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pGL3_hBmalPro-Luc (-3465_Full) (#RDB14174)

Reporter plasmid of human Bmal1 promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Akashi, M., Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name 7
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Bmal1 promoter, hBmal1 region spans from -3465 to +57 (+1 is the putative transcription start site).
Comment Genomic DNA (13,274,365 – 13,277,883nt of NC_000011.10) corresponding to 1 to 157nt of NM_001178.5 and 3.4 kb up-stream was cloned. Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name human Bmal1 genomic DNA
Depositor|Developer Takumi, Toru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

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RDB14174 pGL3_hBmalPro-Luc (-3465_Full) DNA solution

How to cite this biological resource

Materials & Methods section:

The pGL3_hBmalPro-Luc (-3465_Full) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14174).

Reference section:

Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [PubMed] [Article] [RRC of NBRP]