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pFBD-Hs alpha1CGH/beta3CGF

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin

Catalog number RDB12753
Resource name pFBD-Hs alpha1CGH/beta3CGF
Alternative name FLAG-and His-tagged Human alpha1 beta3 tubulin
Clone info. Plasmid clone for baculoviral expression of alpha1(NP_006073) and beta3 (NP_006077) tubulin.
Comment Human alpha1-tubulin is fused to linker (GGSGG) and His-tag (HHHHHHHH) at its C-terminus. Human b3-tubulin is fused to linker (GGSGG) and FLAG-tag (DYKDDDDK) at its C-terminus.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Human alpha1-Tb cDNA Human beta3-Tb cDNA
Depositor|Developer Muto, Etsuko |
Other clones in our bank

External Database
Human alpha1-Tb Human beta3-Tb

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (FEBS Lett., 587, 3450-3455 (2013))を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB12753 pFBD-Hs alpha1CGH/beta3CGF DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Remarks, protocol and/or map (pdf) RDB12753z.pdf

        Original reference

        original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Purification of Recombinant Tubulin (pdf)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB12753_A5Ftp1-2 check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pPolyhedrin_F
        Sequence file: RDB12753_A5Fta.seq check
        >12753_12753_A5Ft_pPolyhedrin_F_B06_06.ab1
            1 ATTTTTACTG TTTTCGTAAC AGTTTTGTAA TAAAAAAACC TATAAATATT CCGGATTATT
           61 CATACCGTCC CACCATCGGG CGCGGATCCC GGTCCGAAGC GCGCGGAATT CAAAGGCCTA
          121 CGTCGACGAG CTCACTAGTA ACTCCTAAAA AACCGCCACC ATGCGCGAGT GCATCTCCAT
          181 CCACGTGGGC CAGGCTGGTG TCCAGATCGG CAACGCTTGC TGGGAGCTGT ACTGCCTGGA
          241 GCACGGTATC CAGCCCGACG GCCAGATGCC CTCCGACAAG ACCATCGGTG GCGGCGACGA
          301 CTCCTTCAAC ACCTTCTTCT CCGAAACCGG TGCTGGCAAG CACGTGCCCC GCGCTGTGTT
          361 CGTGGATCTG GAACCCACCG TGATCGACGA AGTGCGTACC GGCACCTACC GTCAGCTGTT
          421 CCACCCCGAG CAACTGATCA CCGGCAAGGA GGACGCTGCT AACAACTACG CTCGTGGTCA
          481 CTACACCATC GGCAAGGAAA TCATCGACCT GGTCCTGGAC CGTATCCGCA AGCTGGCTGA
          541 CCAGTGCACC GGCCTGCAGG GATTCCTCGT GTTCCACTCC TTCGGTGGTG GAACCGGTTC
          601 TGGTTTCACC TCCCTGCTGA TGGAACGTCT GTCTGTGGAC TACGGCAAGA AGTCCAAGTT
          661 GGAGTTCTCC ATCTACCCCG CTCCCCAGGT GTCCACCGCT GTGGTCGAGC CCTACAACTC
          721 CATCCTGACC ACCCACACCA CCCTGGAACA CTCCGACTGC GCTTTCATGG TGGACAACGA
          781 GGCTATCTAC GACATCTGCC GTCGTAACCT GGACATCGAG CGTCCCACCT ACACCAACCT
          841 GAACCGTCTG ATCTCCCAGA TCGTGTCCTC CATCACCGCT TCCCTGCGTT TCGACGGTGC
          901 TCTGAACGTG GACCTGACCG AGTTCCAGAC CAACCTGGTG CCCTACCCCC GTATCCACTT
          961 CCCCCTGGCT ACCTACGCTC CCGTGATCTC CGCTGAGAAG GCTTACCACG AGCAGCTGTC
         1021 CGTGGCTGAG ATCACCAAAC GCCTGCTTCG AGCCCGCTAA CCAGATGGTC AAGTGGCGAC
         1081 CCCCCGTCAC GGCAGTACAT ACTTGCTGCC TGCCTGTAAC GTTACCGACG TTGATTGCTA
        1141 AGACGTGAAC GGCTGCTATT CGGCCCTTAC CATCCAAGGA ACCTCCAAG
        //
        Primer: ME-1290RV
        Sequence file: RDB12753_A5Ftb.seq check
        >12753_12753_A5Ft_ME-1290Rv_C06_09.ab1
            1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
           61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT AGTACTTCTC GACAAGCTTT
          121 TAGTGGTGAT GGTGGTGATG ATGGTGACCA CCGGAACCAC CGTATTCCTC GCCTTCCTCT
          181 TCGCCCTCGC CCTCGACGGA GTCGACGCCC ACTTCCTCGT AGTCCTTTTC CAGAGCAGCC
          241 ATGTCCTCGC GAGCCTCGGA GAACTCGCCC TCTTCCATGC CTTCGCCCAC GTACCAGTGC
          301 ACGAAAGCGC GCTTAGCGTA CATCAGGTCG AACTTGTGGT CCAGACGAGC CCAAGCCTCA
          361 GCGATAGCGG TGGTGTTGGA CAGCATGCAC ACAGCACGCT GCACCTTAGC CAAGTCGCCA
          421 CCGGGCACCA CAGTGGGGGG CTGGTAGTTG ATTCCGACCT TGAAACCGGT GGGGCACCAG
          481 TCCACGAACT GGATGGAACG CTTGGTCTTG ATGGTAGCGA TAGCAGCGTT CACGTCCTTA
          541 GGCACCACGT CGCCACGGTA CAGCAGGCAG CAAGCCATGT ACTTGCCGTG ACGGGGGTCG
          601 CACTTGACCA TCTGGTTAGC GGGCTCGAAG CAGGCGTTGG TGATCTCAGC CACGGACAGC
          661 TGCTCGTGGT AAGCCTTCTC AGCGGAGATC ACGGGAGCGT AGGTAGCCAG GGGGAAGTGG
          721 ATACGGGGGT AGGGCACCAG GTTGGTCTGG AACTCGGTCA GGTCCACGTT CAGAGCACCG
          781 TCGAAACGCA GGGAAGCGGT GATGGAGGAC ACGATCTGGG AGATCAGACG GTTCAGGTTG
          841 GTGTAGGTGG GACGCTCGAT GTCCAGGTTA CGACGGCAGA TGTCGTAGAT AGCCTCGTTG
        901 TCCACCATGA AAGCGCAGTC GGGAGTGTTC CAGGGTGGTG TGGGTGGTTC AGGATGGG
        //
        Primer: pP10_F
        Sequence file: RDB12753_A5Ftc.seq check
        >12753_12753_A5Ft_pP10_F_D06_12.ab1
            1 TTATAGAATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT
           61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA
          121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG
          181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG
          241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA
          301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT
          361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG
          421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG
          481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT
          541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG
          601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA
          661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG
          721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG
          781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA
          841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG
          901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGTTTCGC TCCTCTGACC GCTCGCGGTT
          961 CCCAGCAATA CCGCGCTCTG ACCGTGCCTG AGCTGACCCA GCAGATGTTC GACGCTAAGA
         1021 ACATGATGGC TGCTTGCGAC CCTCGCCACG GGTCGCTACC TGACGTGGCT ACGTGTCCGC
         1081 GGTCGCATGT CCATGATGAG TGGACGAGCA GATGCTGCTA TCAGTTCAGG ACTCTCCTAC
        1141 ATCGTTGGAG TGCAATCCTA ACCAAACGGT GGAAAAAG
        //
        Primer: HSV_TK_pA_R
        Sequence file: RDB12753_A5Ftd.seq check
        >12753_12753_A5Ft_HSVTKpA_R_E06_15.ab1
            1 ACGGTCTTCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCATCT CCCGGTACCG
           61 CATGCTATGC ATCAGCTGCT AGCTTACTTG TCATCGTCAT CTTTGTAGTC ACCACCGGAA
          121 CCACCCTTAG GACCCTGAGC CTCGGACTCT TCCTCATCGT CCTCGTACAT CTCACCCTCT
          181 TCTTCAGCGG TAGCGTCCTG GTACTGCTGG TACTCGGACA CCAGGTCGTT CATGTTGGAC
          241 TCAGCCTCGG TGAACTCCAT CTCGTCCATA CCCTCACCGG TGTACCAGTG CAGGAAAGCC
          301 TTGCGGCGGA ACATAGCGGT GAACTGCTCG GAGATGCGCT TGAACAGCTC CTGGATAGCG
          361 GTGGAGTTAC CGATGAAGGT GGAGGACATC TTCAGACCGC GAGGAGGGAT GTCGCACACA
          421 GCCACCTTCA CGTTGTTAGG GATCCACTCC ACGAAGTAGG AGGAGTTCTT GGACTGGATA
          481 GCCAGCATCT GCTCGTCCAC CTCCTTCATG GACATGCGAC CGCGGAACAC GGTAGCCACG
          541 GTCAGGTAGC GACCGTGGCG AGGGTCGCAA GCAGCCATCA TGTTCTTAGC GTCGAACATC
          601 TGCTGGGTCA GCTCAGGCAC GGTCAGAGCG CGGTATTGCT GGGAACCGCG AGCGGTCAGA
          661 GGAGCGAAAC CAGGCATGAA GAAGTGCAGG CGAGGGAAAG GCACCATGTT CACAGCCAGC
          721 TTGCGCAGGT CAGCGTTCAG CTGACCAGGG AAGCGCAGGG AGGTGGTCAC ACCGGACATG
          781 GTAGCGGAGA CCAGGTGGTT CAGGTCACCG TAGGTAGGGG TAGCCAGCTT CAGGGTGCGG
          841 AAGCAGATGT CGTACAGAGC CTCGTTGTCG ATGCAGTAGG TCTCGTCGGT GTTCTCCACC
          901 AGTTGGTGGA TGGACAGGGT AGCGTTGTAA AGCTCCACCA CGGTGTCGGA CACCTTATGG
          961 GAAGGGCACG ACGGAGAAGG TATTCATGAT GCGGTCAGGG TACTCCTCGC GCACCTTGGA
         1021 GATCAGCAGG TACCCATACC AGATCCGGTA CCACCAACCC AGGGAGTGAG TCAGCTGGAA
         1081 AACCCCTGCA GCAGTCGCAG TCTCGCCACT CCTTGCGCAC ACGTTCAGCA CGAGTTCGAA
        1141 CGAGCTCAGC ACCCCTCTCG GGTGTTAGTG AACCCCTTAT AGTCCCCAAG T
        //
        Primer: pBACe3.6_F
        Sequence file: RDB12753_A5Fte.seq check
        >12753_12753_A5Ft_pBACe3.6_F_F06_18.ab1
            1 TGTAGCCTGC GTAGCGGGTG TGGGCGGACA ATAAAGTCTT AAACTGAACA AAATAGATCT
           61 AAACTATGAC AATAAAGTCT TAAACTAGAC AGAATAGTTG TAAACTGAAA TCAGTCCAGT
          121 TATGCTGTGA AAAAGCATAC TGGACTTTTG TTATGGCTAA AGCAAACTCT TCATTTTCTG
          181 AAGTGCAAAT TGCCCGTCGT ATTAAAGAGG GGCGTGGCCA AGGGCATGGT AAAGACTATA
          241 TTCGCGGCGT TGTGACAATT TACCGAACAA CTCCGCGGCC GGGAAGCCGA TCTCGGCTTG
          301 AACGAATTGT TAGGTGGCGG TACTTGGGTC GATATCAAAG TGCATCACTT CTTCCCGTAT
          361 GCCCAACTTT GTATAGAGAG CCACTGCGGG ATCGTCACCG TAATCTGCTT GCACGTAGAT
          421 CACATAAGCA CCAAGCGCGT TGGCCTCATG CTTGAGGAGA TTGATGAGCG CGGTGGCAAT
          481 GCCCTGCCTC CGGTGCTCGC CGGAGACTGC GAGATCATAG ATATAGATCT CACTACGCGG
          541 CTGCTCAAAC TTGGGCAGAA CGTAAGCCGC GAGAGCGCCA ACAACCGCTT CTTGGTCGAA
          601 GGCAGCAAGC GCGATGAATG TCTTACTACG GAGCAAGTTC CCGAGGTAAT CGGAGTCCGG
          661 CTGATGTTGG GAGTAGGTGG CTACGTCTCC GAACTCACGA CCGAAAAGAT CAAGAGCAGC
          721 CCGCATGGAT TTGACTTGGT CAGGGCCGAG CCTACATGTG CGAATGATGC CCATACTTGA
          781 GCCACCTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTGCGTAA
          841 CATCGTTGCT GCTCCATAAC ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG
          901 ATGCCCGAGG CATAGACTGT ACAAAAAAAC AGTCATAACA AGCCATGAAA ACCGCCACTG
          961 CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG CGCATACGCT
         1021 ACTTGCATTA CAGTTTACGA CCGAACAGCT TATGTCACTG GGATCGTGCT CATCGTTTCA
         1081 CGGTGTGCGT CACCGCCAGT GGCAGCAGCG AGTCGAGGCA TTCCTGTCGT GGTCTGCGAC
        1141 CGAGCGGCCA AGGATTCGGT CTTCCAACGG
        //
        Primer: pGCAP_R
        Sequence file: RDB12753_A5Ftf.seq check
        >12753_12753_A5Ft_pGCAP_R_G06_21.ab1
            1 AGAATCTTAT CATGTCTGGA TCTGATCACT GCTTGAGCCT AGGAGATCCG AACCAGATAA
           61 GTGAAATCTA GTTCCAAACT ATTTTGTCAT TTTTAATTTT CGTATTAGCT TACGACGCTA
          121 CACCCAGTTC CCATCTATTT TGTCACTCTT CCCTAAATAA TCCTTAAAAA CTCCATTTCC
          181 ACCCCTCCCA GTTCCCAACT ATTTTGTCCG CCCACAGCGG GGCATTTTTC TTCCTGTTAT
          241 GTTTTTAATC AAACATCCTG CCAACTCCAT GTGACAAACC GTCATCTTCG GCTACTTTTT
          301 CTCTGTCACA GAATGAAAAT TTTTCTGTCA TCTCTTCGTT ATTAATGTTT GTAATTGACT
          361 GAATATCAAC GCTTATTTGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT
          421 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
          481 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
          541 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
          601 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
          661 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
          721 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
          781 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
          841 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
          901 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT
          961 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTTATTCC
         1021 CTTTTTTGCG GCATTTTGCC TTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAGTAAA
         1081 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTACATCGA CTGGATCTCA CAGCGTAGGA
        1141 TTCATGAAGT TTTCGCTCTC GAGAACGGTT TTTCCAATGA TGGAGAGCAC AC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl