RDB14344 - pCAGGS/hBACE1 N153S

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pCAGGS/hBACE1 N153S (#RDB14344)

Expression vector of human BACE1mutant, N153S.

Alternative name	pCAGGS/human BACE1 Asn153Ser
Clone info.	Expression vector of human BACE1mutant, N153S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Comment	Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone	pCAGGS (plasmid)
Selectable markers	Amp^r
Gene/insert name	human BACE1 cDNA
Depositor\|Developer	Taniguchi, Naoyuki \| Kizuka, Yasuhiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14344	pCAGGS/hBACE1 N153S	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS/hBACE1 N153S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14344).

Reference section:

Kizuka, Y., Nakano, M., Kitazume, S., Saito, T., Saido, T.C., Taniguchi, N., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14344_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14344_A6Ita.seq
>D03881A2_A6It_2_pAxCA_F1_A10_01_ABI24.ab1 1 AAGGGGAATA CCCGTCCTAA CATGTTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC 61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCATGGC CCAAGCCCTG 121 CCCTGGCTCC TGCTGTGGAT GGGCGCGGGA GTGCTGCCTG CCCACGGCAC CCAGCACGGC 181 ATCCGGCTGC CCCTGCGCAG CGGCCTGGGG GGCGCCCCCC TGGGGCTGCG GCTGCCCCGG 241 GAGACCGACG AAGAGCCCGA GGAGCCCGGC CGGAGGGGCA GCTTTGTGGA GATGGTGGAC 301 AACCTGAGGG GCAAGTCGGG GCAGGGCTAC TACGTGGAGA TGACCGTGGG CAGCCCCCCG 361 CAGACGCTCA ACATCCTGGT GGATACAGGC AGCAGTAACT TTGCAGTGGG TGCTGCCCCC 421 CACCCCTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATACCG GGACCTCCGG 481 AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAAG GGGAGCTGGG CACCGACCTG 541 GTAAGCATCC CCCATGGCCC CAGCGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 601 TCAGACAAGT TCTTCATCAA CGGCTCCAAC TGGGAAGGCA TCCTGGGGCT GGCCTATGCT 661 GAGATTGCCA GGCCTGACGA CTCCCTGGAG CCTTTCTTTG ACTCTCTGGT AAAGCAGACC 721 CACGTTCCCA ACCTCTTCTC CCTGCAGCTT TGTGGTGCTG GCTTCCCCCT CAACCAGTCT 781 GAAGTGCTGG CCTCTGTCGG AGGGAGCATG ATCATTGGAG GTATCGACCA CTCGCTGTAC 841 ACAGGCAGTC TCTGGTATAC ACCCATCCGG GCGGGGAGTG GTATTATGAG GTGATCATTG 901 TGCGGGTGGA GATCAATGGA CAGGGATCTG AAAAATGGGA CTGCAAGGAG TACCAACTAT 961 GGACAAAGAG CATTTGTGGA CAGTGGGCAC CACCCAACCC TTCGTTTTGC CCAAGAAAAG 1021 TTGTTTTGAA GACCTGCAGA TCAAAATCCC ATCAAGGGCA AGCCCTCCCT TCCACTGGAA 1081 GAAAGATTCC CCTGAAATGG TTTTCCTGGG CATAAGGAAA GAGCCAGGCT TGGTGGGATG 1141 CTGGGCCAAG CCAAGCACCC AACCCCCTTG GGAAAACATA TTTTTCCCAA AGTTAATTCC 1201 TTCACACTGT CTATACCCTA TAATGTGTGG TCGTAGAGTA //
Primer: pCA-bgter Sequence file: RDB14344_A6Itb.seq
>D03881A2_A6It_2_pCA-bgter_B10_04_ABI24.ab1 1 AGGCGCCCTT AATGGGGCCA GAAATTTTTG GCAGAGGGAA AAGATCTCAG TGGTATTTGT 61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG 121 TGAATTCTCA CTTCAGCAGG GAGATGTCAT CAGCAAAGTC ATCATGCTGC TGGCGCAGGC 181 AGCGGAGGCA GCGCCACTGA CACACCATGA GGCAGAGTGG CAGCATGAAG AGGGCGCAGA 241 TGGCAGCCAT GACATAGGCT ATGGTCATGA GGGTTGACTC ATCTGTCTGT GGAATGTTGT 301 AGCCACAGTC TTCCATGTCC AAGGTGACAA AAGGGCCTTC CACCGCTGCC GTCCTGAACT 361 CATCGTGCAC ATGGCAAGCG CTGACAGCAA AGCCAATTCG TTTTCGGGCC CGATCAAAGA 421 CAACGTAGAA GCCCTCCATG ATAACAGCTC CCATAACAGT GCCCGTGGAT GACTGTGAGA 481 TGGCAAACTT GTAACAGTCG TCTTGGGACG TGGCCACATC TTCCACTGGC CGCAGGTATT 541 GCTGCGGAAG GATGGTGATG CGGAAGGACT GGTTGGTAAC CTCACCCATT AGGTAGAGTG 601 AGATGACTGG GAAAATGTTC CAAGGGGTGG TGCCTGCTTG CCAGCACACC AGCTGCTCTC 661 CTAGCCAGAA ACCATCAGGG AACTTCTCCG TGGAGGAGGC TGCCTTGATG GATTTGACTG 721 CAGCTTCAAA ACACTTTCTT GGGCAAACGA AGGTTGGTGG TGCCACTGTC CACAATGCTC 781 TTGTCATAGT TGTACTCCTT GCAGTCCATT TTCAGATCCT GTCCATTGAT CTCCACCCGC 841 ACAATGATCA CCTCATAATA CCACTCCCGC CGGATGGGTG TATACCAGAG ACTGCCTGTG 901 TACAGCGAGT GGTCGATACC TCCAATGATC ATGCTCCCTC CGACAGAGGC CAGCACTTCA 961 GACTGGTTGA GGGGGGAAGC CAGCACCACA AGCTGCAGGG AGAAGAGGTT GGGAACGTGG 1021 GTCTGCTTTA CCAGAGAGTC AAAGAAAGGC TCCAGGGAGT CGTCAAGGCC TGGCAATTCT 1081 CAGCATAGCC AGTCCCAGGA TGCCCTTTCC CAGATGGGAG CCGTGAATGA GACTTGTCTG 1141 GATCAATGAA TGGCAGCAAT GTTTGGCAAC GCAACAGAGG TGATACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

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human BACE1

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human BACE1

Reference sequence