781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
  841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
  901 AGCCGCTGGC TCACTGCACC CCGTAGCAGT TGTTGTAATG CTGATTGTAA GTTCGAATCC
  961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC
 1021 CCATGGCCAC GTTGAAAATT CCCTTTTTCA ACACACTGCA GAGATCTCAG CTGGGGGCCA
 1081 GGGCCCTTTG AAGCCAGAGA ATGGTTCAGT GCCTGCCAGA GTCAGGAGGC CAGCCATCAA
1141 GTGAAGGGCG GGCCCGACCG CCAATTTGTG
//
Primer: Amp_R ()
Sequence file: RDB14351_A6J7c.seq check
>D03866A1_A6J7_1_Amp_R_A03_03_ABI24.ab1
    1 TTTTATTTTT CAAATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGCAGC CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  181 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  241 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  301 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  361 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  421 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA
  481 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  541 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  601 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  661 AGGCAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  721 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  781 GAACTGACGA GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTTGGGG
  841 GCCGTTTTTG TGGCCCGACC TGAGGAAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA
  901 TATGTGGTTC TGGTAGGAGA CGAGAACCCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT
  961 GCTTTCGGTT TGGAACCGAA AGCCGCGCGT CTTTGTCTGC TGCAGCGCTG CAGCAATCGT
 1021 TTCTGTGTTG TCTCTGTTCT GACTGTGTTT CTGTATTTGT TCTGAAAAAT TACGGTCAGA
 1081 CCTGTTACCA ACTCCTAAGT TGGAACCTTA GTCACTGGAA AGATTGTCCA GAGCGGGAAA
1141 TTGGC
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14351_A6J7d.seq check
>D03866A1_A6J7_1_SV40pro_F_V2_A05_02_ABI24.ab1
    1 GGTCCAGTTT TTTTGGGAGG CCTAGGCTTT TGCAAAAAGC TTACCATGAC CGAGTACAAG
   61 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC
  121 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG
  181 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
  241 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
  301 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
  361 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG
  421 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC
  481 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC
  541 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC
  601 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC
  661 CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT CGATAAAATA AAAGATTTTA
  721 TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT
  781 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA
  841 TCAAGGTCAG GAACAGATGG AAACAGCTGA AATATGGGGC CAAACAGGGA TATCTGTGGT
  901 AAGCAGGTTT CCTGCCCCGG CTCAGGCCCA AGAAACAGAT GGAAACAGCT GAAATATGGG
  961 GCCAAACAGG ATATCTGTGG TAAGCAGTTT CCCTGCCCCC GGCTCAAGGG CCCAAGAACA
 1021 GATGGTCCCC AGATGCGTCC AGCCCTCAGC AGTTTCTAGA GACCAATCAG ATGTTTCCAA
1081 GGGTTGCCCC AGGACTGGAA TTGAACCCTG GTGCTTAATT GAACCTTAAA CCAAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]