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pET21c_Tr-cel74a-His

Expression vector of Endoglucanase Cel74a of Trichoderma reesei

Catalog number RDB08298
Resource name pET21c_Tr-cel74a-His
Alternative name pET21c_Tr021-His
Clone info. Biomass collection. the Endoglucanase Cel74a (Endo-1,4-beta-glucanase) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cel74a cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08298 pET21c_Tr-cel74a-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08298z.seq from Depositor
Electronic file PDF RDB08298.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08298_A6G4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08298_A6G4a.seq
>08298_08298_A6G4_T7_20bp_D07_10.ab1
    1 TGGTCGTTCC CATCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCTA
   61 GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG ACCGCCTTTT
  121 CATGGAAGAA CGTCAAGCTC GGCGGCGGCG GCGGCTTCGT CCCCGGCATC ATCTTCCATC
  181 CCAAGACAAA AGGCGTAGCA TATGCACGAA CAGATATTGG CGGGCTGTAC CGCCTCAACG
  241 CCGACGACTC ATGGACCGCC GTCACGGATG GGATTGCTGA TAATGCCGGC TGGCACAACT
  301 GGGGCATCGA CGCTGTTGCG CTTGATCCGC AGGACGATCA AAAGGTGTAT GCCGCAGTCG
  361 GCATGTATAC GAACAGCTGG GATCCGAGTA ATGGAGCCAT CATTCGCTCG TCAGACCGCG
  421 GCGCAACGTG GTCCTTCACC AACTTGCCCT TCAAAGTCGG GGGTAACATG CCAGGACGCG
  481 GAGCCGGAGA GCGTCTGGCT GTCGATCCGG CCAACTCCAA CATCATCTAC TTTGGTGCTC
  541 GCTCAGGAAA CGGCCTCTGG AAGTCTACGG ACGGCGGCGT GACCTTTTCC AAGGTCTCGT
  601 CGTTCACGGC AACTGGGACG TACATCCCAG ACCCGAGTGA TTCCAACGGC TACAACAGCG
  661 ACAAGCAAGG ACTCATGTGG GTTACGTTCG ACTCAACCAG CAGCACGACC GGGGGAGCCA
  721 CGTCTCGTAT CTTTGTTGGC ACGGCTGATA ACATCACTGC TTCAGTCTAT GTGAGCACGA
  781 ATGCCGGCTC CACGTGGAGT GCTGTACCGG GGCAGCCAGG GAAATACTTT CCTCACAAGG
  841 CGAAACTGCA GCCAGCAGAG AAGGCCTTGT ATCTGACCTA TTCCGATGGC ACAGGGCCGT
  901 ATGATGGCAC ACTTGGCTCA GTGTGGAGGT ACGACATTGC AGGGGGAACT TGGAAAGACA
  961 TCACCCCTGT CTCTGGATCA GATCTATACT TTGGCTTTGG CGGGCCTTTG GCCTCGATTT
 1021 GCAAAAGCCC AGGAACCCTT TGTTGTTGCT TTCTTTGACT CTGGTGACAG ATGCTCAGCT
1081 GTTTCGGTTC GACGACTCTG GACCACCATG GAGCCCCGGA ATTCTTG
//
Primer: f1ori_R3 RDB08298_A6G4b.seq
>08298_08298_A6G4_pGL3_RV4_D08_11.ab1
    1 CGAGAAGAAG TGCTGGGTTG AGGCTCTCAA GGGCATCGGT CGGAGATCCC GGTGCCTAAT
   61 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
  121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
  181 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC
  241 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA
  301 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT
  361 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG
  421 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC
  481 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC
  541 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG
  601 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC
  661 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG
  721 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA
  781 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG
  841 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG
  901 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG
  961 GCCAGACTGG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC
 1021 CACGCGGGTT GGGAAATGTT AATTCAGCTC GCCATCGCCG CTTCACTTTT TTCCCGCGTT
1081 TTCGCAGAAA CGTGGCTTGC CTGTCACCAC GCGGAAACGG ATCTTGAATT AGAAGGACA
//
Primer: pGL3_RV4 RDB08298_A6G4c.seq
>08298_08298_A6G4_f1ori_R3_H07_22.ab1
    1 GGAGGGGCAT CGGATTAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG
   61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
  121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA
  181 GCTTGTCGAC CACACACTGG TAGTAATAAT CATTCTGCTT CTGGCAGACA TACGGCGCCA
  241 CACACTGCGT CGGCCCCGTC CACCCAATCC CTCCGCACTG AGCATAATGA CCGGCGACCC
  301 CTGACCCCGT GGGACCGGCG GCCGAGCTGG TCCTCGACGA AGTCACCGTC GAAGCCCGGG
  361 TCGTGGATAC AACGCTCGAC CTCAGCGTGG TGCTCGAGCT GGCGGAAGAG GTACTGCTGC
  421 TGCTCTGCTT GGTCGACGAG GAAGTCCCGC CCGTGCCGCC GCCGACGGTT CCCTGAGCGT
  481 AAAAGACGCC CCGGCCGTTG GTGCCCACGT AGACTTGCCC GGCGGTGCTG CCGCTGCCGG
  541 CGACCTTGGT GCTGTCGATG GAGCCGAAGC CCTGGGAGCC CTGGATGTCC GTCCAGGAGG
  601 CGCCGCTGTC TCCACTGGCG TAGAGGCGAG CCCCTGACGG GCCGGTGCCG AAGGCATACA
  661 GGTTCCAGTT CGAGCCTGAG CCCACACCCA GGGCGATCTG GTAGGTGTTG GTCAGGGCGG
  721 TGGAGACTTG GCCAAAGGTC GTGCCCGAGT CTGTGGAGCG GAATATGCCG ACGTCGGTCG
  781 AGACATACAA CGTGCCCGCG GTGGTCGGGT GAGCAGCGAT ATCCCGGATC GTCCCTGCGC
  841 TGCCCAGCTT GGGCCCGCGC GTGAAGCTGC TGCCGGTGTC CTTGCTGACG TAAAAGGTCG
  901 ATCCGGAGCC GGCGTAGAAG ACGCTGTTGG TCTTCTTGTC CGAGGCGATG ACGGCGCCCG
  961 CGGGCAGGCT CGAGACGGAG GCAAAGCTGC CCTGGAAACT GCGAGCGCTG CACGCCCGGA
 1021 ACGAGCGGGT CGACCAGAGG ATCGTGTCGC CGTCGGCCGA TAGGCCAACG TGCCGCGTTT
 1081 CATGGACGTG TCCGGCGCGT AGTCGAATGG CTCCACCGGT CGCGACGGTC GATCGCGGGA
1141 ACCGGAGAT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl