Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-Varp

Expression vector of FLAG-tagged mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa)

Catalog number RDB14333
Resource name pEF-FLAG-Varp
Clone info. Expression vector driven by the EF1 promoter for FLAG-tagged mouse VPS9-ankyrin-repeat protein (Varp) full-length (1-1048 aa), see Fig. 3A of Tamura, K. et al. Mol. Biol. Cell., 20 (12): 2900-2908, 2009.
Comment Refer GenBank, accession AB455262 for mouse Varp sequence. Expression was confirmed by the depositor with immunoprecipitation and microscopic observation.
Vector backbone pEF-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse Ankrd27 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Ankrd27

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tamura. K. et al., Mol. Biol. Cell, 20 (12): 2900-2908, 2009); (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tamura. K. et al., Mol. Biol. Cell, 20 (12): 2900-2908, 2009); (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB14333 pEF-FLAG-Varp Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.
          original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.
          original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl