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pUC-T7-HCV-PABP-HA (#RDB13281)

HCV IRES-dependent expression vector of human PABP

Clone info. HCV IRES-dependent expression vector of human PABP.
Comment Expression was confirmed by depositor with Western blotting.
Vector backbone pUC-T7-HCV (Plasmid)
Size of vector backbone 3.4 kb
Selectable markers Amp^r
Gene/insert name Human PABP cDNA
Depositor|Developer Imataka, Hiroaki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
提供案内 (日本国内) [open/close]

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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13281 pUC-T7-HCV-PABP-HA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC-T7-HCV-PABP-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13281).

Reference section:

Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13281_A7Drp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB13281_A7Dra.seq check
>13281_13281_A7Dr_Reverse2_A12_03_ABI24.ab1
    1 CATGCATGCA TTACGCCAGC TTTATACGAC TCACTATAGG GAGACCGGCA GATCTGCCAG
   61 CCCCCGATTG GGGGCGACAC TCCACCATAG ATCACTCCCC TGTGAGGAAC TACTGTCTTC
  121 ACGCAGAAAG CGTCTAGCCA TGGCGTTAGT ATGAGTGTCG TGCAGCCTCC AGGACCCCCC
  181 CTCCCGGGAG AGCCATAGTG GTCTGCGGAA CCGGTGAGTA CACCGGAATT GCCAGGACGA
  241 CCGGGTCCTT TCTTGGATCA ACCCGCTCAA TGCCTGGAGA TTTGGGCGTG CCCCCGCGAG
  301 ACTGCTAGCC GAGTAGTGTT GGGTCGCGAA AGGCCTTGTG GTACTGCCTG ATAGGGTGCT
  361 TGCGAGTGCC CCGGGAGGTC TCGTAGACCG TGCACCATGA GCACGAATCC TAAACCTCAA
  421 AGAAAAACCG GATCCGAATT CCATATGAGA TCTGCTAGCG TCGACGATAT CCTCGAGTCT
  481 AGCATGAACC CCAGTGCCCC CAGCTACCCC ATGGCCTCGC TCTACGTGGG GGACCTCCAC
  541 CCCGACGTGA CCGAGGCGAT GCTCTACGAG AAGTTCAGCC CGGCCGGGCC CATCCTCTCC
  601 ATCCGGGTCT GCAGGGACAT GATCACCCGC CGCTCCTTGG GCTACGCGTA TGTGAACTTC
  661 CAGCAGCCGG CGGACGCGGA GCGTGCTTTG GACACCATGA ATTTTGATGT TATAAAGGGC
  721 AAGCCAGTAC GCATCATGTG GTCTCAGCGT GATCCATCAC TTCGCAAAAG TGGAGTAGGC
  781 AACATATTCA TTAAAAATCT GGACAAATCC ATTGATAATA AAGCACTGTA TGATACATTT
  841 TCTGCTTTTG GTAACATCCT TTCATGTAAA GGTGGTTTGT GATGAAAATG GTTCCAAAGG
  901 GCTACGGATT TGTACACTTT GAGACGCAGA AAGCAGCTGA AAGAGCTATT GAAAAAAATG
  961 AATGGAAATG CTCCTAAATG ATCGCAAAGT ATTTGTTGGG ACGATTTAAA GTCTCGTAAG
 1021 AACGAGAAGC TGAACTGCAG CTAGGCAAAA AGAATTCACC AATGGTTACA TCCAGCATTT
 1081 TGCAGAAGAC TGGATGAATG AAGCGCGTTA ACGATCTTCT TTGGGGCCTT GCCGTTAAAG
1141 ACG
//
Primer: M13_-40
Sequence file: RDB13281_A7Drb.seq check
>13281_13281_A7Dr_M13(-40)_B12_06_ABI24.ab1
    1 CCGGTCAGGA TAGCGGCCGC GGATGATCCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTCTA GATGCATGCT CGAGCGGCCG CCAGTGTGAT GGATATCTGC
  121 AGAATTCCAG CACACTGGCG GCCGTTACTA GTGGATCCTT AGCCCGCATA GTCAGGAACA
  181 TCGTATGGGT AAACAGTTGG AACACCGGTG GCACTGTTAA CTGCTTTCTG GGCAGCCTCT
  241 TTAGCTTGGT GGGCTTGTAG TACAGCTACA GCTTCATCAA CCTTAGAACG GAGTGACTCT
  301 GGAGACTCGA GCATATGAAG AAGTTCTGAA TTATCAATCT CCAACAACAT GCCAGTGATT
  361 TTACCAGCAA GAGTAGGGTG CATGGCTTGA ATAAGAGGAA ACAGCCGTTC ACCCAACATT
  421 TGCTTTTGCT CTTGAGGAGG GGCAGATGCC AACATGGAAG CAGTCAAAGG TTCCTGACCT
  481 TGTACATGAA CAGCAGGCTG TTGCATTGTA ACTTGTGGCT GTGCATTAAG ATGTTGCTGA
  541 GGATTGCGAA CTCCTGCAGC ATATTTATAC TGTGGAACGG TGCGGACAGC AGGAGTAGCT
  601 GCAGCGGCTG CAGCTGCAGG ACGTGGACCC ATTGTCTGTG TTGATGTGTT AGCAACACGC
  661 TGTGTTGACA TGACTCGTGG AACCTGTGAA GAAGCTGGTC TCATAGTACT AAATGGTGGT
  721 CTAGGAGCAG CTGGGCGGAT AGCACCGGGC ATATTTTGGA ATGGATGAGG TCTGGCACCC
  781 TGAGCAGTCC AGCGAGGACT TGGTCTTAGT TGAGCAACTT GGCTAGGAGG ATAGTATGCA
  841 GCACGGTTCT GAGTCTGTGG GATAGCTGCC ATGAAGTAAC CTGAAGGAGG TGCTGGCTGG
  901 TAGGGGTTGA TTACAGGGTT GGGAACAGCT CGTACACTTG CCATTCTCTG CATATACTGG
  961 TTTAGTGAGG TGAGCCTGGC GCTCTTCTTT GCGCTGAGCT AAAGCTACAT ACAATGGGCT
 1021 TTGTGGCCAC AATTCTACCG ATCATTTCTG TAACTGCTTT AGTGGCTCCT CTGGGGAGAG
 1081 AAAACATACA ATCGAAATCG TTGGCCTTGC GACCACCCTC CAATTCAATA ACCGTTTTGC
1141 ACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.