RDB13972 - pClneoLuc-PP2A

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pClneoLuc-PP2A (#RDB13972)

Expression vector of luciferase-fused PP2A.

Clone info.	Expression vector of luciferase-fused PP2A.
Comment	Expression was confirmed by the depositor with luciferase activity.
Vector backbone	pCIneo (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	human PP2A cDNA firefly luciferase
Depositor\|Developer	Hata, Yutaka \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13972	pClneoLuc-PP2A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pClneoLuc-PP2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13972).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13972_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13972_A6Bta.seq
>D03116A6_A6Bt_3_CMV_Forward_H07_22.ab1 1 GATTCGCTGC GGGATCGCGC GCCCCGTTGT ACGCAAATGG GCGGTAGGCG TGTACGGTGG 61 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG 121 GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC 181 TTAAGCTGCA GTGACTCTCT TAAGGTAGCC TTGCAGAAGT TGGTCGTGAG GCACTGGGCA 241 GGTAAGTATC AAGGTTACAA GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG 301 ACAGAGAAGA CTCTTGCGTT TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC 361 CTTTCTCTCC ACAGGTGTCC ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA 421 ATACGACTCA CTATAGGCTA GCCACCATGG AAGACGCCAA AAACATAAAG AAAGGCCCGG 481 CGCCATTCTA TCCGCTGGAA GATGGAACCG CTGGAGAGCA ACTGCATAAG GCTATGAAGA 541 GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC ACATATCGAG GTGGACATCA 601 CTTACGCTGA GTACTTCGAA ATGTCCGTTC GGTTGGCAGA AGCTATGAAA CGATATGGGC 661 TGAATACAAA TCACAGAATC GTCGTATGCA GTGAAAACTC TCTTCAATTC TTTATGCCGG 721 TGTTGGGCGC GTTATTTATC GGAGTTGCAG TTGCGCCCGC GAACGACATT TATAATGAAC 781 GTGAATTGCT CAACAGTATG GGCATTTCGC AGCCTACCGT GGTGTTCGTT TCCAAAAAGG 841 GGTTGCAAAA AATTTTGAAC GTGCAAAAAA AGCTCCCAAT CATCCAAAAA ATTATTATCA 901 TGGATTCTAA AACGGGATTA CCAGGGATTT CAGTCGATGT ACACGTTCGT CACATCTCAT 961 CTACCTCCCG GGTTTTAATG AATACGATTT TGTGCCAGAG TCCCTTTCGA TAGGACAGAC 1021 ATTTGCACTG ATCATGAACT CCTCTGATCT ACTGGTCTGC CTTAACGTGT CGCTCTGGCT 1081 CATAGACTGC CTGCTGAGAT TCTCGCCATG CCAGAGATTC GTATTTTTTG GCA //
Primer: Luc-C_F Sequence file: RDB13972_A6Btb.seq
>D03116A6_A6Bt_3_Luc-C_F_H12_24.ab1 1 CTGAGGGGAT ACGTCGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG 61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG 121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG ATCGCCGTGG AATTCATGGA CGAGAAGGTG 181 TTCACCAAGG AGCTGGACCA GTGGATCGAG CAGCTGAACG AGTGCAAGCA GCTGTCCGAG 241 TCCCAGGTCA AGAGCCTCTG CGAGAAGGCT AAAGAAATCC TGACAAAAGA ATCCAACGTG 301 CAAGAGGTTC GATGTCCAGT TACTGTCTGT GGAGATGTGC ATGGGCAATT TCATGATCTC 361 ATGGAACTGT TTAGAATTGG TGGCAAATCA CCAGATACAA ATTACTTGTT TATGGGAGAT 421 TATGTTGACA GAGGATATTA TTCAGTTGAA ACAGTTACAC TGCTTGTAGC TCTTAAGGTT 481 CGTTACCGTG AACGCATCAC CATTCTTCGA GGGAATCATG AGAGCAGACA GATCACACAA 541 GTTTATGGTT TCTATGATGA ATGTTTAAGA AAATATGGAA ATGCAAATGT TTGGAAATAT 601 TTTACAGATC TTTTTGACTA TCTTCCTCTC ACTGCCTTGG TGGATGGGCA GATCTTCTGT 661 CTACATGGTG GTCTCTCGCC ATCTATAGAT ACACTGGATC ATATCAGAGC ACTTGATCGC 721 CTACAAGAAG TTCCCCATGA GGGTCCAATG TGTGACTTGC TGTGGTCAGA TCCAGATGAC 781 CGTGGTGGTT GGGGTATATC TCCTCGAGGA GCTGGTTACA CCTTTGGGCA AGATATTTCT 841 GAGACATTTA ATCATGCCAA TGGCCTCACG TTGGTGTCTA GAGCTCACCA GCTAGTGATG 901 GAGGGATATA ACTGGTGCCA GGACCGGAAT GTAGTAACGA TTTTTCAGTG CTTCCAAACT 961 ATTGTTATCG TTGTGGGTAA CCCAAAGCTG CAATCATGGG AACTTTGAAC GATTACTTCT 1021 TAAAAATACT TCTTTTCTTT GCAGTTTGAC CCCAAGCCAT CCTCGTTAGA GGCCGAGGCC 1081 ACATGGTACT CGATCGTAAC CCCCAAGACC TTACTTTCCC TTGGTTAAAA TGGT //
Primer: pAxCALNL_F1 Sequence file: RDB13972_A6Btc.seq
>D03116A6_A6Bt_3_pAxCALNL_F1_A08_02.ab1 1 AATAAGTATC TTATCATGTC TGCTCGAGCA TTAACCCTCA CTAAAGGGAA GCGGCCGCCC 61 GGGTCGACAT TACAGGAAGT AGTCTGGGGT ACGACGAGTA ACATGTGGCT CGCCTCTACG 121 AGGTGCTGGG TCAAACTGCA AGAAAGAGTA TTTTAGAGTA TCGTCAAGTT CCATGATTGC 181 AGCTTGGTTA CCACAACGAT AACAATAGTT TGGAGCACTG AAAATCGTTA CTACATTCCG 241 GTCCTGGCAC CAGTTATATC CCTCCATCAC TAGCTGGTGA GCTCTAGACA CCAACGTGAG 301 GCCATTGGCA TGATTAAATG TCTCAGAAAT ATCTTGCCCA AAGGTGTAAC CAGCTCCTCG 361 AGGAGATATA CCCCAACCAC CACGGTCATC TGGATCTGAC CACAGCAAGT CACACATTGG 421 ACCCTCATGG GGAACTTCTT GTAGGCGATC AAGTGCTCTG ATATGATCCA GTGTATCTAT 481 AGATGGCGAG AGACCACCAT GTAGACAGAA GATCTGCCCA TCCACCAAGG CAGTGAGAGG 541 AAGATAGTCA AAAAGATCTG TAAAATATTT CCAAACATTT GCATTTCCAT ATTTTCTTAA 601 ACATTCATCA TAGAAACCAT AAACTTGTGT GATCTGTCTG CTCTCATGAT TCCCTCGAAG 661 AATGGTGATG CGTTCACGGT AACGAACCTT AAGAGCTACA AGCAGTGTAA CTGTTTCAAC 721 TGAATAATAT CCTCTGTCAA CATAATCTCC CATAAACAAG TAATTTGTAT CTGGTGATTT 781 GCCACCAATT CTAAACAGTT CCATGAGATC ATGAAATTGC CCATGCACAT CTCCACAGAC 841 AGTAACTGGA CATCGAACCT CTTGCACGTT GGATTCTTTT GTCAGGATTT CTTTAGCCTT 901 CTCGCAGAGG CTCTTGACCT GGGACTCGGA CAGCTGCTTG CACTCGTTCA GCTGCTCGAT 961 CCACTGGTCC AGCTCCCTTG GGTGAACACC TTCTCGTCCA TGAATTTCCA CGGCGATCTT 1021 TCCGCCCTTC TTGGTCTTTA TGAAGATCTC TCTGATTTTT TCTTGCGTCG AGTTTTCCGG 1081 TAGACGTTCC GTTACCTCCG ATCGACAAAC ACAACTTCCC TTCCGCGCGG CAAACT //
Primer: SV40pro_F_V2 Sequence file: RDB13972_A6Btd.seq
>D03116A6_A6Bt_3_SV40pro_F_V2_B08_05.ab1 1 GAAGCCCTTT TTTGGAGCTA GGCTTTTGCA AAAGCTTGAT TCTTCTGACA CAACAGTCTC 61 GAACTTAAGG CTAGAGCCAC CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 121 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 181 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 241 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 301 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 361 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 421 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 481 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 541 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 601 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 661 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 721 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 781 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 841 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG GTTCGAAATG 901 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGATGGCCGC AATAAAATAT CTTTATTTTC 961 ATTACATCTG TGTGTTGGGT TTTTTTGTGT GAATCGATAG CGATAGAATC CGCGTATGGT 1021 GCACTCTCAG TACATCTGCT CTGATGCCGC ATAGTTTAAG GCAAGCCCCG ACACCCGCCG 1081 ACACCGCTGA CGCGCCTTGA ACAGGCATGG TCTGCCTCCC GGCCCAATCC CGCCCTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

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Other resources in our bank

human PP2A

External Database
human PP2A

firefly luciferase

Reference sequence