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pEGFP-C1-mouse Rab35(S5A)

Expression vector of EGFP-tagged mouse Rab35/Rab5A swapping mutation.

Catalog number RDB14199
Resource name pEGFP-C1-mouse Rab35(S5A)
Clone info. Expression vector of EGFP-tagged mouse Rab35 mutant; a swapping mutation in the switch II region between Rab35 (70–76) and Rab5A (82–88), see Fig. 2A of Etoh, K., J. Biol. Chem. 290 (14): 9064-9074 (2015) for details.
Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab35 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab35 (NCBI Gene 77407) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14199 pEGFP-C1-mouse Rab35(S5A) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14199_A6Fdp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C RDB14199_A6Fda.seq
>D03515A3_A6Fd_1_EGFP-C_B05_05.ab1
    1 CAGTATCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGGCC CGGGACTACG
   61 ACCACCTCTT CAAGCTGCTC ATCATCGGCG ACAGCGGTGT GGGCAAGAGC AGCTTGCTGT
  121 TACGATTCGC AGACAACACC TTCTCAGGCA GCTACATCAC CACAATCGGA GTGGATTTCA
  181 AGATTCGGAC TGTGGAGATC AACGGGGAGA AGGTGAAGCT GCAGATCTGG GACACGGCAG
  241 GGCAGGAGCG CTACCACTCC CTCGCCCCTA TGTATTATCG GGGGACCCAT GGGGTCATTG
  301 TGGTTTACGA CGTCACTAGT GCCGAGTCCT TTGTCAACGT CAAGCGATGG CTTCATGAAA
  361 TCAACCAGAA CTGTGACGAT GTGTGCCGAA TATTAGTGGG CAATAAGAAT GATGACCCTG
  421 AGCGGAAGGT GGTAGAGACA GAAGATGCCT ACAAATTTGC CGGGCAGATG GGGATCCAGC
  481 TCTTTGAGAC CAGTGCCAAG GAGAACGTCA ATGTGGAAGA GATGTTCAAC TGTATCACAG
  541 AGCTGGTTCT GCGAGCAAAG AAAGACAACT TGGCGAAACA GCAGCAGCAA CAACAGAACG
  601 ATGTGGTGAA GCTCACCAAA AACAGTAAAC GAAAGAAACG CTGCTGCTAA GTCGACGGTA
  661 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG
  721 TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA
  781 TGAATGCAAT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  841 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTTCA CTGCATTCTA GTTGTGGTTT
  901 GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAA
  961 TTCGCGTTTA AATTTTTTGT AAATCCAGCT CCATTTTTTA TCGATTAGGA CGAAATCGGC
1021 AAATCCTTAT AATTCACAGA TAGACCGAGA TAGGGATGAA GTGTTTGATT CG
//
Primer: SV40pro_F_V2 RDB14199_A6Fdb.seq
>D03515A3_A6Fd_1_SV40pro_F_V2_D05_11.ab1
    1 GTAAACATTT TTGGAGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTTGGGCTTC
  961 GGAATCGTTT TTCCGGGACG CCGGCTGGAA TGAATCCTTC CAGCGGCGGG GATCTCATGC
 1021 CTGGAAGGTT TCCTTCGGCC AACCCTTAAG GGGGAGGCTA ACTGGAATCA CCGGGAAAGG
1081 GCGAGAT
//
Primer: CMV-Forward RDB14199_A6Fdc.seq
>D03515A3_A6Fd_1_CMV-Forward_A05_02.ab1
    1 ATTAACTCCG ACCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
  841 TCCATGGCCC GGGACTACGA CCACCTCTTC AAGCTGCTCA TCATCGGCGA CAGCGGTGTG
  901 GGCAAGAGCA GCTTGCTGTT TACGATTCGC AGACACACCC TTCTCAGCAG CTACATCACC
  961 ACAATCGGAG TGGATTTCAG ATTCGGACCT GTGAGATCAC GGGGGGAGAT GGTGAGGCCT
1021 GCAGATCTGG GCACACGCAG GCCAGAGCGC TTACCCACCT ACG
//
Primer: RDB14199_A6Fdd.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl