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pGEX6P-eRF1 (#RDB13269)

Expression vector of human eRF1

Clone info. Expression vector of human eRF1.
Comment Expression was confirmed by depositor with Western blot and CBB staining.
Vector backbone pGEX6P (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human eRF1 cDNA
Depositor|Developer Imataka, Hiroaki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13269 pGEX6P-eRF1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-eRF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13269).

Reference section:

Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13269_A7Drp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB13269_A7Dra.seq check
>13269_13269_A7Dr_pGEX-F2_A10_01_ABI24.ab1
    1 GCTACTAGTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC
   61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
  121 CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCCC GGAATTCATG
  181 GCGGACGACC CCAGTGCTGC CGACAGGAAC GTGGAGATCT GGAAGATCAA GAAGCTCATT
  241 AAGAGCTTGG AGGCGGCCCG CGGCAATGGC ACCAGCATGA TATCATTGAT CATTCCTCCC
  301 AAAGACCAGA TTTCACGAGT GGCAAAAATG TTAGCGGATG AGTTTGGAAC TGCATCTAAC
  361 ATTAAGTCAC GAGTAAACCG CCTTTCAGTC CTGGGAGCCA TTACATCTGT ACAACAAAGA
  421 CTCAAACTTT ATAACAAAGT ACCTCCAAAT GGTCTGGTTG TATACTGTGG AACAATTGTA
  481 ACAGAAGAAG GAAAGGAAAA GAAAGTCAAC ATTGACTTTG AACCTTTCAA ACCAATTAAT
  541 ACGTCATTGT ATTTGTGTGA CAACAAATTC CATACAGAGG CTCTTACAGC ACTACTTTCA
  601 GATGATAGCA AGTTTGGATT CATTGTAATA GATGGTAGTG GTGCACTTTT TGGCACACTC
  661 CAAGGAAACA CAAGAGAAGT CCTGCACAAA TTCACTGTGG ATCTCCCAAA GAAACACGGT
  721 AGAGGAGGTC AGTCAGCCTT GCGTTTTGCC CGTTTAAGAA TGGAAAAGCG ACATAACTAT
  781 GTTCGGAAAG TAGCAGAGAC TGCTGTGCAG CTGTTTATTT CTGGGGACAA AGTGAATGTG
  841 GCTGGTCTAG TTTTAGCTGG ATCCGCTGAC TTTAAAACTG AACTAAGTCA ATCTGATATG
  901 TTTGATCAGA GGTTACAATC AAAAGTTTTA AAATTAGTTG ATATATCCTA TGGTGGTGAA
  961 AATGGATTCA ACCCAAGCTA TTTGAGTTAT CTACTGAAGT CCTCTCTACG TGAAATTCAT
 1021 TCAAGAGAGA AATTATAGGA CGATACTTTG AATGAAATCA GCCAGGGACA CGGGCAAGTT
1081 ACCTGGTTTT GCCGATTGGA AGGATACACT AAAGGCCTTT GGGAAAATTG GGGCAC
//
Primer: pGEX-R
Sequence file: RDB13269_A7Drb.seq check
>13269_13269_A7Dr_pGEX-R_B10_04_ABI24.ab1
    1 TCAATAAAGG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGCGGCC GCTCGAGCTA
   61 GTAGTCATCA AGGTCAAAAA ATTCATCGTC TCCTCCTTGG TATTCCATTC CCTGGAAATC
  121 TACTCGGTAC CGCAAGATAC CTCCAATTCC ACCAAATCCT TTCACAAACT GAGACCCTTC
  181 TTGTGATTTA TCTGTGACAA TTTCCAACGT AGCTCCAAAT TTTTTATAGT TGTTAGCAAA
  241 CCATTCCAAC AGGGGCATGC TCTCGATAAG CTCATGTTCC TGTCCGGTCT CTTTGTCTGT
  301 GAAATGAGAT TTATCCTTTT CTTGCTCTGG AGTTAGATAG AGAATTTTCT CCTCTTCTGT
  361 GCCTTGGCAA TGAAGAACAT ATCTCATTAT ATCCAGATTT TCATAGACTA TTAGAATTTC
  421 TACAGCTCCC ATTTCCAAAG CCTTTAGTGT ATCTTCAACG CCAAAACAGT ACTTGCCCGT
  481 GTCCTGGCTG ATTTCATCAA AGTATCGTCC TATTAATTTC TTCTCTTGAA TGAATTTCAC
  541 GTTGGAGAGG ACTTCAGTAG ATAACTCAAT AGCTTGGTTG AATCCATTTT CACCACCATA
  601 GGATATATCA ACTAATTTTA AAACTTTTGA TTGTAACCTC TGATCAAACA TATCAGATTG
  661 ACTTAGTTCA GTTTTAAAGT CAGCGGATCC AGCTAAAACT AGACCAGCCA CATTCACTTT
  721 GTCCCCAGAA ATAAACAGCT GCACAGCAGT CTCTGCTACT TTCCGAACAT AGTTATGTCG
  781 CTTTTCCATT CTTAAACGGG CAAAACGCAA GGCTGACTGA CCTCCTCTAC CGTGTTTCTT
  841 TGGGAGATCC ACAGTGAATT TGTGCAGGAC TTCTCTTGTG TTTCCTTGGA GTGTGCCAAA
  901 AAGTGCACCA CTACCATCTA TTACAATGAA ATCCAAACTT GCTATCATCT GGAAGTAGTG
  961 CTGTAAGGAG CCTCTGTATG GGAATTTGTT TGTCACACAA ATACCATGAC GTATTAATTG
1021 GTTTGAAAAG
//
Primer: pGEX-ptac_up-F
Sequence file: RDB13269_A7Drc.seq check
>13269_13269_A7Dr_pGEX-ptac_up-F_C12_09_ABI24.ab1
    1 ATAAGCCGCT CATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
   61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
  121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
  181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
  241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
  301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
  361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
  421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
  481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
  541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
  601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
  661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
  721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
  781 GGAAGTTCTG TTCCAGGGGC CCCTGGGATC CCCGGAATTC ATGGCGGACG ACCCCAGTGC
  841 TGCCGACAGG AACGTGGAGA TCTGGAAGAT CAAGAAGCTC ATTTAAGAGC TTTGGAGGCG
  901 GCCCGCGGCA ATGGCACCAG CATGATATCA TTGATCATTC CTCCCAAAGA CCAGATTTTC
  961 ACGAGTGGCA AAAATGTTAG CGGGATGAGT TTGGGAACTG CATCTAACAT TAAGTCACGA
 1021 GTTAAACCCG CCTTTTCAGT CTGGGAGCAT TTACATTCTT GTTACAACCA AGACTTCAAC
 1081 CTTTATTAAC AAAGTACTCG AAACTGATCT TGCTTGGCTA TTACTTGTGT GGAAACAATT
1141 TTGTTTAACC AGGAG
//
Primer: M13_-40
Sequence file: RDB13269_A7Drd.seq check
>13269_13269_A7Dr_M13(-40)_C10_07_ABI24.ab1
    1 CGGCCGCTCA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
   61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
  121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  241 TAGCGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
  841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
  901 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC
  961 CAGTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCCAG
 1021 ACTGGAGGTG GCACGCGATC AGCACGACTG TTTTGCCGGC AGCGGTGTGC ACGGCGGGAT
1081 TGGAATGTAA TCAGCTCCGG CCATCGCCGC TTCCAACCAT ATTTCCCCGC GCGAATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.