Resource data sheet
Prev.

pEF-T7-Varp

Expression vector of mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa).

Catalog number RDB14334
Resource name pEF-T7-Varp
Clone info. Expression vector driven by the EF1 promoter for T7-tagged mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa), see Fig. 3A of Tamura, K. et al. Mol. Biol. Cell., 20 (12): 2900-2908, 2009.
Refer GenBank, accession AB455262 for mouse Varp sequence. Expression was confirmed by the depositor with immunoprecipitation and microscopic observation.
Vector backbone pEF-T7 (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse Ankrd27 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Ankrd27 (NCBI Gene 245886) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tamura. K. et al., Mol. Biol. Cell, 20 (12): 2900-2908, 2009); (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14334 pEF-T7-Varp Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.
original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.
original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl