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pGL3_hBmalPro-Luc (-3465_Full)

Reporter plasmid of human Baml 1 promoter.

Catalog number RDB14174
Resource name pGL3_hBmalPro-Luc (-3465_Full)
Alternative name 7
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -3465 to +57 (+1 is the putative transcription start site).
Comment Genomic DNA (13,274,365 – 13,277,883nt of NC_000011.10) corresponding to 1 to 157nt of NM_001178.5 and 3.4 kb up-stream was cloned. Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
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External Database
human Bmal 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          RDB14174 pGL3_hBmalPro-Luc (-3465_Full) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Circadian clock (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14174_A6F3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB14174_A6F3a.seq check
          >D03420A6_A6F3_1_pGL4-4174F_E09_15.ab1
              1 ATAGGGGTCA GGACATTTCT CTATCGATAG GTACCGAGCT CTTACGCGTG CTAGCCCGGG
             61 CTCGAGATCC AATCATTGGA TCAGAGTCAG GGAAACTACT GTAGATTATA CTACTTTATC
            121 CTCACTAACT TCATCTGTAA AACAGGAGCA AGAAATATCT ATTTCATAGA ATTGTTAAGA
            181 GTGATACCTA ATATGTATCA CATCTTGACT GTATGCCAGG CACTGTGTTA AGGGCTTTCC
            241 ATCTATCATC ACATTGAACT CTTATAACAG CCCTATGAAG TAGACACTAG ATTATCCCCC
            301 TTTTACAGAT GAGGAAACTG AGGTACAAAG AGGTTAAGCA ATTTGATAAA AGTCATCCAG
            361 CTAGCAAGTG TGGAGGGCAA GCATATCAAC TGCTAGGGTT AACGTGAGGA TTATATTAAA
            421 TAATGTATTT AAAGCATCTG GTACACAGTA GGTGCAAATA AATGCTCTCT CTTATCTTCC
            481 TTTTATCCAC ATGATTCTAC TCATGCCCAG TAAAATTTAC ATTGAGGACA ATATTTTGAC
            541 CTTAGCAGTG CCCTATGAAT TGAAAGTTCA GTATCCTGGA AAGTAGAAAA GAAAAGGGAA
            601 GTTAGGTAGT AGTGGAAGGA GGTGTTTGGG ATCTCCAACA GAGAGAACCA CAAAGAAAGG
            661 TGTAATTACA CAGGGAAAAA CAGGTGCACA TGAGGGAAGA CAGTTGCTTG GCCACTGAGG
            721 GTAGGATAAG GCAGGGTGAT AACCTTTAAA AGAGGAAAGC CAGGGTACAA GATGAGAATC
            781 TCAGAGGAAG GGAGTCCACA AAGACACCTA TTTCAGCCCG CCTCCCACTG CATGGTAATC
            841 CAATTCTCAC TTGGACATTT TTTGTGACAA GAAACTCAAT TAATCAAGGC AGAGGATGCT
            901 CTAGCTGTTA GAAAATTGTT CATATTGATT CATTCTAGAG TTTCTACCCA CAGGTTCTCC
            961 TTCAACCCCT TAAGGCCCTT AAGTATAAGT TTAATTTCTC TTCTATTTGA CAGTTCTTCA
           1021 CATATATCAG ACAGTTACCC TTTGTACCCC TGGGGGCAAA CTTCTGCTTC TTAATATTTC
           1081 CTCATATGAC ATAGTTCTAG CCTCTTTACA TTCTGATCCC TCTACTGTGC ATGTAGATAT
          1141 TCCTTCCTGC CAGCTATGAC GACATACCTT CCAACG
          //
          Primer: Luc_R
          Region: Luc 5',insert 3'
          Sequence file: RDB14174_A6F3b.seq check
          >D03420A6_A6F3_1_Luc_R_H01_22.ab1
              1 AAGGGTTCAA GGTACCAGGG CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT
             61 TCCATCTTCC AGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
            121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
            181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCTGACCT ACTTTCTGCC
            241 TTCCCTAAAT GTCGCACCAC TAACCTACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
            301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
            361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
            421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
            481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
            541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
            601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCAGG TCTCCCACTA
            661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGGGGT CGCCCGGGAT GTCCCTCTTC
            721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
            781 TCCCCTCCGG GCATTCCGAC GAAAAGCAGC AGTTCCTGAC TCCCTCTCTC CCTCCGGCTC
            841 CATCTAGGAA ACCAATTTTT AGGAACGTTG AGTGCCAGAC CCCTTTCCTA AAAATCCCTG
            901 GGTTTGCCAA AGTCTCAACG AGAGAACGGA TACTTTAGTA TTTTCTAGAA AGGCATTTCC
            961 CACCCCCGAG GACTGCAAGG TGTTTCTCAC GTTCTTTTGC GTTCATCATG AACCCTCCAA
           1021 GTGTCCCCAC TTGGAGGCTG GTCATTCCCC GTAGTACGAA GAGATTACTC GAGCGGCGGA
          1081 GCCGTA
          //
          Primer: pAxCALNLF1
          Region: SV40pA,Luc 3'
          Sequence file: RDB14174_A6F3c.seq check
          >D03420A6_A6F3_1_pAxCALNLF1_A06_01_ABI08.ab1
              1 CCTAATGTTT CTTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTACA
             61 CGGCGATCTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC TCTGATTTTT CTTGCGTCGA
            121 GTTTTCCGGT AAGACCTTTC GGTACTTCGT CCACAAACAC AACTCCTCCG CGCAACTTTT
            181 TCGCGGTTGT TACTTGACTG GCGACGTAAT CCACGATCTC TTTTTCCGTC ATCGTCTTTC
            241 CGTGCTCCAA AACAACAACG GCGGCGGGAA GTTCACCGGC GTCATCGTCG GGAAGACCTG
            301 CGACACCTGC GTCGAAGATG TTGGGGTGTT GGAGCAAGAT GGATTCCAAT TCAGCGGGAG
            361 CCACCTGATA GCCTTTGTAC TTAATCAGAG ACTTCAGGCG GTCAACGATG AAGAAGTGTT
            421 CGTCTTCGTC CCAGTAAGCT ATGTCTCCAG AATGTAGCCA TCCATCCTTG TCAATCAAGG
            481 CGTTGGTCGC TTCCGGATTG TTTACATAAC CGGACATAAT CATAGGACCT CTCACACACA
            541 GTTCGCCTCT TTGATTAACG CCCAGCGTTT TCCCGGTATC CAGATCCACA ACCTTCGCTT
            601 CAAAAAATGG AACAACTTTA CCGACCGCGC CCGGTTTATC ATCCCCCTCG GGTGTAATCA
            661 GAATAGCTGA TGTAGTCTCA GTGAGCCCAT ATCCTTGCCT GATACCTGGC AGATGGAACC
            721 TCTTGGCAAC CGCTTCCCCG ACTTCCTTAG AGAGGGGAGC GCCACCAGAA GCAATTTCGT
            781 GTAAATTAGA TAAATCGTAT TTGTCAATCA GAGTGCTTTT GGCGAAGAAG GAGAATAGGG
            841 TTGGCACCAG CAGCGCACTT TGAATCTTGT AATCCTGAAG GCTCCTCAGA AACAGCTCTT
            901 CTTCAAATCT ATACATTAAG ACGACTCGAA ATCCACATAT CAAATATCCG AGTGTAGTAA
            961 CATTCCCAAA AACCGTGATG GAATGGAACA ACACTTTAAA ATCGCAGTAT CGGAATGATT
           1021 GGATGCCAAA AATAGGATCT CTGGCCATGC GAGCATCTCA CGCAGGCAGT TCCTATGGAG
          1081 CAGAGTCGAC AC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl