RDB08298 - pET21c_Tr-cel74a-His

Please review the QC test results indicated by check

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pET21c_Tr-cel74a-His (#RDB08298)

Expression vector of Endoglucanase Cel74a of Trichoderma reesei

Alternative name	pET21c_Tr021-His
Clone info.	Biomass collection. the Endoglucanase Cel74a (Endo-1,4-beta-glucanase) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone	pET-21c (Plasmid)
Size of vector backbone	5441
Selectable markers	Amp^r
Gene/insert name	Trichoderma reesei cel74a cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Sequence	RDB08298z.seq
Remarks, protocol and/or map (pdf)	RDB08298.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	理研BRCへの謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08298	pET21c_Tr-cel74a-His	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET21c_Tr-cel74a-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08298).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08298_A6G4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08298_A6G4a.seq
>08298_08298_A6G4_T7_20bp_D07_10.ab1 1 TGGTCGTTCC CATCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCTA 61 GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG ACCGCCTTTT 121 CATGGAAGAA CGTCAAGCTC GGCGGCGGCG GCGGCTTCGT CCCCGGCATC ATCTTCCATC 181 CCAAGACAAA AGGCGTAGCA TATGCACGAA CAGATATTGG CGGGCTGTAC CGCCTCAACG 241 CCGACGACTC ATGGACCGCC GTCACGGATG GGATTGCTGA TAATGCCGGC TGGCACAACT 301 GGGGCATCGA CGCTGTTGCG CTTGATCCGC AGGACGATCA AAAGGTGTAT GCCGCAGTCG 361 GCATGTATAC GAACAGCTGG GATCCGAGTA ATGGAGCCAT CATTCGCTCG TCAGACCGCG 421 GCGCAACGTG GTCCTTCACC AACTTGCCCT TCAAAGTCGG GGGTAACATG CCAGGACGCG 481 GAGCCGGAGA GCGTCTGGCT GTCGATCCGG CCAACTCCAA CATCATCTAC TTTGGTGCTC 541 GCTCAGGAAA CGGCCTCTGG AAGTCTACGG ACGGCGGCGT GACCTTTTCC AAGGTCTCGT 601 CGTTCACGGC AACTGGGACG TACATCCCAG ACCCGAGTGA TTCCAACGGC TACAACAGCG 661 ACAAGCAAGG ACTCATGTGG GTTACGTTCG ACTCAACCAG CAGCACGACC GGGGGAGCCA 721 CGTCTCGTAT CTTTGTTGGC ACGGCTGATA ACATCACTGC TTCAGTCTAT GTGAGCACGA 781 ATGCCGGCTC CACGTGGAGT GCTGTACCGG GGCAGCCAGG GAAATACTTT CCTCACAAGG 841 CGAAACTGCA GCCAGCAGAG AAGGCCTTGT ATCTGACCTA TTCCGATGGC ACAGGGCCGT 901 ATGATGGCAC ACTTGGCTCA GTGTGGAGGT ACGACATTGC AGGGGGAACT TGGAAAGACA 961 TCACCCCTGT CTCTGGATCA GATCTATACT TTGGCTTTGG CGGGCCTTTG GCCTCGATTT 1021 GCAAAAGCCC AGGAACCCTT TGTTGTTGCT TTCTTTGACT CTGGTGACAG ATGCTCAGCT 1081 GTTTCGGTTC GACGACTCTG GACCACCATG GAGCCCCGGA ATTCTTG //
Primer: f1ori_R3 Sequence file: RDB08298_A6G4b.seq
>08298_08298_A6G4_pGL3_RV4_D08_11.ab1 1 CGAGAAGAAG TGCTGGGTTG AGGCTCTCAA GGGCATCGGT CGGAGATCCC GGTGCCTAAT 61 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 181 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC 241 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA 301 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT 361 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG 421 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC 481 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC 541 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG 601 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC 661 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG 721 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA 781 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG 841 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG 901 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG 961 GCCAGACTGG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC 1021 CACGCGGGTT GGGAAATGTT AATTCAGCTC GCCATCGCCG CTTCACTTTT TTCCCGCGTT 1081 TTCGCAGAAA CGTGGCTTGC CTGTCACCAC GCGGAAACGG ATCTTGAATT AGAAGGACA //
Primer: pGL3_RV4 Sequence file: RDB08298_A6G4c.seq
>08298_08298_A6G4_f1ori_R3_H07_22.ab1 1 GGAGGGGCAT CGGATTAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG 61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC 121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA 181 GCTTGTCGAC CACACACTGG TAGTAATAAT CATTCTGCTT CTGGCAGACA TACGGCGCCA 241 CACACTGCGT CGGCCCCGTC CACCCAATCC CTCCGCACTG AGCATAATGA CCGGCGACCC 301 CTGACCCCGT GGGACCGGCG GCCGAGCTGG TCCTCGACGA AGTCACCGTC GAAGCCCGGG 361 TCGTGGATAC AACGCTCGAC CTCAGCGTGG TGCTCGAGCT GGCGGAAGAG GTACTGCTGC 421 TGCTCTGCTT GGTCGACGAG GAAGTCCCGC CCGTGCCGCC GCCGACGGTT CCCTGAGCGT 481 AAAAGACGCC CCGGCCGTTG GTGCCCACGT AGACTTGCCC GGCGGTGCTG CCGCTGCCGG 541 CGACCTTGGT GCTGTCGATG GAGCCGAAGC CCTGGGAGCC CTGGATGTCC GTCCAGGAGG 601 CGCCGCTGTC TCCACTGGCG TAGAGGCGAG CCCCTGACGG GCCGGTGCCG AAGGCATACA 661 GGTTCCAGTT CGAGCCTGAG CCCACACCCA GGGCGATCTG GTAGGTGTTG GTCAGGGCGG 721 TGGAGACTTG GCCAAAGGTC GTGCCCGAGT CTGTGGAGCG GAATATGCCG ACGTCGGTCG 781 AGACATACAA CGTGCCCGCG GTGGTCGGGT GAGCAGCGAT ATCCCGGATC GTCCCTGCGC 841 TGCCCAGCTT GGGCCCGCGC GTGAAGCTGC TGCCGGTGTC CTTGCTGACG TAAAAGGTCG 901 ATCCGGAGCC GGCGTAGAAG ACGCTGTTGG TCTTCTTGTC CGAGGCGATG ACGGCGCCCG 961 CGGGCAGGCT CGAGACGGAG GCAAAGCTGC CCTGGAAACT GCGAGCGCTG CACGCCCGGA 1021 ACGAGCGGGT CGACCAGAGG ATCGTGTCGC CGTCGGCCGA TAGGCCAACG TGCCGCGTTT 1081 CATGGACGTG TCCGGCGCGT AGTCGAATGG CTCCACCGGT CGCGACGGTC GATCGCGGGA 1141 ACCGGAGAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

External Database
Trichoderma reesei cel74a

Reference sequence