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pNanog-luc

Oct-Sox enhancer carrying reporter derived from Nanog promoter.

Catalog number RDB12116
Resource name pNanog-luc
Clone info. Oct-Sox enhancer derived from Nanog promoter.
Comment PCR fragment of the Nanog promoter region corresponding to nucleotide -332 to +50 was inserted into pGL3-Basic (Masui, S., Nat. Cell Biol., 9 (6), 625-635, 2007).
Vector backbone pGL3-Basic (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nanog genomic DNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank
External Database
Mouse Nanog
          Reference sequence
            
           
          Sequence RDB12116z.seq
          Remarks, protocol and/or map (pdf) RDB12116p.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007).
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masui, S., Nat. Cell Biol. 9 (6): 625-635, 2007)。
          付加的提供条件：
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12116 pNanog-luc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pNanog-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12116).

          Reference section:

          Masui, S., Nakatake, Y., Toyooka, Y., Shimosato, D., Yagi, R., Takahashi, K., Okochi, H., Okuda, A., Matoba, R., Sharov, A.A., Ko, M.S., Niwa, H., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Reguration of genes in ES cells (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12116_A3E9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: PGV_CCw (Pr0129)
          Region: insert,pause site,pA signal,f1 ori
          Sequence file: RDB12116_A3E9a.seq check
          >RDB12116_2_CCW_E07_13.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNCAGCTN NNTTNNATCG CAGATCTCGA GCGCAGCCTT
             61 CCCACAGAAA GAGCAAGACA CCAACCAAAT CAGCCTATCT GAAGGCCAAC GGCTCAAGGC
            121 GATAGATTTA AAGGGTAGGT CCTGCTCCTC CCCCCTCAAG CCTCCTACCC TACCCACCCC
            181 CTATTCTCCC AGGCACCCAG GCGGCACCCA CGCCCCACCA GCCCTGTGAA TTCACAGTTA
            241 ATCCCACCTG CAGGGTCCAC CATGGACATT GTAATGCAAA AGAAGCTGTA AGGTGACCCA
            301 GACTGGGAGG GAGGGAAAGC TTAGGGGGCA TCCTCTGATC TAAAGACATC CCCCCCCCCC
            361 AAAAAGAGGC TTTACCTCAT TTCACTTTAT TCATCTTTTA ACCACGGCTG CACCTCCAGA
            421 CCCTGGCGAT ACGCGTAAGA GCTCGGTACC TATCGATAGA GAAATGTTCT GGCACCTGCA
            481 CTTGCACTGG GGACAGCCTA TTTTGCTAGT TTGTTTTGTT TCGTTTTGTT TTGATGGAGA
            541 GCGTATGTTA GTACTATCGA TTCACACAAA AAACCAACAC ACAGATGTAA TGAAAATAAA
            601 GATATTTTAT TGCGGCCGCT CCAAGTACCT CCCGTACCTT AATATTACTT ACTTATCATG
            661 GTAGCTTGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
            721 GCAGCCTGAA TGGCGAATGG CAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA
            781 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA ANTCGGCAAA ATCCCTTATA
            841 ANTCNAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC NGTTTGGAAC NAGAGTCNNC
            901 TATTAAANAA CGTGGANTCC NNCGTCNAAN GGCGAAAAAC CGTCNATCNN NNNANGNNCN
            961 NCNACGNGNA NNATCNCCNT NANCNNGNTT TTTGGGGNCG ANGNNNNGNA ANGNNNTNNN
          1021 NNGNNNNNNN NNN
          //
          Primer: pGCAP_R (Pr0074)
          Region: Luc
          Sequence file: RDB12116_A3E9b.seq check
          >12116_12116_B3AhF_2_pGCAP_R_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNGCCGCCC CGACTCTAGA ANTACACGGC GATCTTTCCG CCCTTCTTGG
             61 CCTTTATGAG GATCTCTCTG ATTTTTCTTG CGTCGAGTTT TCCGGTAAGA CCTTTCGGTA
            121 CTTCGTCCAC AAACACAACT CCTCCGCGCA ACTTTTTCGC GGTTGTTACT TGACTGGCGA
            181 CGTAATCCAC GATCTCTTTT TCCGTCATCG TCTTTCCGTG CTCCAAAACA ACAACGGCGG
            241 CGGGAAGTTC ACCGGCGTCA TCGTCGGGAA GACCTGCGAC ACCTGCGTCG AAGATGTTGG
            301 GGTGTTGGAG CAAGATGGAT TCCAATTCAG CGGGAGCCAC CTGATAGCCT TTGTACTTAA
            361 TCAGAGACTT CAGGCGGTCA ACGATGAAGA AGTGTTCGTC TTCGTCCCAG TAAGCTATGT
            421 CTCCAGAATG TAGCCATCCA TCCTTGTCAA TCAAGGCGTT GGTCGCTTCC GGATTGTTTA
            481 CATAACCGGA CATAATCATA GGACCTCTCA CACACAGTTC GCCTCTTTGA TTAACGCCCA
            541 GCGTTTTCCC GGTATCCAGA TCCACAACCT TCGCTTCAAA AAATGGAACA ACTTTACCGA
            601 CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT AGCTGATGTA GTCTCAGTGA
            661 GCCCATATCC TTGCCTGATA CCTGGCAGAT GGAACCTCTT GGCAACCGCT TCCCCGACTT
            721 CCTTAGAGAG GGGAGCGCCA CCANAAGCAA TTTCGTGTAA ATTANATAAA TCGTATTTGT
            781 NNANTCAGAN TGCTTTTGGN GAANAANGAG AANAGGGTTG GCACCNGCAG CGCNCTTTNA
            841 ATCTTGNNAT CCTGAANNNT CCTCANAAAC AGCTCTTCNT NNAAATCNAT NNNNTTNANG
            901 ANNANNCGAN NNCCACANNN TCAAATATCC NANNGNANTA AANNTTNNNA ANNNNTGNNN
            961 NNNNNGNAAN ANNNNNNNAA ATCNNNNNNN CNNGANNNNN TTTGNNTGCN ANAANNNNNN
          1021 TNNNNGNNNN GNNANNANNN NNNNNNANNN NNNTNNNNNA NGNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932.

          2023.05.01

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