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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pIDT-SMART(C-TSC)-HA-hKEAP1 (#RDB14133)

Expression vector of N-terminal HA-tagged human KEAP1.

Clone info. Expression vector of N-terminal HA-tagged human KEAP1.
Vector backbone pIDT-SMART (C-TSC) ()
Selectable markers Kan^r (E. coli).
Gene/insert name human KEAP1 cDNA
Depositor|Developer Sakaguchi, Masakiyo | Murata, Hitoshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Murata, T. et al., PLoS One 10(11):e0142438, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Murata, T. et al., PLoS One 10(11):e0142438, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14133 pIDT-SMART(C-TSC)-HA-hKEAP1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pIDT-SMART(C-TSC)-HA-hKEAP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14133).

Reference section:

Murata, H., Takamatsu, H., Liu, S., Kataoka, K., Huh, N.H., Sakaguchi, M., NRF2 Regulates PINK1 Expression under Oxidative Stress Conditions. PLoS One 10 (11): e0142438 (2015). PMID 26555609. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14133_B3Dbp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: RU5-F (Pr0383)
Region: insert 5'(HA,KEAP1 5')
Sequence file: RDB14133_B3Dba.seq check
>14133_14133_B3Db_2_RU5-F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACNGATCCNA
   61 GCCACCCCGG AATTCCGGGG AAGATCTTCC CGGGGTACCC CGAGGACTAG TTCGACGCCG
  121 GCCAAGACAG CACAGACAGA TTGACCTATT GGGGTGTTTC GCGAGTGTGA GAGGGAAGCG
  181 CCGCGGCCTG TATTACTAGA CCTGCCCTTC GCCTGGTTCG TGGCGCCTTG TGACCCCGGG
  241 CCCCTGCCGC CTGCAAGTCG AAATTGCGCT GTGCTCCTGT GCTACGGCCT GTGGCTGGAC
  301 TGCCTGCTGC TGCCCTACTG GCTGGCAAGA TCAAGCTCTC CCTGGTGGCC GCGATCCTCG
  361 CGGATCCGCG ACCATGTATC CCTATGATGT TCCCGATTAT GCTGGTCCAG GACAGCCAGA
  421 TCCCAGGCCT AGCGGGGCTG GGGCCTGCTG CCGATTCCTG CCCCTGCAGT CACAGTGCCC
  481 TGAGGGGGCA GGGGACGCGG TGATGTACGC CTCCACTGAG TGCAAGGCGG AGGTGACGCC
  541 CTCCCAGCAT GGCAACCGCA CCTTCAGCTA CACCCTGGAG GATCATACCA AGCAGGCCTT
  601 TGGCATCATG AACGAGCTGC GGCTCAGCCA GCAGCTGTGT GACGTCACAC TGCAGGTCAA
  661 GTACCAGGAT GCACCGGCCG CCCAGTTCAT GGCCCACAAG GTGGTGCTGG CCTCATCCAG
  721 CCCTGTCTTC AAGGCCATGT TCACCAACGG GCTGCGGGAG CAGGGCATGG AGGTGGTGTC
  781 CATTGAGGGT ATCCACCCCA AGGTCATGGA NCGCCTCATT GAATTCGCCT ACACGGCCTC
  841 CATCTCCNTG GGCGAGANNT GTGTCCTCNN CGTCATGAAC GGTGCTGTCA TGTANCAGAT
  901 CGACNGCGTT GTCCGTGCCT GCAGTGNCTT CCTGGTGCAN CANCTGGANC CCNGCAATGN
  961 CNTCGGCNTC GCCNANTNCN CTGANCANNN TNGNNTGNGT GGANTTNNNN CNNCGNGNCC
1021 NNGNNNNACN NCNNNNNGNA TTTNNGGGGA NGNNNNNNNN NN
//
Primer: KEAP1-B_F (Pr1316)
Region: insert 3'(KEAP 3'),bGH pA
Sequence file: RDB14133_B3Dbb.seq check
>14133_14133_B3Db_2_KEAP1-B_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNGCTGNN NCGAAGGNNC GGGGTGGGCG TGGCTGTCCT
   61 CAATCGTCTG CTTTATGCCG TGGGGGGCTT TGACGGGACA AACCGCCTTA ATTCAGCTGA
  121 GTGTTACTAC CCAGAGAGGA ACGAGTGGCG AATGATCACA GCAATGAACA CCATCCGAAG
  181 CGGGGCAGGC GTCTGCGTCC TGCACAACTG TATCTATGCT GCTGGGGGCT ATGATGGTCA
  241 GGACCAGCTG AACAGCGTGG AGCGCTACGA TGTGGAAACA GAGACGTGGA CTTTCGTAGC
  301 CCCCATGAAG CACCGGCGAA GTGCCCTGGG GATCACTGTC CACCAGGGGA GAATCTACGT
  361 CCTTGGAGGC TATGATGGTC ACACGTTCCT GGACAGTGTG GAGTGTTACG ACCCAGATAC
  421 AGACACCTGG AGCGAGGTGA CCCGAATGAC ATCGGGCCGG AGTGGGGTGG GCGTGGCTGT
  481 CACCATGGAG CCCTGCCGGA AGCAGATTGA CCAGCAGAAC TGTACCTGTT GACCGCTCGA
  541 GCGGAAAAGG CCTTTTGCTC TAGAGCTAGA TGACTAACGT TTAAACCCGC TGATCAGCCT
  601 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
  661 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
  721 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
  781 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ANGCGGTGGG CTCTATGGCG GANTACTGTC
  841 CTCCGCTTCC CACGTGGCGN ANGGNNTGGT CCTCCGCTTC CCACGTGGCG NANGGANTGG
  901 GGACCCNGGC ACCCGTCCTG NCCCTTNACC TNNCANCTCC GCCTCCTCCG CGCGGANCCN
  961 GNCCCGNNCC GANCCCTNCC GGGNNCCCGG NCCAGCCCCN NNCGGGNCCN NCNNNCCCCN
1021 NCCCNNNNNN TTNNNNNGNC CCNNNCCTNN NNNNNNNN
//
Primer: BGH_F2 (Pr0385)
Region: bGH pA,hTERT,SV40,CMV en.2
Sequence file: RDB14133_B3Dbc.seq check
>14133_14133_B3Db_2_BGH_F2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNTTGNNCCT GGNNNGNGCC NCTCCCNCTG TCCTTTCCTA
   61 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG
  121 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC
  181 GGTGGGCTCT ATGGCGGAGT ACTGTCCTCC GCTTCCCACG TGGCGGAGGG ACTGGTCCTC
  241 CGCTTCCCAC GTGGCGGAGG GACTGGGGAC CCGGGCACCC GTCCTGCCCC TTCACCTTCC
  301 AGCTCCGCCT CCTCCGCGCG GACCCCGCCC CGTCCCGACC CCTCCCGGGT CCCCGGCCCA
  361 GCCCCCTCCG GGCCCTCCCA GCCCCTCCCC TTCCTTTCCG CGGCCCCGCC CTCTCCTCGC
  421 GGCGCGAGTT TTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATCCA AAGCATCCAT
  481 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATC
  541 CAAAGCATCC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
  601 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
  661 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
  721 TTTGGAGGCC AAGGCTTTTG CAAAAAGCTC CGTTACATAA CTTACGGTAA ATGGCCCGCC
  781 TGGCTGANCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TNNCCATAGT
  841 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAN NANTTACNGN AAAANTGCCC
  901 ACTNGGCANN ACATCAAGNG TATCANATGC CNAGTACGCC CCCNATTGNN NNCANTGNNN
  961 GNAANNNGNC CCGNCTGNNA NNNNGCCNNN NNNNNGNNNC NNATGGGNNT TTCNNACTTN
1021 NNANNANNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Murata, H., NRF2 Regulates PINK1 Expression under Oxidative Stress Conditions. PLoS One 10 (11): e0142438 (2015). PMID 26555609. [link to RRC of NBRP]