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pEGFP-C1-SKIP/PLEKHM2 (#RDB13232)

Expression vector of SKIP/PLEKHM2

Clone info. Expression vector of SKIP/PLEKHM2.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli); Neo^r (mammalian cells)
Gene/insert name Human SKIP/PLEKHM2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep., 5: 8238 (2015).)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13232 pEGFP-C1-SKIP/PLEKHM2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-SKIP/PLEKHM2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13232).

Reference section:

Ishida, M., Ohbayashi, N., Fukuda, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13232_A5Cvp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: insert 5'
Sequence file: RDB13232_A5Cva.seq check
>13232_13232_B1KoF_EGFP-C2_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAC AANTCCGGAC TCAGATCTAT GGAGCCGGGG
   61 GAGGTGAAGG ACCGGATCCT GGAGAACATC TCGCTGTCGG TGAAGAAGTT GCAGAGCTAT
  121 TTTGCTGCAT GTGAGGATGA GATCCCTGCC ATCCGGAACC ATGACAAGGT CCTACAGCGT
  181 CTGTGTGAGC ACCTGGACCA CGCCCTGCTG TACGGACTGC AAGACCTCTC CTCTGGCTAC
  241 TGGGTGCTCG TGGTGCATTT TACTCGGAGA GAGGCCATCA AGCAGATCGA GGTGCTGCAG
  301 CACGTGGCCA CCAACCTGGG GCGCAGCCGT GCCTGGCTGT ACCTGGCCCT CAACGAGAAC
  361 TCCTTGGAGA GCTACCTGCG GTTGTTCCAG GAGAACCTGG GCCTGCTGCA TAAGTACTAC
  421 GTCAAGAATG CCCTGGTCTG CAGCCACGAT CACCTGACGC TCTTCCTGAC CTTGGTGTNN
  481 GGGCTAGAGT TCATTCGTTT CGAGCTGGAT CTGGATGCCC CTTACCTAGA CCTGGCCCCC
  541 TACATGCCCG ACTACTACAA ACCTCAGTAC CTGCTGGACT TTGAAGACCG CCTTCCCAGC
  601 TCGGTCCNCN GCTCANACNG TCTGTCCCTC NACTCTTTCA NTNCNTCANN TNCNCCNANC
661 NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB13232_A5Cvb.seq check
>13232_13232_B1KoF_SV40polyA_R2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNTNNNNNNT NNGGGGGAGN NNNGGGAGGT TTTTTAAAGC AAGTAAAACC
   61 TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC
  121 GCGGTACCGT CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCAGCA CCAGGGGTCT
  181 CGGGAGGCTC GGCCGCGGCA GAGACTGTCG CTCCGCTGCC AGGCGCTGTG GATGAGGCTC
  241 AAGGCATCCT CGAATTTCTT CTTGTTGGAG GCTTCCTGGA TCGCCGTGTG GGGGAGGTCC
  301 ACCTGATAGA TGGTTTTCCA CCCAGAGTTC AGTGCAGACA GCAATCGGTC CAGTTCAGAA
  361 GTGCAGCTCA GGTAGATGAC CCAGGGCGGG AGGAGCTGCT GGCTGTCCTG GGAGAACTCC
  421 AAGACGCAGT ACTCCTTGCC CGGCTCGGTG GAGACGGCGC TGATGTCGCC CAGCTTGGCT
  481 GTGCCCAAAG AGCGGAAGAA GCTGGTCTGG CAATCCTCAT GGCACGTAAA GAGGCGGTCA
  541 TCCGTGANGA CCAGGCAGCA NGGTATGCAN GGGCTGGGAG CTACGCCCTG GGGGANGANN
  601 NCTTTGGANA CAGCCTGNCA GANANGCTGC ATCCACTCNG CCNTCTCGGC CTCNCTCNCN
661 GNNCTTANCT CCNGGCNNGG NCNNGNCNGA NANNANGANN NNNNANNNNN NNGGNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB13232_A5Cvc.seq check
>13232_13232_A5Cv_1_CMV-Fowaed_D11_11.ab1
    1 NNNNNNNNNN NNNNTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGNGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGNTC ACTCTCGGCA TGNNCGANCT GTACNAGTCC GGACTCAGAT
  841 CTATGGAGCC GNGGGANGTG AANGANCGGA TNCTGGANAA CATCTCGCTG TCGGNGAANA
  901 AGTTGCANAN CNNTTTTGCT GNNTGTNANG NTGANATNCN NGNNNTCCNN NACCNTGNNN
961 NNNNCCNACA NNGNCTGNNN NANCNNNNNN NNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB13232_A5Cvd.seq check
>13232_13232_A5Cv_1_SV40pro_F_v2_G11_20.ab1
    1 NNNNNNNNNN NNNNGNNNNC NNNGCTTTTG NAAGNNCGAT CAAGAGACAG GNTGAGGNNC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TNCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GANTTCTTCT GAGCGGGANT CTGGGGTNNG AAATGANCGA NNNAGCGACG
  901 CCCAACCNGN NATCNCGANA TTNCGANNCC NNCGCCGCCN TCTATGAAAG GNNNGGNTNC
961 NNANNCNNTT TNCNGNNNGN NNNNNNGNNN NNNCNNNNNN CNCNGGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]