Resource data sheet
DNA Bank Top

CSIV-CMV-mOKS-IRES2-Venus (#RDB12954)

Lentivirus vector plasmid of mouse Oct3/4, Klf4 and Sox2 with Venus fluorescent protein marker driven by CMV promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB12954.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Lentivirus vector plasmid of mouse Oct3/4, Klf4 and Sox2 with Venus fluorescent protein marker driven by CMV promoter.
Comment Nucleotide sequences of Vector-insert junction and variable region were checked. Known difference (at least) : mOct3/4, C to T substitution at 159nt (Pro to Ser) compared with NM_013633.3; mKlf4, G to A substitution at 1003nt (Pro to Pro, synonym), and T to C substitution at 1999nt (Cys to Cys, synonym) compared with NM_010637.3. pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) are popular choices for lentivirus packaging.
Vector backbone CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Mouse Oct3/Oct4 cDNA Mouse Klf4 cDNA Mouse Sox2 cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee
RDB12954 CSIV-CMV-mOKS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSIV-CMV-mOKS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12954).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles