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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET25b_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

Catalog number RDB08309
Resource name pET25b_Tr-egl2-His
Alternative name pET25b_Tr004-His
Clone info. Biomass collection. the Endoglucanase EG-II (EGLII, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl2 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08309 pET25b_Tr-egl2-His DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08309z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08309.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17893_B0A6p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F
Region: T7 pro, lacO, RBS, pelB signal, insert5'
Sequence file: RDB17893_B0A6a.seq check
>08309_17893_B0A6_1_pET-B_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNGCC GGCNNCGATG CGTCCGGCGT ANAGGNNCGA GATCTCGATC
   61 CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG
  121 AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG AAATACCTGC TGCCGACCGC
  181 TGCTGCTGGT CTGCTGCTCC TCGCTGCCCA GCCGGCGATG GCCATGGATA TCGGAATTAA
  241 TTCGGATCCG AATTCGAGCT CCGTCGACCA GCAGACTGTC TGGGGCCAGT GTGGAGGTAT
  301 TGGTTGGAGC GGACCTACGA ATTGTGCTCC TGGCTCAGCT TGTTCGACCC TCAATCCTTA
  361 TTATGCGCAA TGTATTCCGG GAGCCACTAC TATCACCACT TCGACCCGGC CACCATCCGG
  421 TCCAACCACC ACCACCAGGG CTACCTCAAC AAGCTCATCA ACTCCACCCA CGAGCTCTGG
  481 GGTCCGATTT GCCGGCGTTA ACATCGCGGG TTTTGACTTT GGCTGTACCA CAGATGGCAC
  541 TTGCGTTACC TCGAAGGTTT ATCCTCCGTT GAAGAACTTC ACCGGCTCAA ACAACTACCC
  601 CGATGGCATC GGCCAGATGC AGCACTTCGT CAACGACGAC GGGATGACTA TTTTCCGCTT
  661 ACCTGTCGGA TGGCAGTACC TCGTCAACAA CAATTTGGGC GGCAATCTTG ATTCCACGAG
  721 CATTTCCNAG TATGATCAGC TTGTTCAGGN NTGCCTGTCT CTGGGCGCAT ACTGCATCGT
  781 CGACATCCAC AATTATGCTC GATGGAACGG NGNNATCATT GGTCAGGGCG GCCCTACTAA
  841 TGCTCNATTC ACGANCCTTN GNNCGCANTT GGCATCAAAG TACGCATCTC ANNCNANGGN
  901 GTGGTTCNGN ATCNTGAATG ANCCCNNCGA NNNNNNNNTC NNNNCCNNGG NNNNNNCGNN
961 NNNNNANNNN NNNNNN
//
Primer: f1ori_R3
Region: T7 ter, 6xHis, HSV tag, insert3'
Sequence file: RDB17893_B0A6b.seq check
>08309_17893_B0A6_1_f1ori_R3_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCCTTTNN GCNNAAAACC CCTCNAGANC CGTTTAGAGG
   61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
  121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGACA TCCTCGGGGT
  181 CTTCCGGGGC GAGTTCTGGC TGGCTAGCCC GTTTGATCTC GAGTGCGGCC GCAAGCTTGT
  241 CGACCTTTCT TGCGAGACAC GAGCTGACCA AGGATGTGTC CGTCCATGAG TTACCACTGC
  301 CAGTCGGTGT TTCCGTCAGG ACATACGTGC TATCAAATGA TCCGGCACCC CAACCAACAT
  361 AGCCAAGATA GACATCTGAG TTCTGGTTGA GATATTGGAT TTGCTGGCAC ATGTCTTGTA
  421 TGCAGGACTG AACGTTGCCA CCACCGGTTT CTGTCAGGAT AGCCTGGCGA TTGTTCTGTC
  481 GGAGCCAAGT GGCAAGCGGA GAAAAGGCGC CGTCAATGTT ATTTGTAGTA CATTCGGCGT
  541 GAGTACCGGA GTTGTCTGAG TCCAAGTATT TGTGCACGTC AAAAATCAGA TTCGTTGTTG
  601 ACCCATCCGG GTTCGTGACT TGAGACAGGG CGGCTGCACT GCCATCGGAT ATGAAAGCCC
  661 CAGCAGATTG CCAATCATTT CNAGGCAAAG AGATGAATTG CGACGTAGCA CCAGCGTTGC
  721 GGATTGCGGT TACAACCTCT TGGANCGTGG CAGCCCANGT GTTGATGTTC ACGTCGTNNN
  781 GCTCATTCAT GATGCCGAAC CACACCCTCG ACTGANATGC GTACTTTGAT GNCAACTNCN
  841 ANCAAANGNT CGNGANTTGA NCATTAGTAN GGCCGCCCNN ANCAATGATN CCACCGNTNC
  901 NTCNANCNTA ATNNNNNNTG TCGANNANGC ANTATGCNNC CANNNNNAGG NNNNNNNNNA
961 NNNANNNNAN NNNNNNNNN
//
Primer: lacIpro_F2
Region: lacI gene
Sequence file: RDB17893_B0A6c.seq check
>08309_17893_B0A6_1_laclpro_F2_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNG GNNNCGCATA AGGGNNANCG TCGAGNTCCC GGNNACCATC
   61 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG
  121 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT
  181 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA
  241 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG
  301 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG
  361 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG
  421 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA
  481 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA
  541 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC
  601 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG
  661 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT
  721 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA
  781 NGCGANTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGANGGCATC
  841 GTTCCNNCTG CNATGCTGGT TGCCNACGAT CAGATGGCGC NNGGCGCANT GCGCGCCATT
  901 ANNNANNNCG GGNNGCNCGT TGGNNCNGNN NATNNCNNNA NNNGGNNACN ANNANNCCGN
961 ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl