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pGL3_hBmalPro-Luc (-829_+57) (#RDB14177)

Reporter plasmid of human Baml 1 promoter.

Alternative name 10
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -829 to +57 (+1 is the putative transcription start site).
Comment Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14177 pGL3_hBmalPro-Luc (-829_+57) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3_hBmalPro-Luc (-829_+57) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14177).

Reference section:

Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14177_A6F3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB14177_A6F3a.seq check
>D03420A9_A6F3_1_pGL4-4174F_F03_18.ab1
    1 NNNNNNNNNN NNTNNNNNNT CGNNAGGTNN CGNNCTCTTA CGCGTGCTAG CCCGGGCTCG
   61 AGTATTCTCT TTGGAACTAC GGGAATGACA GCTCCAGTGG GACACTTGGA GGTCATGATG
  121 AACGCAAAGA ACGTGAGAAC ACTTGCAGTC CTCGGGGTGG AAATGCCTTC TAGAAATACT
  181 AAGTATCCGT TCTCTCGTTG AGACTTTGGC AAACCAGGGA TTTTAGGAAG GGTCTGGCAC
  241 TCAACGTTCC TAAAATTGGT TTCCTAGATG GAGCCGGAGG GAGAGAGGGA GTCAGGAACT
  301 GCTGCTTTTC GTCGGAATGC CCGGAGGGGA GAAGAAGAAG GAGGAGGGGT GAGGGCGGGC
  361 GGCCAAACGC CAGCCGGCAG GGGACCCAGA GAAGAGGGAC ATCCCGGGCG ACCCCGAGGA
  421 GCGCGGCTTG GGCACCGCAG TGGCCGCGGC TAGTGGGAGA CCTGAGGGGA AAGGGAGAGG
  481 GCAGGGGCGA GGAACCCAGG GAGCGCGCGC GGATTGGTCC TCTCCTCGGG GCGTGCGCTC
  541 CTGTGCGCCA AATGATTGGT GGCAGGAAAG TAGCAGGTAA ACCGGCTCCC GCCGCCTCGC
  601 CATTGGTCAG CGGCCTCTCT AGCCGCCACA CAACTGGCCA GCGGGCTGCC GAGCCGCGCG
  661 CGGATTGGCT GGNGGCGGCC GCCGGGACCG GCTCCCTTCG GGCGTTCGGA TTGGCTGGCG
  721 GGAAGANGCA NGTATCCGGG CGCTGCGGCT CCTCCATTGG TGGGCGGGGA ANGGGGGGGN
  781 NNGGCNCAGC GANTGNNGGG GCGGGGGGGN NNGGGCCNGG GCCGGCGGGG NNCGGNNNNG
  841 TCGGAAAGTA NGTTANNGGT GCGACATTNN NGGAANGNAG AANNNNNNTC NNNNNNNGNA
901 NNNNNCNGTT TACCNNNNNN NGNNNNNNNN NN
//
Primer: Luc_R (Pr0146)
Region: Luc 5',insert 3'
Sequence file: RDB14177_A6F3b.seq check
>D03420A9_A6F3_1_Luc_R_G03_21.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCTCTTNNT AGCCTTATGC AGTTGCTCTC CNGCGGTTCC
   61 NTCTTCCNGC GGATAGAATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATGGT
  121 GGCTTTACCA ACAGTACCGG AATGCCAAGC TTACTTAGAT CGCAGATCCC GCGGCGGCGG
  181 CGGCGGTGAG TCCGGCGCGG GTAAACAGGC ACCTCCGTCC CTGACCTACT TTCTGCCTTC
  241 CCTAAATGTC GCACCACTAA CCTACTTTCC GACCAATCCG CTCCCCGCCG GCCCAGGCCC
  301 GGCCCCCCGC CCACCAATCG CTGTGCCCAA CCCCCCTTCC CCGCCCACCA ATGGAGGAGC
  361 CGCAGCGCCC GGATACCTGC CTCTTCCCGC CAGCCAATCC GAACGCCCGA AGGGAGCCGG
  421 TCCCGGCGGC CGCCCCCAGC CAATCCGCGC GCGGCTCGGC AGCCCGCTGG CCAGTTGTGT
  481 GGCGGCTAGA GAGGCCGCTG ACCAATGGCG AGGCGGCGGG AGCCGGTTTA CCTGCTACTT
  541 TCCTGCCACC AATCATTTGG CGCACAGGAG CGCACGCCCC GAGGAGAGGA NCAATCCGCG
  601 CGCGCTCCCT GGGTTCCTCG CCCCTGCCCT CTCCCTTTCC CCTCANGTCT CCCACTAGCC
  661 GCGGCCACTG CGGTGCCCAA GCCGCGCTCC TCGGGGTCGC CCGGGNNGTC CCTCTTCTCT
  721 GGGTCCCCTG CCGGCNGGCG TTNGGCCGCC CGCCCTCACN CCTCCTCCTT CTTCTTCTCC
  781 CCTCCGGGCA TTNCGACGAA AAGCAGCAGN NCCNNNNTCC CTCTCTCCCN CCNGNNNCAT
  841 CTNNGAAANC AATTTTAGGA NNGTNNNNNG CCANNNCCNN NCNNAAANNC CNNGGNTNNN
901 NNAANNNNNN NNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40pA,Luc 3'
Sequence file: RDB14177_A6F3c.seq check
>14177_14177_B0J6F_SV40pA_R_H05_23_ABI24.ab1
    1 NNNNNNNNNN TCNNNNATTT NNNNAATAAA GCATTTTTTT NNCTGCATTC TAGTTGTGGT
   61 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGCTCGA AGCGGCCGGC CGCCCCGACT
  121 CTAGAATTAC ACGGCGATCT TTCCGCCCTT CTTGGCCTTT ATGAGGATCT CTCTGATTTT
  181 TCTTGCGTCG AGTTTTCCGG TAAGACCTTT CGGTACTTCG TCCACAAACA CAACTCCTCC
  241 GCGCAACTTT TTCGCGGTTG TTACTTGACT GGCGACGTAA TCCACGATCT CTTTTTCCGT
  301 CATCGTCTTT CCGTGCTCCA AAACAACAAC GGCGGCGGGA AGTTCACCGG CGTCATCGTC
  361 GGGAAGACCT GCGACACCTG CGTCGAAGAT GTTGGGGTGT TGGAGCAAGA TGGATTCCAA
  421 TTCAGCGGGA GCCACCTGAT AGCCTTTGTA CTTAATCAGA GACTTCAGGC GGTCAACGAT
  481 GAAGAAGTGT TCGTCTTCGT CCCAGTAAGC TATGTCTCCA GAATGTAGCC ATCCATCCTT
  541 GTCAATCAAG GCGTTGGTCG CTTCCGGATT GTTTACATAA CCGGACATAA TCATAGGACC
  601 TCTCACACAC AGTTCGCCTC TTTGATTAAC GCCCAGCGTT TTCCCGGTAT CCAGATCCAC
  661 AACCTTCGCT TCAAAAAATG GAACAACTTT ACCGACCGCG CCCGGTTTAT CATCCCCCTC
  721 GGGTGTAATC AGAATAGCTG ANGTAGTCTC AGTGAGCCCA TATCCTTGCC TGATACCTGN
  781 CAGANGGAAC CTCTTGGCAA CCGCTTCCCC GACTTCCTTA GAGAGGGGAG CGCCACCAGA
  841 AGCAATTTCG TGTAAATTAG ATAAATCGTA TTTGTCNATC ANANNGCTTT TGGCGAANAA
  901 GGANAATAGG GTTGGNACCA GCAGCGNACT TNGAANCTTG NAANCCNGNA NNNNTNNNCN
961 NNAACAGCNN NNCTTCNAAT NNNTACNTTN NNANNNNNCN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]