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pEF-BOSneo-Myc-MINT deltaC (#RDB13476)

Expression vector of mouse SPEN/MINT cDNA

Clone info. Expression vector of mouse SPEN/MINT cDNA. deltaC in Figure 1A of Kuroda, K. et al., Immunity 18 (2), 301-312, 2003.
Comment This clone possibly contains nucleotide substitutions comparing with NM_019763.2. See comment of RDB13473 and RDB13474.
Vector backbone pEF-BOS Neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Mint cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(Kuroda, K., Immunity, 18, 301-312, 2003)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13476 pEF-BOSneo-Myc-MINT deltaC DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneo-Myc-MINT deltaC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13476).

Reference section:

Kuroda, K., Han, H., Tani, S., Tanigaki, K., Tun, T., Furukawa, T., Taniguchi, Y., Kurooka, H., Hamada, Y., Toyokuni, S., Honjo, T., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13476_A5Hep1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 ()
Sequence file: RDB13476_A5Hee.seq check
>D02692A5_A5He_4_pAxEF_F1_B03_06_ABI24.ab1
    1 CATTTTTTTC TTCATTTCAG GTGTCGTGAG GATTCTCTAG CCCTCGACGC CACCATGGAG
   61 CAAAAGCTCA TTTCTGAAGA GGACTTGGGA GACCGAGACC TCCGCACGGA TTATAATGAG
  121 CCAGGAACTA TTCCGAGTGC TGCCCGGGGA CTGGACGAGA CAGTTTCCAT AGCGTCTCGT
  181 AGTAGAGAGG TTTCTGGGTT CAGAGGAAGT GCTGGAGGGC CTGCTTATGG TCCCCCACCA
  241 TCACTTCATG CACGAGAAGG ACGCTATGAG CGGAGACTCG ATGGGGCTTC AGATAACAGG
  301 GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACAGG AGCATTTGAT
  361 CGGACAAGAC ATTACGATCA GGATTACTAT AGAGACCCTC GAGAGCGGAC TTTACAACAC
  421 GGGCTCTATT ACACTTCTCG GAGTCGAAGT CCAAACCGCT TTGATGCTCA TGACCCCCGA
  481 TACGAACCTA GGGCCCGGGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC
  541 AGGGACGACA TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGCAGTTA CCAACACAGC
  601 AGGAGTCGCT CACCACATTC GTCCCAGTCT AGAAACCAGT CCCCGCAGAG GCTGGCTAGC
  661 CAAGCGTCCA GACCCACCAG GTCCCCCAGC GGCAGCGGCT CCAGGAGTCG ATCCTCCAGT
  721 AGTGACTCCA TCAGCAGCAG CAGCAGCAGC AGCAACACTG ACAGTAGCGA CTCCAGCAGC
  781 ACGGCCAGTG ACGACTCTCC GGCCCGCTCT GTTCAGTCGG CTGCAGTCCC CGCACCCACT
  841 TCCCAGTTGC TTTCGTCCCT TGAAAAAGAT GAGCCCCCGG AAAAGTTTTG GGATCAAAGT
  901 TCAGAATCTT CCAGTACGCT CTACAGACAC AAGCCTTTAA GACGCCCTTT TCCATGAGGT
  961 TTAAGAAGTT TGGGCAAAGG GTGACGTCCA GTGCCAGATC CCACGGGGCC CTCCCGAGGA
 1021 GGAGATACGG CCCTGTGTTC TTCCCGGCCA GCAAGGAAGA ACCAAGAAAA GCCAATTGAC
1081 GCATCAAGGG AAAGCCTTCT TCTTTCCGGC CAGTGCCCAG AAAT
//
Primer: hGCSF_R ()
Sequence file: RDB13476_A5Hef.seq check
>D02692A5_A5He_4_hGCSF_R_A03_03_ABI24.ab1
    1 CTTGGAACAT GATCTCTCAA GGGTCCCAGG TGGGGACCCT CACTCTAGGA TATCATCTAG
   61 ACGGATCCGC AATTGGCCCC CACCGCCTCC TGCAGCTCCC GCTCCAGTCT CTTCCTGGTC
  121 ACTCTGGGCT GCTCCACAGG CTCCTTGGCC GGGGACCGGC TCTCGTGGTC GGTCATGGTG
  181 GCATAAACAC TCCTCACGTT CCGGCGCCTG CTCCTGCTGA GAGGCAAGCT AGGCTCAGGG
  241 TGCTCCGTGT CTCCCCCAGA GCTTTTCGAC GCAGTCCTTG TAATCTTCTC TGCCTCCATC
  301 TTTAGTTCCA GCAGCTTCTG TGCTTCTCCA CGAGGAGAGC TGGACCTCTT CAGCTTTTCC
  361 CGGTCTATCC TCTCGCTCTT CCTGGTGACA GGCTTCTCCA CGACGCTGGC GGCAGCCGCC
  421 TGGACGGACG TCTTGGAACG CTTGCTCTTG TTAGTCTTCC TGTCTTTTGC AGCGACTGGA
  481 GGATTGGCTT CTACATCTTC AGAAGCGGGG GGCTGAGCTT CTGATGCAAC CCCAGCGTTC
  541 TGAGTGGCGT CAGGCTCAGT GCTTGGAGTT GTGTCTGGCT TCTCCACTTT CCCCTCTTCG
  601 GCTTCCTCGG ACCTCTGAGT CAGTTTTGAA AGGGGCTGGG GACTGCTAGG TTTGGGGTCT
  661 ACACTGCTTT CTACCTGGGA AACTGTGGTT CCGGGAGTCA GAGGTTCAGC ATTCACACAA
  721 GGGACGGCGT CAGTAGCTGC GCTTTCTTGA GGAGCTGAAG GTGCCAAGGC AGCGCTGTCC
  781 TGGCTATCCC GGCTGTCCTC TCCGGGGGGG GTACATCTGA CTGCCTGGGC TGCTCCACGG
  841 GGTACAGACG CAGGCTCAGA CACAAGCTTT GGTTTCTTCC GAGACAGGAG CGGGCTCTGC
  901 AGGCTTTCTC TCCTGCTGGT GAAGGAGCCT CCGCTGCCTC CTTCAGCCTT CTCAAGGGCG
  961 GAGGATGCTG GCTCTGGTGT CACGACCGGT GAATGGTGAC GGCCAGAAAG CCCCAGCCTG
 1021 AAAACAGGGG GCTTTCGGGC TCCGGCTTCT TTAGTCTCTG CAGCCAGCTC TGGAAGTCCT
1081 GGATGAGTCT CCCGGCTCTT TCCTGCCTTT C
//
Primer: HSV_TK_pA_R ()
Sequence file: RDB13476_A5Heg.seq check
>D02692A5_A5He_4_HSV_TK_pA_R_C03_09_ABI24.ab1
    1 CGGAACTCGT CAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT
   61 AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG
  121 CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG
  181 AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA
  241 GATCCTCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC
  301 CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG
  361 CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT
  421 GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG
  481 ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA
  541 CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG
  601 CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC
  661 GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC
  721 AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT
  781 GTTCAATGGC CGATCCCATA TTGGCTGCAG GGTCGCTCGG TGTTCGAGGC CACACGCGTC
  841 ACCTTAATAT GCGAAGTGGA CCTGGGACCG CGCCGCCCCG ACTGCATCTG CGTGTTCGAA
  901 TTCGCCAATG ACAAGACGCT GGGGCGGGGT TTGCTCGACA TTGGGTGGGA AACATTCCAG
  961 GCCCTGGGTG GAGAGGCTTT TTTTGCTTCC CTCTTGCAAA ACCACCACTG CTCGACATTT
 1021 GGTGGAAACA TTCCAGGCCT TGGGTTGGAG AGCTTTTTTT GCTTCCTCAT GAAACGACAC
1081 TGCTTCGACT GGGCGAAAGG GGGGCAA
//
Primer: EF1a-N_R ()
Sequence file: RDB13476_A5Heh.seq check
>D02692A5_A5He_4_EF1a-N_R_E03_15_ABI24.ab1
    1 GGGTTCATGA TGGGGCGATG TGCGCTCTGC CCACTGACGG GCACCGGAGC CAATTCCCAC
   61 TCCTTTCAAG ACCTAGAAGG TCCATTAGCT GCAAAGATTC CTCTCTGCTT AAAACTTTAT
  121 CCATCTTTGC AAAGCTTTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC
  181 TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG
  241 CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG
  301 GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG
  361 GAGGGAATTA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
  421 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
  481 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
  541 CCTGTCGTGC CAGTCGAGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC
  601 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT
  661 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG
  721 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC
  781 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA
  841 CCCGCTGACG CGCCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
  901 GACCGTCTCC GGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTTCATCACC GAAACGCGCG
  961 AGACGAAAAG GGCCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG AATAATAAAT
 1021 GGTTTTCTTA GACGGTCAGG TGGCCACTTT TTCGGGGGAA ATGTGCGCGG AACCCCTAAT
1081 TTGATTAATT TTTTCTAAAT AACATTCAAA AATAATTGGT AT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]