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pEF-BOSneo-Myc-MINT deltaC

Expression vector of mouse SPEN/MINT cDNA

Catalog number RDB13476
Resource name pEF-BOSneo-Myc-MINT deltaC
Clone info. Expression vector of mouse SPEN/MINT cDNA. deltaC in Figure 1A of Kuroda, K. et al., Immunity 18 (2), 301-312, 2003.
This clone possibly contains nucleotide substitutions comparing with NM_019763.2. See comment of RDB13473 and RDB13474.
Vector backbone pEF-BOS Neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Mint cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Spen (NCBI Gene 56381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB13476 pEF-BOSneo-Myc-MINT deltaC DNA solution

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References and tips

Electronic file

Featured content

References

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13476_A5Hep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB13476_A5Hea.seq
>13476_13476_A5He_3_pAxEF_F1_E11_14.ab1
    1 CTCCTTTCAT CATTTCAGTG TCGTGAGGAT TCTCTAGCCC TCGACGCCAC CATGGAGCAA
   61 AAGCTCATTT CTGAAGAGGA CTTGGGAGAC CGAGACCTCC GCACGGATTA TAATGAGCCA
  121 GGAACTATTC CGAGTGCTGC CCGGGGACTG GACGAGACAG TTTCCATAGC GTCTCGTAGT
  181 AGAGAGGTTT CTGGGTTCAG AGGAAGTGCT GGAGGGCCTG CTTATGGTCC CCCACCATCA
  241 CTTCATGCAC GAGAAGGACG CTATGAGCGG AGACTCGATG GGGCTTCAGA TAACAGGGAG
  301 CGTGCTTATG AACATAGTGC CTATGGACAC CATGAACGGG GGACAGGAGC ATTTGATCGG
  361 ACAAGACATT ACGATCAGGA TTACTATAGA GACCCTCGAG AGCGGACTTT ACAACACGGG
  421 CTCTATTACA CTTCTCGGAG TCGAAGTCCA AACCGCTTTG ATGCTCATGA CCCCCGATAC
  481 GAACCTAGGG CCCGGGAGCA GTTTACACTG CCCAGTGTGG TACACAGGGA TATCTACAGG
  541 GACGACATTA CCCGGGAGGT ACGAGGCAGA AGGCCAGAGC GCAGTTACCA ACACAGCAGG
  601 AGTCGCTCAC CACATTCGTC CCAGTCTAGA AACCAGTCCC CGCAGAGGCT GGCTAGCCAA
  661 GCGTCCAGAC CCACCAGGTC CCCCAGCGGC AGCGGCTCCA GGAGTCGATC CTCCAGTAGT
  721 GACTCCATCA GCAGCAGCAG CAGCAGCAGC AACACTGACA GTAGCGACTC CAGCAGCACG
  781 GCCAGTGACG ACTCTCCGGC CCGCTCTGTT CAGTCGGCTG CAGTCCCCGC ACCCACTTCC
  841 CAGTTGCTTT CGTCCCTTGA AAAAGATGAG CCCCGGAAAA GTTTTGGGAT CAAAGTTCAG
  901 AATCTTTCCA GTACGCTCTA CAGACACAAG CCCTTAAAGA CGGCCTTTTT CCATGAGTTT
  961 AAGAAGTTTG GGCACGTGAC GTCAGTGCAG GATCCACGGG GCCTCCGAGG AGAGAATACG
 1021 TCTGTGTTCT TCCGGCAGCC AGAGACAGAA AGGCATTGAC GCATCCAGGA AGCTCTTCCT
1081 CCGGCATTGC
//
Primer: hGCSF-R RDB13476_A5Heb.seq
>13476_13476_A5He_3_hGCSF_R_D11_11.ab1
    1 CGGGGGAATG ATCTCTCAGG GTCCAGGTGG GGACCCTCAC TCTAGGATAT CATCTAGACG
   61 GATCCGCAAT TGGCCCCCAC CGCCTCCTGC AGCTCCCGCT CCAGTCTCTT CCTGGTCACT
  121 CTGGGCTGCT CCACAGGCTC CTTGGCCGGG GACCGGCTCT CGTGGTCGGT CATGGTGGCA
  181 TAAACACTCC TCACGTTCCG GCGCCTGCTC CTGCTGAGAG GCAAGCTAGG CTCAGGGTGC
  241 TCCGTGTCTC CCCCAGAGCT TTTCGACGCA GTCCTTGTAA TCTTCTCTGC CTCCATCTTT
  301 AGTTCCAGCA GCTTCTGTGC TTCTCCACGA GGAGAGCTGG ACCTCTTCAG CTTTTCCCGG
  361 TCTATCCTCT CGCTCTTCCT GGTGACAGGC TTCTCCACGA CGCTGGCGGC AGCCGCCTGG
  421 ACGGACGTCT TGGAACGCTT GCTCTTGTTA GTCTTCCTGT CTTTTGCAGC GACTGGAGGA
  481 TTGGCTTCTA CATCTTCAGA AGCGGGGGGC TGAGCTTCTG ATGCAACCCC AGCGTTCTGA
  541 GTGGCGTCAG GCTCAGTGCT TGGAGTTGTG TCTGGCTTCT CCACTTTCCC CTCTTCGGCT
  601 TCCTCGGACC TCTGAGTCAG TTTTGAAAGG GGCTGGGGAC TGCTAGGTTT GGGGTCTACA
  661 CTGCTTTCTA CCTGGGAAAC TGTGGTTCCG GGAGTCAGAG GTTCAGCATT CACACAAGGG
  721 ACGGCGTCAG TAGCTGCGCT TTCTTGAGGA GCTGAAGGTG CCAAGGCAGC GCTGTCCTGG
  781 CTATCCCGGC TGTCCTCTCC GGGGGGTACA TCTGACTGCC TGGGCTGCTC CACGGGTACA
  841 GACGCAGGCT CAGACACAAG CTTTGTTTCT TCCGAGACAG GAGCGGGCTC TGCAGGCTTC
  901 TCTCCTGCTG GTGAAGGAGC CTCCGCTGCC TCTTCAGCCT TCTCAGGGCG GAGGATGCTG
  961 GCTCTGGTGT CACGACGGTG ATGGTGACGG CAGGAGCCAG CTGAAACCAG GGGCCTTCCG
 1021 CTCAGCTCTT AGCTCTCTGC AGCAGGCTCT GGATTCTAGG ATGATCTCGG ACTCATATCC
1081 TTGCATTCCT CCAGGACCAT C
//
Primer: HSV TK pA_R RDB13476_A5Hec.seq
>13476_13476_A5He_3_HSV_TK_pA_R_G11_20.ab1
    1 CGACTCGGTC AGATGCGATA GAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA
   61 GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA
  121 ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA
  181 AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT
  241 CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT
  301 GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC
  361 GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA
  421 GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA
  481 GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA
  541 CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT
  601 CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC
  661 CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT
  721 CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT
  781 CAATGGCCGA TCCCATATTG GCTGCAGGGT CGCTCGGTGT TCGAGGCCAC ACGCGTCACC
  841 TTAATATGCG AAAGTGGACC TGGGACCGCG CCGCCCCCGA CTGCATCTGC GTGTTCGAAT
  901 TCGCCAATGA CAAGACGCTG GGCGGGGTTT GCTCGACATT GGGTGGAAAC ATTCCAGGCC
  961 CTGGGTGGAG AGGCTTTTTT GCCTTCCTCT TTGCCAAAAC CCACACTGCT CGAACATTGG
 1021 GTGGAAACAT TTCCAGGCCC TGGGTGAGAG CTTTTTTGGC TTCCTCCTGA AAACCCACCA
1081 CTGGCCTTCG GACTGACGGA AAGGGGGGGA ATGAATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956.

2019.02.05

GNP_filter3_RDBDEP_html_190131.pl