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pEGFP-C1-mouse Rab35(TS/TA) (#RDB14198)

Expression vector of EGFP-tagged mouse Rab35 mutant; T76S/T81A.

Clone info. Expression vector of EGFP-tagged mouse Rab35 mutant; T76S/T81A.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab35 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14198 pEGFP-C1-mouse Rab35(TS/TA) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab35(TS/TA) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14198).

Reference section:

Mrozowska, P.S., Fukuda, M., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252. [link to RRC of NBRP]
Etoh, K., Fukuda M., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14198_A6Fdp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB14198_A6Fda.seq check
>D03515A2_A6Fd_1_EGFP-C_F04_16.ab1
    1 CCGGTATCTC GGCATGGACG AGCTGTACAG TCCGGACTCA GATCCATGGC CCGGGACTAC
   61 GACCACCTCT TCAAGCTGCT CATCATCGGC GACAGCGGTG TGGGCAAGAG CAGCTTGCTG
  121 TTACGATTCG CAGACAACAC CTTCTCAGGC AGCTACATCA CCACAATCGG AGTGGATTTC
  181 AAGATTCGGA CTGTGGAGAT CAACGGGGAG AAGGTGAAGC TGCAGATCTG GGACACGGCA
  241 GGGCAGGAGC GCTTCCGCAC CATCACCTCT TCCTATTATC GGGGGGCCCA TGGGGTCATT
  301 GTGGTTTACG ACGTCACTAG TGCCGAGTCC TTTGTCAACG TCAAGCGATG GCTTCATGAA
  361 ATCAACCAGA ACTGTGACGA TGTGTGCCGA ATATTAGTGG GCAATAAGAA TGATGACCCT
  421 GAGCGGAAGG TGGTAGAGAC AGAAGATGCC TACAAATTTG CCGGGCAGAT GGGGATCCAG
  481 CTCTTTGAGA CCAGTGCCAA GGAGAACGTC AATGTGGAAG AGATGTTCAA CTGTATCACA
  541 GAGCTGGTTC TGCGAGCAAA GAAAGACAAC TTGGCGAAAC AGCAGCAGCA ACAACAGAAC
  601 GATGTGGTGA AGCTCACCAA AAACAGTAAA CGAAAGAAAC GCTGCTGCTA AGTCGACCAT
  661 AGCGGCCGCC TGCAGGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC TAGATAACTG
  721 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC
  781 CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA
  841 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  901 TTCACTGCAT TCTAGTTGTG GTTTGTCCCA AACTCATCAA TGTATCTTTA ACGCGTAAAT
  961 TTGTAAGCGT TTAATATTTT GATAAAATTC GCGTTTAATT TTTTGGTAAT CAGCTCATTT
1021 TTAATCAATA GACGGAGTCG GTCAAATCCA TATAATTCCA CAGAATTAG
//
Primer: SV40pro_F_V2
Sequence file: RDB14198_A6Fdb.seq check
>D03515A2_A6Fd_1_SV40pro_F_V2_H04_22.ab1
    1 GTAGCCTTTT TTGGAGGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCCGCC TTCTATGAAA GGTTGGCTTC
  961 GGATCGTTTT TCCCGGGAAC GCCGGCTGGA TGATCCTTCC AGCCGCGGGG AATCTTCATG
 1021 CCTTGAAGTC CTCGCCCACC CTAGGGGGAG CTAACTGTAA CACGGAATGA GAACATACCG
1081 G
//
Primer: CMV-Forward
Sequence file: RDB14198_A6Fdc.seq check
>D03515A2_A6Fd_1_CMV-Forward_E04_13.ab1
    1 CTTTACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
  841 TCCATGGCCC GGGACTACGA CCACCTCTTC AAGCTGCTCA TCATCGGCGA CAGCGGTGTG
  901 GGCAAGAGCA GCTTGCTGTT ACGATTCGCA GACAACACCC TTCTCAGGCA GCTACATCAA
  961 CCACAATCGG AGTGGATTTT CAAGAATTCG GACTGTGGTA GATCAACGGG AGGATGTTGC
1021 AAGCTGCAGA TCTGGGACAC GGCAGGCCAG GAGCG
//
Primer:
Sequence file: RDB14198_A6Fdd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252. [link to RRC of NBRP]
original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427. [link to RRC of NBRP]