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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-mouse Rab35(TS/TA)

Expression vector of EGFP-tagged mouse Rab35 mutant; T76S/T81A.

Catalog number RDB14198
Resource name pEGFP-C1-mouse Rab35(TS/TA)
Clone info. Expression vector of EGFP-tagged mouse Rab35 mutant; T76S/T81A.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab35 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab35

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).。
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          RDB14198 pEGFP-C1-mouse Rab35(TS/TA) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
          original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14198_A6Fdp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Sequence file: RDB14198_A6Fda.seq check
          >D03515A2_A6Fd_1_EGFP-C_F04_16.ab1
              1 CCGGTATCTC GGCATGGACG AGCTGTACAG TCCGGACTCA GATCCATGGC CCGGGACTAC
             61 GACCACCTCT TCAAGCTGCT CATCATCGGC GACAGCGGTG TGGGCAAGAG CAGCTTGCTG
            121 TTACGATTCG CAGACAACAC CTTCTCAGGC AGCTACATCA CCACAATCGG AGTGGATTTC
            181 AAGATTCGGA CTGTGGAGAT CAACGGGGAG AAGGTGAAGC TGCAGATCTG GGACACGGCA
            241 GGGCAGGAGC GCTTCCGCAC CATCACCTCT TCCTATTATC GGGGGGCCCA TGGGGTCATT
            301 GTGGTTTACG ACGTCACTAG TGCCGAGTCC TTTGTCAACG TCAAGCGATG GCTTCATGAA
            361 ATCAACCAGA ACTGTGACGA TGTGTGCCGA ATATTAGTGG GCAATAAGAA TGATGACCCT
            421 GAGCGGAAGG TGGTAGAGAC AGAAGATGCC TACAAATTTG CCGGGCAGAT GGGGATCCAG
            481 CTCTTTGAGA CCAGTGCCAA GGAGAACGTC AATGTGGAAG AGATGTTCAA CTGTATCACA
            541 GAGCTGGTTC TGCGAGCAAA GAAAGACAAC TTGGCGAAAC AGCAGCAGCA ACAACAGAAC
            601 GATGTGGTGA AGCTCACCAA AAACAGTAAA CGAAAGAAAC GCTGCTGCTA AGTCGACCAT
            661 AGCGGCCGCC TGCAGGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC TAGATAACTG
            721 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC
            781 CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA
            841 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
            901 TTCACTGCAT TCTAGTTGTG GTTTGTCCCA AACTCATCAA TGTATCTTTA ACGCGTAAAT
            961 TTGTAAGCGT TTAATATTTT GATAAAATTC GCGTTTAATT TTTTGGTAAT CAGCTCATTT
          1021 TTAATCAATA GACGGAGTCG GTCAAATCCA TATAATTCCA CAGAATTAG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14198_A6Fdb.seq check
          >D03515A2_A6Fd_1_SV40pro_F_V2_H04_22.ab1
              1 GTAGCCTTTT TTGGAGGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
            901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCCGCC TTCTATGAAA GGTTGGCTTC
            961 GGATCGTTTT TCCCGGGAAC GCCGGCTGGA TGATCCTTCC AGCCGCGGGG AATCTTCATG
           1021 CCTTGAAGTC CTCGCCCACC CTAGGGGGAG CTAACTGTAA CACGGAATGA GAACATACCG
          1081 G
          //
          Primer: CMV-Forward
          Sequence file: RDB14198_A6Fdc.seq check
          >D03515A2_A6Fd_1_CMV-Forward_E04_13.ab1
              1 CTTTACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
            121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
            181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
            241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
            361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
            601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
            661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
            721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            781 TCGTGACCGC CGCCGGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
            841 TCCATGGCCC GGGACTACGA CCACCTCTTC AAGCTGCTCA TCATCGGCGA CAGCGGTGTG
            901 GGCAAGAGCA GCTTGCTGTT ACGATTCGCA GACAACACCC TTCTCAGGCA GCTACATCAA
            961 CCACAATCGG AGTGGATTTT CAAGAATTCG GACTGTGGTA GATCAACGGG AGGATGTTGC
          1021 AAGCTGCAGA TCTGGGACAC GGCAGGCCAG GAGCG
          //
          Primer:
          Sequence file: RDB14198_A6Fdd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
          original Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl