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pNanog-luc

Oct-Sox enhancer carrying reporter derived from Nanog promoter.

Catalog number RDB12116
Resource name pNanog-luc
Clone info. Oct-Sox enhancer derived from Nanog promoter.
Comment PCR fragment of the Nanog promoter region corresponding to nucleotide -332 to +50 was inserted into pGL3-Basic (Masui, S., Nat. Cell Biol., 9 (6), 625-635, 2007).
Vector backbone pGL3-Basic (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nanog genomic DNA
Depositor|Developer Niwa, Hitoshi |
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External Database
Mouse Nanog

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007).
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB12116 pNanog-luc Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB12116z.seq
          Remarks, protocol and/or map (pdf) RDB12116p.pdf

          Original reference

          original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Reguration of genes in ES cells (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

          2022.05.17

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