Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1/ENGase/FLAG-His (#RDB13417)

Expression clone of mouse ENGase.

Clone info. Expression clone of mouse ENGase.
Comment V5 tag in the original vector was replaced by a FLAG tag.
Vector backbone pcDNA3.1/V5-HIS (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Mouse ENGase
Depositor|Developer suzuki, tadashi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13417 pcDNA3.1/ENGase/FLAG-His DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1/ENGase/FLAG-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13417).

Reference section:

Huang, C., Harada, Y., Hosomi, A., Masahara-Negishi, Y., Seino, J., Fujihira, H., Funakoshi, Y., Suzuki, T., Dohmae, N., Suzuki, T., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13417_A5E1p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13417_A5E1a.seq check
>13417_13417_2_CMV_Forward_A08_02.ab1
    1 CGTTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCACTA
  181 GTCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CAGTCATGGA GACCTCGTCG
  241 GTGCTGACCC GGGGAGCTGC AAGGCAGCGG AGCCCCGCGG CCCCGGAGAA GCAGGCGCGG
  301 GACCAGACGG AGCGGAGGCC GGGGCGGCGG AGGCAGGGGC GGAGGATCAA CGAGGATCAA
  361 GAGGAGGAAG CAGTCTTTAG AGAGGTGGTC AGTTTCACCC CGGATCCTTT ACCAGCTAGA
  421 TATTATGACA AGGATACTAC CAGACCTATC AGCTTTTACC TGTCGACGCT GGAGGAGCTC
  481 CTGGCCTGGA CCCCGCTCAT GGAGGATGGC TTCAATGTAG CCCTGGAACC CCTGGTGTGT
  541 CGCCGGCCTC CACTCAGCAG CCCCAGGCCC CGGACCCTGC TGTGCCACGA CATGATGGGT
  601 GGTTACCTGG AGGACAGGTT CATTCAGGGC TCAGAGGTGC AGAACCCCTA TTCCTTCTAC
  661 CACTGGCAGT ATATCGACAT CTTTGTGTAC TTCAGCCACC ACACGGTCAC CATCCCCCCA
  721 GTGTGCTGGA CCAATGCCGC ACACAGGCAC GGGGTCTGCG TGCTGGGGAC CTTCATCACA
  781 GAGTGGCAAG AGGGAGGCAG GCTCTGCGAA GCCTTCCTGG CCGGGGATGA GCCCTCCTTC
  841 CAGGCCGTGG CCGATCGGCT GGTCCAGATT GCTCAGTTCT TTCGTTTTGA CGGCTGGCTC
  901 ATCAACATCG AGAACTCCCT GACTCCAGCT GCCGTGAGGA ACACACCTTT TATTTCTCCA
  961 GTACCTTACC GCACAGCTCC ATCAGCAGGT CCCCCGGAGG TCTGGTGCTG TGGTACGACA
 1021 GTGTGGTACA GAGCGGGGCA GCTCAGTGGC AGGATGAACT TGCATGATCA GGAACCCGGG
1081 TCCTTCTTTG ACTCCCTGCC GGAAATGGCC CATC
//
Primer: BGH_rev
Sequence file: RDB13417_A5E1b.seq check
>13417-13417-_2_BGH-rev_D03_12.ab1
    1 GGCTTTCTTA ATGGTGATGG TGATGATGAC CGGTACGCTT ATCGTCGTCA TCCTTGTAAT
   61 CCTGAGGGAC CGAGAAGAGC AGGACCGCCT TTCCCCACTC AGCCTGGGGC ACCAGGAAAC
  121 CCTCCCTGGG GACAGGCTCC ACCAGGAACT CCACACGACC ATCCTGCCCA AATCCAGCAG
  181 CCTCCACAGC CAAGTCCACC ACACGGTACT GGTTGGCGAA AGCCAGGCCC AGGAACGTGG
  241 GCTTCTCTGT CTCTGGGGAC TCTGCCACAG AGCTGCTGCT TCCTGTCCGT TTCCAGCAGT
  301 GAATGCGGAA GCAGCGGGCT CGGAGCAGCA GGTAGGACCA GTGCAGGGTG CAGCTGAGGA
  361 GGAGCCGGGT AGGGAGGCCC TCAGACCCGG TGATCAGAGG AAGCCATCGT ATCTGAGAAA
  421 TGGTGACATT CTGCACCCGG GGCAGAGGGG CCAGAAGGCT GCTGGCATCA ACCACCTGGA
  481 TCTCTCCAAG GCGACAAATG AAGCTCTCCT CCTCCCGGCT GCCTGGCGGC CGTGAGAAGC
  541 TCACCAAGAG GTCCTGGAGC AGGCACCCAC GAAGGTTTAC CTCATAGCAG CGTTGAATCC
  601 AGCCCCCACT GAGCTGCTGG CCACAACTGC TGGCCCATCT GGCCAGCCTG GTGGGGGGTA
  661 CCCGGAGGGG TCTGGGGCTG TGCCGTGAGC CAGTTTCATT TAACACCAAC ATGCCACCCA
  721 CATGACAGCT GCTGGCATCC CCTGTTGTGA GCTCCAAAGC CACCTGGACA TCTGTTGAGC
  781 CTTCAAACTT GTACACCATG GACAGGAAAA CCTTGGGTGG CACTGGGATG TGCAGAGAGA
  841 ACAGCCTCAC AGCTACACTG TCAACCTCAG GCGGGATCAG GCCCCGGAGC AGCAGAGAGC
  901 TGCCCCCGTG CCAGGCATCA GTCAGGCAGC AGTGCGTCTT CACCCAGCCC CGACTGTCAC
  961 CAGCCAGCTT ATGTTCACCA AAGACGGGCT GTGTCTCCCT GGGCGCTAGG GTGGTACCAG
 1021 GGCCCCCACT GCTTTGCTCT TTGCCGTAGC AGACTCTTTC GAGTACCCAG GGCCCAGCAG
1081 AAAGGAGTTG ACGATGCCAG AAGCAGAATA CTGTGTGTGG GAAGGAGGCC GGCT
//
Primer: SV40pro_F
Sequence file: RDB13417_A5E1c.seq check
>13417-13417_2_SV40pro_F_E03_15.ab1
    1 CCTGTCTGGG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTTCGCG AATGACCGAC CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATTTC CACCGCCGCT TCTATGGAAC GTGGGCTTCG
 1021 ATCGTTTTCA GGGAACGCGC TGAATGATTC TCCAGCGCGG GATCTCATGC CTTGAGTCAT
1081 CGCCACTCAC CTGTATGCAG CTATATGTTA CAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]