Sequencing and PCR primers for primer information.
Expression vector of Endoglucanase EG-I of Trichoderma reesei
| Catalog number | RDB08288 |
|---|---|
| Resource name | pET21c_Tr-egl1-His |
| Alternative name | pET21c_Tr003-His |
| Clone info. | Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c. |
| Vector backbone | pET-21c (Plasmid) |
| Size of vector backbone | 5441bp |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei egl1 cDNA |
| Depositor | DNA Bank, | Kishikawa, Shotaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08288 | pET21c_Tr-egl1-His | DNA solution |
Electronic file
| Electronic file | Sequence RDB08288z.seq from Depositor |
|---|---|
| Electronic file | PDF RDB08288.pdf from Depositor |
Featured content
| Featured content | Biomass Collection (English text) |
|---|---|
| Featured content | Biomass Collection (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08288_A6G4p1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB08288_A6G4a.seq |
|---|---|
|
>08288_08288_A6G4_T7_20bp_A07_01.ab1 1 TGCTCATTCC ATCTAGAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTAG 61 CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA CCCAGCAACC 121 GGGTACCAGC ACCCCCGAGG TCCATCCCAA GTTGACAACC TACAAGTGTA CAAAGTCCGG 181 GGGGTGCGTG GCCCAGGACA CCTCGGTGGT CCTTGACTGG AACTACCGCT GGATGCACGA 241 CGCAAACTAC AACTCGTGCA CCGTCAACGG CGGCGTCAAC ACCACGCTCT GCCCTGACGA 301 GGCGACCTGT GGCAAGAACT GCTTCATCGA GGGCGTCGAC TACGCCGCCT CGGGCGTCAC 361 GACCTCGGGC AGCAGCCTCA CCATGAACCA GTACATGCCC AGCAGCTCTG GCGGCTACAG 421 CAGCGTCTCT CCTCGGCTGT ATCTCCTGGA CTCTGACGGT GAGTACGTGA TGCTGAAGCT 481 CAACGGCCAG GAGCTGAGCT TCGACGTCGA CCTCTCTGCT CTGCCGTGTG GAGAGAACGG 541 CTCGCTCTAC CTGTCTCAGA TGGACGAGAA CGGGGGCGCC AACCAGTATA ACACGGCCGG 601 TGCCAACTAC GGGAGCGGCT ACTGCGATGC TCAGTGCCCC GTCCAGACAT GGAGGAACGG 661 CACCCTCAAC ACTAGCCACC AGGGCTTCTG CTGCAACGAG ATGGATATCC TGGAGGGCAA 721 CTCGAGGGCG AATGCCTTGA CCCCTCACTC TTGCACGGCC ACGGCCTGCG ACTCTGCCGG 781 TTGCGGCTTC AACCCCTATG GCAGCGGCTA CAAAAGCTAC TACGGCCCCG GAGATACCGT 841 TGACACCTCC AAGACCTTCA CCATCATCAC CCAGTTCAAC ACGGACAACG GCTCGCCCTC 901 GGGCAACCTT GTGAGCATCA CCCGCAAGTA CCAGCAAAAC GGCGTCGACA TCCCCAGCGC 961 CCAGCCCGGG CGGCGACACC ATCTCGTCCC TGCCCGTCCC GCCTCAGCCT ACGGCGGGCC 1021 TCGCCACCCA TGGGCAACGC CCCTGAGCAG CGGGCATGGT GCTTCGTTGA TCAGCATTTG 1081 CACGACACAG GCAGTACATG GACCTGGCCT TCGACAGGCG GGCCAACCGC CCGGACCCCC 1141 CTGCA // |
|
| Primer: f1ori_R3 | RDB08288_A6G4b.seq |
|
>08288_08288_A6G4_f1ori_R3_E07_13.ab1 1 GCATGGCATC GGATTAGTTG CTCCTTTCAG CAGAAACCCC TCAAGACCCG TTTAGAGGCC 61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC 181 TTGTCGACAA GGCATTGCGA GTAGTAGTCG TTGCTATACT GGCACGTAGT GCCCGACGTG 241 CACGTCTTGC ACCCGCTGTA CCCAATGCCA CCGCACTGCC CCCAGTGAGT CTGCGTGCAG 301 CTCGGGCTGC TCGAAGTCGT CGAGCTCCTC CGTGTAGTCG AAAACGTCGT GCTGGACGCA 361 GGCGGGGGCG GGGGCGCAGT CGAGTTCGTA GTAGACCCAA TGTCTCCCCA GCGGATGTTG 421 GAGAAGACGA CGTGCGTGTT GGGGTTGTTG GCCAGGATGT TGGATGGGTT GCCCTCGGTG 481 CTGCTGCAGG GGCCGGCGTT GCCGCTGTCG AGCCAGTTCA TGTACTGGCT GTTGTCGTTC 541 CAAATGCTGA ACACGAGCAC CATGCCGCTG CTCAGGGCCT TGCCCATGGT GGCGAGGCCG 601 CCGTAGGCTG AGGCGGACGG GCAGGACGAG ATGGTGTCGC CGCCGGGCTG GGCGCTGGGG 661 ATGTCGACGC CGTTTTGCTG GTACTTGCGG GTGATGCTCA CAAGGTTGCC CGAGGGCGAG 721 CCGTTGTCCG TGTTGAACTG GGTGATGATG GTGAAGGTCT TGGAGGTGTC AACGGTATCT 781 CCGGGGCCGT AGTAGCTTTT GTAGCCGCTG CCATAGGGGT TGAAGCCGCA ACCGGCAGAG 841 TCGCAGGCCG TGGCCGTGCA AGAGTGAGGG GTCAAGGCAT TCGCCCTCGA GTTGCCCTCC 901 AGGATATCCA TCTCGTTGCA GCAGAAGCCC TGGTGGCTAG TGTTGAGGGT GCCGTTCCTC 961 CATGTCTGGA CGGGGCACTG AGCATCGCAG TAGCCGCTCC CGTAGTTTGG CACCGGCCGT 1021 GTTATACTGG GTTTGGCGCC CCCGTTCTCG TCCCATCTGA GACAGTAGAG CGAGCCCGAT 1081 TCTTCTCCAC ACGCCAGAGG CAGAGAAGGT CGACGATCGA AGGCCTCCAG CCTTCCTTGG 1141 CCGT // |
|
| Primer: pGL3_RV4 | RDB08288_A6G4c.seq |
|
>08288_08288_A6G4_pGL3_RV4_A08_02.ab1 1 GTAACTATGG GTTGAGCTCT CAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT 61 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 121 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG 181 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC 241 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG 301 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC 361 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC 421 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG 481 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT 541 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT 601 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC 661 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA 721 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA 781 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC 841 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT 901 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG 961 GAGGTGGCAA CGCCCAATCA GCAACGACTG TTTGCCCGCC CAGTTTGTTG TGCCACGCGG 1021 GTTTGGGAAT GTAAATTTCA GCTCGCCATC GCGCTTCCAC TTTTTCCCGG CGGTTTTCGC 1081 AGAAACGTTG GCCTACTGGC TCAACCAACG CGGGCAAACG GATCTGAATA AGGAGACCAC 1141 CCGGGCT // |
|
Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl