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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab35(S5A)

Expression vector of EGFP-tagged mouse Rab35/Rab5A swapping mutation.

Catalog number RDB14199
Resource name pEGFP-C1-mouse Rab35(S5A)
Clone info. Expression vector of EGFP-tagged mouse Rab35 mutant; a swapping mutation in the switch II region between Rab35 (70–76) and Rab5A (82–88), see Fig. 2A of Etoh, K., J. Biol. Chem. 290 (14): 9064-9074 (2015) for details.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab35 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab35

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).。
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          RDB14199 pEGFP-C1-mouse Rab35(S5A) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
          original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14199_A6Fdp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Sequence file: RDB14199_A6Fda.seq check
          >D03515A3_A6Fd_1_EGFP-C_B05_05.ab1
              1 CAGTATCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGGCC CGGGACTACG
             61 ACCACCTCTT CAAGCTGCTC ATCATCGGCG ACAGCGGTGT GGGCAAGAGC AGCTTGCTGT
            121 TACGATTCGC AGACAACACC TTCTCAGGCA GCTACATCAC CACAATCGGA GTGGATTTCA
            181 AGATTCGGAC TGTGGAGATC AACGGGGAGA AGGTGAAGCT GCAGATCTGG GACACGGCAG
            241 GGCAGGAGCG CTACCACTCC CTCGCCCCTA TGTATTATCG GGGGACCCAT GGGGTCATTG
            301 TGGTTTACGA CGTCACTAGT GCCGAGTCCT TTGTCAACGT CAAGCGATGG CTTCATGAAA
            361 TCAACCAGAA CTGTGACGAT GTGTGCCGAA TATTAGTGGG CAATAAGAAT GATGACCCTG
            421 AGCGGAAGGT GGTAGAGACA GAAGATGCCT ACAAATTTGC CGGGCAGATG GGGATCCAGC
            481 TCTTTGAGAC CAGTGCCAAG GAGAACGTCA ATGTGGAAGA GATGTTCAAC TGTATCACAG
            541 AGCTGGTTCT GCGAGCAAAG AAAGACAACT TGGCGAAACA GCAGCAGCAA CAACAGAACG
            601 ATGTGGTGAA GCTCACCAAA AACAGTAAAC GAAAGAAACG CTGCTGCTAA GTCGACGGTA
            661 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG
            721 TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA
            781 TGAATGCAAT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
            841 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTTCA CTGCATTCTA GTTGTGGTTT
            901 GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAA
            961 TTCGCGTTTA AATTTTTTGT AAATCCAGCT CCATTTTTTA TCGATTAGGA CGAAATCGGC
          1021 AAATCCTTAT AATTCACAGA TAGACCGAGA TAGGGATGAA GTGTTTGATT CG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14199_A6Fdb.seq check
          >D03515A3_A6Fd_1_SV40pro_F_V2_D05_11.ab1
              1 GTAAACATTT TTGGAGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
             61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
            601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
            901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTTGGGCTTC
            961 GGAATCGTTT TTCCGGGACG CCGGCTGGAA TGAATCCTTC CAGCGGCGGG GATCTCATGC
           1021 CTGGAAGGTT TCCTTCGGCC AACCCTTAAG GGGGAGGCTA ACTGGAATCA CCGGGAAAGG
          1081 GCGAGAT
          //
          Primer: CMV-Forward
          Sequence file: RDB14199_A6Fdc.seq check
          >D03515A3_A6Fd_1_CMV-Forward_A05_02.ab1
              1 ATTAACTCCG ACCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
            181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
            241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
            661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
            721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
            781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
            841 TCCATGGCCC GGGACTACGA CCACCTCTTC AAGCTGCTCA TCATCGGCGA CAGCGGTGTG
            901 GGCAAGAGCA GCTTGCTGTT TACGATTCGC AGACACACCC TTCTCAGCAG CTACATCACC
            961 ACAATCGGAG TGGATTTCAG ATTCGGACCT GTGAGATCAC GGGGGGAGAT GGTGAGGCCT
          1021 GCAGATCTGG GCACACGCAG GCCAGAGCGC TTACCCACCT ACG
          //
          Primer:
          Sequence file: RDB14199_A6Fdd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
          original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl