HCV IRES-dependent expression vector of human PABP
Clone info. | HCV IRES-dependent expression vector of human PABP. |
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Comment | Expression was confirmed by depositor with Western blotting. |
Vector backbone | pUC-T7-HCV (Plasmid) |
Size of vector backbone | 3.4 kb |
Selectable markers | Amp^r |
Gene/insert name | Human PABP cDNA |
Depositor|Developer | Imataka, Hiroaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13281 | pUC-T7-HCV-PABP-HA | DNA solution |
Materials & Methods section:
The pUC-T7-HCV-PABP-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13281). |
Reference section:
Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13281_A7Drp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: Reverse2 Sequence file: RDB13281_A7Dra.seq |
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>13281_13281_A7Dr_Reverse2_A12_03_ABI24.ab1 1 CATGCATGCA TTACGCCAGC TTTATACGAC TCACTATAGG GAGACCGGCA GATCTGCCAG 61 CCCCCGATTG GGGGCGACAC TCCACCATAG ATCACTCCCC TGTGAGGAAC TACTGTCTTC 121 ACGCAGAAAG CGTCTAGCCA TGGCGTTAGT ATGAGTGTCG TGCAGCCTCC AGGACCCCCC 181 CTCCCGGGAG AGCCATAGTG GTCTGCGGAA CCGGTGAGTA CACCGGAATT GCCAGGACGA 241 CCGGGTCCTT TCTTGGATCA ACCCGCTCAA TGCCTGGAGA TTTGGGCGTG CCCCCGCGAG 301 ACTGCTAGCC GAGTAGTGTT GGGTCGCGAA AGGCCTTGTG GTACTGCCTG ATAGGGTGCT 361 TGCGAGTGCC CCGGGAGGTC TCGTAGACCG TGCACCATGA GCACGAATCC TAAACCTCAA 421 AGAAAAACCG GATCCGAATT CCATATGAGA TCTGCTAGCG TCGACGATAT CCTCGAGTCT 481 AGCATGAACC CCAGTGCCCC CAGCTACCCC ATGGCCTCGC TCTACGTGGG GGACCTCCAC 541 CCCGACGTGA CCGAGGCGAT GCTCTACGAG AAGTTCAGCC CGGCCGGGCC CATCCTCTCC 601 ATCCGGGTCT GCAGGGACAT GATCACCCGC CGCTCCTTGG GCTACGCGTA TGTGAACTTC 661 CAGCAGCCGG CGGACGCGGA GCGTGCTTTG GACACCATGA ATTTTGATGT TATAAAGGGC 721 AAGCCAGTAC GCATCATGTG GTCTCAGCGT GATCCATCAC TTCGCAAAAG TGGAGTAGGC 781 AACATATTCA TTAAAAATCT GGACAAATCC ATTGATAATA AAGCACTGTA TGATACATTT 841 TCTGCTTTTG GTAACATCCT TTCATGTAAA GGTGGTTTGT GATGAAAATG GTTCCAAAGG 901 GCTACGGATT TGTACACTTT GAGACGCAGA AAGCAGCTGA AAGAGCTATT GAAAAAAATG 961 AATGGAAATG CTCCTAAATG ATCGCAAAGT ATTTGTTGGG ACGATTTAAA GTCTCGTAAG 1021 AACGAGAAGC TGAACTGCAG CTAGGCAAAA AGAATTCACC AATGGTTACA TCCAGCATTT 1081 TGCAGAAGAC TGGATGAATG AAGCGCGTTA ACGATCTTCT TTGGGGCCTT GCCGTTAAAG 1141 ACG // |
Primer: M13_-40 Sequence file: RDB13281_A7Drb.seq |
>13281_13281_A7Dr_M13(-40)_B12_06_ABI24.ab1 1 CCGGTCAGGA TAGCGGCCGC GGATGATCCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTCTA GATGCATGCT CGAGCGGCCG CCAGTGTGAT GGATATCTGC 121 AGAATTCCAG CACACTGGCG GCCGTTACTA GTGGATCCTT AGCCCGCATA GTCAGGAACA 181 TCGTATGGGT AAACAGTTGG AACACCGGTG GCACTGTTAA CTGCTTTCTG GGCAGCCTCT 241 TTAGCTTGGT GGGCTTGTAG TACAGCTACA GCTTCATCAA CCTTAGAACG GAGTGACTCT 301 GGAGACTCGA GCATATGAAG AAGTTCTGAA TTATCAATCT CCAACAACAT GCCAGTGATT 361 TTACCAGCAA GAGTAGGGTG CATGGCTTGA ATAAGAGGAA ACAGCCGTTC ACCCAACATT 421 TGCTTTTGCT CTTGAGGAGG GGCAGATGCC AACATGGAAG CAGTCAAAGG TTCCTGACCT 481 TGTACATGAA CAGCAGGCTG TTGCATTGTA ACTTGTGGCT GTGCATTAAG ATGTTGCTGA 541 GGATTGCGAA CTCCTGCAGC ATATTTATAC TGTGGAACGG TGCGGACAGC AGGAGTAGCT 601 GCAGCGGCTG CAGCTGCAGG ACGTGGACCC ATTGTCTGTG TTGATGTGTT AGCAACACGC 661 TGTGTTGACA TGACTCGTGG AACCTGTGAA GAAGCTGGTC TCATAGTACT AAATGGTGGT 721 CTAGGAGCAG CTGGGCGGAT AGCACCGGGC ATATTTTGGA ATGGATGAGG TCTGGCACCC 781 TGAGCAGTCC AGCGAGGACT TGGTCTTAGT TGAGCAACTT GGCTAGGAGG ATAGTATGCA 841 GCACGGTTCT GAGTCTGTGG GATAGCTGCC ATGAAGTAAC CTGAAGGAGG TGCTGGCTGG 901 TAGGGGTTGA TTACAGGGTT GGGAACAGCT CGTACACTTG CCATTCTCTG CATATACTGG 961 TTTAGTGAGG TGAGCCTGGC GCTCTTCTTT GCGCTGAGCT AAAGCTACAT ACAATGGGCT 1021 TTGTGGCCAC AATTCTACCG ATCATTTCTG TAACTGCTTT AGTGGCTCCT CTGGGGAGAG 1081 AAAACATACA ATCGAAATCG TTGGCCTTGC GACCACCCTC CAATTCAATA ACCGTTTTGC 1141 ACT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP] |
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reference | Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838. |
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