RIKEN DNA Bank Mouse Resource

RIKEN DNA Bank Mouse Resource - Pou5f1

Gene ID	NCBI Gene 18999 \| KEGG mmu:18999
Gene Symbol	Pou5f1
Protein Name	POU domain, class 5, transcription factor 1
Synonyms	NF-A3\|Oct-3\|Oct-3/4\|Oct-4\|Oct3\|Oct3/4\|Oct4\|Otf-3\|Otf-4\|Otf3\|Otf3-rs7\|Otf3g\|Otf4
Featured content	Signaling pathways regulating pluripotency of stem cells (mouse)

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Ortholog resource in our bank

Pou5f1

External dsatabase

mouse Pou5f1

Individualy Deposited Resource

Catalog number	Name of Resource	Description	CDS comparison
Catalog number	Name of Resource	Description	Refered (NCBI mRNA)	CDS status⁽¹⁾
RDB13378	pRZ-mOct4-CNL	Lentiviral reporter plasmid of mouse Oct4 by the Cyan Nano-lantern.
RDB13138	PB-TAC-WTSI	piggyBac transposon system for dox-inducible iPS cell generation (WTSI cassette with mCherry reporter)
RDB13137	PB-TAC-EB-C5	piggyBac transposon system for dox-inducible iPS cell generation (EB-C5 cassette with mCherry reporter)
RDB13136	PB-TAC-STEMCCA	piggyBac transposon system for dox-inducible iPS cell generation (STEMCCA cassette with mCherry reporter)
RDB13135	PB-TAC-OK+9MS	piggyBac transposon system for dox-inducible iPS cell generation (OK+9MS cassette with mCherry reporter)
RDB13134	PB-TAC-MKOS	piggyBac transposon system for dox-inducible iPS cell generation (MKOS cassette with mCherry reporter)
RDB13133	PB-TAC-OKMS	piggyBac transposon system for dox-inducible iPS cell generation (OKMS cassette with mCherry reporter)
RDB13132	PB-TAC-OSKM	piggyBac transposon system for dox-inducible iPS cell generation (OSKM cassette with mCherry reporter)
RDB12961	CSIV-TRE-mOct3/4-CMV-KT	Lentivirus vector plasmid of mouse Oct3/4 driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
RDB12960	CS-UbC-mOct3/4-IRES2-Venus	Lentivirus vector plasmid of mouse Oct3/4 with Venus fluorescent protein marker driven by UbC promoter.
RDB12955	CSII-EF-mOct3/4-IRES2-Venus	Lentivirus vector plasmid of mouse Oct3/4 with Venus fluorescent protein marker driven by EF-1 alpha promoter.
RDB12954	CSIV-CMV-mOKS-IRES2-Venus	Lentivirus vector plasmid of mouse Oct3/4, Klf4 and Sox2 with Venus fluorescent protein marker driven by CMV promoter.
RDB12115	pPthc-Oct3/4	Exchange vector to introduce Oct3 into the ROSA locus
RDB11852	pPB-hCMV*1-Oct3-pA	piggyBac expression vector of mOct3/4. TRE and a min-CMV promoter.
RDB11831	pPBCAG-Oct3P2APMKO1-IP	piggyBac expression vector of Oct3/4::P2A::Lyn PM Tgt Seq::mKO. CAG promoter and puromycin resistance gene.
RDB11830	pPBCAG-Oct3P2APMKeima-IP	piggyBac expression vector of Oct3/4::P2A::Lyn PM Tgt Seq::mKeima Red. CAG promoter, puromycin resistance gene.
RDB11639	pPBCAG-Oct3P2ASox2-pA	piggyBac expression vector contains mOct3/4::P2A::mSox2 driven by a CAG promoter.
RDB11615	pPB-Ins2-CMVp-mCherry-IP	piggyBac expression vector contains mCherry and puromycin resistance gene via IRES driven by a minimal CMV promoter, inserted between the Evx2::Hoxd13 Intergenic regions.
RDB11584	pPBCAG-Oct3P2ASox2-IP	piggyBac expression vector of mOct3/4::P2A::mSox2. CAG promoter and puromycin resistance gene.
RDB11514	pPB-Oct3-9827luc-PGKpacpA(A)	piggyBac vector contains a luciferase gene under the control of the Oct3 upstream region. PGK promoter and puromycin resistance gene.
RDB10986	pOct4-luc (-1938)	Reporter construct of mouse Oct3/4 (1.9 kb).
RDB10754	pOct4-luc (-1281)	Reporter construct of mouse Oct3/4 (1.3 kb).
RDB10753	pOct4-luc (-2177)	Reporter construct of mouse Oct3/4 (2.2 kb).
RDB10752	pOct4-luc (-285)	Reporter construct of mouse Oct3/4 (0.3 kb).
RDB10551	pPyCAG-EGFPOct3GR-IP	Expression clone of EGFP-mouse Oct3wt-GR fusion
RDB10471	pCAG-CY-Oct3	Expression clone of mouse Oct3
RDB09895	pOct4-luc	Reporter construct of mouse Oct3/4 (0.5 kb).
RDB09609	pPyCAG-Oct3wt-IRESpuropA	Expression clone of mouse Oct-3/4
RDB09330	Oct4IRESzeocinpA KO	KO vector of mouse Oct4
RDB09193	pBlKS(-) Oct-3cDNA 21A	Plasmid clone of mouse Oct3/4
RDB08513	CSII-CMV-mOct3/4-IRES-Venus	Lentivirus vector plasmid of mouse Oct3/4 with Venus fluorescent protein marker driven by CMV promoter.

(1) CDS status was determined by comparing the plasmid sequence with NCBI RefSeq mRNA.

webcatalog20240727.tab

NIA/NIH Mouse cDNA Clone

Plasmid request [in Japanese] [in English]

Catalog number	Clone name	Vector	Sequence		Status
Catalog number	Clone name	Vector	5' terminal EST, submitted in DDBJ⁽¹⁾	3' terminal EST, submitted in DDBJ⁽¹⁾	Status
MCH011369	H3028H01	pSPORT1	BG078528	BG065240

♦ Full length sequence is not available. The clone could differ from the NCBI mRNA reference sequence.
(1) Actual EST sequence either 5' or 3' terminal end. Data were submitted to the DNA Data Bank of Japan (DDBJ).
♦ NIA/NIH Mouse cDNA clone was deposited by Dr Minoru Ko (Tanaka et al., PNAS 97, 9127-9132, 2000. VanBuren V, et al. Genome Research 12, 1999-2003, 2002) at the National Institute on Aging, the National Institutes of Health, U.S.A.

NIAmouse22k_2024May11.csv

Mouse BAC clone

Clone request [in Japanese] [in English]

BAC clone search	[map around Gene ID 18999] (link to our BAC browser)

♦ Every single our BAC clones have analyzed their terminal end sequences to map on the genome. Most of them are assigned by both ends and indicated with blue line on the genome browser (they likely contain the region). Others are assigned only by a single end and indicated with red line on the genome browser (containing piece is unsure).
♦ Reasons that we did not use the other end result for mapping are: (1) Data was not suitable for BLAST analysis (short read, repeats, no data or such stuff); (2) Result of BLAST analysis is unlikely [two ends are, too far away (>0.3 Mb), distinct chromosome, same orientation, or such incomprehensible].
♦ Full length sequence is not available.

2025.11.13

Mus_musculus_gene_info230514.csv - RDB_mmu_GIxxxxxxxxx_html_240727.pl