Resource data sheet
Prev.

pFBD-Hs alpha1CGH/beta3CGF

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin

Catalog number RDB12753
Resource name pFBD-Hs alpha1CGH/beta3CGF
Alternative name FLAG-and His-tagged Human alpha1 beta3 tubulin
Clone info. Plasmid clone for baculoviral expression of alpha1(NP_006073) and beta3 (NP_006077) tubulin.
Human alpha1-tubulin is fused to linker (GGSGG) and His-tag (HHHHHHHH) at its C-terminus. Human b3-tubulin is fused to linker (GGSGG) and FLAG-tag (DYKDDDDK) at its C-terminus.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Human alpha1-Tb cDNA Human eta3-Tb cDNA
Depositor Muto, Etsuko |
 
Other clones in our bank human TUBA1B (NCBI Gene 10376) | human TUBB3 (NCBI Gene 10381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12753 pFBD-Hs alpha1CGH/beta3CGF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB12753z.pdf from Depositor

Featured content

Featured content Purification of Recombinant Tubulin (pdf)

References

original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12753_A5Ftp1-2

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F RDB12753_A5Fta.seq
>12753_12753_A5Ft_pPolyhedrin_F_B06_06.ab1
    1 ATTTTTACTG TTTTCGTAAC AGTTTTGTAA TAAAAAAACC TATAAATATT CCGGATTATT
   61 CATACCGTCC CACCATCGGG CGCGGATCCC GGTCCGAAGC GCGCGGAATT CAAAGGCCTA
  121 CGTCGACGAG CTCACTAGTA ACTCCTAAAA AACCGCCACC ATGCGCGAGT GCATCTCCAT
  181 CCACGTGGGC CAGGCTGGTG TCCAGATCGG CAACGCTTGC TGGGAGCTGT ACTGCCTGGA
  241 GCACGGTATC CAGCCCGACG GCCAGATGCC CTCCGACAAG ACCATCGGTG GCGGCGACGA
  301 CTCCTTCAAC ACCTTCTTCT CCGAAACCGG TGCTGGCAAG CACGTGCCCC GCGCTGTGTT
  361 CGTGGATCTG GAACCCACCG TGATCGACGA AGTGCGTACC GGCACCTACC GTCAGCTGTT
  421 CCACCCCGAG CAACTGATCA CCGGCAAGGA GGACGCTGCT AACAACTACG CTCGTGGTCA
  481 CTACACCATC GGCAAGGAAA TCATCGACCT GGTCCTGGAC CGTATCCGCA AGCTGGCTGA
  541 CCAGTGCACC GGCCTGCAGG GATTCCTCGT GTTCCACTCC TTCGGTGGTG GAACCGGTTC
  601 TGGTTTCACC TCCCTGCTGA TGGAACGTCT GTCTGTGGAC TACGGCAAGA AGTCCAAGTT
  661 GGAGTTCTCC ATCTACCCCG CTCCCCAGGT GTCCACCGCT GTGGTCGAGC CCTACAACTC
  721 CATCCTGACC ACCCACACCA CCCTGGAACA CTCCGACTGC GCTTTCATGG TGGACAACGA
  781 GGCTATCTAC GACATCTGCC GTCGTAACCT GGACATCGAG CGTCCCACCT ACACCAACCT
  841 GAACCGTCTG ATCTCCCAGA TCGTGTCCTC CATCACCGCT TCCCTGCGTT TCGACGGTGC
  901 TCTGAACGTG GACCTGACCG AGTTCCAGAC CAACCTGGTG CCCTACCCCC GTATCCACTT
  961 CCCCCTGGCT ACCTACGCTC CCGTGATCTC CGCTGAGAAG GCTTACCACG AGCAGCTGTC
 1021 CGTGGCTGAG ATCACCAAAC GCCTGCTTCG AGCCCGCTAA CCAGATGGTC AAGTGGCGAC
 1081 CCCCCGTCAC GGCAGTACAT ACTTGCTGCC TGCCTGTAAC GTTACCGACG TTGATTGCTA
1141 AGACGTGAAC GGCTGCTATT CGGCCCTTAC CATCCAAGGA ACCTCCAAG
//
Primer: ME-1290RV RDB12753_A5Ftb.seq
>12753_12753_A5Ft_ME-1290Rv_C06_09.ab1
    1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT AGTACTTCTC GACAAGCTTT
  121 TAGTGGTGAT GGTGGTGATG ATGGTGACCA CCGGAACCAC CGTATTCCTC GCCTTCCTCT
  181 TCGCCCTCGC CCTCGACGGA GTCGACGCCC ACTTCCTCGT AGTCCTTTTC CAGAGCAGCC
  241 ATGTCCTCGC GAGCCTCGGA GAACTCGCCC TCTTCCATGC CTTCGCCCAC GTACCAGTGC
  301 ACGAAAGCGC GCTTAGCGTA CATCAGGTCG AACTTGTGGT CCAGACGAGC CCAAGCCTCA
  361 GCGATAGCGG TGGTGTTGGA CAGCATGCAC ACAGCACGCT GCACCTTAGC CAAGTCGCCA
  421 CCGGGCACCA CAGTGGGGGG CTGGTAGTTG ATTCCGACCT TGAAACCGGT GGGGCACCAG
  481 TCCACGAACT GGATGGAACG CTTGGTCTTG ATGGTAGCGA TAGCAGCGTT CACGTCCTTA
  541 GGCACCACGT CGCCACGGTA CAGCAGGCAG CAAGCCATGT ACTTGCCGTG ACGGGGGTCG
  601 CACTTGACCA TCTGGTTAGC GGGCTCGAAG CAGGCGTTGG TGATCTCAGC CACGGACAGC
  661 TGCTCGTGGT AAGCCTTCTC AGCGGAGATC ACGGGAGCGT AGGTAGCCAG GGGGAAGTGG
  721 ATACGGGGGT AGGGCACCAG GTTGGTCTGG AACTCGGTCA GGTCCACGTT CAGAGCACCG
  781 TCGAAACGCA GGGAAGCGGT GATGGAGGAC ACGATCTGGG AGATCAGACG GTTCAGGTTG
  841 GTGTAGGTGG GACGCTCGAT GTCCAGGTTA CGACGGCAGA TGTCGTAGAT AGCCTCGTTG
901 TCCACCATGA AAGCGCAGTC GGGAGTGTTC CAGGGTGGTG TGGGTGGTTC AGGATGGG
//
Primer: pP10_F RDB12753_A5Ftc.seq
>12753_12753_A5Ft_pP10_F_D06_12.ab1
    1 TTATAGAATT ATTATCAAAT CATTTGTATA TTAATTAAAA TACTATACTG TAAATTACAT
   61 TTTATTTACA ATCACTCGAC GAAGACTTGA TCACCCGGGA TCTCGAGAAC TCCTAAAAAA
  121 CCGCCACCAT GCGCGAGATC GTGCACATCC AGGCTGGTCA GTGCGGTAAC CAGATCGGTG
  181 CTAAGTTCTG GGAGGTCATC TCCGACGAGC ACGGTATCGA CCCTTCCGGT AACTACGTGG
  241 GTGACTCCGA CCTGCAGCTG GAGCGCATTT CCGTGTACTA CAACGAAGCC TCCTCCCACA
  301 AATACGTGCC TCGCGCTATC CTGGTGGACC TGGAGCCTGG TACTATGGAC TCTGTGCGCT
  361 CCGGTGCTTT CGGTCACCTG TTCCGCCCTG ACAACTTCAT CTTCGGTCAG TCCGGTGCTG
  421 GTAACAACTG GGCTAAGGGT CACTACACCG AGGGTGCTGA GCTCGTCGAC TCCGTGCTGG
  481 ACGTGGTGCG CAAGGAGTGC GAGAACTGCG ACTGCCTGCA GGGTTTCCAG CTGACTCACT
  541 CCCTGGGTGG TGGTACCGGA TCTGGTATGG GTACCCTGCT GATCTCCAAG GTGCGCGAGG
  601 AGTACCCTGA CCGCATCATG AATACCTTCT CCGTCGTGCC TTCCCCTAAG GTGTCCGACA
  661 CCGTGGTGGA GCCTTACAAC GCTACCCTGT CCATCCACCA ACTGGTGGAG AACACCGACG
  721 AGACCTACTG CATCGACAAC GAGGCTCTGT ACGACATCTG CTTCCGCACC CTGAAGCTGG
  781 CTACCCCTAC CTACGGTGAC CTGAACCACC TGGTCTCCGC TACCATGTCC GGTGTGACCA
  841 CCTCCCTGCG CTTCCCTGGT CAGCTGAACG CTGACCTGCG CAAGCTGGCT GTGAACATGG
  901 TGCCTTTCCC TCGCCTGCAC TTCTTCATGC CTGGTTTCGC TCCTCTGACC GCTCGCGGTT
  961 CCCAGCAATA CCGCGCTCTG ACCGTGCCTG AGCTGACCCA GCAGATGTTC GACGCTAAGA
 1021 ACATGATGGC TGCTTGCGAC CCTCGCCACG GGTCGCTACC TGACGTGGCT ACGTGTCCGC
 1081 GGTCGCATGT CCATGATGAG TGGACGAGCA GATGCTGCTA TCAGTTCAGG ACTCTCCTAC
1141 ATCGTTGGAG TGCAATCCTA ACCAAACGGT GGAAAAAG
//
Primer: HSV_TK_pA_R RDB12753_A5Ftd.seq
>12753_12753_A5Ft_HSVTKpA_R_E06_15.ab1
    1 ACGGTCTTCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCATCT CCCGGTACCG
   61 CATGCTATGC ATCAGCTGCT AGCTTACTTG TCATCGTCAT CTTTGTAGTC ACCACCGGAA
  121 CCACCCTTAG GACCCTGAGC CTCGGACTCT TCCTCATCGT CCTCGTACAT CTCACCCTCT
  181 TCTTCAGCGG TAGCGTCCTG GTACTGCTGG TACTCGGACA CCAGGTCGTT CATGTTGGAC
  241 TCAGCCTCGG TGAACTCCAT CTCGTCCATA CCCTCACCGG TGTACCAGTG CAGGAAAGCC
  301 TTGCGGCGGA ACATAGCGGT GAACTGCTCG GAGATGCGCT TGAACAGCTC CTGGATAGCG
  361 GTGGAGTTAC CGATGAAGGT GGAGGACATC TTCAGACCGC GAGGAGGGAT GTCGCACACA
  421 GCCACCTTCA CGTTGTTAGG GATCCACTCC ACGAAGTAGG AGGAGTTCTT GGACTGGATA
  481 GCCAGCATCT GCTCGTCCAC CTCCTTCATG GACATGCGAC CGCGGAACAC GGTAGCCACG
  541 GTCAGGTAGC GACCGTGGCG AGGGTCGCAA GCAGCCATCA TGTTCTTAGC GTCGAACATC
  601 TGCTGGGTCA GCTCAGGCAC GGTCAGAGCG CGGTATTGCT GGGAACCGCG AGCGGTCAGA
  661 GGAGCGAAAC CAGGCATGAA GAAGTGCAGG CGAGGGAAAG GCACCATGTT CACAGCCAGC
  721 TTGCGCAGGT CAGCGTTCAG CTGACCAGGG AAGCGCAGGG AGGTGGTCAC ACCGGACATG
  781 GTAGCGGAGA CCAGGTGGTT CAGGTCACCG TAGGTAGGGG TAGCCAGCTT CAGGGTGCGG
  841 AAGCAGATGT CGTACAGAGC CTCGTTGTCG ATGCAGTAGG TCTCGTCGGT GTTCTCCACC
  901 AGTTGGTGGA TGGACAGGGT AGCGTTGTAA AGCTCCACCA CGGTGTCGGA CACCTTATGG
  961 GAAGGGCACG ACGGAGAAGG TATTCATGAT GCGGTCAGGG TACTCCTCGC GCACCTTGGA
 1021 GATCAGCAGG TACCCATACC AGATCCGGTA CCACCAACCC AGGGAGTGAG TCAGCTGGAA
 1081 AACCCCTGCA GCAGTCGCAG TCTCGCCACT CCTTGCGCAC ACGTTCAGCA CGAGTTCGAA
1141 CGAGCTCAGC ACCCCTCTCG GGTGTTAGTG AACCCCTTAT AGTCCCCAAG T
//
Primer: pBACe3.6_F RDB12753_A5Fte.seq
>12753_12753_A5Ft_pBACe3.6_F_F06_18.ab1
    1 TGTAGCCTGC GTAGCGGGTG TGGGCGGACA ATAAAGTCTT AAACTGAACA AAATAGATCT
   61 AAACTATGAC AATAAAGTCT TAAACTAGAC AGAATAGTTG TAAACTGAAA TCAGTCCAGT
  121 TATGCTGTGA AAAAGCATAC TGGACTTTTG TTATGGCTAA AGCAAACTCT TCATTTTCTG
  181 AAGTGCAAAT TGCCCGTCGT ATTAAAGAGG GGCGTGGCCA AGGGCATGGT AAAGACTATA
  241 TTCGCGGCGT TGTGACAATT TACCGAACAA CTCCGCGGCC GGGAAGCCGA TCTCGGCTTG
  301 AACGAATTGT TAGGTGGCGG TACTTGGGTC GATATCAAAG TGCATCACTT CTTCCCGTAT
  361 GCCCAACTTT GTATAGAGAG CCACTGCGGG ATCGTCACCG TAATCTGCTT GCACGTAGAT
  421 CACATAAGCA CCAAGCGCGT TGGCCTCATG CTTGAGGAGA TTGATGAGCG CGGTGGCAAT
  481 GCCCTGCCTC CGGTGCTCGC CGGAGACTGC GAGATCATAG ATATAGATCT CACTACGCGG
  541 CTGCTCAAAC TTGGGCAGAA CGTAAGCCGC GAGAGCGCCA ACAACCGCTT CTTGGTCGAA
  601 GGCAGCAAGC GCGATGAATG TCTTACTACG GAGCAAGTTC CCGAGGTAAT CGGAGTCCGG
  661 CTGATGTTGG GAGTAGGTGG CTACGTCTCC GAACTCACGA CCGAAAAGAT CAAGAGCAGC
  721 CCGCATGGAT TTGACTTGGT CAGGGCCGAG CCTACATGTG CGAATGATGC CCATACTTGA
  781 GCCACCTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTGCGTAA
  841 CATCGTTGCT GCTCCATAAC ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG
  901 ATGCCCGAGG CATAGACTGT ACAAAAAAAC AGTCATAACA AGCCATGAAA ACCGCCACTG
  961 CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG CGCATACGCT
 1021 ACTTGCATTA CAGTTTACGA CCGAACAGCT TATGTCACTG GGATCGTGCT CATCGTTTCA
 1081 CGGTGTGCGT CACCGCCAGT GGCAGCAGCG AGTCGAGGCA TTCCTGTCGT GGTCTGCGAC
1141 CGAGCGGCCA AGGATTCGGT CTTCCAACGG
//
Primer: pGCAP_R RDB12753_A5Ftf.seq
>12753_12753_A5Ft_pGCAP_R_G06_21.ab1
    1 AGAATCTTAT CATGTCTGGA TCTGATCACT GCTTGAGCCT AGGAGATCCG AACCAGATAA
   61 GTGAAATCTA GTTCCAAACT ATTTTGTCAT TTTTAATTTT CGTATTAGCT TACGACGCTA
  121 CACCCAGTTC CCATCTATTT TGTCACTCTT CCCTAAATAA TCCTTAAAAA CTCCATTTCC
  181 ACCCCTCCCA GTTCCCAACT ATTTTGTCCG CCCACAGCGG GGCATTTTTC TTCCTGTTAT
  241 GTTTTTAATC AAACATCCTG CCAACTCCAT GTGACAAACC GTCATCTTCG GCTACTTTTT
  301 CTCTGTCACA GAATGAAAAT TTTTCTGTCA TCTCTTCGTT ATTAATGTTT GTAATTGACT
  361 GAATATCAAC GCTTATTTGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT
  421 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
  481 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
  541 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
  601 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
  661 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
  721 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
  781 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
  841 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
  901 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT
  961 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTTATTCC
 1021 CTTTTTTGCG GCATTTTGCC TTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAGTAAA
 1081 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTACATCGA CTGGATCTCA CAGCGTAGGA
1141 TTCATGAAGT TTTCGCTCTC GAGAACGGTT TTTCCAATGA TGGAGAGCAC AC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646.

2019.02.26

GNP_filter3_RDBDEP_html_190131.pl