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pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut) (#RDB14182)

Reporter plasmid of E-box (E1 and E2 mutants) of human Baml 1 promoter.

Clone info. Expression vector of SV40 promoter driving a firefly luciferase with E-box (-829, E1 mut/E2 mut) of human Baml 1 promoter (+1 is the putative transcription start site).
Comment Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14182 pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14182).

Reference section:

Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14182_A6F3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB14182_A6F3a.seq check
>D03420B5_A6F3_1_pGL4-4174F_G04_19.ab1
    1 NNNNNNNNNN NNTTNNNNNN TCGNNANGTN NCGNNCTCTT ACGCGTGCTA GCCCGGGCTC
   61 GAGTATTCTC TTTGGAACTA CGGGAATGAC AGCTCCAGTG GGACACTTGG AGGTCATGAT
  121 GAACGCAAAG AACGTGAGAA CACTTGCAGT CCTCGGGGTG GAAATGCCTT CTAGAAATAC
  181 TAAGTATCCG TTCTCTCGTT GAGACTTTGG CAAACCAGGG ATTTTAGGAA GGGTCTGGCA
  241 CTCAACGTTC CTAAAATTGG TTTCCTAGAT GGAGCCGGAG GGAGAGAGGG AGTCAGGAAC
  301 TGCTGCTTTT CGTCGGAATG CCCGGAGGGG AGAAGAAGAA GGAGGAGGGG TGAGGGCGGG
  361 CGGCCAAACG CCAGCCGGCA GGGGACCCAG AGAAGAGGGA CATCCCGGGC GACCCCGAGG
  421 AGCGCGGCTT GGGCACCGCA GTGGCCGCGG CTAGTGGGAG ACCTGAGGGG AAAGGGAGAG
  481 GGCAGGGGCG AGGAACCCAG GGAGCGCGCG CGGATTGGTC CTCTCCTCGG GGCGTGCGCT
  541 CCTGTGCGCC AAATGATTGG TGGCAGGAAA GTAGCAGGTA AACCGGCTCC CGCCGCCTCG
  601 CCATTGGTCA GCGGCCTCTC TAGCCGCCAC ACAACTGGCC AGCGGGCTGC CGAGCCGCGC
  661 GCGGATTGGC TGGGGGCGGC CGCCGGGACC GGCTCCCTTC GGGCGTTCGG ATTGGCTGGC
  721 GGGAANANGC ANGTATCCGG GCGCTGCGGC TCCTCCATTG GTGGGCGGGG AANGGGGGTT
  781 GGGCACAGCG ATTGGTNGNC GGGGGGGCCG GGCCTGGGCC GGCGGGGNNC GGNNTGGNCN
  841 GAAANTGCTA NCGTGGNNCG ACATTTNNNG NANNNNANAA NNNNNCNANG GNNNNNNNNN
901 N
//
Primer: Luc_R (Pr0146)
Region: Luc 5',insert 3'
Sequence file: RDB14182_A6F3b.seq check
>D03420B5_A6F3_1_Luc_R_H04_22.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
   61 TCCATCTTCC AGCGGNTAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
  121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
  181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCCTCGAG ACTTTCTGCC
  241 TTCCCTAAAT GTCGCACCAC GCTAGCACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
  301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
  361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
  421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
  481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
  541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
  601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCANG TCTCCCACTA
  661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGGGGT CGCCCGGGAN GTCCCTCTTC
  721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
  781 TCCCCTCCGG GCATTCCGAC NAAAAGCAGC AGNNCCTGAC TCCCTCTCTC CCTCCGGCTN
  841 CATCTANGAA ANNNATTTTA NGAACGTTNN NNNNCAGANC CNNCCNNAAN TCCNNNGNNT
901 NNCCAANNTC TCAACNANNN NNNNGNNNNN TNNNNNTNCN NNNNNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40pA,Luc 3'
Sequence file: RDB14182_A6F3c.seq check
>14182_14182_B0J6F_SV40pA_R_H06_24_ABI24.ab1
    1 NNNNNNNNNN NCNCNNATTT NNNNAATAAA GCATTTTTTT NNCTGCATTC TAGTTGTGGT
   61 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGCTCGA AGCGGCCGGC CGCCCCGACT
  121 CTAGAATTAC ACGGCGATCT TTCCGCCCTT CTTGGCCTTT ATGAGGATCT CTCTGATTTT
  181 TCTTGCGTCG AGTTTTCCGG TAAGACCTTT CGGTACTTCG TCCACAAACA CAACTCCTCC
  241 GCGCAACTTT TTCGCGGTTG TTACTTGACT GGCGACGTAA TCCACGATCT CTTTTTCCGT
  301 CATCGTCTTT CCGTGCTCCA AAACAACAAC GGCGGCGGGA AGTTCACCGG CGTCATCGTC
  361 GGGAAGACCT GCGACACCTG CGTCGAAGAT GTTGGGGTGT TGGAGCAAGA TGGATTCCAA
  421 TTCAGCGGGA GCCACCTGAT AGCCTTTGTA CTTAATCAGA GACTTCAGGC GGTCAACGAT
  481 GAAGAAGTGT TCGTCTTCGT CCCAGTAAGC TATGTCTCCA GAATGTAGCC ATCCATCCTT
  541 GTCAATCAAG GCGTTGGTCG CTTCCGGATT GTTTACATAA CCGGACATAA TCATAGGACC
  601 TCTCACACAC AGTTCGCCTC TTTGATTAAC GCCCAGCGTT TTCCCGGTAT CCAGATCCAC
  661 AACCTTCGCT TCAAAAAATG GAACAACTTT ACCGACCGCG CCCGGTTTAT CATCCCCCTC
  721 GGGTGTAATC AGAATAGCTG ATGTAGTCTC AGTGAGCCCA TATCCTTGCC TGATACCTGG
  781 CAGATGGAAC CTCTTGGCAA CCGCTTCCCC GACTTCCTTA GAGAGGGGAG CGCCACCAGA
  841 AGCAATTTCG TGTAAATTAN ATAAATCGNA TTTGTCAATC ANANNGCTTT TGGCGAANAA
  901 NGANAATANG GTTGGCACCN GCAGCGCACT TTGAATCNTG NAANCCNGNA NNNNTNNNCA
961 NAANNAGCNC NTCNTNNNNN NNTACNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]