Sequencing and PCR primers for primer information.
Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
| Catalog number | RDB13355 |
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| Resource name | pONL-N1_Golgi |
| Clone info. | Expression vector of the Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI. |
| Vector backbone | pEGFP-N1 (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r (E. coli) |
| Gene/insert name | human beta 1,4-galactosyltransferase cDNA |
| Depositor | Okada, Yasushi | Takai, Akira | |
| Other clones in our bank | human B4GALT1 (NCBI Gene 2683) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13355 | pONL-N1_Golgi | DNA solution |
Electronic file
Featured content
| Featured content | Nano-lantern (English text) |
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| Featured content | Nano-lantern (Japanese text) |
| Featured content | Organelle marker (English text) |
References
| original | Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13355_A5E8p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB13355_A5E8a.seq |
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>13355_13355_A5E8_3_CMV_Forward_H10_22.ab1 1 AGTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCATG AGGCTTCGGG AGCCGCTCCT 121 GAGCGGCAGC GCCGCGATGC CAGGCGCGTC CCTACAGCGG GCCTGCCGCC TGCTCGTGGC 181 CGTCTGCGCT CTGCACCTTG GCGTCACCCT CGTTTACTAC CTGGCTGGCC GCGACCTGAG 241 CCGCCTGCCC CAACTGGTCG GAGTCTCCAC ACCGCTGCAG GGCGGCTCGA ACAGTGCCGC 301 CGCCATCGGG CAGTCCTCCG GGGAGCTCCG GACCGGAGGG GCCAAGGATC CACCGGTCGC 361 CACCATGGTG AGCGTGATCA AGCCCGAGAT GAAGATGAGG TACTACATGG ACGGCTCCGT 421 CAATGGGCAT GAGTTCACAA TCGAGGGTGA GGGCACAGGC AGACCTTACG AGGGACATCA 481 GGAGATGACA CTGCGCGTCA CAATGGCCGA GGGCGGGCCA ATGCCTTTCG CCTTCGACCT 541 GGTGTCCCAC GTGTTCTGTT ACGGCCACAG AGTTTTTACT AAATATCCAG AAGAGATCCC 601 AGACTATTTC AAGCAGGCCT TTCCTGAGGG CCTGTCCTGG GAGAGGTCCC TGGAGTTCGA 661 GGACGGCGGC TCCGCCTCCG TGAGCGCCCA CATCAGCCTG AGGGGCAACA CCTTCTACCA 721 CAAGTCCAAG TTCACCGGCG TGAACTTCCC CGCCGACGGC CCCATCATGC AGAACCAGAG 781 CGTGGACTGG GAGCCCTCCA CCGAGAAGAT CACCGCCAGC GACGGCGTGC TGAAAGGGCG 841 ACGTGACCAT GTACCTGAAG CTGGAGGGCG GCGGGCAACC CACAAGTGCC AGATGAAGAC 901 CACCCTACAA GGCCGCCCAA GGAGATCCCT GGAGATGCCC GGGCGACCAC TACCATCGGG 961 CCACAGCTGG TGAGAAGACC GGAAGGCCAC CATCACCGGA GCAGTGAAGG AACGCGTTGC 1021 CCACCTCCGT ACCAAGTGTA CGAACCCCGA GCATCGCAAC GCATGATCAC TGAGCCCTCA 1081 TTTGGAATG // |
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| Primer: ME-1290Rv | RDB13355_A5E8b.seq |
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>13355_13355_A5E8_3_ME_1290Rv_H06_24.ab1 1 ATGCCATTCA TTTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG 61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCCTACTGCT 121 CGTTCTTCAG CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT 181 CCTCCTGGAG GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG 241 CTCCCTCGAC AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC AGCTTAGGCA 301 GATCGTCGCT GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT 361 TGCCTCCCTT AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC 421 CCTTCTCCTT GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA 481 TCTTGCTTGG CAACAGGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT 541 CTTCGCTCTT GATCAGGGCC AGCTCCTCCT CGATGTCAGG CCACCCCATC CAGGACTCGA 601 TCACGTCCAC GACACTCTCC ATATGGACGA TGGCCTTGAT CCTGTCTTGG TGCTCGTAGG 661 CGTAGTGAAA GGCCAGAGCG CTCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA 721 GGTTCAGCAG CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC 781 CATTCCCGCT CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA 841 CGGGCTCGAT GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGTAGCG TTACCATGCA 901 GAAAAATCAC GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAGGAGTCCA 961 GCACGTTCAT TTGCTTGCAG CGAGCCCACC ACTGAGGCCC AGTGATCATG CGTTTGCGTT 1021 GCTCGGGGTC GTACACCTTT GGTACGGAGT GGGCCACGGC GTCTCACTGC TCGATGATGG 1081 TGCCTCGGTC TCCTTCAACA GGCTGATGGA ACCGAGTGTT AGGTTGGCAT TC // |
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| Primer: SV40pro_F_V2 | RDB13355_A5E8c.seq |
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>13355_13355_A5E8_SV40pro_F_V2_C03_03_ABI08.ab1 1 CTACGACCCT TTTTTTGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG 61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG 601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG GCCTTCTATG AAAGGTTGGG 961 GCTTCGGAAT CGTTTTTCCG GGACGCCGGC TGGGATGAAT CCTCCAGCGC GGGGAATCTC 1021 ATGCTGGGAG TTCTTCGCCC AACCCTTAGG GGGAGGCTAC TGAAACACCG GAAGGGAGAA 1081 CAAATACCGG AAGAATCCCG CTGCTATGAA CGGCCAATAA AAGACCGATA ACGCACACGG 1141 GGTGTTGGGG TCCGATTTTG GTTTCTCAAT A // |
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Please visit Sequencing and PCR primers for primer information.
References
2019.04.09
GNP_filter3_RDBDEP_html_190327.pl