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pET21c_Tr-egl3-His (#RDB08290)

Expression vector of Endoglucanase EG-III of Trichoderma reesei

Alternative name pET21c_Tr005-His
Clone info. Biomass collection. the Endoglucanase EG-III (EGLIII, Endo-1,4-beta glucanase) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl3 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
 
Sequence RDB08290z.seq
Remarks, protocol and/or map (pdf) RDB08290.pdf

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RDB08290 pET21c_Tr-egl3-His DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET21c_Tr-egl3-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08290).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08290_A6G4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB08290_A6G4a.seq check
>08290_08290_A6G4_T7_20bp_C07_07.ab1
    1 TACGTCGCTC GCATCTAGAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCT
   61 AGCATGACTG GTGGACAGCA AATGGGTCGG ATCCGAATTC GAGCTCCGTC GACCCAAACC
  121 AGCTGTGACC AGTGGGCAAC CTTCACTGGC AACGGCTACA CAGTCAGCAA CAACCTTTGG
  181 GGAGCATCAG CCGGCTCTGG ATTTGGCTGC GTGACGGCGG TATCGCTCAG CGGCGGGGCC
  241 TCCTGGCACG CAGACTGGCA GTGGTCCGGC GGCCAGAACA ACGTCAAGTC GTACCAGAAC
  301 TCTCAGATTG CCATTCCCCA GAAGAGGACC GTCAACAGCA TCAGCAGCAT GCCCACCACT
  361 GCCAGCTGGA GCTACAGCGG GAGCAACATC CGCGCTAATG TTGCGTATGA CTTGTTCACC
  421 GCAGCCAACC CGAATCATGT CACGTACTCG GGAGACTACG AACTCATGAT CTGGCTTGGC
  481 AAATACGGCG ATATTGGGCC GATTGGGTCC TCACAGGGAA CAGTCAACGT CGGTGGCCAG
  541 AGCTGGACGC TCTACTATGG CTACAACGGA GCCATGCAAG TCTATTCCTT TGTGGCCCAG
  601 ACCAACACTA CCAACTACAG CGGAGATGTC AAGAACTTCT TCAATTATCT CCGAGACAAT
  661 AAAGGATACA ACGCTGCAGG CCAATATGTT CTTAGCTACC AATTTGGTAC CGAGCCCTTC
  721 ACGGGCAGTG GAACTCTGAA CGTCGCATCC TGGACCGCAT CTATCAACGT CGACAAGCTT
  781 GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA
  841 AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
  901 TCTAAACGGG TCTTGAGGGG TTTTTTTGCT GAAAGGAGGA ACTATATCCG GATTGGCGAA
  961 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGGCGGGT GTGGTGGTTA CGCGCAGCGT
 1021 GACCGCTACA CTTGCCAGCG CCCCTAGCGC CCGCTCGTTT CGCTTTCGTC CGTTCGTTTT
1081 CTCGCCACGT TCGACTGCTT ACCCCGGTTC AGCTCTAAAT CAGGGGG
//
Primer: pGL3_RV4
Sequence file: RDB08290_A6G4b.seq check
>08290_08290_A6G4_f1ori_R3_G07_19.ab1
    1 GGATGCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGAGTGCG GCCGCAAGCT
  181 TGTCGACGTT GATAGATGCG GTCCAGGATG CGACGTTCAG AGTTCCACTG CCCGTGAAGG
  241 GCTCGGTACC AAATTGGTAG CTAAGAACAT ATTGGCCTGC AGCGTTGTAT CCTTTATTGT
  301 CTCGGAGATA ATTGAAGAAG TTCTTGACAT CTCCGCTGTA GTTGGTAGTG TTGGTCTGGG
  361 CCACAAAGGA ATAGACTTGC ATGGCTCCGT TGTAGCCATA GTAGAGCGTC CAGCTCTGGC
  421 CACCGACGTT GACTGTTCCC TGTGAGGACC CAATCGGCCC AATATCGCCG TATTTGCCAA
  481 GCCAGATCAT GAGTTCGTAG TCTCCCGAGT ACGTGACATG ATTCGGGTTG GCTGCGGTGA
  541 ACAAGTCATA CGCAACATTA GCGCGGATGT TGCTCCCGCT GTAGCTCCAG CTGGCAGTGG
  601 TGGGCATGCT GCTGATGCTG TTGACGGTCC TCTTCTGGGG AATGGCAATC TGAGAGTTCT
  661 GGTACGACTT GACGTTGTTC TGGCCGCCGG ACCACTGCCA GTCTGCGTGC CAGGAGGCCC
  721 CGCCGCTGAG CGATACCGCC GTCACGCAGC CAAATCCAGA GCCGGCTGAT GCTCCCCAAA
  781 GGTTGTTGCT GACTGTGTAG CCGTTGCCAG TGAAGGTTGC CCACTGGTCA CAGCTGGTTT
  841 GGGTCGACGG AGCTCGAATT CGGATCCGAC CCATTTGCTG TCCACCAGTC ATGCTAGCCA
  901 TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC
  961 TCACAATTCC CCTATAGTGA GTCGTATTTA ATTTCGCGGG ATCGAGATCT CGATCCTCTA
 1021 CGCCGGGACG CATCGTGGCC GGGCATCAAC CGGGCGCCAC AGTGCGTAGC TGGCGGCTAT
1081 ATCGCCGGAC TCACGATTGA GAGATCGGGC TCGCCACTTC GGCCCTTCAA TGGAAGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles