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pFBD-Hs alpha1(K40R)CGH/beta3CGF

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin

Catalog number RDB12754
Resource name pFBD-Hs alpha1(K40R)CGH/beta3CGF
Alternative name FLAG-and His-tagged Human alpha1 beta3 tubulin(K40R)
Clone info. Plasmid clone for baculoviral expression of alpha1(NP_006073) and beta3 (NP_006077) tubulin. K40R mutant form.
Comment Human alpha1-tubulin, in which lysine 40 is substituted with arginine, is fused to linker (GGSGG) and His-tag (HHHHHHHH) at its C-terminus. Human b3-tubulin is fused to linker (GGSGG) and FLAG-tag (DYKDDDDK) at its C-terminus.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Human alpha1-Tb cDNA Human eta3-Tb cDNA
Depositor|Developer Muto, Etsuko |
Other clones in our bank

External Database
Human alpha1-Tb Human eta3-Tb

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (FEBS Lett., 587, 3450-3455 (2013))を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB12754 pFBD-Hs alpha1(K40R)CGH/beta3CGF DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Purification of Recombinant Tubulin (pdf)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB12754_A4Dfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pPolyhedrin_F
        Region: polyhedrin pro, L21, insert2-5'
        Sequence file: RDB12754_A4Dfa.seq check
        >12754_3_pPolyhedrin_F_E05_14.ab1
            1 CAATTTTTTA CTTGTTTTCG TAACAGTTTT GTAATAAAAA AACCTATAAA TATTCCGGAT
           61 TATTCATACC GTCCCACCAT CGGGCGCGGA TCCCGGTCCG AAGCGCGCGG AATTCAAAGG
          121 CCTACGTCGA CGAGCTCACT AGTAACTCCT AAAAAACCGC CACCATGCGC GAGTGCATCT
          181 CCATCCACGT GGGCCAGGCT GGTGTCCAGA TCGGCAACGC TTGCTGGGAG CTGTACTGCC
          241 TGGAGCACGG TATCCAGCCC GACGGCCAGA TGCCCTCCGA CCGCACCATC GGTGGCGGCG
          301 ACGACTCCTT CAACACCTTC TTCTCCGAAA CCGGTGCTGG CAAGCACGTG CCCCGCGCTG
          361 TGTTCGTGGA TCTGGAACCC ACCGTGATCG ACGAAGTGCG TACCGGCACC TACCGTCAGC
          421 TGTTCCACCC CGAGCAACTG ATCACCGGCA AGGAGGACGC TGCTAACAAC TACGCTCGTG
          481 GTCACTACAC CATCGGCAAG GAAATCATCG ACCTGGTCCT GGACCGTATC CGCAAGCTGG
          541 CTGACCAGTG CACCGGCCTG CAGGGATTCC TCGTGTTCCA CTCCTTCGGT GGTGGAACCG
          601 GTTCTGGTTT CACCTCCCTG CTGATGGAAC GTCTGTCTGT GGACTACGGC AAGAAGTCCA
          661 AGTTGGAGTT CTCCATCTAC CCCGCTCCCC AGGTGTCCAC CGCTGTGGTC GAGCCCTACA
          721 ACTCCATCCT GACCACCCAC ACCACCCTGG AACACTCCGA CTGCGCTTTC ATGGTGGACA
          781 ACGAGGCTAT CTACGACATC TGCCGTCGTA ACCTGGACAT CGAGCGTCCC ACCTACACCA
          841 ACCTGAACCG TCTGATCTCC CAGATCGTGT CCTCCATCAC CGCTTCCCTG CGTTTCGACG
          901 GTGCTCTGAA CGTGGACCTG ACCGAGTTCC AGACCAACCT GGTGCCCTAC CCCCGTATCC
          961 ACTTCCCCCT GGCTACCTAC GCTCCCGTGA TCTCCGCTGA GAAGCTTACC ACGAGCAGCT
         1021 GTCCGTGGGC TGAGATCACC AAACGCCTGC TTCGAGCCCG CTAACAGATG GTCAAGTGCG
         1081 ACCCCCCGTC ACGGCCAGTA CATGGCTTGC TGCCTTGCTG TACGTGCGAA CGTGGTGGCT
        1141 AGGACGTGAC GGCTGCCTAT TCGGCTACCA TCTCAAGAAA CTA
        //
        Primer: ME-1290Rv
        Region: 8xHis,GGSGG, insert2-3'
        Sequence file: RDB12754_A4Dfb.seq check
        >12754_3_1290Rv_F05_17.ab1
            1 CTGTCCATTT CATTTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
           61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCC TCTAGTACTT CTCGACAAGC
          121 TTTTAGTGGT GATGGTGGTG ATGATGGTGA CCACCGGAAC CACCGTATTC CTCGCCTTCC
          181 TCTTCGCCCT CGCCCTCGAC GGAGTCGACG CCCACTTCCT CGTAGTCCTT TTCCAGAGCA
          241 GCCATGTCCT CGCGAGCCTC GGAGAACTCG CCCTCTTCCA TGCCTTCGCC CACGTACCAG
          301 TGCACGAAAG CGCGCTTAGC GTACATCAGG TCGAACTTGT GGTCCAGACG AGCCCAAGCC
          361 TCAGCGATAG CGGTGGTGTT GGACAGCATG CACACAGCAC GCTGCACCTT AGCCAAGTCG
          421 CCACCGGGCA CCACAGTGGG GGGCTGGTAG TTGATTCCGA CCTTGAAACC GGTGGGGCAC
          481 CAGTCCACGA ACTGGATGGA ACGCTTGGTC TTGATGGTAG CGATAGCAGC GTTCACGTCC
          541 TTAGGCACCA CGTCGCCACG GTACAGCAGG CAGCAAGCCA TGTACTTGCC GTGACGGGGG
          601 TCGCACTTGA CCATCTGGTT AGCGGGCTCG AAGCAGGCGT TGGTGATCTC AGCCACGGAC
          661 AGCTGCTCGT GGTAAGCCTT CTCAGCGGAG ATCACGGGAG CGTAGGTAGC CAGGGGGAAG
          721 TGGATACGGG GGTAGGGCAC CAGGTTGGTC TGGAACTCGG TCAGGTCCAC GTTCAGAGCA
          781 CCGTCGAAAC GCAGGGAAGC GGTGATGGAG GACACGATCT GGGAGATCAG ACGGTTCAGG
          841 TTGGTGTAGG TGGGACGCTC GATGTCCAGG TTACGACGGC AGATGTCGTA GATAGCCTCG
          901 TTGTCCACCA TGAAAGCGCA GTCGGAGTGT TCCAGGGTGG TGTGGGTGGT CAGGATGGAG
          961 TTGTAGGGGC TCGACCACAG CGGTGGACAC CTGGGGAGCG GGGTAGATGG AGAACTCCTA
         1021 CTTTGGACTT CTGCCGTAGT CCACAGACAG ACGTTCCAAT CAGCAGGGAG GTGAAACCAG
         1081 AACTGATCAC AACGAAGAGT GTACACGAGG ATTCCTGCAG GCTGTTGCAC TGTCCAGGCC
        1141 GAGCTTGGCC GGGAATATAC G
        //
        Primer: pP10_F
        Region: p10pro,21L,insert1-5'
        Sequence file: RDB12754_A4Dfc.seq check
        >12754_3_pP10_F_G05_20.ab1
            1 TTTAAATAAG GAATTTATTT ATCAAATCAT TTGTATATTA ATTAAAATAC TATACTGTAA
           61 ATTACATTTT ATTTACAATC ACTCGACGAA GACTTGATCA CCCGGGATCT CGAGAACTCC
          121 TAAAAAACCG CCACCATGCG CGAGATCGTG CACATCCAGG CTGGTCAGTG CGGTAACCAG
          181 ATCGGTGCTA AGTTCTGGGA GGTCATCTCC GACGAGCACG GTATCGACCC TTCCGGTAAC
          241 TACGTGGGTG ACTCCGACCT GCAGCTGGAG CGCATTTCCG TGTACTACAA CGAAGCCTCC
          301 TCCCACAAAT ACGTGCCTCG CGCTATCCTG GTGGACCTGG AGCCTGGTAC TATGGACTCT
          361 GTGCGCTCCG GTGCTTTCGG TCACCTGTTC CGCCCTGACA ACTTCATCTT CGGTCAGTCC
          421 GGTGCTGGTA ACAACTGGGC TAAGGGTCAC TACACCGAGG GTGCTGAGCT CGTCGACTCC
          481 GTGCTGGACG TGGTGCGCAA GGAGTGCGAG AACTGCGACT GCCTGCAGGG TTTCCAGCTG
          541 ACTCACTCCC TGGGTGGTGG TACCGGATCT GGTATGGGTA CCCTGCTGAT CTCCAAGGTG
          601 CGCGAGGAGT ACCCTGACCG CATCATGAAT ACCTTCTCCG TCGTGCCTTC CCCTAAGGTG
          661 TCCGACACCG TGGTGGAGCC TTACAACGCT ACCCTGTCCA TCCACCAACT GGTGGAGAAC
          721 ACCGACGAGA CCTACTGCAT CGACAACGAG GCTCTGTACG ACATCTGCTT CCGCACCCTG
          781 AAGCTGGCTA CCCCTACCTA CGGTGACCTG AACCACCTGG TCTCCGCTAC CATGTCCGGT
          841 GTGACCACCT CCCTGCGCTT CCCTGGTCAG CTGAACGCTG ACCTGCGCAA AGCTGGCTGT
          901 GAACATGGTG CCTTTTCCCT CGCCTGCACT TCTTTCATGC CTGGTTTCGC TCCTCTGACC
          961 GCTCGCGGTT CCCCAGCAAT TACCGCGCTC TGACCGTGCC CTGAGCTGAC CCAGCCAGAA
         1021 TGTTCGACGC TAAGAACATG ATGGCTGCTT GCGACCCTCG GCCACCGGTC GGCTACCTGA
         1081 ACCGGTGGCC TACCGTGGTT CCGCGGGTCG GCATGTCATG AAGGAAGGTT GGACGAAGCA
         1141 GATGGCCTGG CTATCAGTCC AGGAAACCTC CCTCCTACCT TTCCGCTGGG GAAGATTGTG
        1201 AAAATCCCCT TAACACAC
        //
        Primer: HSV_tk_pA_R
        Region: FLAG,GGSGG,insert1-3'
        Sequence file: RDB12754_A4Dfd.seq check
        >12754_3_HSV_tk_pA_R_H05_23.ab1
            1 AGGGGGTCCT TTCCGGTATT GTCTTCTTCC GTGTTTCAGG TTAAGCCTCC CCCATCTCCC
           61 GGTACCGCAT GCTATGCATC AGCTGCTAGC TTACTTGTCA TCGTCATCTT TGTAGTCACC
          121 ACCGGAACCA CCCTTAGGAC CCTGAGCCTC GGACTCTTCC TCATCGTCCT CGTACATCTC
          181 ACCCTCTTCT TCAGCGGTAG CGTCCTGGTA CTGCTGGTAC TCGGACACCA GGTCGTTCAT
          241 GTTGGACTCA GCCTCGGTGA ACTCCATCTC GTCCATACCC TCACCGGTGT ACCAGTGCAG
          301 GAAAGCCTTG CGGCGGAACA TAGCGGTGAA CTGCTCGGAG ATGCGCTTGA ACAGCTCCTG
          361 GATAGCGGTG GAGTTACCGA TGAAGGTGGA GGACATCTTC AGACCGCGAG GAGGGATGTC
          421 GCACACAGCC ACCTTCACGT TGTTAGGGAT CCACTCCACG AAGTAGGAGG AGTTCTTGGA
          481 CTGGATAGCC AGCATCTGCT CGTCCACCTC CTTCATGGAC ATGCGACCGC GGAACACGGT
          541 AGCCACGGTC AGGTAGCGAC CGTGGCGAGG GTCGCAAGCA GCCATCATGT TCTTAGCGTC
          601 GAACATCTGC TGGGTCAGCT CAGGCACGGT CAGAGCGCGG TATTGCTGGG AACCGCGAGC
          661 GGTCAGAGGA GCGAAACCAG GCATGAAGAA GTGCAGGCGA GGGAAAGGCA CCATGTTCAC
          721 AGCCAGCTTG CGCAGGTCAG CGTTCAGCTG ACCAGGGAAG CGCAGGGAGG TGGTCACACC
          781 GGACATGGTA GCGGAGACCA GGTGGTTCAG GTCACCGTAG GTAGGGGTAG CCAGCTTCAG
          841 GGTGCGGAAG CAGATGTCGT ACAGAGCCTC GTTGTCGATG CAGTAGGTCT CGTCGGTGTT
          901 CTCCACCAGT TGGTGGATGG ACAGGGTAGC GTTGTAAAGG CTCCACCACG GGTGTCGGAC
          961 ATCCTTAGGG GAAAGGCACG ACGGAGAAGG TATTCATGAT GCGGGTCAAG GTTACTCCCT
         1021 CGCGCACCCT TGAGATCAGC AGGTTACCCC ATACCAGAAT CCGGGTACCA CCACCCAGGA
         1081 GTGAAGTCAG CTGGGAAAAC CCCTGCAGGC AGTCGCAGTT CTCGCACCTC TTGCGCACCA
        1141 CGTCAGCACG GAGTCGACGA GCCTCAGCAA CCCCTTCCGG TGTTTAGAGT GAAATC
        //
        Primer: SV40pA3'element Reverse
        Region: Tn7R,GmR
        Sequence file: RDB12754_A4Dfe.seq check
        >12754_12754_A9IhF_SV40pA3'elementReverse_G02_07_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
           61 TTGCTCNCAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
          121 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG
          181 AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC
          241 ACCGCATAGA CCAGCCGCGT AACCTGGCAA AATCGGTTAC GGTTGAGTAA TAAATGGATG
          301 CCCTGCGTAA GCGGGTGTGG GCGGACAATA AAGTCTTAAA CTGAACAAAA TAGATCTAAA
          361 CTATGACAAT AAAGTCTTAA ACTAGACAGA ATAGTTGTAA ACTGAAATCA GTCCAGTTAT
          421 GCTGTGAAAA AGCATACTGG ACTTTTGTTA TGGCTAAAGC AAACTCTTCA TTTTCTGAAG
          481 TGCAAATTGC CCGTCGTATT AAAGAGGGGC GTGGCCAAGG GCATGGTAAA GACTATATTC
          541 GCGGCGTTGT GACAATTTAC CGAACAACTC CGCGGCCGGG AAGCCGATCT CGGCTTGAAC
          601 GAATTGTTAG GTGGCGGTAC TTGGGTCGAT ATCAAAGTGC ATCACTTCTT CCCGTATGCC
        661 CAACTTTGNN
        //
        Primer: f1ori_R3
        Region: Tn7L
        Sequence file: RDB12754_A4Dff.seq check
        >12754_12754_A9IhF_f1ori_R3_A04_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNATTACNN ACNTTAATAA CGAAGNAGNN GACAGAAAAA
           61 TTTTCATTCT GTGACAGAGA AAAAGTAGCC GAAGATGACG GTTTGTCACA TGGAGTTGGC
          121 AGGATGTTTG ATTAAAAACA TAACAGGAAG AAAAATGCCC CGCTGTGGGC GGACAAAATA
        181 GTTGGGAACT GGGAGGGGTG GAAATGGAGT TTTTAAGGAT NNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl