Expression vector of human eRF1
Clone info. | Expression vector of human eRF1. |
---|---|
Comment | Expression was confirmed by depositor with Western blot and CBB staining. |
Vector backbone | pGEX6P (Plasmid) |
Size of vector backbone | 5.0 kb |
Selectable markers | Amp^r |
Gene/insert name | Human eRF1 cDNA |
Depositor|Developer | Imataka, Hiroaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB13269 | pGEX6P-eRF1 | DNA solution |
Materials & Methods section:
The pGEX6P-eRF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13269). |
Reference section:
Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13269_A7Drp1.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: pGEX-F2 Sequence file: RDB13269_A7Dra.seq |
---|
>13269_13269_A7Dr_pGEX-F2_A10_01_ABI24.ab1 1 GCTACTAGTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC 61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT 121 CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCCC GGAATTCATG 181 GCGGACGACC CCAGTGCTGC CGACAGGAAC GTGGAGATCT GGAAGATCAA GAAGCTCATT 241 AAGAGCTTGG AGGCGGCCCG CGGCAATGGC ACCAGCATGA TATCATTGAT CATTCCTCCC 301 AAAGACCAGA TTTCACGAGT GGCAAAAATG TTAGCGGATG AGTTTGGAAC TGCATCTAAC 361 ATTAAGTCAC GAGTAAACCG CCTTTCAGTC CTGGGAGCCA TTACATCTGT ACAACAAAGA 421 CTCAAACTTT ATAACAAAGT ACCTCCAAAT GGTCTGGTTG TATACTGTGG AACAATTGTA 481 ACAGAAGAAG GAAAGGAAAA GAAAGTCAAC ATTGACTTTG AACCTTTCAA ACCAATTAAT 541 ACGTCATTGT ATTTGTGTGA CAACAAATTC CATACAGAGG CTCTTACAGC ACTACTTTCA 601 GATGATAGCA AGTTTGGATT CATTGTAATA GATGGTAGTG GTGCACTTTT TGGCACACTC 661 CAAGGAAACA CAAGAGAAGT CCTGCACAAA TTCACTGTGG ATCTCCCAAA GAAACACGGT 721 AGAGGAGGTC AGTCAGCCTT GCGTTTTGCC CGTTTAAGAA TGGAAAAGCG ACATAACTAT 781 GTTCGGAAAG TAGCAGAGAC TGCTGTGCAG CTGTTTATTT CTGGGGACAA AGTGAATGTG 841 GCTGGTCTAG TTTTAGCTGG ATCCGCTGAC TTTAAAACTG AACTAAGTCA ATCTGATATG 901 TTTGATCAGA GGTTACAATC AAAAGTTTTA AAATTAGTTG ATATATCCTA TGGTGGTGAA 961 AATGGATTCA ACCCAAGCTA TTTGAGTTAT CTACTGAAGT CCTCTCTACG TGAAATTCAT 1021 TCAAGAGAGA AATTATAGGA CGATACTTTG AATGAAATCA GCCAGGGACA CGGGCAAGTT 1081 ACCTGGTTTT GCCGATTGGA AGGATACACT AAAGGCCTTT GGGAAAATTG GGGCAC // |
Primer: pGEX-R Sequence file: RDB13269_A7Drb.seq |
>13269_13269_A7Dr_pGEX-R_B10_04_ABI24.ab1 1 TCAATAAAGG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGCGGCC GCTCGAGCTA 61 GTAGTCATCA AGGTCAAAAA ATTCATCGTC TCCTCCTTGG TATTCCATTC CCTGGAAATC 121 TACTCGGTAC CGCAAGATAC CTCCAATTCC ACCAAATCCT TTCACAAACT GAGACCCTTC 181 TTGTGATTTA TCTGTGACAA TTTCCAACGT AGCTCCAAAT TTTTTATAGT TGTTAGCAAA 241 CCATTCCAAC AGGGGCATGC TCTCGATAAG CTCATGTTCC TGTCCGGTCT CTTTGTCTGT 301 GAAATGAGAT TTATCCTTTT CTTGCTCTGG AGTTAGATAG AGAATTTTCT CCTCTTCTGT 361 GCCTTGGCAA TGAAGAACAT ATCTCATTAT ATCCAGATTT TCATAGACTA TTAGAATTTC 421 TACAGCTCCC ATTTCCAAAG CCTTTAGTGT ATCTTCAACG CCAAAACAGT ACTTGCCCGT 481 GTCCTGGCTG ATTTCATCAA AGTATCGTCC TATTAATTTC TTCTCTTGAA TGAATTTCAC 541 GTTGGAGAGG ACTTCAGTAG ATAACTCAAT AGCTTGGTTG AATCCATTTT CACCACCATA 601 GGATATATCA ACTAATTTTA AAACTTTTGA TTGTAACCTC TGATCAAACA TATCAGATTG 661 ACTTAGTTCA GTTTTAAAGT CAGCGGATCC AGCTAAAACT AGACCAGCCA CATTCACTTT 721 GTCCCCAGAA ATAAACAGCT GCACAGCAGT CTCTGCTACT TTCCGAACAT AGTTATGTCG 781 CTTTTCCATT CTTAAACGGG CAAAACGCAA GGCTGACTGA CCTCCTCTAC CGTGTTTCTT 841 TGGGAGATCC ACAGTGAATT TGTGCAGGAC TTCTCTTGTG TTTCCTTGGA GTGTGCCAAA 901 AAGTGCACCA CTACCATCTA TTACAATGAA ATCCAAACTT GCTATCATCT GGAAGTAGTG 961 CTGTAAGGAG CCTCTGTATG GGAATTTGTT TGTCACACAA ATACCATGAC GTATTAATTG 1021 GTTTGAAAAG // |
Primer: pGEX-ptac_up-F Sequence file: RDB13269_A7Drc.seq |
>13269_13269_A7Dr_pGEX-ptac_up-F_C12_09_ABI24.ab1 1 ATAAGCCGCT CATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG 61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT 121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT 181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG 241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG 301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT 361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA 421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA 481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC 541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT 601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA 661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG 721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT 781 GGAAGTTCTG TTCCAGGGGC CCCTGGGATC CCCGGAATTC ATGGCGGACG ACCCCAGTGC 841 TGCCGACAGG AACGTGGAGA TCTGGAAGAT CAAGAAGCTC ATTTAAGAGC TTTGGAGGCG 901 GCCCGCGGCA ATGGCACCAG CATGATATCA TTGATCATTC CTCCCAAAGA CCAGATTTTC 961 ACGAGTGGCA AAAATGTTAG CGGGATGAGT TTGGGAACTG CATCTAACAT TAAGTCACGA 1021 GTTAAACCCG CCTTTTCAGT CTGGGAGCAT TTACATTCTT GTTACAACCA AGACTTCAAC 1081 CTTTATTAAC AAAGTACTCG AAACTGATCT TGCTTGGCTA TTACTTGTGT GGAAACAATT 1141 TTGTTTAACC AGGAG // |
Primer: M13_-40 Sequence file: RDB13269_A7Drd.seq |
>13269_13269_A7Dr_M13(-40)_C10_07_ABI24.ab1 1 CGGCCGCTCA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA 61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG 121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG 181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC 241 TAGCGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG 301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG 361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC 421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG 481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG 541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG 601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA 661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC 721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA 781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG 841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC 901 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC 961 CAGTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCCAG 1021 ACTGGAGGTG GCACGCGATC AGCACGACTG TTTTGCCGGC AGCGGTGTGC ACGGCGGGAT 1081 TGGAATGTAA TCAGCTCCGG CCATCGCCGC TTCCAACCAT ATTTCCCCGC GCGAATA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP] |
---|---|
reference | Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838. |
Featured content