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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pClneoLuc-PP1A (#RDB13971)

Expression vector of luciferase-fused PP1A.

Clone info. Expression vector of luciferase-fused PP1A.
Comment Expression was confirmed by the depositor with luciferase activity. Known difference to PP1A (NM_002708.3): A to G at 799nt (Thr 226 Ala).
Vector backbone pCIneo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human PP1A cDNA firefly luciferase
Depositor|Developer Hata, Yutaka |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13971 pClneoLuc-PP1A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pClneoLuc-PP1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13971).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13971_A6Btp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13971_A6Bta.seq check
>D03116A5_A6Bt_2_CMV_Forward_E07_13.ab1
    1 GATCGCTGCG ATCGCGCGCC CCGTTGTACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG
   61 GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCA CTAGAAGCTT TATTGCGGTA
  121 GTTTATCACA GTTAAATTGC TAACGCAGTC AGTGCTTCTG ACACAACAGT CTCGAACTTA
  181 AGCTGCAGTG ACTCTCTTAA GGTAGCCTTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT
  241 AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA
  301 GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT
  361 TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA
  421 CGACTCACTA TAGGCTAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC
  481 CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT
  541 ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT
  601 ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA
  661 ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT
  721 TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG
  781 AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAATGGGT
  841 TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG
  901 ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC
  961 TCCCGGTTTT ATGATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAGA CATGCACTGA
 1021 TCATGACTTC TCTGAATCTA CTGATCTGCT TAATGTGTCG CTCTGGCCTC ATAGAACTGC
1081 CTGCTGAATC CGCATGCCGA GATCTAACTA TTGGCAATCC A
//
Primer: Luc-C_F
Sequence file: RDB13971_A6Btb.seq check
>D03116A5_A6Bt_2_Luc-C_F_G12_21.ab1
    1 TAGGCATCGT CGCCAGTCAG TAACAACCGC GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT
   61 GGACGAAGTA CCGAAAGGTC TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT
  121 CATAAAGGCC AAGAAGGGCG GAAAGATCGC CGTGGAATTC ATGTCCGACA GCGAGAAGCT
  181 CAACCTGGAC TCGATCATCG GGCGCCTGCT GGAAGTGCAG GGCTCGCGGC CTGGCAAGAA
  241 TGTACAGCTG ACAGAGAACG AGATCCGCGG TCTGTGCCTG AAATCTCGGG AGATTTTTCT
  301 GAGCCAGCCC ATTCTTCTGG AGCTGGAGGC ACCCCTCAAG ATCTGCGGTG ACATACACGG
  361 CCAGTACTAC GACCTTCTGC GACTATTTGA GTATGGCGGT TTCCCTCCCG AGAGCAACTA
  421 CCTCTTTCTG GGGGACTATG TGGACAGGGG CAAGCAGTCC TTGGAGACCA TCTGCCTGCT
  481 GCTGGCCTAT AAGATCAAGT ACCCCGAGAA CTTCTTCCTG CTCCGTGGGA ACCACGAGTG
  541 TGCCAGCATC AACCGCATCT ATGGTTTCTA CGATGAGTGC AAGAGACGCT ACAACATCAA
  601 ACTGTGGAAA ACCTTCACTG ACTGCTTCAA CTGCCTGCCC ATCGCGGCCA TAGTGGACGA
  661 AAAGATCTTC TGCTGCCACG GAGGCCTGTC CCCGGACCTG CAGTCTATGG AGCAGATTCG
  721 GCGGATCATG CGGCCCACAG ATGTGCCTGA CCAGGGCCTG CTGTGTGACC TGCTGTGGTC
  781 TGACCCTGAC AAGGACGTGC AGGGCTGGGG CGAGAACGAC CGTGGCGTCT CTTTTGCCTT
  841 TGGAGCCGAG GTGGTGGCCA AGTTCCTCCA CAAGCACGAC TTGGACCTCA TCTGCCGAGC
  901 ACACCAGGTG GTAGAAGACG GCTACGAGTT CTTTGCCAAG CGGCAGCTGG TGACACTTTT
  961 CTCAGCTCCC AACTACTGTG GCGAGTTTGA CATGCTGGGC GCCCATGATG AGTGTGGACG
 1021 AGACCCTCAT GTGCTCTTTC CAGATCTCAG CCCCGCCGAC AAGACCAGGG AGGTACCGGC
1081 AGTTCAGGTG GCCCTGGAAG CAG
//
Primer: pAxCALNL_F1
Sequence file: RDB13971_A6Btc.seq check
>D03116A5_A6Bt_2_pAxCALNL_F1_F07_16.ab1
    1 AACTTTCATG TTATCTTATC ATGTCTGCTC GAAGCATTAA CCCTCACTAA AGGGAAGCGG
   61 CCGCCCGGGT CGACTATTTC TTGGCTTTGG CGGAATTGCG GGGTGGGGTG ATGGGTCGGC
  121 CTCCAGGGTT CAGGCCACTG AACTGCCCGT ACTTCCCCTT GTTCTTGTCG GCGGGCTTGA
  181 GGATCTGGAA AGAGCACATG AGGGTCTCGT CCACACTCAT CATGGCGCCA GCATTGTCAA
  241 ACTCGCCACA GTAGTTGGGA GCTGAGAAAA GTGTCACCAG CTGCCGCTTG GCAAAGAACT
  301 CGTAGCCGTC TTCTACCACC TGGTGTGCTC GGCAGATGAG GTCCAAGTCG TGCTTGTGGA
  361 GGAACTTGGC CACCACCTCG GCTCCAAAGG CAAAAGAGAC GCCACGGTCG TTCTCGCCCC
  421 AGCCCTGCAC GTCCTTGTCA GGGTCAGACC ACAGCAGGTC ACACAGCAGG CCCTGGTCAG
  481 GCACATCTGT GGGCCGCATG ATCCGCCGAA TCTGCTCCAT AGACTGCAGG TCCGGGGACA
  541 GGCCTCCGTG GCAGCAGAAG ATCTTTTCGT CCACTATGGC CGCGATGGGC AGGCAGTTGA
  601 AGCAGTCAGT GAAGGTTTTC CACAGTTTGA TGTTGTAGCG TCTCTTGCAC TCATCGTAGA
  661 AACCATAGAT GCGGTTGATG CTGGCACACT CGTGGTTCCC ACGGAGCAGG AAGAAGTTCT
  721 CGGGGTACTT GATCTTATAG GCCAGCAGCA GGCAGATGGT CTCCAAGGAC TGCTTGCCCC
  781 TGTCCACATA GTCCCCCAGA AAGAGGTAGT TGCTCTCGGG AGGGAAACCG CCATACTCAA
  841 ATAGTCGCAG AAGGTCGTAG TACTGGCCGT GTATGTCACC GCAGATCTTG AGGGGTGCCT
  901 CCAGCTCCAG AAGAATGGGC TGGCTCAGAA AAATCTCCCG AGATTTCAGG CACAGACCGC
  961 GGATCTCGTT CTCTGTCAGC TGTACATTCT TGCCAGGCCG CGAGCCCTGC ACTTCCAGCA
 1021 GCGCCCGATG AATCGAGTCC AGGTTTGAGC TTTCTCGCTG TCGACATGAT TCACGGCGAT
1081 CTTCCGCCAT CATGGCTTTT ATGAAGGATT CTCCTCTGAA TTTTTTTTTT T
//
Primer: SV40pro_F_V2
Sequence file: RDB13971_A6Btd.seq check
>D03116A5_A6Bt_2_SV40pro_F_V2_G07_19.ab1
    1 GAACCCTTTT GGAGCTAGGC TTTTGCAAAA GCTTGATTCT TCTGACACAA CAGTCTCGAA
   61 CTTAAGGCTA GAGCCACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
  121 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
  181 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
  241 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
  301 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
  361 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
  421 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
  481 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
  541 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
  601 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
  661 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG
  721 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
  781 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC
  841 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC
  901 GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT
  961 ACATCTGTGT GTTGGGTTTT TTTGTGTGAA TCGATAGCGA TACGATCCGC GTATGGTGCA
 1021 CTCTCAGTAC CATCTGCTTC TGATGCCGCA TAGTTAAAGC AAGCCCCGGA CACCGACAAC
1081 ACCGCTGACG CGCCTTGACC GGCCTGATCC TGCTCCCGGG CCAATTCCGC GCTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]