Expression vector of Endoglucanase EG-I of Trichoderma reesei
Alternative name | pET21c_Tr003-His |
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Clone info. | Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c. |
Vector backbone | pET-21c (Plasmid) |
Size of vector backbone | 5441bp |
Selectable markers | Amp^r |
Gene/insert name | Trichoderma reesei egl1 cDNA |
Depositor|Developer | DNA Bank, | Kishikawa, Shotaro | |
Sequence | RDB08288z.seq | Remarks, protocol and/or map (pdf) | RDB08288.pdf |
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必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 理研BRCへの謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08288 | pET21c_Tr-egl1-His | DNA solution |
Materials & Methods section:
The pET21c_Tr-egl1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08288). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08288_A6G4p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: T7_20bp Sequence file: RDB08288_A6G4a.seq |
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>08288_08288_A6G4_T7_20bp_A07_01.ab1 1 TGCTCATTCC ATCTAGAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTAG 61 CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA CCCAGCAACC 121 GGGTACCAGC ACCCCCGAGG TCCATCCCAA GTTGACAACC TACAAGTGTA CAAAGTCCGG 181 GGGGTGCGTG GCCCAGGACA CCTCGGTGGT CCTTGACTGG AACTACCGCT GGATGCACGA 241 CGCAAACTAC AACTCGTGCA CCGTCAACGG CGGCGTCAAC ACCACGCTCT GCCCTGACGA 301 GGCGACCTGT GGCAAGAACT GCTTCATCGA GGGCGTCGAC TACGCCGCCT CGGGCGTCAC 361 GACCTCGGGC AGCAGCCTCA CCATGAACCA GTACATGCCC AGCAGCTCTG GCGGCTACAG 421 CAGCGTCTCT CCTCGGCTGT ATCTCCTGGA CTCTGACGGT GAGTACGTGA TGCTGAAGCT 481 CAACGGCCAG GAGCTGAGCT TCGACGTCGA CCTCTCTGCT CTGCCGTGTG GAGAGAACGG 541 CTCGCTCTAC CTGTCTCAGA TGGACGAGAA CGGGGGCGCC AACCAGTATA ACACGGCCGG 601 TGCCAACTAC GGGAGCGGCT ACTGCGATGC TCAGTGCCCC GTCCAGACAT GGAGGAACGG 661 CACCCTCAAC ACTAGCCACC AGGGCTTCTG CTGCAACGAG ATGGATATCC TGGAGGGCAA 721 CTCGAGGGCG AATGCCTTGA CCCCTCACTC TTGCACGGCC ACGGCCTGCG ACTCTGCCGG 781 TTGCGGCTTC AACCCCTATG GCAGCGGCTA CAAAAGCTAC TACGGCCCCG GAGATACCGT 841 TGACACCTCC AAGACCTTCA CCATCATCAC CCAGTTCAAC ACGGACAACG GCTCGCCCTC 901 GGGCAACCTT GTGAGCATCA CCCGCAAGTA CCAGCAAAAC GGCGTCGACA TCCCCAGCGC 961 CCAGCCCGGG CGGCGACACC ATCTCGTCCC TGCCCGTCCC GCCTCAGCCT ACGGCGGGCC 1021 TCGCCACCCA TGGGCAACGC CCCTGAGCAG CGGGCATGGT GCTTCGTTGA TCAGCATTTG 1081 CACGACACAG GCAGTACATG GACCTGGCCT TCGACAGGCG GGCCAACCGC CCGGACCCCC 1141 CTGCA // |
Primer: f1ori_R3 Sequence file: RDB08288_A6G4b.seq |
>08288_08288_A6G4_f1ori_R3_E07_13.ab1 1 GCATGGCATC GGATTAGTTG CTCCTTTCAG CAGAAACCCC TCAAGACCCG TTTAGAGGCC 61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC 181 TTGTCGACAA GGCATTGCGA GTAGTAGTCG TTGCTATACT GGCACGTAGT GCCCGACGTG 241 CACGTCTTGC ACCCGCTGTA CCCAATGCCA CCGCACTGCC CCCAGTGAGT CTGCGTGCAG 301 CTCGGGCTGC TCGAAGTCGT CGAGCTCCTC CGTGTAGTCG AAAACGTCGT GCTGGACGCA 361 GGCGGGGGCG GGGGCGCAGT CGAGTTCGTA GTAGACCCAA TGTCTCCCCA GCGGATGTTG 421 GAGAAGACGA CGTGCGTGTT GGGGTTGTTG GCCAGGATGT TGGATGGGTT GCCCTCGGTG 481 CTGCTGCAGG GGCCGGCGTT GCCGCTGTCG AGCCAGTTCA TGTACTGGCT GTTGTCGTTC 541 CAAATGCTGA ACACGAGCAC CATGCCGCTG CTCAGGGCCT TGCCCATGGT GGCGAGGCCG 601 CCGTAGGCTG AGGCGGACGG GCAGGACGAG ATGGTGTCGC CGCCGGGCTG GGCGCTGGGG 661 ATGTCGACGC CGTTTTGCTG GTACTTGCGG GTGATGCTCA CAAGGTTGCC CGAGGGCGAG 721 CCGTTGTCCG TGTTGAACTG GGTGATGATG GTGAAGGTCT TGGAGGTGTC AACGGTATCT 781 CCGGGGCCGT AGTAGCTTTT GTAGCCGCTG CCATAGGGGT TGAAGCCGCA ACCGGCAGAG 841 TCGCAGGCCG TGGCCGTGCA AGAGTGAGGG GTCAAGGCAT TCGCCCTCGA GTTGCCCTCC 901 AGGATATCCA TCTCGTTGCA GCAGAAGCCC TGGTGGCTAG TGTTGAGGGT GCCGTTCCTC 961 CATGTCTGGA CGGGGCACTG AGCATCGCAG TAGCCGCTCC CGTAGTTTGG CACCGGCCGT 1021 GTTATACTGG GTTTGGCGCC CCCGTTCTCG TCCCATCTGA GACAGTAGAG CGAGCCCGAT 1081 TCTTCTCCAC ACGCCAGAGG CAGAGAAGGT CGACGATCGA AGGCCTCCAG CCTTCCTTGG 1141 CCGT // |
Primer: pGL3_RV4 Sequence file: RDB08288_A6G4c.seq |
>08288_08288_A6G4_pGL3_RV4_A08_02.ab1 1 GTAACTATGG GTTGAGCTCT CAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT 61 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 121 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG 181 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC 241 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG 301 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC 361 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC 421 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG 481 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT 541 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT 601 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC 661 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA 721 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA 781 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC 841 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT 901 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG 961 GAGGTGGCAA CGCCCAATCA GCAACGACTG TTTGCCCGCC CAGTTTGTTG TGCCACGCGG 1021 GTTTGGGAAT GTAAATTTCA GCTCGCCATC GCGCTTCCAC TTTTTCCCGG CGGTTTTCGC 1081 AGAAACGTTG GCCTACTGGC TCAACCAACG CGGGCAAACG GATCTGAATA AGGAGACCAC 1141 CCGGGCT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
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