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pGL4-phE2F1 (#RDB07810)

Promoter collection, Human E2F1 promoter

Alternative name U3022-2
Clone info. PCR amplified human E2F1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 33687850 to 33686378(NC_000020.11); relatively -1465 to +8, where +1 corresponds to 1 nt of NM_005225.3.
Comment PCR cloning, forward primer :aaggggacatggcattgtta; reverse primer : aaagtcccggccacttttac.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human E2F transcription factor 1 genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07810 pGL4-phE2F1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phE2F1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07810).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07810_A4Hb.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07810_A4Hba.seq check
>07810_07810_A4Hb_pGL4-4174F_E07_13.ab1
    1 AATTGTGTCC AGAAATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
   61 GATAAGGGGA CATGGCATTG TTAAAATGCA GATTCTTGGG CACCTAGAGA TTTTAAATTG
  121 GCAGGGCCAG GGTGGGCCCA AAATTAGCAA GTGACCACGT GGTTCTGAAG CCAGTGGCCT
  181 AAGGACCACC CTTGCAGAAC CGTGGTCTCC TTGTCACAGT CTAGGCAGCC TCTGGCTTAG
  241 CCTCTGTTTC TTTCATAACC TTTCTCAGCG CCTGCTCTGG GCCAGACCAG TGTTGGGAGG
  301 AGTCGCTACT GAGCTCCTAG ATTGGCAGGG GAGGCAGATG GAGAAAAGGA GTGTGTGTGG
  361 TCAGCATTGG AGCAGAGGCA GCAGTGGGCA ATAGAGGAAG TGAGTAAATC CTTGGGAGGG
  421 CTCCCTAGAA GTGATGTGTT TTCTTTTTTT GTTTTAGAGA CAGGATCTCG CTCTGTCGCC
  481 CAGGCTGGTG TGCAGTGGCA TGATCATAGC TCACTGCAGC CTCGACTTCT CGGGCTCAAG
  541 CAATCCTCCC ACCTCAGCCT CCCAAGTAGC TGGGACTACG GGCACACGCC ACCATGCCTG
  601 GCTAATTTTT GTATTTTTTG TAGAGATGGG TCTTCACCAT GTTGATCAGG CTGGTCTCGA
  661 ACTCCTGGGC TCATGCGATC CACCCCGCCA GCTGATTACA GGGATTCCGG TGGTGAGCCA
  721 CCGCGCCCAG ACGCCACTTC ATCGTATTGT AAACGTCTGT TACCTTTCTG TTCCCCTGTC
  781 TACTGGACTG TGAGCTCCTT AGGGCCACGA ATTGAGGATG GGGCACAGAG CAAGCTCTCC
  841 AAACGTTTGT TGAATGAGTG AGGGAATGAA TGAGTTCAAG CAGATGCTAT ACGTTGGCTG
  901 TTGGAGATTT TGGCTAAAAT GGGACTTGCA GGAAAGCCCG ACGTCCCCCT CGCCATTTCC
  961 AGGCACCGCT CTTCAGCTTG GGCTCTGGGT GAGCGGGATA GGGCTGGGTG CAGGATTAGG
 1021 ATAATGTCAT GGGTGAGCAA GTTGGAGGAT GAGAGTGGCT GATGCTGGCT GTGTACTGAT
1081 GATCTTGAAA GAGAGGACAG TCTCTGGCAA TCTTAAGCCT TGAAGGCCTT GT
//
Primer: pGL4-136R
Sequence file: RDB07810_A4Hbb.seq check
>07810_07810_A4Hb_pGL4-136R_F07_16.ab1
    1 GTCTCTTTGT TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGCG CATTGCCAAG
   61 CTTGGCCGCC GAGGCCAGAT CTTGATAAAG TCCCGGCCAC TTTTACGCGC CAAATCCTTT
  121 TTGCCGCGAA AGAGCCACGA GCCGCCGAGC GCCGCCACAA TTGGCTGCCC CGGCCGTGAC
  181 GGGAACAACG GCGGCGGTTC CTATTGGCTT TAACGCGCCG GACCCGGCGG CGAGGGGCGG
  241 GATGGGGCGG CGGCGCGGGG CGGTACGGCC AATGGCGGGG CGGGGCGCGC GCAGGCTTTG
  301 TCCGGATGGT ACCAGGCGCG GTCTGACTCC CCGCCCCCAG CCCGGGCGTC CCCTGCTGGA
  361 GGCGTGGGGA CACGGGAACA TAGGCCCCGC CCCGCCCCGA CGCTCCCGCA TCCCACTGAC
  421 CTTTCTATAG TGCAGGTTCA GAACAGAGCC TCTCTTCTGC ACGTGACCCT CAACCTGTAG
  481 CCCCCAACAG CCTCAGCTTA GATTTCCAGA GACTGTCCCC TCTTCTTTTC AGGATCATCA
  541 GTTCCACAGC CCAGCCATCA GCCACCTCTT CCATCCTCAA CTTGCCTCAC CCATGACATT
  601 ATCCTAATCC TGCACCCAGC CCTATCCCGC TCACCCAGAG CCCAAGCTGA AGAGCGGTGC
  661 CTGGAAATGG CGAGGGGGAC GTCGGGCTTT CCTGCAAGTC CCATTTTAGC CAAAATCTCC
  721 AACAGCCAAC GTATAGCATC TGCTTGAACT CATTCATTCC CTCACTCATT CAACAAACGT
  781 TTGGAGAGCT TGCTCTGTGC CCCATCCTCA ATTCGTGGCC CTAAGGAGCT CACAGTCCAG
  841 TAGACAGGGG AACAGAAAGG TAACAGACGT TTACAATACG ATGAAGTGGC GTCTGGGCGC
  901 GGTGGCTCAC CACCGGAATC CCTGTAATCA GCTGGCGGGG TGGATCGCAT GAGCCCAGGA
  961 GTTCGAGACC AGCCCTGATC AACATGGTGA AAGACCCCAT CTCTACAAAA AATACCAAAA
 1021 AATTTAGCCA GGCATGGTTG GCGTGTGCCC GTAGTCCCAG CTACTTGGGA AGCCTGAGTG
 1081 GGAAGGATTG GCGTGGAGCT CTGAGAAGGT TCGAGCCTGG CAGTTGAGCC TTAATGTGAA
1141 TCTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles