RDB13232 - pEGFP-C1-SKIP/PLEKHM2

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pEGFP-C1-SKIP/PLEKHM2

Expression vector of SKIP/PLEKHM2

Catalog number	RDB13232
Resource name	pEGFP-C1-SKIP/PLEKHM2
Clone info.	Expression vector of SKIP/PLEKHM2.
Vector backbone	pEGFP-C1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli); Neo^r (mammalian cells)
Gene/insert name	Human SKIP/PLEKHM2 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Other clones in our bank

human SKIP/PLEKHM2

External Database
Human SKIP/PLEKHM2

Reference sequence

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep., 5: 8238 (2015).)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep., 5: 8238 (2015).)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci Rep., 5: 8238 (2015).)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13232	pEGFP-C1-SKIP/PLEKHM2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-SKIP/PLEKHM2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13232).

Reference section:

Ishida, M., Ohbayashi, N., Fukuda, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13232_A5Cvp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: insert 5' Sequence file: RDB13232_A5Cva.seq
>13232_13232_B1KoF_EGFP-C2_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAC AANTCCGGAC TCAGATCTAT GGAGCCGGGG 61 GAGGTGAAGG ACCGGATCCT GGAGAACATC TCGCTGTCGG TGAAGAAGTT GCAGAGCTAT 121 TTTGCTGCAT GTGAGGATGA GATCCCTGCC ATCCGGAACC ATGACAAGGT CCTACAGCGT 181 CTGTGTGAGC ACCTGGACCA CGCCCTGCTG TACGGACTGC AAGACCTCTC CTCTGGCTAC 241 TGGGTGCTCG TGGTGCATTT TACTCGGAGA GAGGCCATCA AGCAGATCGA GGTGCTGCAG 301 CACGTGGCCA CCAACCTGGG GCGCAGCCGT GCCTGGCTGT ACCTGGCCCT CAACGAGAAC 361 TCCTTGGAGA GCTACCTGCG GTTGTTCCAG GAGAACCTGG GCCTGCTGCA TAAGTACTAC 421 GTCAAGAATG CCCTGGTCTG CAGCCACGAT CACCTGACGC TCTTCCTGAC CTTGGTGTNN 481 GGGCTAGAGT TCATTCGTTT CGAGCTGGAT CTGGATGCCC CTTACCTAGA CCTGGCCCCC 541 TACATGCCCG ACTACTACAA ACCTCAGTAC CTGCTGGACT TTGAAGACCG CCTTCCCAGC 601 TCGGTCCNCN GCTCANACNG TCTGTCCCTC NACTCTTTCA NTNCNTCANN TNCNCCNANC 661 NNNNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB13232_A5Cvb.seq
>13232_13232_B1KoF_SV40polyA_R2_H02_23_ABI24.ab1 1 NNNNNNNNNN NNTNNNNNNT NNGGGGGAGN NNNGGGAGGT TTTTTAAAGC AAGTAAAACC 61 TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC 121 GCGGTACCGT CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCAGCA CCAGGGGTCT 181 CGGGAGGCTC GGCCGCGGCA GAGACTGTCG CTCCGCTGCC AGGCGCTGTG GATGAGGCTC 241 AAGGCATCCT CGAATTTCTT CTTGTTGGAG GCTTCCTGGA TCGCCGTGTG GGGGAGGTCC 301 ACCTGATAGA TGGTTTTCCA CCCAGAGTTC AGTGCAGACA GCAATCGGTC CAGTTCAGAA 361 GTGCAGCTCA GGTAGATGAC CCAGGGCGGG AGGAGCTGCT GGCTGTCCTG GGAGAACTCC 421 AAGACGCAGT ACTCCTTGCC CGGCTCGGTG GAGACGGCGC TGATGTCGCC CAGCTTGGCT 481 GTGCCCAAAG AGCGGAAGAA GCTGGTCTGG CAATCCTCAT GGCACGTAAA GAGGCGGTCA 541 TCCGTGANGA CCAGGCAGCA NGGTATGCAN GGGCTGGGAG CTACGCCCTG GGGGANGANN 601 NCTTTGGANA CAGCCTGNCA GANANGCTGC ATCCACTCNG CCNTCTCGGC CTCNCTCNCN 661 GNNCTTANCT CCNGGCNNGG NCNNGNCNGA NANNANGANN NNNNANNNNN NNGGNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB13232_A5Cvc.seq
>13232_13232_A5Cv_1_CMV-Fowaed_D11_11.ab1 1 NNNNNNNNNN NNNNTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGNGGT CTATATAAGC 61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA 121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA 181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA 241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA 301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT 361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG 421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA 481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT 541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG 601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC 661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT 781 TCGTGACCGC CGCCGGGNTC ACTCTCGGCA TGNNCGANCT GTACNAGTCC GGACTCAGAT 841 CTATGGAGCC GNGGGANGTG AANGANCGGA TNCTGGANAA CATCTCGCTG TCGGNGAANA 901 AGTTGCANAN CNNTTTTGCT GNNTGTNANG NTGANATNCN NGNNNTCCNN NACCNTGNNN 961 NNNNCCNACA NNGNCTGNNN NANCNNNNNN NNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB13232_A5Cvd.seq
>13232_13232_A5Cv_1_SV40pro_F_v2_G11_20.ab1 1 NNNNNNNNNN NNNNGNNNNC NNNGCTTTTG NAAGNNCGAT CAAGAGACAG GNTGAGGNNC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TNCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GANTTCTTCT GAGCGGGANT CTGGGGTNNG AAATGANCGA NNNAGCGACG 901 CCCAACCNGN NATCNCGANA TTNCGANNCC NNCGCCGCCN TCTATGAAAG GNNNGGNTNC 961 NNANNCNNTT TNCNGNNNGN NNNNNNGNNN NNNCNNNNNN CNCNGGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl