Resource data sheet
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pFBD-Hs alpha1(K40R)CGH/beta3CGF

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin

Catalog number RDB12754
Resource name pFBD-Hs alpha1(K40R)CGH/beta3CGF
Alternative name FLAG-and His-tagged Human alpha1 beta3 tubulin(K40R)
Clone info. Plasmid clone for baculoviral expression of alpha1(NP_006073) and beta3 (NP_006077) tubulin. K40R mutant form.
Human alpha1-tubulin, in which lysine 40 is substituted with arginine, is fused to linker (GGSGG) and His-tag (HHHHHHHH) at its C-terminus. Human b3-tubulin is fused to linker (GGSGG) and FLAG-tag (DYKDDDDK) at its C-terminus.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Human alpha1-Tb cDNA Human eta3-Tb cDNA
Depositor Muto, Etsuko |
 
Other clones in our bank human TUBA1B (NCBI Gene 10376) | human TUBB3 (NCBI Gene 10381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB12754 pFBD-Hs alpha1(K40R)CGH/beta3CGF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Purification of Recombinant Tubulin (pdf)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12754_A4Dfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: polyhedrin pro, L21, insert2-5' | Primer: pPolyhedrin_F | Sequence: RDB12754_A4Dfa.seq
>12754_3_pPolyhedrin_F_E05_14.ab1
    1 CAATTTTTTA CTTGTTTTCG TAACAGTTTT GTAATAAAAA AACCTATAAA TATTCCGGAT
   61 TATTCATACC GTCCCACCAT CGGGCGCGGA TCCCGGTCCG AAGCGCGCGG AATTCAAAGG
  121 CCTACGTCGA CGAGCTCACT AGTAACTCCT AAAAAACCGC CACCATGCGC GAGTGCATCT
  181 CCATCCACGT GGGCCAGGCT GGTGTCCAGA TCGGCAACGC TTGCTGGGAG CTGTACTGCC
  241 TGGAGCACGG TATCCAGCCC GACGGCCAGA TGCCCTCCGA CCGCACCATC GGTGGCGGCG
  301 ACGACTCCTT CAACACCTTC TTCTCCGAAA CCGGTGCTGG CAAGCACGTG CCCCGCGCTG
  361 TGTTCGTGGA TCTGGAACCC ACCGTGATCG ACGAAGTGCG TACCGGCACC TACCGTCAGC
  421 TGTTCCACCC CGAGCAACTG ATCACCGGCA AGGAGGACGC TGCTAACAAC TACGCTCGTG
  481 GTCACTACAC CATCGGCAAG GAAATCATCG ACCTGGTCCT GGACCGTATC CGCAAGCTGG
  541 CTGACCAGTG CACCGGCCTG CAGGGATTCC TCGTGTTCCA CTCCTTCGGT GGTGGAACCG
  601 GTTCTGGTTT CACCTCCCTG CTGATGGAAC GTCTGTCTGT GGACTACGGC AAGAAGTCCA
  661 AGTTGGAGTT CTCCATCTAC CCCGCTCCCC AGGTGTCCAC CGCTGTGGTC GAGCCCTACA
  721 ACTCCATCCT GACCACCCAC ACCACCCTGG AACACTCCGA CTGCGCTTTC ATGGTGGACA
  781 ACGAGGCTAT CTACGACATC TGCCGTCGTA ACCTGGACAT CGAGCGTCCC ACCTACACCA
  841 ACCTGAACCG TCTGATCTCC CAGATCGTGT CCTCCATCAC CGCTTCCCTG CGTTTCGACG
  901 GTGCTCTGAA CGTGGACCTG ACCGAGTTCC AGACCAACCT GGTGCCCTAC CCCCGTATCC
  961 ACTTCCCCCT GGCTACCTAC GCTCCCGTGA TCTCCGCTGA GAAGCTTACC ACGAGCAGCT
 1021 GTCCGTGGGC TGAGATCACC AAACGCCTGC TTCGAGCCCG CTAACAGATG GTCAAGTGCG
 1081 ACCCCCCGTC ACGGCCAGTA CATGGCTTGC TGCCTTGCTG TACGTGCGAA CGTGGTGGCT
1141 AGGACGTGAC GGCTGCCTAT TCGGCTACCA TCTCAAGAAA CTA
//
Region: 8xHis,GGSGG, insert2-3' | Primer: ME-1290Rv | Sequence: RDB12754_A4Dfb.seq
>12754_3_1290Rv_F05_17.ab1
    1 CTGTCCATTT CATTTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
   61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCC TCTAGTACTT CTCGACAAGC
  121 TTTTAGTGGT GATGGTGGTG ATGATGGTGA CCACCGGAAC CACCGTATTC CTCGCCTTCC
  181 TCTTCGCCCT CGCCCTCGAC GGAGTCGACG CCCACTTCCT CGTAGTCCTT TTCCAGAGCA
  241 GCCATGTCCT CGCGAGCCTC GGAGAACTCG CCCTCTTCCA TGCCTTCGCC CACGTACCAG
  301 TGCACGAAAG CGCGCTTAGC GTACATCAGG TCGAACTTGT GGTCCAGACG AGCCCAAGCC
  361 TCAGCGATAG CGGTGGTGTT GGACAGCATG CACACAGCAC GCTGCACCTT AGCCAAGTCG
  421 CCACCGGGCA CCACAGTGGG GGGCTGGTAG TTGATTCCGA CCTTGAAACC GGTGGGGCAC
  481 CAGTCCACGA ACTGGATGGA ACGCTTGGTC TTGATGGTAG CGATAGCAGC GTTCACGTCC
  541 TTAGGCACCA CGTCGCCACG GTACAGCAGG CAGCAAGCCA TGTACTTGCC GTGACGGGGG
  601 TCGCACTTGA CCATCTGGTT AGCGGGCTCG AAGCAGGCGT TGGTGATCTC AGCCACGGAC
  661 AGCTGCTCGT GGTAAGCCTT CTCAGCGGAG ATCACGGGAG CGTAGGTAGC CAGGGGGAAG
  721 TGGATACGGG GGTAGGGCAC CAGGTTGGTC TGGAACTCGG TCAGGTCCAC GTTCAGAGCA
  781 CCGTCGAAAC GCAGGGAAGC GGTGATGGAG GACACGATCT GGGAGATCAG ACGGTTCAGG
  841 TTGGTGTAGG TGGGACGCTC GATGTCCAGG TTACGACGGC AGATGTCGTA GATAGCCTCG
  901 TTGTCCACCA TGAAAGCGCA GTCGGAGTGT TCCAGGGTGG TGTGGGTGGT CAGGATGGAG
  961 TTGTAGGGGC TCGACCACAG CGGTGGACAC CTGGGGAGCG GGGTAGATGG AGAACTCCTA
 1021 CTTTGGACTT CTGCCGTAGT CCACAGACAG ACGTTCCAAT CAGCAGGGAG GTGAAACCAG
 1081 AACTGATCAC AACGAAGAGT GTACACGAGG ATTCCTGCAG GCTGTTGCAC TGTCCAGGCC
1141 GAGCTTGGCC GGGAATATAC G
//
Region: p10pro,21L,insert1-5' | Primer: pP10_F | Sequence: RDB12754_A4Dfc.seq
>12754_3_pP10_F_G05_20.ab1
    1 TTTAAATAAG GAATTTATTT ATCAAATCAT TTGTATATTA ATTAAAATAC TATACTGTAA
   61 ATTACATTTT ATTTACAATC ACTCGACGAA GACTTGATCA CCCGGGATCT CGAGAACTCC
  121 TAAAAAACCG CCACCATGCG CGAGATCGTG CACATCCAGG CTGGTCAGTG CGGTAACCAG
  181 ATCGGTGCTA AGTTCTGGGA GGTCATCTCC GACGAGCACG GTATCGACCC TTCCGGTAAC
  241 TACGTGGGTG ACTCCGACCT GCAGCTGGAG CGCATTTCCG TGTACTACAA CGAAGCCTCC
  301 TCCCACAAAT ACGTGCCTCG CGCTATCCTG GTGGACCTGG AGCCTGGTAC TATGGACTCT
  361 GTGCGCTCCG GTGCTTTCGG TCACCTGTTC CGCCCTGACA ACTTCATCTT CGGTCAGTCC
  421 GGTGCTGGTA ACAACTGGGC TAAGGGTCAC TACACCGAGG GTGCTGAGCT CGTCGACTCC
  481 GTGCTGGACG TGGTGCGCAA GGAGTGCGAG AACTGCGACT GCCTGCAGGG TTTCCAGCTG
  541 ACTCACTCCC TGGGTGGTGG TACCGGATCT GGTATGGGTA CCCTGCTGAT CTCCAAGGTG
  601 CGCGAGGAGT ACCCTGACCG CATCATGAAT ACCTTCTCCG TCGTGCCTTC CCCTAAGGTG
  661 TCCGACACCG TGGTGGAGCC TTACAACGCT ACCCTGTCCA TCCACCAACT GGTGGAGAAC
  721 ACCGACGAGA CCTACTGCAT CGACAACGAG GCTCTGTACG ACATCTGCTT CCGCACCCTG
  781 AAGCTGGCTA CCCCTACCTA CGGTGACCTG AACCACCTGG TCTCCGCTAC CATGTCCGGT
  841 GTGACCACCT CCCTGCGCTT CCCTGGTCAG CTGAACGCTG ACCTGCGCAA AGCTGGCTGT
  901 GAACATGGTG CCTTTTCCCT CGCCTGCACT TCTTTCATGC CTGGTTTCGC TCCTCTGACC
  961 GCTCGCGGTT CCCCAGCAAT TACCGCGCTC TGACCGTGCC CTGAGCTGAC CCAGCCAGAA
 1021 TGTTCGACGC TAAGAACATG ATGGCTGCTT GCGACCCTCG GCCACCGGTC GGCTACCTGA
 1081 ACCGGTGGCC TACCGTGGTT CCGCGGGTCG GCATGTCATG AAGGAAGGTT GGACGAAGCA
 1141 GATGGCCTGG CTATCAGTCC AGGAAACCTC CCTCCTACCT TTCCGCTGGG GAAGATTGTG
1201 AAAATCCCCT TAACACAC
//
Region: FLAG,GGSGG,insert1-3' | Primer: HSV_tk_pA_R | Sequence: RDB12754_A4Dfd.seq
>12754_3_HSV_tk_pA_R_H05_23.ab1
    1 AGGGGGTCCT TTCCGGTATT GTCTTCTTCC GTGTTTCAGG TTAAGCCTCC CCCATCTCCC
   61 GGTACCGCAT GCTATGCATC AGCTGCTAGC TTACTTGTCA TCGTCATCTT TGTAGTCACC
  121 ACCGGAACCA CCCTTAGGAC CCTGAGCCTC GGACTCTTCC TCATCGTCCT CGTACATCTC
  181 ACCCTCTTCT TCAGCGGTAG CGTCCTGGTA CTGCTGGTAC TCGGACACCA GGTCGTTCAT
  241 GTTGGACTCA GCCTCGGTGA ACTCCATCTC GTCCATACCC TCACCGGTGT ACCAGTGCAG
  301 GAAAGCCTTG CGGCGGAACA TAGCGGTGAA CTGCTCGGAG ATGCGCTTGA ACAGCTCCTG
  361 GATAGCGGTG GAGTTACCGA TGAAGGTGGA GGACATCTTC AGACCGCGAG GAGGGATGTC
  421 GCACACAGCC ACCTTCACGT TGTTAGGGAT CCACTCCACG AAGTAGGAGG AGTTCTTGGA
  481 CTGGATAGCC AGCATCTGCT CGTCCACCTC CTTCATGGAC ATGCGACCGC GGAACACGGT
  541 AGCCACGGTC AGGTAGCGAC CGTGGCGAGG GTCGCAAGCA GCCATCATGT TCTTAGCGTC
  601 GAACATCTGC TGGGTCAGCT CAGGCACGGT CAGAGCGCGG TATTGCTGGG AACCGCGAGC
  661 GGTCAGAGGA GCGAAACCAG GCATGAAGAA GTGCAGGCGA GGGAAAGGCA CCATGTTCAC
  721 AGCCAGCTTG CGCAGGTCAG CGTTCAGCTG ACCAGGGAAG CGCAGGGAGG TGGTCACACC
  781 GGACATGGTA GCGGAGACCA GGTGGTTCAG GTCACCGTAG GTAGGGGTAG CCAGCTTCAG
  841 GGTGCGGAAG CAGATGTCGT ACAGAGCCTC GTTGTCGATG CAGTAGGTCT CGTCGGTGTT
  901 CTCCACCAGT TGGTGGATGG ACAGGGTAGC GTTGTAAAGG CTCCACCACG GGTGTCGGAC
  961 ATCCTTAGGG GAAAGGCACG ACGGAGAAGG TATTCATGAT GCGGGTCAAG GTTACTCCCT
 1021 CGCGCACCCT TGAGATCAGC AGGTTACCCC ATACCAGAAT CCGGGTACCA CCACCCAGGA
 1081 GTGAAGTCAG CTGGGAAAAC CCCTGCAGGC AGTCGCAGTT CTCGCACCTC TTGCGCACCA
1141 CGTCAGCACG GAGTCGACGA GCCTCAGCAA CCCCTTCCGG TGTTTAGAGT GAAATC
//
Region: Tn7R,GmR | Primer: SV40pA3'element Reverse | Sequence: RDB12754_A4Dfe.seq
>12754_12754_A9IhF_SV40pA3'elementReverse_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
   61 TTGCTCNCAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
  121 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG
  181 AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC
  241 ACCGCATAGA CCAGCCGCGT AACCTGGCAA AATCGGTTAC GGTTGAGTAA TAAATGGATG
  301 CCCTGCGTAA GCGGGTGTGG GCGGACAATA AAGTCTTAAA CTGAACAAAA TAGATCTAAA
  361 CTATGACAAT AAAGTCTTAA ACTAGACAGA ATAGTTGTAA ACTGAAATCA GTCCAGTTAT
  421 GCTGTGAAAA AGCATACTGG ACTTTTGTTA TGGCTAAAGC AAACTCTTCA TTTTCTGAAG
  481 TGCAAATTGC CCGTCGTATT AAAGAGGGGC GTGGCCAAGG GCATGGTAAA GACTATATTC
  541 GCGGCGTTGT GACAATTTAC CGAACAACTC CGCGGCCGGG AAGCCGATCT CGGCTTGAAC
  601 GAATTGTTAG GTGGCGGTAC TTGGGTCGAT ATCAAAGTGC ATCACTTCTT CCCGTATGCC
661 CAACTTTGNN
//
Region: Tn7L | Primer: f1ori_R3 | Sequence: RDB12754_A4Dff.seq
>12754_12754_A9IhF_f1ori_R3_A04_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNATTACNN ACNTTAATAA CGAAGNAGNN GACAGAAAAA
   61 TTTTCATTCT GTGACAGAGA AAAAGTAGCC GAAGATGACG GTTTGTCACA TGGAGTTGGC
  121 AGGATGTTTG ATTAAAAACA TAACAGGAAG AAAAATGCCC CGCTGTGGGC GGACAAAATA
181 GTTGGGAACT GGGAGGGGTG GAAATGGAGT TTTTAAGGAT NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

2019.10.11

GNP_filter3_RDBDEP_html_190927.pl