Sequencing and PCR primers for primer information.
Expression vector of Endoglucanase Cel74a of Trichoderma reesei
| Catalog number | RDB08298 |
|---|---|
| Resource name | pET21c_Tr-cel74a-His |
| Alternative name | pET21c_Tr021-His |
| Clone info. | Biomass collection. the Endoglucanase Cel74a (Endo-1,4-beta-glucanase) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c. |
| Vector backbone | pET-21c (Plasmid) |
| Size of vector backbone | 5441 |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei cel74a cDNA |
| Depositor | DNA Bank, | Kishikawa, Shotaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08298 | pET21c_Tr-cel74a-His | DNA solution |
Electronic file
| Electronic file | Sequence RDB08298z.seq from Depositor |
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| Electronic file | PDF RDB08298.pdf from Depositor |
Featured content
| Featured content | Biomass Collection (English text) |
|---|---|
| Featured content | Biomass Collection (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08298_A6G4p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB08298_A6G4a.seq |
|---|---|
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>08298_08298_A6G4_T7_20bp_D07_10.ab1 1 TGGTCGTTCC CATCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCTA 61 GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG ACCGCCTTTT 121 CATGGAAGAA CGTCAAGCTC GGCGGCGGCG GCGGCTTCGT CCCCGGCATC ATCTTCCATC 181 CCAAGACAAA AGGCGTAGCA TATGCACGAA CAGATATTGG CGGGCTGTAC CGCCTCAACG 241 CCGACGACTC ATGGACCGCC GTCACGGATG GGATTGCTGA TAATGCCGGC TGGCACAACT 301 GGGGCATCGA CGCTGTTGCG CTTGATCCGC AGGACGATCA AAAGGTGTAT GCCGCAGTCG 361 GCATGTATAC GAACAGCTGG GATCCGAGTA ATGGAGCCAT CATTCGCTCG TCAGACCGCG 421 GCGCAACGTG GTCCTTCACC AACTTGCCCT TCAAAGTCGG GGGTAACATG CCAGGACGCG 481 GAGCCGGAGA GCGTCTGGCT GTCGATCCGG CCAACTCCAA CATCATCTAC TTTGGTGCTC 541 GCTCAGGAAA CGGCCTCTGG AAGTCTACGG ACGGCGGCGT GACCTTTTCC AAGGTCTCGT 601 CGTTCACGGC AACTGGGACG TACATCCCAG ACCCGAGTGA TTCCAACGGC TACAACAGCG 661 ACAAGCAAGG ACTCATGTGG GTTACGTTCG ACTCAACCAG CAGCACGACC GGGGGAGCCA 721 CGTCTCGTAT CTTTGTTGGC ACGGCTGATA ACATCACTGC TTCAGTCTAT GTGAGCACGA 781 ATGCCGGCTC CACGTGGAGT GCTGTACCGG GGCAGCCAGG GAAATACTTT CCTCACAAGG 841 CGAAACTGCA GCCAGCAGAG AAGGCCTTGT ATCTGACCTA TTCCGATGGC ACAGGGCCGT 901 ATGATGGCAC ACTTGGCTCA GTGTGGAGGT ACGACATTGC AGGGGGAACT TGGAAAGACA 961 TCACCCCTGT CTCTGGATCA GATCTATACT TTGGCTTTGG CGGGCCTTTG GCCTCGATTT 1021 GCAAAAGCCC AGGAACCCTT TGTTGTTGCT TTCTTTGACT CTGGTGACAG ATGCTCAGCT 1081 GTTTCGGTTC GACGACTCTG GACCACCATG GAGCCCCGGA ATTCTTG // |
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| Primer: f1ori_R3 | RDB08298_A6G4b.seq |
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>08298_08298_A6G4_pGL3_RV4_D08_11.ab1 1 CGAGAAGAAG TGCTGGGTTG AGGCTCTCAA GGGCATCGGT CGGAGATCCC GGTGCCTAAT 61 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 181 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC 241 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA 301 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT 361 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG 421 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC 481 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC 541 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG 601 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC 661 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG 721 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA 781 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG 841 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG 901 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG 961 GCCAGACTGG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC 1021 CACGCGGGTT GGGAAATGTT AATTCAGCTC GCCATCGCCG CTTCACTTTT TTCCCGCGTT 1081 TTCGCAGAAA CGTGGCTTGC CTGTCACCAC GCGGAAACGG ATCTTGAATT AGAAGGACA // |
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| Primer: pGL3_RV4 | RDB08298_A6G4c.seq |
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>08298_08298_A6G4_f1ori_R3_H07_22.ab1 1 GGAGGGGCAT CGGATTAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG 61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC 121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA 181 GCTTGTCGAC CACACACTGG TAGTAATAAT CATTCTGCTT CTGGCAGACA TACGGCGCCA 241 CACACTGCGT CGGCCCCGTC CACCCAATCC CTCCGCACTG AGCATAATGA CCGGCGACCC 301 CTGACCCCGT GGGACCGGCG GCCGAGCTGG TCCTCGACGA AGTCACCGTC GAAGCCCGGG 361 TCGTGGATAC AACGCTCGAC CTCAGCGTGG TGCTCGAGCT GGCGGAAGAG GTACTGCTGC 421 TGCTCTGCTT GGTCGACGAG GAAGTCCCGC CCGTGCCGCC GCCGACGGTT CCCTGAGCGT 481 AAAAGACGCC CCGGCCGTTG GTGCCCACGT AGACTTGCCC GGCGGTGCTG CCGCTGCCGG 541 CGACCTTGGT GCTGTCGATG GAGCCGAAGC CCTGGGAGCC CTGGATGTCC GTCCAGGAGG 601 CGCCGCTGTC TCCACTGGCG TAGAGGCGAG CCCCTGACGG GCCGGTGCCG AAGGCATACA 661 GGTTCCAGTT CGAGCCTGAG CCCACACCCA GGGCGATCTG GTAGGTGTTG GTCAGGGCGG 721 TGGAGACTTG GCCAAAGGTC GTGCCCGAGT CTGTGGAGCG GAATATGCCG ACGTCGGTCG 781 AGACATACAA CGTGCCCGCG GTGGTCGGGT GAGCAGCGAT ATCCCGGATC GTCCCTGCGC 841 TGCCCAGCTT GGGCCCGCGC GTGAAGCTGC TGCCGGTGTC CTTGCTGACG TAAAAGGTCG 901 ATCCGGAGCC GGCGTAGAAG ACGCTGTTGG TCTTCTTGTC CGAGGCGATG ACGGCGCCCG 961 CGGGCAGGCT CGAGACGGAG GCAAAGCTGC CCTGGAAACT GCGAGCGCTG CACGCCCGGA 1021 ACGAGCGGGT CGACCAGAGG ATCGTGTCGC CGTCGGCCGA TAGGCCAACG TGCCGCGTTT 1081 CATGGACGTG TCCGGCGCGT AGTCGAATGG CTCCACCGGT CGCGACGGTC GATCGCGGGA 1141 ACCGGAGAT // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl