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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pNanog-luc (#RDB12116)

Oct-Sox enhancer carrying reporter derived from Nanog promoter.

Clone info. Oct-Sox enhancer derived from Nanog promoter.
Comment PCR fragment of the Nanog promoter region corresponding to nucleotide -332 to +50 was inserted into pGL3-Basic (Masui, S., Nat. Cell Biol., 9 (6), 625-635, 2007).
Vector backbone pGL3-Basic (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nanog genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB12116z.seq
Remarks, protocol and/or map (pdf) RDB12116p.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

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RDB12116 pNanog-luc DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pNanog-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12116).

Reference section:

Masui, S., Nakatake, Y., Toyooka, Y., Shimosato, D., Yagi, R., Takahashi, K., Okochi, H., Okuda, A., Matoba, R., Sharov, A.A., Ko, M.S., Niwa, H., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12116_A3E9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: PGV_CCw (Pr0129)
Region: insert,pause site,pA signal,f1 ori
Sequence file: RDB12116_A3E9a.seq check
>RDB12116_2_CCW_E07_13.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNCAGCTN NNTTNNATCG CAGATCTCGA GCGCAGCCTT
   61 CCCACAGAAA GAGCAAGACA CCAACCAAAT CAGCCTATCT GAAGGCCAAC GGCTCAAGGC
  121 GATAGATTTA AAGGGTAGGT CCTGCTCCTC CCCCCTCAAG CCTCCTACCC TACCCACCCC
  181 CTATTCTCCC AGGCACCCAG GCGGCACCCA CGCCCCACCA GCCCTGTGAA TTCACAGTTA
  241 ATCCCACCTG CAGGGTCCAC CATGGACATT GTAATGCAAA AGAAGCTGTA AGGTGACCCA
  301 GACTGGGAGG GAGGGAAAGC TTAGGGGGCA TCCTCTGATC TAAAGACATC CCCCCCCCCC
  361 AAAAAGAGGC TTTACCTCAT TTCACTTTAT TCATCTTTTA ACCACGGCTG CACCTCCAGA
  421 CCCTGGCGAT ACGCGTAAGA GCTCGGTACC TATCGATAGA GAAATGTTCT GGCACCTGCA
  481 CTTGCACTGG GGACAGCCTA TTTTGCTAGT TTGTTTTGTT TCGTTTTGTT TTGATGGAGA
  541 GCGTATGTTA GTACTATCGA TTCACACAAA AAACCAACAC ACAGATGTAA TGAAAATAAA
  601 GATATTTTAT TGCGGCCGCT CCAAGTACCT CCCGTACCTT AATATTACTT ACTTATCATG
  661 GTAGCTTGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
  721 GCAGCCTGAA TGGCGAATGG CAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA
  781 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA ANTCGGCAAA ATCCCTTATA
  841 ANTCNAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC NGTTTGGAAC NAGAGTCNNC
  901 TATTAAANAA CGTGGANTCC NNCGTCNAAN GGCGAAAAAC CGTCNATCNN NNNANGNNCN
  961 NCNACGNGNA NNATCNCCNT NANCNNGNTT TTTGGGGNCG ANGNNNNGNA ANGNNNTNNN
1021 NNGNNNNNNN NNN
//
Primer: pGCAP_R (Pr0074)
Region: Luc
Sequence file: RDB12116_A3E9b.seq check
>12116_12116_B3AhF_2_pGCAP_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNGCCGCCC CGACTCTAGA ANTACACGGC GATCTTTCCG CCCTTCTTGG
   61 CCTTTATGAG GATCTCTCTG ATTTTTCTTG CGTCGAGTTT TCCGGTAAGA CCTTTCGGTA
  121 CTTCGTCCAC AAACACAACT CCTCCGCGCA ACTTTTTCGC GGTTGTTACT TGACTGGCGA
  181 CGTAATCCAC GATCTCTTTT TCCGTCATCG TCTTTCCGTG CTCCAAAACA ACAACGGCGG
  241 CGGGAAGTTC ACCGGCGTCA TCGTCGGGAA GACCTGCGAC ACCTGCGTCG AAGATGTTGG
  301 GGTGTTGGAG CAAGATGGAT TCCAATTCAG CGGGAGCCAC CTGATAGCCT TTGTACTTAA
  361 TCAGAGACTT CAGGCGGTCA ACGATGAAGA AGTGTTCGTC TTCGTCCCAG TAAGCTATGT
  421 CTCCAGAATG TAGCCATCCA TCCTTGTCAA TCAAGGCGTT GGTCGCTTCC GGATTGTTTA
  481 CATAACCGGA CATAATCATA GGACCTCTCA CACACAGTTC GCCTCTTTGA TTAACGCCCA
  541 GCGTTTTCCC GGTATCCAGA TCCACAACCT TCGCTTCAAA AAATGGAACA ACTTTACCGA
  601 CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT AGCTGATGTA GTCTCAGTGA
  661 GCCCATATCC TTGCCTGATA CCTGGCAGAT GGAACCTCTT GGCAACCGCT TCCCCGACTT
  721 CCTTAGAGAG GGGAGCGCCA CCANAAGCAA TTTCGTGTAA ATTANATAAA TCGTATTTGT
  781 NNANTCAGAN TGCTTTTGGN GAANAANGAG AANAGGGTTG GCACCNGCAG CGCNCTTTNA
  841 ATCTTGNNAT CCTGAANNNT CCTCANAAAC AGCTCTTCNT NNAAATCNAT NNNNTTNANG
  901 ANNANNCGAN NNCCACANNN TCAAATATCC NANNGNANTA AANNTTNNNA ANNNNTGNNN
  961 NNNNNGNAAN ANNNNNNNAA ATCNNNNNNN CNNGANNNNN TTTGNNTGCN ANAANNNNNN
1021 TNNNNGNNNN GNNANNANNN NNNNNNANNN NNNTNNNNNA NGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932. [link to RRC of NBRP]