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pGL3_hBmalPro-Luc (-3465_Full)

Reporter plasmid of human Baml 1 promoter.

Catalog number RDB14174
Resource name pGL3_hBmalPro-Luc (-3465_Full)
Alternative name 7
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -3465 to +57 (+1 is the putative transcription start site).
Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor Takumi, Toru |
 
Other clones in our bank human ARNTL (NCBI Gene 406) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB14174 pGL3_hBmalPro-Luc (-3465_Full) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Circadian clock (English text)

References

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14174_A6F3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB14174_A6F3a.seq
>D03420A6_A6F3_1_pGL4-4174F_E09_15.ab1
    1 ATAGGGGTCA GGACATTTCT CTATCGATAG GTACCGAGCT CTTACGCGTG CTAGCCCGGG
   61 CTCGAGATCC AATCATTGGA TCAGAGTCAG GGAAACTACT GTAGATTATA CTACTTTATC
  121 CTCACTAACT TCATCTGTAA AACAGGAGCA AGAAATATCT ATTTCATAGA ATTGTTAAGA
  181 GTGATACCTA ATATGTATCA CATCTTGACT GTATGCCAGG CACTGTGTTA AGGGCTTTCC
  241 ATCTATCATC ACATTGAACT CTTATAACAG CCCTATGAAG TAGACACTAG ATTATCCCCC
  301 TTTTACAGAT GAGGAAACTG AGGTACAAAG AGGTTAAGCA ATTTGATAAA AGTCATCCAG
  361 CTAGCAAGTG TGGAGGGCAA GCATATCAAC TGCTAGGGTT AACGTGAGGA TTATATTAAA
  421 TAATGTATTT AAAGCATCTG GTACACAGTA GGTGCAAATA AATGCTCTCT CTTATCTTCC
  481 TTTTATCCAC ATGATTCTAC TCATGCCCAG TAAAATTTAC ATTGAGGACA ATATTTTGAC
  541 CTTAGCAGTG CCCTATGAAT TGAAAGTTCA GTATCCTGGA AAGTAGAAAA GAAAAGGGAA
  601 GTTAGGTAGT AGTGGAAGGA GGTGTTTGGG ATCTCCAACA GAGAGAACCA CAAAGAAAGG
  661 TGTAATTACA CAGGGAAAAA CAGGTGCACA TGAGGGAAGA CAGTTGCTTG GCCACTGAGG
  721 GTAGGATAAG GCAGGGTGAT AACCTTTAAA AGAGGAAAGC CAGGGTACAA GATGAGAATC
  781 TCAGAGGAAG GGAGTCCACA AAGACACCTA TTTCAGCCCG CCTCCCACTG CATGGTAATC
  841 CAATTCTCAC TTGGACATTT TTTGTGACAA GAAACTCAAT TAATCAAGGC AGAGGATGCT
  901 CTAGCTGTTA GAAAATTGTT CATATTGATT CATTCTAGAG TTTCTACCCA CAGGTTCTCC
  961 TTCAACCCCT TAAGGCCCTT AAGTATAAGT TTAATTTCTC TTCTATTTGA CAGTTCTTCA
 1021 CATATATCAG ACAGTTACCC TTTGTACCCC TGGGGGCAAA CTTCTGCTTC TTAATATTTC
 1081 CTCATATGAC ATAGTTCTAG CCTCTTTACA TTCTGATCCC TCTACTGTGC ATGTAGATAT
1141 TCCTTCCTGC CAGCTATGAC GACATACCTT CCAACG
//
Primer: Luc_R RDB14174_A6F3b.seq
>D03420A6_A6F3_1_Luc_R_H01_22.ab1
    1 AAGGGTTCAA GGTACCAGGG CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT
   61 TCCATCTTCC AGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
  121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
  181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCTGACCT ACTTTCTGCC
  241 TTCCCTAAAT GTCGCACCAC TAACCTACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
  301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
  361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
  421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
  481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
  541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
  601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCAGG TCTCCCACTA
  661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGGGGT CGCCCGGGAT GTCCCTCTTC
  721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
  781 TCCCCTCCGG GCATTCCGAC GAAAAGCAGC AGTTCCTGAC TCCCTCTCTC CCTCCGGCTC
  841 CATCTAGGAA ACCAATTTTT AGGAACGTTG AGTGCCAGAC CCCTTTCCTA AAAATCCCTG
  901 GGTTTGCCAA AGTCTCAACG AGAGAACGGA TACTTTAGTA TTTTCTAGAA AGGCATTTCC
  961 CACCCCCGAG GACTGCAAGG TGTTTCTCAC GTTCTTTTGC GTTCATCATG AACCCTCCAA
 1021 GTGTCCCCAC TTGGAGGCTG GTCATTCCCC GTAGTACGAA GAGATTACTC GAGCGGCGGA
1081 GCCGTA
//
Primer: pAxCALNLF1 RDB14174_A6F3c.seq
>D03420A6_A6F3_1_pAxCALNLF1_A06_01_ABI08.ab1
    1 CCTAATGTTT CTTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTACA
   61 CGGCGATCTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC TCTGATTTTT CTTGCGTCGA
  121 GTTTTCCGGT AAGACCTTTC GGTACTTCGT CCACAAACAC AACTCCTCCG CGCAACTTTT
  181 TCGCGGTTGT TACTTGACTG GCGACGTAAT CCACGATCTC TTTTTCCGTC ATCGTCTTTC
  241 CGTGCTCCAA AACAACAACG GCGGCGGGAA GTTCACCGGC GTCATCGTCG GGAAGACCTG
  301 CGACACCTGC GTCGAAGATG TTGGGGTGTT GGAGCAAGAT GGATTCCAAT TCAGCGGGAG
  361 CCACCTGATA GCCTTTGTAC TTAATCAGAG ACTTCAGGCG GTCAACGATG AAGAAGTGTT
  421 CGTCTTCGTC CCAGTAAGCT ATGTCTCCAG AATGTAGCCA TCCATCCTTG TCAATCAAGG
  481 CGTTGGTCGC TTCCGGATTG TTTACATAAC CGGACATAAT CATAGGACCT CTCACACACA
  541 GTTCGCCTCT TTGATTAACG CCCAGCGTTT TCCCGGTATC CAGATCCACA ACCTTCGCTT
  601 CAAAAAATGG AACAACTTTA CCGACCGCGC CCGGTTTATC ATCCCCCTCG GGTGTAATCA
  661 GAATAGCTGA TGTAGTCTCA GTGAGCCCAT ATCCTTGCCT GATACCTGGC AGATGGAACC
  721 TCTTGGCAAC CGCTTCCCCG ACTTCCTTAG AGAGGGGAGC GCCACCAGAA GCAATTTCGT
  781 GTAAATTAGA TAAATCGTAT TTGTCAATCA GAGTGCTTTT GGCGAAGAAG GAGAATAGGG
  841 TTGGCACCAG CAGCGCACTT TGAATCTTGT AATCCTGAAG GCTCCTCAGA AACAGCTCTT
  901 CTTCAAATCT ATACATTAAG ACGACTCGAA ATCCACATAT CAAATATCCG AGTGTAGTAA
  961 CATTCCCAAA AACCGTGATG GAATGGAACA ACACTTTAAA ATCGCAGTAT CGGAATGATT
 1021 GGATGCCAAA AATAGGATCT CTGGCCATGC GAGCATCTCA CGCAGGCAGT TCCTATGGAG
1081 CAGAGTCGAC AC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

2019.01.17

GNP_filter3_RDBDEP_html_190110.pl