Resource data sheet
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pGEX6P-eRF1

Expression vector of human eRF1

Catalog number RDB13269
Resource name pGEX6P-eRF1
Clone info. Expression vector of human eRF1.
Expression was confirmed by depositor with Western blot and CBB staining.
Vector backbone pGEX6P (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human eRF1 cDNA
Depositor Imataka, Hiroaki |
 
Other clones in our bank human ETF1 (NCBI Gene 2107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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RDB13269 pGEX6P-eRF1 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13269_A7Drp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 RDB13269_A7Dra.seq
>13269_13269_A7Dr_pGEX-F2_A10_01_ABI24.ab1
    1 GCTACTAGTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC
   61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
  121 CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCCC GGAATTCATG
  181 GCGGACGACC CCAGTGCTGC CGACAGGAAC GTGGAGATCT GGAAGATCAA GAAGCTCATT
  241 AAGAGCTTGG AGGCGGCCCG CGGCAATGGC ACCAGCATGA TATCATTGAT CATTCCTCCC
  301 AAAGACCAGA TTTCACGAGT GGCAAAAATG TTAGCGGATG AGTTTGGAAC TGCATCTAAC
  361 ATTAAGTCAC GAGTAAACCG CCTTTCAGTC CTGGGAGCCA TTACATCTGT ACAACAAAGA
  421 CTCAAACTTT ATAACAAAGT ACCTCCAAAT GGTCTGGTTG TATACTGTGG AACAATTGTA
  481 ACAGAAGAAG GAAAGGAAAA GAAAGTCAAC ATTGACTTTG AACCTTTCAA ACCAATTAAT
  541 ACGTCATTGT ATTTGTGTGA CAACAAATTC CATACAGAGG CTCTTACAGC ACTACTTTCA
  601 GATGATAGCA AGTTTGGATT CATTGTAATA GATGGTAGTG GTGCACTTTT TGGCACACTC
  661 CAAGGAAACA CAAGAGAAGT CCTGCACAAA TTCACTGTGG ATCTCCCAAA GAAACACGGT
  721 AGAGGAGGTC AGTCAGCCTT GCGTTTTGCC CGTTTAAGAA TGGAAAAGCG ACATAACTAT
  781 GTTCGGAAAG TAGCAGAGAC TGCTGTGCAG CTGTTTATTT CTGGGGACAA AGTGAATGTG
  841 GCTGGTCTAG TTTTAGCTGG ATCCGCTGAC TTTAAAACTG AACTAAGTCA ATCTGATATG
  901 TTTGATCAGA GGTTACAATC AAAAGTTTTA AAATTAGTTG ATATATCCTA TGGTGGTGAA
  961 AATGGATTCA ACCCAAGCTA TTTGAGTTAT CTACTGAAGT CCTCTCTACG TGAAATTCAT
 1021 TCAAGAGAGA AATTATAGGA CGATACTTTG AATGAAATCA GCCAGGGACA CGGGCAAGTT
1081 ACCTGGTTTT GCCGATTGGA AGGATACACT AAAGGCCTTT GGGAAAATTG GGGCAC
//
Primer: pGEX-R RDB13269_A7Drb.seq
>13269_13269_A7Dr_pGEX-R_B10_04_ABI24.ab1
    1 TCAATAAAGG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGCGGCC GCTCGAGCTA
   61 GTAGTCATCA AGGTCAAAAA ATTCATCGTC TCCTCCTTGG TATTCCATTC CCTGGAAATC
  121 TACTCGGTAC CGCAAGATAC CTCCAATTCC ACCAAATCCT TTCACAAACT GAGACCCTTC
  181 TTGTGATTTA TCTGTGACAA TTTCCAACGT AGCTCCAAAT TTTTTATAGT TGTTAGCAAA
  241 CCATTCCAAC AGGGGCATGC TCTCGATAAG CTCATGTTCC TGTCCGGTCT CTTTGTCTGT
  301 GAAATGAGAT TTATCCTTTT CTTGCTCTGG AGTTAGATAG AGAATTTTCT CCTCTTCTGT
  361 GCCTTGGCAA TGAAGAACAT ATCTCATTAT ATCCAGATTT TCATAGACTA TTAGAATTTC
  421 TACAGCTCCC ATTTCCAAAG CCTTTAGTGT ATCTTCAACG CCAAAACAGT ACTTGCCCGT
  481 GTCCTGGCTG ATTTCATCAA AGTATCGTCC TATTAATTTC TTCTCTTGAA TGAATTTCAC
  541 GTTGGAGAGG ACTTCAGTAG ATAACTCAAT AGCTTGGTTG AATCCATTTT CACCACCATA
  601 GGATATATCA ACTAATTTTA AAACTTTTGA TTGTAACCTC TGATCAAACA TATCAGATTG
  661 ACTTAGTTCA GTTTTAAAGT CAGCGGATCC AGCTAAAACT AGACCAGCCA CATTCACTTT
  721 GTCCCCAGAA ATAAACAGCT GCACAGCAGT CTCTGCTACT TTCCGAACAT AGTTATGTCG
  781 CTTTTCCATT CTTAAACGGG CAAAACGCAA GGCTGACTGA CCTCCTCTAC CGTGTTTCTT
  841 TGGGAGATCC ACAGTGAATT TGTGCAGGAC TTCTCTTGTG TTTCCTTGGA GTGTGCCAAA
  901 AAGTGCACCA CTACCATCTA TTACAATGAA ATCCAAACTT GCTATCATCT GGAAGTAGTG
  961 CTGTAAGGAG CCTCTGTATG GGAATTTGTT TGTCACACAA ATACCATGAC GTATTAATTG
1021 GTTTGAAAAG
//
Primer: pGEX-ptac_up-F RDB13269_A7Drc.seq
>13269_13269_A7Dr_pGEX-ptac_up-F_C12_09_ABI24.ab1
    1 ATAAGCCGCT CATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
   61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
  121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
  181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
  241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
  301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
  361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
  421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
  481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
  541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
  601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
  661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
  721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
  781 GGAAGTTCTG TTCCAGGGGC CCCTGGGATC CCCGGAATTC ATGGCGGACG ACCCCAGTGC
  841 TGCCGACAGG AACGTGGAGA TCTGGAAGAT CAAGAAGCTC ATTTAAGAGC TTTGGAGGCG
  901 GCCCGCGGCA ATGGCACCAG CATGATATCA TTGATCATTC CTCCCAAAGA CCAGATTTTC
  961 ACGAGTGGCA AAAATGTTAG CGGGATGAGT TTGGGAACTG CATCTAACAT TAAGTCACGA
 1021 GTTAAACCCG CCTTTTCAGT CTGGGAGCAT TTACATTCTT GTTACAACCA AGACTTCAAC
 1081 CTTTATTAAC AAAGTACTCG AAACTGATCT TGCTTGGCTA TTACTTGTGT GGAAACAATT
1141 TTGTTTAACC AGGAG
//
Primer: M13_-40 RDB13269_A7Drd.seq
>13269_13269_A7Dr_M13(-40)_C10_07_ABI24.ab1
    1 CGGCCGCTCA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
   61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
  121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  241 TAGCGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
  841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
  901 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC
  961 CAGTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCCAG
 1021 ACTGGAGGTG GCACGCGATC AGCACGACTG TTTTGCCGGC AGCGGTGTGC ACGGCGGGAT
1081 TGGAATGTAA TCAGCTCCGG CCATCGCCGC TTCCAACCAT ATTTCCCCGC GCGAATA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl