RDB14183 - pcDNA3-mREV-erb alpha

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pcDNA3-mREV-erb alpha (#RDB14183)

Expression vector of mouse REV-erb alpha.

Alternative name	41
Clone info.	Expression vector of mouse REV-erb alpha.
Comment	Expression was confirmed by the depositor with real-time quantitative RT-PCR. NM_145434 8CDS 75-1922)
Vector backbone	pcDNA3 (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	mouse REV-ERB alpha cDNA
Depositor\|Developer	Takumi, Toru \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14183	pcDNA3-mREV-erb alpha	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-mREV-erb alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14183).

Reference section:

Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14183_A6F3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB14183_A6F3a.seq
>D03420B6_A6F3_1_CMV-Forward_A05_02.ab1 1 NNNNNNNNNN NNNNNTNNNN NNNNNGGGCG GTANGCGTGT NCGGNGGGNN GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG 181 TGTGCTGGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CGGGAATTCG ATTCATGACG 241 ACCCTGGACT CCAATAACAA CACAGGTGGT GTTATCACCT ACATTGGCTC TAGTGGCTCC 301 TCCCCGAGCC GGACCAGCCC GGAGTCCCTC TACAGTGACA GCTCCAATGG CAGCTTCCAG 361 TCCCTGACTC AAGGTTGTCC CACATACTTC CCACCATCAC CTACTGGCTC CCTCACCCAG 421 GACCCTGCCC GCTCTTTTGG CAGTGCGCCA CCCAGTCTCA GTGATGATAG CTCCCCTTCT 481 TCTGCATCAT CGTCATCCTC TTCATCCTCC TCCTCCTTCT ATAACGGGAG CCCCCCAGGA 541 AGTCTACAAG TGGCCATGGA AGACAGCAGC CGAGTGTCCC CCAGCAAGGG CACAAGCAAC 601 ATTACCAAGC TGAATGGCAT GGTGCTACTG TGTAAGGTGT GTGGGGACGT GGCCTCAGGC 661 TTCCACTATG GAGTGCACGC CTGTGANGGC TGCAAGGGCT TTTTTCGCCG GANCATCCAA 721 CAGAATATCN AGTACAAACG GTGTCTGAAA AACGAAAACT GCTCCATCGT TCGCATCAAT 781 CGCAACCGCT GCCAGCANTG TCGCNTCAAG AANTGTCTCT NCGTTGGCAT GTCTAGAGAT 841 GCTGNGCGTT TNNGNCNNAT NCCCCNAGAN ANANANNNNA NCGNNNNCNN GNNGANNTGC 901 NNNNCGCNAT NNNNNNNNNC NANNANCCAN CTNNNCNNCN NNNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert 3' Sequence file: RDB14183_A6F3b.seq
>D03420B6_A6F3_1_BGH_rev2_B05_05.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNG GGNNAACAAC AGNTGGCTGG CNACTAGAAG 61 GCACAGTCGA GGCTGATCAG CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT 121 CTAGATGCAT GCTCGAGCGG CCGCGAATTC ACTAGTGATT TCACTGGGCG TCCACCCGGA 181 AGGACAGCAG CTTCTCGGAA TGCATGTTGT TCAGGGTCCG CAGGTCCGGC AGCTTGAGCA 241 GCAGCTTGGT GAAGCGGGAA GTCTCCGAGG GCCGGTTCTT CAGCACCAGA GCCCGAAGAG 301 CCCGCAGCAG CGTCTCCTGG AGCTGCTCCA CCGAAGCGGA ATTCTCCATT CCCGAGCGGT 361 CTGCAGAGAC AAGCACCACC GCGGTGAATA GGCCCAGCTC CTCCTCGGTA AGTGCCAGGG 421 AGTTGAGCTT CTCGCTGAAG TCAAACATGG CATTGAGCAG GTCGCCCATG CCCATGGCAC 481 CGAGCTCCTG CAGACTGTAG GTTGTGCGGC TCAGGAACAT CACTGTCTGG TCCTTCACGT 541 TGAACAACGA TGCAAAGCGC ACCATCAGCA CCTCAAAGGT GCCAGCCTTA AGCAGGGTCA 601 CCTGGTCGTG CTGAGAAAGG TCACGGAAGC CTGGGATGTG TTTGGCAAAC TCTACCACCT 661 CCCGAACGGC AGGCGTGAAG CTCATAGAGA AGTCTTCCCA GATCTCCTGC ACAGTCCGGC 721 CGCTGCGTCC ATGGGGATAC ATGTTCATGG GACATGCNAG CAGAACATTC TTGGTGTTGC 781 CTTGCCGTAN ANTGTTGGCA NGTGCCTCCC CTTCTGGGGC CGAATATACG TGGGTGGGGC 841 ACAGACGGNG CCNATTGCTG TTANGTTGGT GGCANCTGNG GNGNGTNNNN NNGAANNNCC 901 NNNTANGCGG GTANNANNNA NNGNCCNNNN NNTAGANNNN TNCNNNGNNN NNNNNNN //
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB14183_A6F3c.seq
>D03420B6_A6F3_1_SV40pro_F_C05_08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT TTGGNNGCCT ANGCTTTTGC 61 NNNNNGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTNCC GAATATCATG 721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 781 TATCAGGACA TAGCGTTGGC TACCCGNGAT ATTGCTGAAN ANCTTGNCGG CNAATGGGGC 841 TGNNCGCTTC CTCGNGCTTT ACNGNATCGC CNCTCCNGAT NNNCANCGCN TCNNNNNNNA 901 TCNNCNTTNN NNNCGANTTC NTNNNGANCN NGNNTCNGGG GGNTNCNAAA TGNNNNNNNN 961 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

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Other resources in our bank

mouse REV-ERB alpha

External Database
mouse REV-ERB alpha

Reference sequence