RDB14145 - pCMV-TUBA1A myc-R64W

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pCMV-TUBA1A myc-R64W (#RDB14145)

Expression vector of Myc-tagged human TUBA1A mutant, R64W.

Clone info.	Expression vector of Myc-tagged human TUBA1A mutant, R64W.
Comment	Expression was confirmed by the depositor with immunofluorescence.
Vector backbone	pCMV-Tag 4 (Plasmid)
Size of vector backbone	4.3 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human tubulin alpha 1a cDNA
Depositor\|Developer	Kurahashi, Hiroki \| Yokoi, Setsuri \| Tsutsumi, Makiko \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14145	pCMV-TUBA1A myc-R64W	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-TUBA1A myc-R64W was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14145).

Reference section:

Yokoi, S., Ishihara, N., Miya, F., Tsutsumi, M., Yanagihara, I., Fujita, N., Yamamoto, H., Kato, M., Okamoto, N., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Kojima, S., Saitoh, S., Kurahashi, H., Natsume, J., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14145_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14145_A6Dpa.seq
>D03180A6_A6Dp_1_CMV-Forward_D10_10.ab1 1 ATTCACTCCG ACCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC 121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC 181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC 241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA 301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCTGG 361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC 421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC 481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG 541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA 601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG 661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC 721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA 781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT 841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT 901 GATGGAGCCC TGAAATGTTG ACCTGACAGA ATTTCCAGAC CAAACCCTGG TGCCCTATCC 961 CCGCATCCAC TTTCCCTCTG GCCACATATG CCCCTGTCAT CCTCCTGCCT GGAGAAAGGC 1021 TACCATGGAC CAGCCTTTCC TGGTAGCAGA AGATCACGAT GCTGCCTTTT GAGCCCAGTG 1081 AACCAAGAAT TGAGT //
Primer: T7_20bp Sequence file: RDB14145_A6Dpb.seq
>D03180A6_A6Dp_1_T7_20bp_E10_13.ab1 1 TATTACTGGT ACTTATTATT AAGGTACCGG GCCCTACAGA TCCTCTTCTG AGATGAGTTT 61 TTGTTCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCCTGCA GGTATTCCTC 121 TCCTTCTTCC TCACCCTCTC CTTCAACAGA ATCCACACCA ACCTCCTCAT AATCCTTCTC 181 AAGGGCAGCC ATGTCCTCAC GGGCCTCTGA AAACTCACCT TCCTCCATCC CCTCCCCAAC 241 GTACCAGTGA ACAAAGGCAC GTTTGGCATA CATCAGGTCA AACTTGTGGT CCAGGCGAGC 301 CCAGGCCTCA GCAATGGCTG TGGTGTTGCT CAGCATGCAC ACAGCTCTCT GTACCTTGGC 361 CAGGTCTCCA CCAGGCACCA CAGTGGGAGG CTGGTAGTTG ATGCCAACCT TGAAGCCAGT 421 GGGGCACCAA TCCACAAACT GGATGGTACG CTTGGTCTTG ATGGTGGCAA TGGCAGCATT 481 GACATCTTTG GGAACCACGT CACCACGGTA CAACAGGCAG CAAGCCATGT ATTTACCATG 541 GCGAGGGTCA CATTTCACCA TCTGGTTGGC TGGCTCAAAG CAAGCATTGG TGATCTCTGC 601 TACAGAAAGC TGTTCATGGT AGGCTTTCTC AGCAGAGATG ACAGGGGCAT ATGTGGCCAG 661 AGGGAAGTGG ATGCGGGGAT AGGGCACCAG GTTGGTCTGG AATTCTGTCA GGTCAACATT 721 CAGGGCTCCA TCAAATCTCA GGGAAGCAGT GATGGAGGAC ACAATTTGAC CTATTAACCT 781 ATTCAGGTTA GTATAGGTTG GACGCTCAAT ATCGAGGTTT CTACGACAGA TGTCATAGAT 841 GGCCTCATTG TCTACCATGA AGGCACAATC AGAGTGCTCC AGGGTGGTGT GGGTGGTGAG 901 AGAGTAAAGA GAGTGGGTTG //
Primer: SV40pro_F_V2 Sequence file: RDB14145_A6Dpc.seq
>D03180A6_A6Dp_1_SV40pro_F_V2_F10_16.ab1 1 GGGAACTTTT TTGGGAGGCT AGGCTTTTGC AAGATCGATC AAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAA CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAA CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC 901 GCCCAACCTG CCATCACGAG ATTTCGATTC CCACCCGCCG CCCTTCTATG AAAGGTTGGG 961 CTTCGGAATC GTTTTTCGGG AACGGCCGGG CTGGAATGAT CCTTCAGCCG CCGGGGATCT 1021 CATGGCTGGA AGTCCATCGC CACCCTTAGG GGAAGGCTAA CTGCAACACG AAGAGACCAA 1081 TACCGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yokoi, S., TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis. Sci. Rep. 5: 15165 (2015). PMID 26493046. [link to RRC of NBRP]

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human tubulin alpha 1a

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human tubulin alpha 1a

Reference sequence