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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-MBD-nls

Methylated DNA probe. Expression clone of methylated DNA binding domain (MBD) of human MBD1.

Catalog number RDB08344
Resource name pEGFP-MBD-nls
Alternative name pCAGGS-EGFP-MBD-nls
Clone info. Methylated DNA probe. Expression clone of methylated DNA binding domain (MBD) of human methyl CpG-binding domain protein 1 (MBD1).
Comment Mouse ES cell line EGFP-MBD-nls (cat# AES0134) is available from the Cell Bank, RIKEN BRC.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Human methyl CpG-binding domain protein 1 (MBD1) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Abe, Kuniya |
Other clones in our bank

External Database
Human methyl CpG-binding domain protein 1 (MBD1) Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Cells, 12, 447-460 (2007).).
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes Cells, 12, 447-460 (2007).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08344 pEGFP-MBD-nls DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB08344.pdf

          Original reference

          original Kobayakawa, S., Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells, 12, 447-460 (2007). PMID 17397393.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content GFP Resources (English text)
          Featured content Epigenetics clone (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08344_A4Ed.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB08344_A4Eda.seq check
          >08344_08344_A4Ed_pAxCA_F1_A05_02.ab1
              1 CCTAGACACC CGGGGGGGCC GCTACAGGGT GGATGCCTTC TTCTTTTTCC TACAGCTCCT
             61 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AGCGCTACCG
            121 GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
            181 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
            241 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
            301 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC
            361 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
            421 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
            481 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
            541 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG
            601 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG
            661 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
            721 GACAACCACT ACCTGAGCAC CCAGTCCGCC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
            781 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT
            841 GTACAAGTCC GGGACTCAGA TCTCGAGGCT CAAGGCTTTC GAAATTCTGC AGTCGACGGT
            901 ACCCTCTAGA ATGGGCTGAG GACTGGCTGG GACTGCCCGG GCCCTGGGGC CCTGGGCTGG
            961 AAGCGCCCGC GAAGTCTTTC GCCAAGTTCA GGGGGCCACC TGGTGGGACG CTTCAAGACC
           1021 ACCTTATTTA CCAGAGGCTC CTACAAGGAG ACAAGGGATT CCGAAAGCAA AAGTTTGGAA
           1081 GCTTGAACTT CGAATACCTT GGGGCCCCTT GCCGGTGTGA ATCTCTCACC CCTTCCTTTC
           1141 CGAACTTTCA AACCAGGCAA TCTTGGGTGC CTATCCCAGC CCCCTCCAAG GGCCCCAATT
          1201 TCTCCGTCGT GAC
          //
          Primer: EGFP-C
          Sequence file: RDB08344_A4Edb.seq check
          >08344_08344_A4Ed_EGFP-C_A03_03.ab1
              1 CAGTAATCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
             61 GAATTCTGCA GTCGACGGTA CCTCTAGAAT GGCTGAGGAC TGGCTGGACT GCCCGGCCCT
            121 GGGCCCTGGC TGGAAGCGCC GCGAAGTCTT TCGCAAGTCA GGGGCCACCT GTGGACGCTC
            181 AGACACCTAT TACCAGAGCC CCACAGGAGA CAGGATCCGA AGCAAAGTTG AGCTGACTCG
            241 ATACCTGGGC CCTGCGTGTG ATCTCACCCT CTTCGACTTC AAACAAGGCA TCTTGTGCTA
            301 TCCAGCCCCC AAGGCCCATC CCGTGGCGGT TGCCAGCAAG AAGCGAAAGA AGCCTTCAAG
            361 GCCAGCCAAG ACTCGGAAAC GTCAGGTTGG ACCCCAGAGT GGTGAGGTCA GGAAGGAGGC
            421 CCCGTCTAGA GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG
            481 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
            541 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG
            601 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC
            661 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC GAATTCACTC CTCAGGTGCA
            721 GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG
            781 AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC
            841 TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTTGTGTC
            901 TCTCACTCGG AAGGACATAT GGGGAGGGCA AATCATTTAA ACATCAGAAT GAGTATTTGG
            961 TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAGGTT GGGCTATAAG
           1021 AGGTCATCAG TATATGGAAC AGCCCCCTGC TGTCCATTCC CTAATCCATA GAAAGCCTTG
          1081 AACTGAGTTA GATTTTTTTA TAGTTGCTTT TGTGCTAAAT TTTTTCGTTT GAAC
          //
          Primer: pCA-bgter
          Sequence file: RDB08344_A4Edc.seq check
          >08344_08344_A4Ed_PCA-bgter_B03_06.ab1
              1 AGCCCAGAAG TCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT TTGTGAGCCA
             61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
            121 CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG
            181 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA
            241 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA
            301 GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC
            361 TAGATCCGGT GGATCCCGGG CCCGCGGTAC CTCTAGACGG GGCCTCCTTC CTGACCTCAC
            421 CACTCTGGGG TCCAACCTGA CGTTTCCGAG TCTTGGCTGG CCTTGAAGGC TTCTTTCGCT
            481 TCTTGCTGGC AACCGCCACG GGATGGGCCT TGGGGGCTGG ATAGCACAAG ATGCCTTGTT
            541 TGAAGTCGAA GAGGGTGAGA TCACACGCAG GGCCCAGGTA TCGAGTCAGC TCAACTTTGC
            601 TTCGGATCCT GTCTCCTGTG GGGCTCTGGT AATAGGTGTC TGAGCGTCCA CAGGTGGCCC
            661 CTGACTTGCG AAAGACTTCG CGGCGCTTCC AGCCAGGGCC CAGGGCCGGG CAGTCCAGCC
            721 AGTCCTCAGC CATTCTAGAG GTACCGTCGA CTGCAGAATT CGAAGCTTGA GCTCGAGATC
            781 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
            841 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC
            901 TCAGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCC GATGGGGGGT GTTCTGCTGG
            961 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CCTCGATGTT GTGGCCGGAT CTTGAAGTTC
           1021 ACCTTGATGC CGTTCTTCTG CTTGTTCGGC CATGATATAG ACGTTTGTGG CCTTGTTGTA
           1081 GGTTGTACTC CAAGCTTGTG CCCCCAGGAA TGTTGGCCGT CTCTTGAGTC GAATGGCCCT
          1141 TCAGCCTCGA TGGCGGGTTT TCACCAGGGG GTGGGTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kobayakawa, S., Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells, 12, 447-460 (2007). PMID 17397393.
          reference Kubiura, M., Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cells. Chromosome Res. 20 (7): 837-848 (2012). PMID 23111490 .

          2022.05.17

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