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pEGFP-MBD-nls (#RDB08344)

Methylated DNA probe. Expression clone of methylated DNA binding domain (MBD) of human MBD1.

Alternative name pCAGGS-EGFP-MBD-nls
Clone info. Methylated DNA probe. Expression clone of methylated DNA binding domain (MBD) of human methyl CpG-binding domain protein 1 (MBD1).
Comment Mouse ES cell line EGFP-MBD-nls (cat# AES0134) is available from the Cell Bank, RIKEN BRC.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Human methyl CpG-binding domain protein 1 (MBD1) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Abe, Kuniya |
 
Remarks, protocol and/or map (pdf) RDB08344.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Cells, 12, 447-460 (2007).).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes Cells, 12, 447-460 (2007).)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08344 pEGFP-MBD-nls DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-MBD-nls was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08344).

Reference section:

Kobayakawa, S., Miike, K., Nakao, M., Abe, K., Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells, 12, 447-460 (2007). PMID 17397393. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08344_A4Ed.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB08344_A4Eda.seq check
>08344_08344_A4Ed_pAxCA_F1_A05_02.ab1
    1 CCTAGACACC CGGGGGGGCC GCTACAGGGT GGATGCCTTC TTCTTTTTCC TACAGCTCCT
   61 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AGCGCTACCG
  121 GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
  181 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
  241 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
  301 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC
  361 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
  421 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
  481 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
  541 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG
  601 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG
  661 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
  721 GACAACCACT ACCTGAGCAC CCAGTCCGCC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
  781 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT
  841 GTACAAGTCC GGGACTCAGA TCTCGAGGCT CAAGGCTTTC GAAATTCTGC AGTCGACGGT
  901 ACCCTCTAGA ATGGGCTGAG GACTGGCTGG GACTGCCCGG GCCCTGGGGC CCTGGGCTGG
  961 AAGCGCCCGC GAAGTCTTTC GCCAAGTTCA GGGGGCCACC TGGTGGGACG CTTCAAGACC
 1021 ACCTTATTTA CCAGAGGCTC CTACAAGGAG ACAAGGGATT CCGAAAGCAA AAGTTTGGAA
 1081 GCTTGAACTT CGAATACCTT GGGGCCCCTT GCCGGTGTGA ATCTCTCACC CCTTCCTTTC
 1141 CGAACTTTCA AACCAGGCAA TCTTGGGTGC CTATCCCAGC CCCCTCCAAG GGCCCCAATT
1201 TCTCCGTCGT GAC
//
Primer: EGFP-C
Sequence file: RDB08344_A4Edb.seq check
>08344_08344_A4Ed_EGFP-C_A03_03.ab1
    1 CAGTAATCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
   61 GAATTCTGCA GTCGACGGTA CCTCTAGAAT GGCTGAGGAC TGGCTGGACT GCCCGGCCCT
  121 GGGCCCTGGC TGGAAGCGCC GCGAAGTCTT TCGCAAGTCA GGGGCCACCT GTGGACGCTC
  181 AGACACCTAT TACCAGAGCC CCACAGGAGA CAGGATCCGA AGCAAAGTTG AGCTGACTCG
  241 ATACCTGGGC CCTGCGTGTG ATCTCACCCT CTTCGACTTC AAACAAGGCA TCTTGTGCTA
  301 TCCAGCCCCC AAGGCCCATC CCGTGGCGGT TGCCAGCAAG AAGCGAAAGA AGCCTTCAAG
  361 GCCAGCCAAG ACTCGGAAAC GTCAGGTTGG ACCCCAGAGT GGTGAGGTCA GGAAGGAGGC
  421 CCCGTCTAGA GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG
  481 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
  541 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG
  601 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC
  661 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC GAATTCACTC CTCAGGTGCA
  721 GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG
  781 AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC
  841 TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTTGTGTC
  901 TCTCACTCGG AAGGACATAT GGGGAGGGCA AATCATTTAA ACATCAGAAT GAGTATTTGG
  961 TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAGGTT GGGCTATAAG
 1021 AGGTCATCAG TATATGGAAC AGCCCCCTGC TGTCCATTCC CTAATCCATA GAAAGCCTTG
1081 AACTGAGTTA GATTTTTTTA TAGTTGCTTT TGTGCTAAAT TTTTTCGTTT GAAC
//
Primer: pCA-bgter
Sequence file: RDB08344_A4Edc.seq check
>08344_08344_A4Ed_PCA-bgter_B03_06.ab1
    1 AGCCCAGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
  121 CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG
  181 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA
  241 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA
  301 GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC
  361 TAGATCCGGT GGATCCCGGG CCCGCGGTAC CTCTAGACGG GGCCTCCTTC CTGACCTCAC
  421 CACTCTGGGG TCCAACCTGA CGTTTCCGAG TCTTGGCTGG CCTTGAAGGC TTCTTTCGCT
  481 TCTTGCTGGC AACCGCCACG GGATGGGCCT TGGGGGCTGG ATAGCACAAG ATGCCTTGTT
  541 TGAAGTCGAA GAGGGTGAGA TCACACGCAG GGCCCAGGTA TCGAGTCAGC TCAACTTTGC
  601 TTCGGATCCT GTCTCCTGTG GGGCTCTGGT AATAGGTGTC TGAGCGTCCA CAGGTGGCCC
  661 CTGACTTGCG AAAGACTTCG CGGCGCTTCC AGCCAGGGCC CAGGGCCGGG CAGTCCAGCC
  721 AGTCCTCAGC CATTCTAGAG GTACCGTCGA CTGCAGAATT CGAAGCTTGA GCTCGAGATC
  781 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
  841 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC
  901 TCAGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCC GATGGGGGGT GTTCTGCTGG
  961 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CCTCGATGTT GTGGCCGGAT CTTGAAGTTC
 1021 ACCTTGATGC CGTTCTTCTG CTTGTTCGGC CATGATATAG ACGTTTGTGG CCTTGTTGTA
 1081 GGTTGTACTC CAAGCTTGTG CCCCCAGGAA TGTTGGCCGT CTCTTGAGTC GAATGGCCCT
1141 TCAGCCTCGA TGGCGGGTTT TCACCAGGGG GTGGGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kobayakawa, S., Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells, 12, 447-460 (2007). PMID 17397393. [link to RRC of NBRP]
reference Kubiura, M., Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cells. Chromosome Res. 20 (7): 837-848 (2012). PMID 23111490.