Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab35(S5A) (#RDB14199)

Expression vector of EGFP-tagged mouse Rab35/Rab5A swapping mutation.

Clone info.	Expression vector of EGFP-tagged mouse Rab35 mutant; a swapping mutation in the switch II region between Rab35 (70–76) and Rab5A (82–88), see Fig. 2A of Etoh, K., J. Biol. Chem. 290 (14): 9064-9074 (2015) for details.
Comment	Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone	pEGFP-C1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name	mouse Rab35 cDNA
Depositor|Developer	Fukuda, Mitsunori |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14199	pEGFP-C1-mouse Rab35(S5A)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

---

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab35(S5A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14199).

Reference section:

Mrozowska, P.S., Fukuda, M., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252. [link to RRC of NBRP]

Etoh, K., Fukuda M., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

---

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14199_A6Fdp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C Sequence file: RDB14199_A6Fda.seq
>D03515A3_A6Fd_1_EGFP-C_B05_05.ab1     1 CAGTATCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGGCC CGGGACTACG    61 ACCACCTCTT CAAGCTGCTC ATCATCGGCG ACAGCGGTGT GGGCAAGAGC AGCTTGCTGT   121 TACGATTCGC AGACAACACC TTCTCAGGCA GCTACATCAC CACAATCGGA GTGGATTTCA   181 AGATTCGGAC TGTGGAGATC AACGGGGAGA AGGTGAAGCT GCAGATCTGG GACACGGCAG   241 GGCAGGAGCG CTACCACTCC CTCGCCCCTA TGTATTATCG GGGGACCCAT GGGGTCATTG   301 TGGTTTACGA CGTCACTAGT GCCGAGTCCT TTGTCAACGT CAAGCGATGG CTTCATGAAA   361 TCAACCAGAA CTGTGACGAT GTGTGCCGAA TATTAGTGGG CAATAAGAAT GATGACCCTG   421 AGCGGAAGGT GGTAGAGACA GAAGATGCCT ACAAATTTGC CGGGCAGATG GGGATCCAGC   481 TCTTTGAGAC CAGTGCCAAG GAGAACGTCA ATGTGGAAGA GATGTTCAAC TGTATCACAG   541 AGCTGGTTCT GCGAGCAAAG AAAGACAACT TGGCGAAACA GCAGCAGCAA CAACAGAACG   601 ATGTGGTGAA GCTCACCAAA AACAGTAAAC GAAAGAAACG CTGCTGCTAA GTCGACGGTA   661 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG   721 TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA   781 TGAATGCAAT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC   841 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTTCA CTGCATTCTA GTTGTGGTTT   901 GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAA   961 TTCGCGTTTA AATTTTTTGT AAATCCAGCT CCATTTTTTA TCGATTAGGA CGAAATCGGC 1021 AAATCCTTAT AATTCACAGA TAGACCGAGA TAGGGATGAA GTGTTTGATT CG //
Primer: SV40pro_F_V2 Sequence file: RDB14199_A6Fdb.seq
>D03515A3_A6Fd_1_SV40pro_F_V2_D05_11.ab1     1 GTAAACATTT TTGGAGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT    61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC   601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC   721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC   781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC   841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC   901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTTGGGCTTC   961 GGAATCGTTT TTCCGGGACG CCGGCTGGAA TGAATCCTTC CAGCGGCGGG GATCTCATGC  1021 CTGGAAGGTT TCCTTCGGCC AACCCTTAAG GGGGAGGCTA ACTGGAATCA CCGGGAAAGG 1081 GCGAGAT //
Primer: CMV-Forward Sequence file: RDB14199_A6Fdc.seq
>D03515A3_A6Fd_1_CMV-Forward_A05_02.ab1     1 ATTAACTCCG ACCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG   781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA   841 TCCATGGCCC GGGACTACGA CCACCTCTTC AAGCTGCTCA TCATCGGCGA CAGCGGTGTG   901 GGCAAGAGCA GCTTGCTGTT TACGATTCGC AGACACACCC TTCTCAGCAG CTACATCACC   961 ACAATCGGAG TGGATTTCAG ATTCGGACCT GTGAGATCAC GGGGGGAGAT GGTGAGGCCT 1021 GCAGATCTGG GCACACGCAG GCCAGAGCGC TTACCCACCT ACG //
Primer: Sequence file: RDB14199_A6Fdd.seq
1

Please visit Sequencing and PCR primers for primer information.

---

References

Original, user report and related articles

original	Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252. [link to RRC of NBRP]
original	Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427. [link to RRC of NBRP]

Resource search

Go!

Link

Other resources in our bank

• mouse Rab35

---

External Database
mouse Rab35

• in KEGG Gene
• in NCBI Gene

---

Reference sequence
  

Tips

Featured content

• Rab family protein (English text)

---