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Expression vector of human BACE1mutant, N153S.
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External Database human BACE1 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016) |
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| Additional terms and conditions for distribution | Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
| Ordering | Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。 |
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| 付加的提供条件 | CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
| 提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ] |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB14344 | pCAGGS/hBACE1 N153S | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
| original | Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB14344_A6Itp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pAxCA_F1 Sequence file: RDB14344_A6Ita.seq
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>D03881A2_A6It_2_pAxCA_F1_A10_01_ABI24.ab1 1 AAGGGGAATA CCCGTCCTAA CATGTTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC 61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCATGGC CCAAGCCCTG 121 CCCTGGCTCC TGCTGTGGAT GGGCGCGGGA GTGCTGCCTG CCCACGGCAC CCAGCACGGC 181 ATCCGGCTGC CCCTGCGCAG CGGCCTGGGG GGCGCCCCCC TGGGGCTGCG GCTGCCCCGG 241 GAGACCGACG AAGAGCCCGA GGAGCCCGGC CGGAGGGGCA GCTTTGTGGA GATGGTGGAC 301 AACCTGAGGG GCAAGTCGGG GCAGGGCTAC TACGTGGAGA TGACCGTGGG CAGCCCCCCG 361 CAGACGCTCA ACATCCTGGT GGATACAGGC AGCAGTAACT TTGCAGTGGG TGCTGCCCCC 421 CACCCCTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATACCG GGACCTCCGG 481 AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAAG GGGAGCTGGG CACCGACCTG 541 GTAAGCATCC CCCATGGCCC CAGCGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 601 TCAGACAAGT TCTTCATCAA CGGCTCCAAC TGGGAAGGCA TCCTGGGGCT GGCCTATGCT 661 GAGATTGCCA GGCCTGACGA CTCCCTGGAG CCTTTCTTTG ACTCTCTGGT AAAGCAGACC 721 CACGTTCCCA ACCTCTTCTC CCTGCAGCTT TGTGGTGCTG GCTTCCCCCT CAACCAGTCT 781 GAAGTGCTGG CCTCTGTCGG AGGGAGCATG ATCATTGGAG GTATCGACCA CTCGCTGTAC 841 ACAGGCAGTC TCTGGTATAC ACCCATCCGG GCGGGGAGTG GTATTATGAG GTGATCATTG 901 TGCGGGTGGA GATCAATGGA CAGGGATCTG AAAAATGGGA CTGCAAGGAG TACCAACTAT 961 GGACAAAGAG CATTTGTGGA CAGTGGGCAC CACCCAACCC TTCGTTTTGC CCAAGAAAAG 1021 TTGTTTTGAA GACCTGCAGA TCAAAATCCC ATCAAGGGCA AGCCCTCCCT TCCACTGGAA 1081 GAAAGATTCC CCTGAAATGG TTTTCCTGGG CATAAGGAAA GAGCCAGGCT TGGTGGGATG 1141 CTGGGCCAAG CCAAGCACCC AACCCCCTTG GGAAAACATA TTTTTCCCAA AGTTAATTCC 1201 TTCACACTGT CTATACCCTA TAATGTGTGG TCGTAGAGTA // |
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Primer: pCA-bgter Sequence file: RDB14344_A6Itb.seq
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>D03881A2_A6It_2_pCA-bgter_B10_04_ABI24.ab1 1 AGGCGCCCTT AATGGGGCCA GAAATTTTTG GCAGAGGGAA AAGATCTCAG TGGTATTTGT 61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG 121 TGAATTCTCA CTTCAGCAGG GAGATGTCAT CAGCAAAGTC ATCATGCTGC TGGCGCAGGC 181 AGCGGAGGCA GCGCCACTGA CACACCATGA GGCAGAGTGG CAGCATGAAG AGGGCGCAGA 241 TGGCAGCCAT GACATAGGCT ATGGTCATGA GGGTTGACTC ATCTGTCTGT GGAATGTTGT 301 AGCCACAGTC TTCCATGTCC AAGGTGACAA AAGGGCCTTC CACCGCTGCC GTCCTGAACT 361 CATCGTGCAC ATGGCAAGCG CTGACAGCAA AGCCAATTCG TTTTCGGGCC CGATCAAAGA 421 CAACGTAGAA GCCCTCCATG ATAACAGCTC CCATAACAGT GCCCGTGGAT GACTGTGAGA 481 TGGCAAACTT GTAACAGTCG TCTTGGGACG TGGCCACATC TTCCACTGGC CGCAGGTATT 541 GCTGCGGAAG GATGGTGATG CGGAAGGACT GGTTGGTAAC CTCACCCATT AGGTAGAGTG 601 AGATGACTGG GAAAATGTTC CAAGGGGTGG TGCCTGCTTG CCAGCACACC AGCTGCTCTC 661 CTAGCCAGAA ACCATCAGGG AACTTCTCCG TGGAGGAGGC TGCCTTGATG GATTTGACTG 721 CAGCTTCAAA ACACTTTCTT GGGCAAACGA AGGTTGGTGG TGCCACTGTC CACAATGCTC 781 TTGTCATAGT TGTACTCCTT GCAGTCCATT TTCAGATCCT GTCCATTGAT CTCCACCCGC 841 ACAATGATCA CCTCATAATA CCACTCCCGC CGGATGGGTG TATACCAGAG ACTGCCTGTG 901 TACAGCGAGT GGTCGATACC TCCAATGATC ATGCTCCCTC CGACAGAGGC CAGCACTTCA 961 GACTGGTTGA GGGGGGAAGC CAGCACCACA AGCTGCAGGG AGAAGAGGTT GGGAACGTGG 1021 GTCTGCTTTA CCAGAGAGTC AAAGAAAGGC TCCAGGGAGT CGTCAAGGCC TGGCAATTCT 1081 CAGCATAGCC AGTCCCAGGA TGCCCTTTCC CAGATGGGAG CCGTGAATGA GACTTGTCTG 1141 GATCAATGAA TGGCAGCAAT GTTTGGCAAC GCAACAGAGG TGATACG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. |
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2022.05.17
GNP_filter3_RDBDEP_html_220516.pl