Resource data sheet
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pNanog-luc

Oct-Sox enhancer carrying reporter derived from Nanog promoter.

Catalog number RDB12116
Resource name pNanog-luc
Clone info. Oct-Sox enhancer derived from Nanog promoter.
PCR fragment of the Nanog promoter region corresponding to nucleotide -332 to +50 was inserted into pGL3-Basic (Masui, S., Nat. Cell Biol., 9 (6), 625-635, 2007).
Vector backbone pGL3-Basic (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nanog genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Nanog (NCBI Gene 71950) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB12116 pNanog-luc Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12116z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB12116p.pdf

Original reference

original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Reguration of genes in ES cells (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

2021.03.25

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