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pET21c_Tr-egl1-His

Expression vector of Endoglucanase EG-I of Trichoderma reesei

Catalog number RDB08288
Resource name pET21c_Tr-egl1-His
Alternative name pET21c_Tr003-His
Clone info. Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl1 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08288 pET21c_Tr-egl1-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08288z.seq from Depositor
Electronic file PDF RDB08288.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08288_A6G4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08288_A6G4a.seq
>08288_08288_A6G4_T7_20bp_A07_01.ab1
    1 TGCTCATTCC ATCTAGAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTAG
   61 CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA CCCAGCAACC
  121 GGGTACCAGC ACCCCCGAGG TCCATCCCAA GTTGACAACC TACAAGTGTA CAAAGTCCGG
  181 GGGGTGCGTG GCCCAGGACA CCTCGGTGGT CCTTGACTGG AACTACCGCT GGATGCACGA
  241 CGCAAACTAC AACTCGTGCA CCGTCAACGG CGGCGTCAAC ACCACGCTCT GCCCTGACGA
  301 GGCGACCTGT GGCAAGAACT GCTTCATCGA GGGCGTCGAC TACGCCGCCT CGGGCGTCAC
  361 GACCTCGGGC AGCAGCCTCA CCATGAACCA GTACATGCCC AGCAGCTCTG GCGGCTACAG
  421 CAGCGTCTCT CCTCGGCTGT ATCTCCTGGA CTCTGACGGT GAGTACGTGA TGCTGAAGCT
  481 CAACGGCCAG GAGCTGAGCT TCGACGTCGA CCTCTCTGCT CTGCCGTGTG GAGAGAACGG
  541 CTCGCTCTAC CTGTCTCAGA TGGACGAGAA CGGGGGCGCC AACCAGTATA ACACGGCCGG
  601 TGCCAACTAC GGGAGCGGCT ACTGCGATGC TCAGTGCCCC GTCCAGACAT GGAGGAACGG
  661 CACCCTCAAC ACTAGCCACC AGGGCTTCTG CTGCAACGAG ATGGATATCC TGGAGGGCAA
  721 CTCGAGGGCG AATGCCTTGA CCCCTCACTC TTGCACGGCC ACGGCCTGCG ACTCTGCCGG
  781 TTGCGGCTTC AACCCCTATG GCAGCGGCTA CAAAAGCTAC TACGGCCCCG GAGATACCGT
  841 TGACACCTCC AAGACCTTCA CCATCATCAC CCAGTTCAAC ACGGACAACG GCTCGCCCTC
  901 GGGCAACCTT GTGAGCATCA CCCGCAAGTA CCAGCAAAAC GGCGTCGACA TCCCCAGCGC
  961 CCAGCCCGGG CGGCGACACC ATCTCGTCCC TGCCCGTCCC GCCTCAGCCT ACGGCGGGCC
 1021 TCGCCACCCA TGGGCAACGC CCCTGAGCAG CGGGCATGGT GCTTCGTTGA TCAGCATTTG
 1081 CACGACACAG GCAGTACATG GACCTGGCCT TCGACAGGCG GGCCAACCGC CCGGACCCCC
1141 CTGCA
//
Primer: f1ori_R3 RDB08288_A6G4b.seq
>08288_08288_A6G4_f1ori_R3_E07_13.ab1
    1 GCATGGCATC GGATTAGTTG CTCCTTTCAG CAGAAACCCC TCAAGACCCG TTTAGAGGCC
   61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG
  121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC
  181 TTGTCGACAA GGCATTGCGA GTAGTAGTCG TTGCTATACT GGCACGTAGT GCCCGACGTG
  241 CACGTCTTGC ACCCGCTGTA CCCAATGCCA CCGCACTGCC CCCAGTGAGT CTGCGTGCAG
  301 CTCGGGCTGC TCGAAGTCGT CGAGCTCCTC CGTGTAGTCG AAAACGTCGT GCTGGACGCA
  361 GGCGGGGGCG GGGGCGCAGT CGAGTTCGTA GTAGACCCAA TGTCTCCCCA GCGGATGTTG
  421 GAGAAGACGA CGTGCGTGTT GGGGTTGTTG GCCAGGATGT TGGATGGGTT GCCCTCGGTG
  481 CTGCTGCAGG GGCCGGCGTT GCCGCTGTCG AGCCAGTTCA TGTACTGGCT GTTGTCGTTC
  541 CAAATGCTGA ACACGAGCAC CATGCCGCTG CTCAGGGCCT TGCCCATGGT GGCGAGGCCG
  601 CCGTAGGCTG AGGCGGACGG GCAGGACGAG ATGGTGTCGC CGCCGGGCTG GGCGCTGGGG
  661 ATGTCGACGC CGTTTTGCTG GTACTTGCGG GTGATGCTCA CAAGGTTGCC CGAGGGCGAG
  721 CCGTTGTCCG TGTTGAACTG GGTGATGATG GTGAAGGTCT TGGAGGTGTC AACGGTATCT
  781 CCGGGGCCGT AGTAGCTTTT GTAGCCGCTG CCATAGGGGT TGAAGCCGCA ACCGGCAGAG
  841 TCGCAGGCCG TGGCCGTGCA AGAGTGAGGG GTCAAGGCAT TCGCCCTCGA GTTGCCCTCC
  901 AGGATATCCA TCTCGTTGCA GCAGAAGCCC TGGTGGCTAG TGTTGAGGGT GCCGTTCCTC
  961 CATGTCTGGA CGGGGCACTG AGCATCGCAG TAGCCGCTCC CGTAGTTTGG CACCGGCCGT
 1021 GTTATACTGG GTTTGGCGCC CCCGTTCTCG TCCCATCTGA GACAGTAGAG CGAGCCCGAT
 1081 TCTTCTCCAC ACGCCAGAGG CAGAGAAGGT CGACGATCGA AGGCCTCCAG CCTTCCTTGG
1141 CCGT
//
Primer: pGL3_RV4 RDB08288_A6G4c.seq
>08288_08288_A6G4_pGL3_RV4_A08_02.ab1
    1 GTAACTATGG GTTGAGCTCT CAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT
   61 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
  121 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG
  181 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC
  241 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG
  301 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC
  361 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC
  421 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG
  481 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT
  541 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT
  601 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC
  661 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA
  721 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA
  781 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC
  841 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT
  901 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG
  961 GAGGTGGCAA CGCCCAATCA GCAACGACTG TTTGCCCGCC CAGTTTGTTG TGCCACGCGG
 1021 GTTTGGGAAT GTAAATTTCA GCTCGCCATC GCGCTTCCAC TTTTTCCCGG CGGTTTTCGC
 1081 AGAAACGTTG GCCTACTGGC TCAACCAACG CGGGCAAACG GATCTGAATA AGGAGACCAC
1141 CCGGGCT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl