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pGL3_hBmalPro-Luc (-3465_Full) (#RDB14174)

Reporter plasmid of human Baml 1 promoter.

Alternative name 7
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -3465 to +57 (+1 is the putative transcription start site).
Comment Genomic DNA (13,274,365 – 13,277,883nt of NC_000011.10) corresponding to 1 to 157nt of NM_001178.5 and 3.4 kb up-stream was cloned. Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

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RDB14174 pGL3_hBmalPro-Luc (-3465_Full) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3_hBmalPro-Luc (-3465_Full) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14174).

Reference section:

Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14174_A6F3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB14174_A6F3d.seq check
>D03420A6_A6F3_1_pGL4-4174F_E09_15.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGNTAG GTNCCGAGCT CTTACGCGTG CTAGCCCGGG
   61 CTCGAGATCC AATCATTGGA TCAGAGTCAG GGAAACTACT GTAGATTATA CTACTTTATC
  121 CTCACTAACT TCATCTGTAA AACAGGAGCA AGAAATATCT ATTTCATAGA ATTGTTAAGA
  181 GTGATACCTA ATATGTATCA CATCTTGACT GTATGCCAGG CACTGTGTTA AGGGCTTTCC
  241 ATCTATCATC ACATTGAACT CTTATAACAG CCCTATGAAG TAGACACTAG ATTATCCCCC
  301 TTTTACAGAT GAGGAAACTG AGGTACAAAG AGGTTAAGCA ATTTGATAAA AGTCATCCAG
  361 CTAGCAAGTG TGGAGGGCAA GCATATCAAC TGCTAGGGTT AACGTGAGGA TTATATTAAA
  421 TAATGTATTT AAAGCATCTG GTACACAGTA GGTGCAAATA AATGCTCTCT CTTATCTTCC
  481 TTTTATCCAC ATGATTCTAC TCATGCCCAG TAAAATTTAC ATTGAGGACA ATATTTTGAC
  541 CTTAGCAGTG CCCTATGAAT TGAAAGTTCA GTATCCTGGA AAGTAGAAAA GAAAAGGGAA
  601 GTTAGGTAGT AGTGGAAGGA GGTGTTTGGG ATCTCCAACA GAGAGAACCA CAAAGAAAGG
  661 TGTAATTACA CAGGGAAAAA CAGGTGCACA TGAGGGAAGA CAGTTGCTTG NCCACTGAGG
  721 GTAGGATAAG GCAGGGTGAT AACCTTTAAA AGAGGAAAGC CAGGGTACAA GATGAGAATC
  781 TCAGANGAAG GGAGTCCACA AAGACACCTA TTTCAGCCCG CCTCCCACTG CATGGNAATC
  841 CAATTCTCAC TTGNNCATTT NNTGTGACNA GAAACTCAAT TAATCAAGGC AGAGGANGCT
  901 CTANCTGNTA NAAAANTGTN CNTATNGATT CANNCTNNAN TTTCTANCNN CANGNNNNNC
  961 NNCNNCCCNN TNNNNNCCTT NAGTATNNGT TNNNNTNNNN NNCNNNTNNA NANNNNNNNN
1021 NN
//
Primer: Luc_R (Pr0146)
Region: luc,insert 3'
Sequence file: RDB14174_A6F3e.seq check
>D03420A6_A6F3_1_Luc_R_H01_22.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
   61 TCCNTCTTCC AGCGGANAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
  121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
  181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCTGACCT ACTTTCTGCC
  241 TTCCCTAAAT GTCGCACCAC TAACCTACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
  301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
  361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
  421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
  481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
  541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
  601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCAGG TCTCCCACTA
  661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGNGGT CGCCCGGGAT GTCCCTCTTC
  721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
  781 TCCCCTCCGG GCATTCCGAC GNNNNGCAGC ANTTCCNGAC TCCCTCTCTC CCTCNGGCTN
  841 NNTCTANGAA ANCNNNTTTN ANGNNCGTTN NNNGCCAGAN CCCNTNNCNA AAANNCCCNN
901 GNNTNNNCAA ANNNNNNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]