Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-MBD-nls

Methylated DNA probe. Expression clone of methylated DNA binding domain (MBD) of human MBD1.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Cells, 12, 447-460 (2007).).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes Cells, 12, 447-460 (2007).)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08344_A4Ed.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08344_A4Eda.seq
>08344_08344_A4Ed_pAxCA_F1_A05_02.ab1     1 CCTAGACACC CGGGGGGGCC GCTACAGGGT GGATGCCTTC TTCTTTTTCC TACAGCTCCT    61 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AGCGCTACCG   121 GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC   181 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT   241 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC   301 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC   361 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC   421 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC   481 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC   541 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG   601 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG   661 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC   721 GACAACCACT ACCTGAGCAC CCAGTCCGCC CCTGAGCAAA GACCCCAACG AGAAGCGCGA   781 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT   841 GTACAAGTCC GGGACTCAGA TCTCGAGGCT CAAGGCTTTC GAAATTCTGC AGTCGACGGT   901 ACCCTCTAGA ATGGGCTGAG GACTGGCTGG GACTGCCCGG GCCCTGGGGC CCTGGGCTGG   961 AAGCGCCCGC GAAGTCTTTC GCCAAGTTCA GGGGGCCACC TGGTGGGACG CTTCAAGACC  1021 ACCTTATTTA CCAGAGGCTC CTACAAGGAG ACAAGGGATT CCGAAAGCAA AAGTTTGGAA  1081 GCTTGAACTT CGAATACCTT GGGGCCCCTT GCCGGTGTGA ATCTCTCACC CCTTCCTTTC  1141 CGAACTTTCA AACCAGGCAA TCTTGGGTGC CTATCCCAGC CCCCTCCAAG GGCCCCAATT 1201 TCTCCGTCGT GAC //
Primer: EGFP-C Sequence file: RDB08344_A4Edb.seq
>08344_08344_A4Ed_EGFP-C_A03_03.ab1     1 CAGTAATCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC    61 GAATTCTGCA GTCGACGGTA CCTCTAGAAT GGCTGAGGAC TGGCTGGACT GCCCGGCCCT   121 GGGCCCTGGC TGGAAGCGCC GCGAAGTCTT TCGCAAGTCA GGGGCCACCT GTGGACGCTC   181 AGACACCTAT TACCAGAGCC CCACAGGAGA CAGGATCCGA AGCAAAGTTG AGCTGACTCG   241 ATACCTGGGC CCTGCGTGTG ATCTCACCCT CTTCGACTTC AAACAAGGCA TCTTGTGCTA   301 TCCAGCCCCC AAGGCCCATC CCGTGGCGGT TGCCAGCAAG AAGCGAAAGA AGCCTTCAAG   361 GCCAGCCAAG ACTCGGAAAC GTCAGGTTGG ACCCCAGAGT GGTGAGGTCA GGAAGGAGGC   421 CCCGTCTAGA GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG   481 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA   541 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG   601 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC   661 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC GAATTCACTC CTCAGGTGCA   721 GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG   781 AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC   841 TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTTGTGTC   901 TCTCACTCGG AAGGACATAT GGGGAGGGCA AATCATTTAA ACATCAGAAT GAGTATTTGG   961 TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAGGTT GGGCTATAAG  1021 AGGTCATCAG TATATGGAAC AGCCCCCTGC TGTCCATTCC CTAATCCATA GAAAGCCTTG 1081 AACTGAGTTA GATTTTTTTA TAGTTGCTTT TGTGCTAAAT TTTTTCGTTT GAAC //
Primer: pCA-bgter Sequence file: RDB08344_A4Edc.seq
>08344_08344_A4Ed_PCA-bgter_B03_06.ab1     1 AGCCCAGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT TTGTGAGCCA    61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT   121 CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG   181 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA   241 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA   301 GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC   361 TAGATCCGGT GGATCCCGGG CCCGCGGTAC CTCTAGACGG GGCCTCCTTC CTGACCTCAC   421 CACTCTGGGG TCCAACCTGA CGTTTCCGAG TCTTGGCTGG CCTTGAAGGC TTCTTTCGCT   481 TCTTGCTGGC AACCGCCACG GGATGGGCCT TGGGGGCTGG ATAGCACAAG ATGCCTTGTT   541 TGAAGTCGAA GAGGGTGAGA TCACACGCAG GGCCCAGGTA TCGAGTCAGC TCAACTTTGC   601 TTCGGATCCT GTCTCCTGTG GGGCTCTGGT AATAGGTGTC TGAGCGTCCA CAGGTGGCCC   661 CTGACTTGCG AAAGACTTCG CGGCGCTTCC AGCCAGGGCC CAGGGCCGGG CAGTCCAGCC   721 AGTCCTCAGC CATTCTAGAG GTACCGTCGA CTGCAGAATT CGAAGCTTGA GCTCGAGATC   781 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT   841 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC   901 TCAGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCC GATGGGGGGT GTTCTGCTGG   961 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CCTCGATGTT GTGGCCGGAT CTTGAAGTTC  1021 ACCTTGATGC CGTTCTTCTG CTTGTTCGGC CATGATATAG ACGTTTGTGG CCTTGTTGTA  1081 GGTTGTACTC CAAGCTTGTG CCCCCAGGAA TGTTGGCCGT CTCTTGAGTC GAATGGCCCT 1141 TCAGCCTCGA TGGCGGGTTT TCACCAGGGG GTGGGTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl