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pCAGGS/hBACE1 N153S

Expression vector of human BACE1mutant, N153S.

Catalog number RDB14344
Resource name pCAGGS/hBACE1 N153S
Alternative name pCAGGS/human BACE1 Asn153Ser
Clone info. Expression vector of human BACE1mutant, N153S. This Asn amino acid is N-glycosylation site of BACE1, of which there are four potential sites.
Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 
Other clones in our bank human BACE1 (NCBI Gene 23621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
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RDB14344 pCAGGS/hBACE1 N153S DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14344_A6Itp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14344_A6Ita.seq
>D03881A2_A6It_2_pAxCA_F1_A10_01_ABI24.ab1
    1 AAGGGGAATA CCCGTCCTAA CATGTTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
   61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCATGGC CCAAGCCCTG
  121 CCCTGGCTCC TGCTGTGGAT GGGCGCGGGA GTGCTGCCTG CCCACGGCAC CCAGCACGGC
  181 ATCCGGCTGC CCCTGCGCAG CGGCCTGGGG GGCGCCCCCC TGGGGCTGCG GCTGCCCCGG
  241 GAGACCGACG AAGAGCCCGA GGAGCCCGGC CGGAGGGGCA GCTTTGTGGA GATGGTGGAC
  301 AACCTGAGGG GCAAGTCGGG GCAGGGCTAC TACGTGGAGA TGACCGTGGG CAGCCCCCCG
  361 CAGACGCTCA ACATCCTGGT GGATACAGGC AGCAGTAACT TTGCAGTGGG TGCTGCCCCC
  421 CACCCCTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATACCG GGACCTCCGG
  481 AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAAG GGGAGCTGGG CACCGACCTG
  541 GTAAGCATCC CCCATGGCCC CAGCGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA
  601 TCAGACAAGT TCTTCATCAA CGGCTCCAAC TGGGAAGGCA TCCTGGGGCT GGCCTATGCT
  661 GAGATTGCCA GGCCTGACGA CTCCCTGGAG CCTTTCTTTG ACTCTCTGGT AAAGCAGACC
  721 CACGTTCCCA ACCTCTTCTC CCTGCAGCTT TGTGGTGCTG GCTTCCCCCT CAACCAGTCT
  781 GAAGTGCTGG CCTCTGTCGG AGGGAGCATG ATCATTGGAG GTATCGACCA CTCGCTGTAC
  841 ACAGGCAGTC TCTGGTATAC ACCCATCCGG GCGGGGAGTG GTATTATGAG GTGATCATTG
  901 TGCGGGTGGA GATCAATGGA CAGGGATCTG AAAAATGGGA CTGCAAGGAG TACCAACTAT
  961 GGACAAAGAG CATTTGTGGA CAGTGGGCAC CACCCAACCC TTCGTTTTGC CCAAGAAAAG
 1021 TTGTTTTGAA GACCTGCAGA TCAAAATCCC ATCAAGGGCA AGCCCTCCCT TCCACTGGAA
 1081 GAAAGATTCC CCTGAAATGG TTTTCCTGGG CATAAGGAAA GAGCCAGGCT TGGTGGGATG
 1141 CTGGGCCAAG CCAAGCACCC AACCCCCTTG GGAAAACATA TTTTTCCCAA AGTTAATTCC
1201 TTCACACTGT CTATACCCTA TAATGTGTGG TCGTAGAGTA
//
Primer: pCA-bgter RDB14344_A6Itb.seq
>D03881A2_A6It_2_pCA-bgter_B10_04_ABI24.ab1
    1 AGGCGCCCTT AATGGGGCCA GAAATTTTTG GCAGAGGGAA AAGATCTCAG TGGTATTTGT
   61 GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG
  121 TGAATTCTCA CTTCAGCAGG GAGATGTCAT CAGCAAAGTC ATCATGCTGC TGGCGCAGGC
  181 AGCGGAGGCA GCGCCACTGA CACACCATGA GGCAGAGTGG CAGCATGAAG AGGGCGCAGA
  241 TGGCAGCCAT GACATAGGCT ATGGTCATGA GGGTTGACTC ATCTGTCTGT GGAATGTTGT
  301 AGCCACAGTC TTCCATGTCC AAGGTGACAA AAGGGCCTTC CACCGCTGCC GTCCTGAACT
  361 CATCGTGCAC ATGGCAAGCG CTGACAGCAA AGCCAATTCG TTTTCGGGCC CGATCAAAGA
  421 CAACGTAGAA GCCCTCCATG ATAACAGCTC CCATAACAGT GCCCGTGGAT GACTGTGAGA
  481 TGGCAAACTT GTAACAGTCG TCTTGGGACG TGGCCACATC TTCCACTGGC CGCAGGTATT
  541 GCTGCGGAAG GATGGTGATG CGGAAGGACT GGTTGGTAAC CTCACCCATT AGGTAGAGTG
  601 AGATGACTGG GAAAATGTTC CAAGGGGTGG TGCCTGCTTG CCAGCACACC AGCTGCTCTC
  661 CTAGCCAGAA ACCATCAGGG AACTTCTCCG TGGAGGAGGC TGCCTTGATG GATTTGACTG
  721 CAGCTTCAAA ACACTTTCTT GGGCAAACGA AGGTTGGTGG TGCCACTGTC CACAATGCTC
  781 TTGTCATAGT TGTACTCCTT GCAGTCCATT TTCAGATCCT GTCCATTGAT CTCCACCCGC
  841 ACAATGATCA CCTCATAATA CCACTCCCGC CGGATGGGTG TATACCAGAG ACTGCCTGTG
  901 TACAGCGAGT GGTCGATACC TCCAATGATC ATGCTCCCTC CGACAGAGGC CAGCACTTCA
  961 GACTGGTTGA GGGGGGAAGC CAGCACCACA AGCTGCAGGG AGAAGAGGTT GGGAACGTGG
 1021 GTCTGCTTTA CCAGAGAGTC AAAGAAAGGC TCCAGGGAGT CGTCAAGGCC TGGCAATTCT
 1081 CAGCATAGCC AGTCCCAGGA TGCCCTTTCC CAGATGGGAG CCGTGAATGA GACTTGTCTG
1141 GATCAATGAA TGGCAGCAAT GTTTGGCAAC GCAACAGAGG TGATACG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

2018.12.11

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