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pcDNA3.1-mycHis-hVASH (#RDB08518)

Expression construct of human VASH (KIAA1036) cDNA

Alternative name human vasohibin-１ (KIAA1036)
Clone info. Expression construct of human VASH (KIAA1036) cDNA. cDNA fragment 913nt to 2061nt of NM_014909.4 is cloned. Myc epitope tag sequence and 6×His tag are not translated because of the stop codon of hVASH in this clone.
Comment Known difference : C to T substitution at 1314nt of NM_014909.4 (Tyr, synonym).
Vector backbone pcDNA3.1/myc-His A (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r
Gene/insert name Human vasohibin-１ cDNA
Depositor|Developer Sato, Yasufumi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Watanabe, K. et al., J. Clin. Invest. 114, 898-907, 2004).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Watanabe, K. et al., J. Clin. Invest. 114, 898-907, 2004)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08518 pcDNA3.1-mycHis-hVASH DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-mycHis-hVASH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08518).

Reference section:

Watanabe, K., Hasegawa, Y., Yamashita, H., Shimizu, K., Ding, Y., Abe, M., Ohta, H., Imagawa, K., Hojo, K., Maki, H., Sonoda, H., Sato, Y., Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis. J. Clin. Invest. 114, 898-907, (2004). PMID 15467828. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08518_A8Atp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB08518_A8Ata.seq check
>08518_08518_A8At_1_CMV-Forward_G01_19_ABI24.ab1
    1 CTTTTCAACA TCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT GGTACCGAGC TCGGATCCAC
  181 TAGTCCAGTG TGGTGGAATT CCCCTCGAAG ATTTAGGGAT GCCAGGGGGG AAGAAGGTGG
  241 CTGGGGGTGG CAGCAGCGGT GCCACTCCAA CGTCCGCTGC GGCCACCGCC CCCTCTGGGG
  301 TCAGGCGTTT GGAGACCAGC GAAGGAACCT CAGCCCAGAG AGATGAGGAG CCAGAAGAGG
  361 AAGGGGAAGA GGACCTGCGA GACGGAGGCG TCCCCTTCTT TGTCAACCGG GGTGGGCTAC
  421 CTGTGGATGA GGCCACCTGG GAAAGGATGT GGAAACACGT GGCCAAGATC CACCCCGATG
  481 GAGAGAAGGT GGCGCAACGG ATCCGTGGGG CCACAGACCT GCCCAAGATC CCCATACCGA
  541 GTGTGCCTAC GTTCCAGCCG TCTACACCTG TCCCTGAGCG CCTGGAAGCT GTGCAGCGCT
  601 ATATCAGAGA GCTGCAGTAC AATCACACAG GGACACAGTT CTTTGAAATT AAGAAGAGCA
  661 GACCTCTGAC AGGGCTGATG GACCTGGCCA AGGAAATGAC CAAAGAGGCC CTGCCAATCA
  721 AATGCCTGGA AGCCGTGATC CTGGGAATTT ACCTCACCAA CAGCATGCCC ACCCTGGAGC
  781 GCTTCCCCAT CAGCTTCAAG ACCTACTTCT CAGGGAACTA CTTCCGCCAC ATCGTGCTGG
  841 GGGGTGAACT TCGCGGGCCG CTACGGTGCG CTGGGGCATG AGTCGGCGCG AGGACCTGAT
  901 GTACAAGCCG CCCGCCTTCC GCACGCTCAG CGAGCTCGTG CTGGGACTTC GAGGCCGCCC
  961 TACGGCCGCT GCTGGCACGT GCTCAGAAGG TGAAGCTGGG TCAGAGCGTG TCACACGACC
 1021 CGCACAGCGT TGAGCCAGAT CGAGTGAGCA ACTTCGGATG CCTGACGGAT GAAGCCGGTC
1081 T
//
Primer: BGH_rev2
Sequence file: RDB08518_A8Atb.seq check
>08518_08518_A8At_1_BGH_rev2_H01_22_ABI24.ab1
    1 CTTGGGGGTT AGGGAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACT CAATGGTGAT GGTGATGATG ACCGGTATGC
  121 ATATTCAGAT CCTCTTCTGA GATGAGTTTT TGTTCGAATG GGTGACCTCG AGTGGGTGGG
  181 GCCTGGGGTG CTGGCATCCG CCTCAGACCC GGATCTGGTA CCCGTTAAGG TCTGGCATGG
  241 CTTTGGGCTC GGAAGTCTTC TTGTCACCCG AGGGCCGTCT TTCACTGCGG CTGTTCCTGC
  301 GGTGGGGGCT GGACTGGGCC CGCTGCGGGG AAGAAACATC CTTCTTCCGG TCCTTGGTGG
  361 GAGAGGGAGG GCCCGTCCCT TTGCCAATCT TGAGCCGCAT GTCGCGGGCG TGGCGCTCCA
  421 GCTCCTTGCG GAAGTCATCG CGGCCCAGGC GCTCCACGTC CAGCACCGAG TGCTTCCACT
  481 CGATCTGCTC CACGCTGTGC GGGTCGTGTG ACACGCTCTG GCCCAGCTTC ACCTTCTTGA
  541 GCACGTGCCA GCAGCGGCCG TAGGCGGCCT CGAAGTCCAG CACGAGCTCG CTGAGCGTGC
  601 GGAAGGCGGG CGGCTTGTAC ATCAGGTCCT CGCGCCGACT CATGCCCAGC GCACCGTAGC
  661 GGCCCGCGAA GTTCACCCCC AGCACGATGT GGCGGAAGTA GTTCCCTGAG AAGTAGGTCT
  721 TGAAGCTGAT GGGGAAGCGC TCCAGGGTGG GCATGCTGTT GGTGAGGTAA ATTCCCAGGA
  781 TCACGGCTTC CAGGCATTTG ATTGGCAGGG CCCTCTTTGG TCATTTCCTT GGCCAGGTCC
  841 ATCAGCCCTG TCAGAGGTCT GCTCTTCTTA ATTTCAAAGA ACTGTGTCCC TGTGTGATTG
  901 TACTGCAGCT CTCTGATATA GCGCTGCACA GCTTCCCAGG CGCTCAGGGA CAGTGTAGAC
  961 GGCTGGGAAC GTAGGCACAC TCGGTATGGG ATCTGGCAGG TCTGTGCCCC ACGGATCCGT
 1021 TGCGCAACCT CTCTCAATCC GGGGTGAATC CTGGCCACGC GATTTCGACA TTCCCTTTTC
//
Primer: SV40pro_F
Sequence file: RDB08518_A8Atc.seq check
>08518_08518_A8At_1_SV40pro_F_A02_02_ABI24.ab1
    1 ACCTGGGTCC TGAGCATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GCGAAATGAC CGACCCAGCG
  961 ACGCCCAAAC CCTGCCATCA CGAGATTTCG ATTTCCCACC GCCCGCCCTT CTATGAAAGG
 1021 TGGGCTTCGG AATCGTTTTC AGGAACGCGG CTGGAATGAT CCTTCAGCGC GGGGATCTCA
1081 TGCCTGGGAG GTTTCGTTCG GC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, K., Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis. J. Clin. Invest. 114, 898-907, (2004). PMID 15467828. [link to RRC of NBRP]