Sequencing and PCR primers for primer information.
Expression vector of Endoglucanase EG-III of Trichoderma reesei
| Catalog number | RDB08290 |
|---|---|
| Resource name | pET21c_Tr-egl3-His |
| Alternative name | pET21c_Tr005-His |
| Clone info. | Biomass collection. the Endoglucanase EG-III (EGLIII, Endo-1,4-beta glucanase) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c. |
| Vector backbone | pET-21c (Plasmid) |
| Size of vector backbone | 5441 |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei egl3 cDNA |
| Depositor | DNA Bank, | Kishikawa, Shotaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08290 | pET21c_Tr-egl3-His | DNA solution |
Electronic file
| Electronic file | Sequence RDB08290z.seq from Depositor |
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| Electronic file | PDF RDB08290.pdf from Depositor |
Featured content
| Featured content | Biomass Collection (English text) |
|---|---|
| Featured content | Biomass Collection (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08290_A6G4p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB08290_A6G4a.seq |
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>08290_08290_A6G4_T7_20bp_C07_07.ab1 1 TACGTCGCTC GCATCTAGAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCT 61 AGCATGACTG GTGGACAGCA AATGGGTCGG ATCCGAATTC GAGCTCCGTC GACCCAAACC 121 AGCTGTGACC AGTGGGCAAC CTTCACTGGC AACGGCTACA CAGTCAGCAA CAACCTTTGG 181 GGAGCATCAG CCGGCTCTGG ATTTGGCTGC GTGACGGCGG TATCGCTCAG CGGCGGGGCC 241 TCCTGGCACG CAGACTGGCA GTGGTCCGGC GGCCAGAACA ACGTCAAGTC GTACCAGAAC 301 TCTCAGATTG CCATTCCCCA GAAGAGGACC GTCAACAGCA TCAGCAGCAT GCCCACCACT 361 GCCAGCTGGA GCTACAGCGG GAGCAACATC CGCGCTAATG TTGCGTATGA CTTGTTCACC 421 GCAGCCAACC CGAATCATGT CACGTACTCG GGAGACTACG AACTCATGAT CTGGCTTGGC 481 AAATACGGCG ATATTGGGCC GATTGGGTCC TCACAGGGAA CAGTCAACGT CGGTGGCCAG 541 AGCTGGACGC TCTACTATGG CTACAACGGA GCCATGCAAG TCTATTCCTT TGTGGCCCAG 601 ACCAACACTA CCAACTACAG CGGAGATGTC AAGAACTTCT TCAATTATCT CCGAGACAAT 661 AAAGGATACA ACGCTGCAGG CCAATATGTT CTTAGCTACC AATTTGGTAC CGAGCCCTTC 721 ACGGGCAGTG GAACTCTGAA CGTCGCATCC TGGACCGCAT CTATCAACGT CGACAAGCTT 781 GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA 841 AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 901 TCTAAACGGG TCTTGAGGGG TTTTTTTGCT GAAAGGAGGA ACTATATCCG GATTGGCGAA 961 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGGCGGGT GTGGTGGTTA CGCGCAGCGT 1021 GACCGCTACA CTTGCCAGCG CCCCTAGCGC CCGCTCGTTT CGCTTTCGTC CGTTCGTTTT 1081 CTCGCCACGT TCGACTGCTT ACCCCGGTTC AGCTCTAAAT CAGGGGG // |
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| Primer: pGL3_RV4 | RDB08290_A6G4b.seq |
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>08290_08290_A6G4_f1ori_R3_G07_19.ab1 1 GGATGCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGAGTGCG GCCGCAAGCT 181 TGTCGACGTT GATAGATGCG GTCCAGGATG CGACGTTCAG AGTTCCACTG CCCGTGAAGG 241 GCTCGGTACC AAATTGGTAG CTAAGAACAT ATTGGCCTGC AGCGTTGTAT CCTTTATTGT 301 CTCGGAGATA ATTGAAGAAG TTCTTGACAT CTCCGCTGTA GTTGGTAGTG TTGGTCTGGG 361 CCACAAAGGA ATAGACTTGC ATGGCTCCGT TGTAGCCATA GTAGAGCGTC CAGCTCTGGC 421 CACCGACGTT GACTGTTCCC TGTGAGGACC CAATCGGCCC AATATCGCCG TATTTGCCAA 481 GCCAGATCAT GAGTTCGTAG TCTCCCGAGT ACGTGACATG ATTCGGGTTG GCTGCGGTGA 541 ACAAGTCATA CGCAACATTA GCGCGGATGT TGCTCCCGCT GTAGCTCCAG CTGGCAGTGG 601 TGGGCATGCT GCTGATGCTG TTGACGGTCC TCTTCTGGGG AATGGCAATC TGAGAGTTCT 661 GGTACGACTT GACGTTGTTC TGGCCGCCGG ACCACTGCCA GTCTGCGTGC CAGGAGGCCC 721 CGCCGCTGAG CGATACCGCC GTCACGCAGC CAAATCCAGA GCCGGCTGAT GCTCCCCAAA 781 GGTTGTTGCT GACTGTGTAG CCGTTGCCAG TGAAGGTTGC CCACTGGTCA CAGCTGGTTT 841 GGGTCGACGG AGCTCGAATT CGGATCCGAC CCATTTGCTG TCCACCAGTC ATGCTAGCCA 901 TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC 961 TCACAATTCC CCTATAGTGA GTCGTATTTA ATTTCGCGGG ATCGAGATCT CGATCCTCTA 1021 CGCCGGGACG CATCGTGGCC GGGCATCAAC CGGGCGCCAC AGTGCGTAGC TGGCGGCTAT 1081 ATCGCCGGAC TCACGATTGA GAGATCGGGC TCGCCACTTC GGCCCTTCAA TGGAAGGC // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl