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pcDNA6/hBACE1 deltaTM-myc-His (#RDB14343)

Expression vector of C-terminal Myc and 6xHis tagged human BACE1 (soluble BACE1).

Alternative name pcDNA6-myc-His A/human BACE1
Clone info. Expression vector of C-terminal Myc and 6xHis tagged human BACE1 (soluble BACE1). This clone is constructed to express only ectodomain of the BACE1 501AA isoform ranging from Met (1AA) to Ser (453AA).
Comment Expression was confirmed by the depositor with western blotting and coomassie brilliant blue (CBB) staining.
Vector backbone pcDNA6/myc-His A (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r; Blastcidin^r
Gene/insert name human BACE1 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14343 pcDNA6/hBACE1 deltaTM-myc-His DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA6/hBACE1 deltaTM-myc-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14343).

Reference section:

Kizuka, Y., Nakano, M., Kitazume, S., Saito, T., Saido, T.C., Taniguchi, N., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14343_A6Itp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14343_A6Ita.seq check
>D03881A1_A6It_1_CMV-Forward_A11_01_ABI08.ab1
    1 CCGAACACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT GGTACCGAGC TCGGATCCAC
  181 TAGTCCAGTG TGGTGGAATT CTGCAGATGC CACCATGGCC CAAGCCCTGC CCTGGCTCCT
  241 GCTGTGGATG GGCGCGGGAG TGCTGCCTGC CCACGGCACC CAGCACGGCA TCCGGCTGCC
  301 CCTGCGCAGC GGCCTGGGGG GCGCCCCCCT GGGGCTGCGG CTGCCCCGGG AGACCGACGA
  361 AGAGCCCGAG GAGCCCGGCC GGAGGGGCAG CTTTGTGGAG ATGGTGGACA ACCTGAGGGG
  421 CAAGTCGGGG CAGGGCTACT ACGTGGAGAT GACCGTGGGC AGCCCCCCGC AGACGCTCAA
  481 CATCCTGGTG GATACAGGCA GCAGTAACTT TGCAGTGGGT GCTGCCCCCC ACCCCTTCCT
  541 GCATCGCTAC TACCAGAGGC AGCTGTCCAG CACATACCGG GACCTCCGGA AGGGTGTGTA
  601 TGTGCCCTAC ACCCAGGGCA AGTGGGAAGG GGAGCTGGGC ACCGACCTGG TAAGCATCCC
  661 CCATGGCCCC AACGTCACTG TGCGTGCCAA CATTGCTGCC ATCACTGAAT CAGACAAGTT
  721 CTTCATCAAC GGCTCCAACT GGGAAGGCAT CCTGGGGCTG GCCTATGCTG AGATTGCCAG
  781 GCCTGACGAC TCCCTGGAGC CTTTCTTTGA CTCTCTGGTA AAGCAGACCC ACGTTCCCAA
  841 CCTCTTCTCC CTGCAGCTTT GTGGTGCTGG CTTCCCCCTC AACCAGTCTG AAGTGCTGGC
  901 CTCTGTCGGA GGGAGCATGA TCATTGGAGG TATCGACCAC TCGCTGTACA CAGGCAGTCT
  961 CTGGTATACA CCCATCCGGG CGGGAGTGGT ATTATGAGGT GATCATTTGT GCGGTGGGAG
 1021 ATCAATGGAC AGGATCTGAA AATGGACTGC AGAGTACACT ATGACAGGAG CAATGTGGAC
 1081 AGTGGCAACC AACCACTTTC GGTTTGGCTC AGAAAGTGTT TGAAGCTGCA GTTCCAATTC
1141 ATCAGCAGTC CTCATCCGGG GGA
//
Primer: BGH_rev2
Sequence file: RDB14343_A6Itb.seq check
>D03881A1_A6It_1_BGH_rev2_B11_02_ABI08.ab1
    1 CCATTCAGGG GATAGGAGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC TCAATGGTGA TGGTGATGAT GACCGGTATG
  121 CATATTCAGA TCCTCTTCTG AGATGAGTTT TTGTTCGAAG GGCCCTCTAG ACTCGAGTGA
  181 CTCATCTGTC TGTGGAATGT TGTAGCCACA GTCTTCCATG TCCAAGGTGA CAAAAGGGCC
  241 TTCCACCGCT GCCGTCCTGA ACTCATCGTG CACATGGCAA GCGCTGACAG CAAAGCCAAT
  301 TCGTTTTCGG GCCCGATCAA AGACAACGTA GAAGCCCTCC ATGATAACAG CTCCCATAAC
  361 AGTGCCCGTG GATGACTGTG AGATGGCAAA CTTGTAACAG TCGTCTTGGG ACGTGGCCAC
  421 ATCTTCCACT GGCCGCAGGT ATTGCTGCGG AAGGATGGTG ATGCGGAAGG ACTGGTTGGT
  481 AACCTCACCC ATTAGGTAGA GTGAGATGAC TGGGAAAATG TTCCAAGGGG TGGTGCCTGC
  541 TTGCCAGCAC ACCAGCTGCT CTCCTAGCCA GAAACCATCA GGGAACTTCT CCGTGGAGGA
  601 GGCTGCCTTG ATGGATTTGA CTGCAGCTTC AAACACTTTC TTGGGCAAAC GAAGGTTGGT
  661 GGTGCCACTG TCCACAATGC TCTTGTCATA GTTGTACTCC TTGCAGTCCA TTTTCAGATC
  721 CTGTCCATTG ATCTCCACCC GCACAATGAT CACCTCATAA TACCACTCCC GCCGGATGGG
  781 TGTATACCAG AGACTGCCTG TGTACAGCGA GTGGTCGATA CCTCCAATGA TCATGCTCCC
  841 TCCGACAGAG GCCAGCACTT CAGACTGGTT GAGGGGGAAG CCAGCACCAC AAAGCTGCAG
  901 GGAGAAGAGG TTGGGAACGT GGGTCTGCTT TACCAGAGAG TCAAAGAAAG GCTCCAGGGA
  961 GTCGTCAGGC CTGGCAATCT CAGCATAGGC CAGCCCAGGA TGCCTTCCCA GTTGGAGCCG
 1021 TTGATGAAAG AACTTGTCTG ATTCAGTGAT GGCAGCAATG TGGCACGCAC AGTGACGTTG
 1081 GGGGTCATGG GGGGATGCTA ACCAGTCGTC CTAGCTCCCA TCCAACTGGC CCTGGGTGTA
1141 GGCCACAATA CACACCCCTT CCGGA
//
Primer: SV40pro_F
Sequence file: RDB14343_A6Itc.seq check
>D03881A1_A6It-1_SV40pro_F_E12_05_ABI08.ab1
    1 GCCTTGTCCT GGGGCAATTC AGAAGTAGTG AGGAGGCTTT TTTGGAAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
  121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
  181 GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA
  241 CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG
  301 CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT
  361 GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA
  421 TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA
  481 TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG
  541 TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAAGCA CTTCGTGGCC GAGGAGCAGG
  601 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  661 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT
  721 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
  781 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC
  841 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT
  901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
  961 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
 1021 ATTGCGTTGC GCTCACTGCC CCGCTTTCCC AGTCGGGAAC CCTGTCGTGC CAGCTGCATT
 1081 AAATGAATCG GGCACGGCGC GGGAGAGGCG GGTTGCGTAA TGGGCGCCTC TCCGCATCCT
1141 CGCCTCACTG AACTCGGCGT GGCGCGCTCC TCCGGGATTC GCGATTTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158. [link to RRC of NBRP]