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pIRM21-3HA-N-hTIPARP (#RDB14324)

Expression vector of HA tagged human TIPARP, deletion mutant of C3H1-type zinc finger, WWE domain and catalytic domain (237-657 AA).

Clone info. Expression vector driven by the CAG promoter for HA tagged human TCDD inducible poly (ADP-ribose) polymerase (TIPARP) mutant; deletion of C3H1-type zinc finger, WWE domain and catalytic domain (237-657 AA). See Fig. S4e of Yamada, T., Nat. Immunol, 17 (6): 687-694, 2016 for details.
Comment Expression was confirmed by the deposotor with immunoprecipitation and immunoblotting.
Vector backbone pIRM-3HA (Plasmid)
Selectable markers Amp^r
Gene/insert name human TIPARP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks Please contact us. We will provide you with appropriate documents.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
MTAに書く付加的使用条件：
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14324 pIRM21-3HA-N-hTIPARP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pIRM21-3HA-N-hTIPARP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14324).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14324_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14324_A6I5a.seq check
>D03656A2_A6I5_1_pAxCA_F1_E02_14_ABI24.ab1
    1 GTTAGCATCT GCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGCC ATGGCATACC CTTATGACGT
  121 ACCAGATTAC GCGGGATATC CCTACGATGT CCCCGATTAC GCGCGTTATC CATATGACGT
  181 CCCGGACTAT GCCGAATTCG TGATTCTCGA CATGGAAATG GAAACCACCG AACCTGAGCC
  241 AGACTGTGTA GTGCAGCCTC CCTCTCCTCC TGATGACTTT TCATGCCAAA TGAGACTCTC
  301 TGAGAAGATC ACTCCATTGA AGACTTGTTT TAAGAAAAAG GATCAGAAAA GATTGGGAAC
  361 TGGAACCCTG AGGTCTTTGA GGCCAATATT AAACACTCTT CTAGAATCTG GCTCACTTGA
  421 TGGGGTTTTT AGATCTAGGA ACCAGAGTAC AGATGAGAAC AGCTTACATG AACCTATGAT
  481 GAAGAAAGCC ATGGAAATCA ATTCATCATG CCCACCAGCA GAAAATAATA TGTCTGTTCT
  541 GATTCCTGAT AGGACAAATG TTGGGGACCA GATACCGGAA GCCCATCCTT CCACTGAAGC
  601 TCCAGAACGA GTGGTTCCAA TCCAAGATCA CAGCTTTCCA TCAGAAACCC TCAGTGGGAC
  661 GGTGGCAGAT TCCACACCAG CTCACTTCCA GACTGATCTT TTGCACCCAG TTTCAAGTGA
  721 TGTTCCTACT AGTCCTGACT GCTTAGACAA AGTCATAGAT TATGTTCCAG GCATTTTCCA
  781 AGAAAACAGT TTTACAATCC AATACATTCT GGGACACCAG TGATAAGCTG AGTACTGAGC
  841 TCTTTCAGAC AAAAGTGAAG AGGCTTCCTG ACTCGTGTTG GAGCTGGTGA CCAGTTGCAG
  901 TACACACTCA CATGGAGCGG CGCATCGTGA CTGACTGACG ATCCGGGCCG CTACGTTAAT
  961 CCGCCCCCCC TAACGGTACT GCCGAGCGCT TGGAATAGCG GTGCTTGCCA CGTTAATTCA
1021 CATTGCGTCT GGCATGAGCC GACTGCGTTC TTGCCAGACT CAGGACTTAT CCTTCGA
//
Primer: IRES-R
Sequence file: RDB14324_A6I5b.seq check
>D03656A2_A6I5_1_IRES-R_F02_17_ABI24.ab1
    1 GTCTTGCCGG GCCTCGGCCA GTAACGTTAG GGGGGGGCGG AATTTACGTA GCGGCCCGAT
   61 CGTCAGTCAG TCACGATGCG GCCGCTCATT GGTGAGTGTG GTACTGCAAC TGGTTCACCA
  121 GCTCAAACAC GAGGTCAAGG GAAGCCTCTT CACTTTTGTC CTGAAAGAGC TCAGTACTCA
  181 GCTTATCACT GGTGTCCAGA ATGTATTGGA TTGTAAAACT GTTTTCTTGG AAAATGCCTG
  241 GAACATAATC TATGACTTTG TCTAAGCAGT CAGGACTAGT AGGAACATCA CTTGAAACTG
  301 GGTGCAAAAG ATCAGTCTGG AAGTGAGCTG GTGTGGAATC TGCCACCGTC CCACTGAGGG
  361 TTTCTGATGG AAAGCTGTGA TCTTGGATTG GAACCACTCG TTCTGGAGCT TCAGTGGAAG
  421 GATGGGCTTC CGGTATCTGG TCCCCAACAT TTGTCCTATC AGGAATCAGA ACAGACATAT
  481 TATTTTCTGC TGGTGGGCAT GATGAATTGA TTTCCATGGC TTTCTTCATC ATAGGTTCAT
  541 GTAAGCTGTT CTCATCTGTA CTCTGGTTCC TAGATCTAAA AACCCCATCA AGTGAGCCAG
  601 ATTCTAGAAG AGTGTTTAAT ATTGGCCTCA AAGACCTCAG GGTTCCAGTT CCCAATCTTT
  661 TCTGATCCTT TTTCTTAAAA CAAGTCTTCA ATGGAGTGAT CTTCTCAGAG AGTCTCATTT
  721 GGCATGAAAA GTCATCAGGA GGAGAGGGAG GCTGCACTAC ACAGTCTGGC TCAGGTTCGG
  781 TGGTTTCCAT TTCCATGTCG AGAATCACGA ATTCGGCATA GTCCGGGACG TCATATGGAT
  841 AACGCGCGTA ATCGGGGACA TCGTAGGGAT ATCCCGCGTA ATCTGGTACG TCATAAGGGT
  901 ATGCCATGGC AATTCTTTGC CAAAATGATG AGACAGCACA ATAACCAGCA CGTTTGCCCA
  961 GGAGCTGTAG GAAAAAGAAG AAGCATGAAC ATGGGTTAGC AGAGGCTCTA GAGCCGCCGG
 1021 GTCACACGCC AGAAGCCGGA CCCCGCCCTG CCCCCGTCCC CCCTCGAAAG CAAGCCGTCC
1081 CCCCCCGCGG ACAGTCCCGA AGCCTTGGAA GATGGAGAAA GGGTACGGTC GGCGCCCGG
//
Primer: IRES-F
Sequence file: RDB14324_A6I5c.seq check
>D03656A2_A6I5_1_IRES-F_G02_20_ABI24.ab1
    1 GCATCTTCTG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
   61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGAGCTGT AGCAAGAACG
  121 TGATCAAGGA GTTCATGCGC TTCAAGGTGC GCATGGAGGG CACCGTGAAC GGCCACGAGT
  181 TCGAGATCAA GGGCGAGGGC GAAGGCCGCC CCTACGAGGG CCACTGTAGC GTGAAGCTGA
  241 TGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTTCGACAT CCTGAGCCCC CAGTTCCAGT
  301 ACGGCAGCAA GGTGTACGTG AAGCACCCCG CCGACATCCC CGACTACCTG AAGCTGAGCT
  361 TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG
  421 TGAGCCAGGA TTCGAGCCTG AAGGACGGCT GCTTCATCTA CGAGGTGAAG TTCATCGGCG
  481 TGAACTTCCC CAGCGACGGC CCCGTGATGC AGCGCCGCAC CCGCGGCTGG GAGGCCAGCA
  541 GCGAGCGCCT GTACCCCCGC GACGGCGTGC TGAAGGGCGA CATCCACATG GCCCTGCGCC
  601 TGGAGGGCGG CGGCCACTAC CTGGTGGAGT TCAAGAGCAT CTACATGGTG AAGAAGCCCA
  661 GCGTGCAACT GCCCGGCTAC TACTACGTGG ACAGCAAGCT GGACATGACC AGCCACAACG
  721 AGGACTACAC CGTGGTGGAG CAGTACGAGA AGACCCAGGG CCGCCTCCAC CCCTTCATCA
  781 AGCACCTACA GTGAGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC
  841 TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA
  901 ATTTTTTGTG TCTCTCACTC GGAAAGGACA TATGGGAGGG CAAATCATTT AAAACATCAG
  961 AATGAGTATT TGGGTTTAGA GTTTGGGCAA CATATGCCCA TATGCTGGGC TGCCATGACA
 1021 AAGGTTGGGC TATAAGAGTC ATCAGTATAT GAAACAGCCC CCTGCCTGGT CATCGTATCC
1081 ATAGAAGCAT GGACCTGAAG TAGATTTTTT TTAATATTTT TGGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]