Resource data sheet
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pET25b_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

Catalog number RDB08309
Resource name pET25b_Tr-egl2-His
Alternative name pET25b_Tr004-His
Clone info. Biomass collection. the Endoglucanase EG-II (EGLII, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl2 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08309 pET25b_Tr-egl2-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08309z.seq from Depositor
Electronic file PDF RDB08309.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08309_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08309_A5Iia.seq
>08309_08309_A5Ii_T7_20bp_C01_07.ab1
    1 TTCCCGGATC AAATTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT
   61 ATGAAATACC TGCTGCCGAC CGCTGCTGCT GGTCTGCTGC TCCTCGCTGC CCAGCCGGCG
  121 ATGGCCATGG ATATCGGAAT TAATTCGGAT CCGAATTCGA GCTCCGTCGA CCAGCAGACT
  181 GTCTGGGGCC AGTGTGGAGG TATTGGTTGG AGCGGACCTA CGAATTGTGC TCCTGGCTCA
  241 GCTTGTTCGA CCCTCAATCC TTATTATGCG CAATGTATTC CGGGAGCCAC TACTATCACC
  301 ACTTCGACCC GGCCACCATC CGGTCCAACC ACCACCACCA GGGCTACCTC AACAAGCTCA
  361 TCAACTCCAC CCACGAGCTC TGGGGTCCGA TTTGCCGGCG TTAACATCGC GGGTTTTGAC
  421 TTTGGCTGTA CCACAGATGG CACTTGCGTT ACCTCGAAGG TTTATCCTCC GTTGAAGAAC
  481 TTCACCGGCT CAAACAACTA CCCCGATGGC ATCGGCCAGA TGCAGCACTT CGTCAACGAC
  541 GACGGGATGA CTATTTTCCG CTTACCTGTC GGATGGCAGT ACCTCGTCAA CAACAATTTG
  601 GGCGGCAATC TTGATTCCAC GAGCATTTCC AAGTATGATC AGCTTGTTCA GGGGTGCCTG
  661 TCTCTGGGCG CATACTGCAT CGTCGACATC CACAATTATG CTCGATGGAA CGGTGGGATC
  721 ATTGGTCAGG GCGGCCCTAC TAATGCTCAA TTCACGAGCC TTTGGTCGCA GTTGGCATCA
  781 AAGTACGCAT CTCAGTCGAG GGTGTGGTTC GGCATCATGA ATGAGCCCCA CGACGTGAAC
  841 ATCAACACCT GGGCTGCCAC GGTCCAAGAG GTTGTAACCG CAAATCCGCA ACGCTGGTGC
  901 TACGTCGCAA TTCATCTCTT TTGCCTGGAA ATGATTGGCA ATCTGCTGGG GCTTTCATAA
  961 TCCGATGGCA GTGCAGCCCG CCCTGGTCCT CCAAGTTCAC GAACCCGGGA TGGGGTCAAA
 1021 CAACGAATTC TGAATTTTTT GACGTGCACA ATTACCTTGG AACTCAGACC CAACTTCCCG
1081 GTAACTCCAC GTCG
//
Primer: f1ori_R3 RDB08309_A5Iib.seq
>08309_08309_A5Ii_f1ori_R3_D01_10.ab1
    1 GTAACCGGCA ATTCGGGGAA TAGTTCCTCC TTTCAGCAAA AACCCCTCAA GACCCGTTTA
   61 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC
  121 TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GACATCCTCG
  181 GGGTCTTCCG GGGCGAGTTC TGGCTGGCTA GCCCGTTTGA TCTCGAGTGC GGCCGCAAGC
  241 TTGTCGACCT TTCTTGCGAG ACACGAGCTG ACCAAGGATG TGTCCGTCCA TGAGTTACCA
  301 CTGCCAGTCG GTGTTTCCGT CAGGACATAC GTGCTATCAA ATGATCCGGC ACCCCAACCA
  361 ACATAGCCAA GATAGACATC TGAGTTCTGG TTGAGATATT GGATTTGCTG GCACATGTCT
  421 TGTATGCAGG ACTGAACGTT GCCACCACCG GTTTCTGTCA GGATAGCCTG GCGATTGTTC
  481 TGTCGGAGCC AAGTGGCAAG CGGAGAAAAG GCGCCGTCAA TGTTATTTGT AGTACATTCG
  541 GCGTGAGTAC CGGAGTTGTC TGAGTCCAAG TATTTTGTGC ACGTCAAAAA TCAGATTCGT
  601 TGTTGACCCA TCCGGGGTTC GGGACTTGAG ACAGGGGCGG CTGCCCTGCC ATCCGGAAAT
  661 GAAAGCCCCA CAAATTGCCA ATCCATTTCC AGGCAAAAAA AATGAATTTG CGAACTAGAC
  721 CCGCGTTGGC GGATTGGCGG TTACAACCCC CTTGGACCGG GGGAACCCCA GGGGTTAAAG
781 TTTCCCTCTT GGGGGCTTAT TTTCTTGAAG GCGAAACCCC CCCCCTCCAT GGAAAAGCG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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