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pGL4-phE2F1

Promoter collection, Human E2F1 promoter

Catalog number RDB07810
Resource name pGL4-phE2F1
Alternative name U3022-2
Clone info. PCR amplified human E2F1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 33687850 to 33686378(NC_000020.11); relatively -1465 to +8, where +1 corresponds to 1 nt of NM_005225.3.
Comment PCR cloning, forward primer :aaggggacatggcattgtta; reverse primer : aaagtcccggccacttttac.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human E2F transcription factor 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human E2F transcription factor 1

          Reference sequence
            

          Distribution information

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          RDB07810 pGL4-phE2F1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07810_A4Hb.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07810_A4Hba.seq check
          >07810_07810_A4Hb_pGL4-4174F_E07_13.ab1
              1 AATTGTGTCC AGAAATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
             61 GATAAGGGGA CATGGCATTG TTAAAATGCA GATTCTTGGG CACCTAGAGA TTTTAAATTG
            121 GCAGGGCCAG GGTGGGCCCA AAATTAGCAA GTGACCACGT GGTTCTGAAG CCAGTGGCCT
            181 AAGGACCACC CTTGCAGAAC CGTGGTCTCC TTGTCACAGT CTAGGCAGCC TCTGGCTTAG
            241 CCTCTGTTTC TTTCATAACC TTTCTCAGCG CCTGCTCTGG GCCAGACCAG TGTTGGGAGG
            301 AGTCGCTACT GAGCTCCTAG ATTGGCAGGG GAGGCAGATG GAGAAAAGGA GTGTGTGTGG
            361 TCAGCATTGG AGCAGAGGCA GCAGTGGGCA ATAGAGGAAG TGAGTAAATC CTTGGGAGGG
            421 CTCCCTAGAA GTGATGTGTT TTCTTTTTTT GTTTTAGAGA CAGGATCTCG CTCTGTCGCC
            481 CAGGCTGGTG TGCAGTGGCA TGATCATAGC TCACTGCAGC CTCGACTTCT CGGGCTCAAG
            541 CAATCCTCCC ACCTCAGCCT CCCAAGTAGC TGGGACTACG GGCACACGCC ACCATGCCTG
            601 GCTAATTTTT GTATTTTTTG TAGAGATGGG TCTTCACCAT GTTGATCAGG CTGGTCTCGA
            661 ACTCCTGGGC TCATGCGATC CACCCCGCCA GCTGATTACA GGGATTCCGG TGGTGAGCCA
            721 CCGCGCCCAG ACGCCACTTC ATCGTATTGT AAACGTCTGT TACCTTTCTG TTCCCCTGTC
            781 TACTGGACTG TGAGCTCCTT AGGGCCACGA ATTGAGGATG GGGCACAGAG CAAGCTCTCC
            841 AAACGTTTGT TGAATGAGTG AGGGAATGAA TGAGTTCAAG CAGATGCTAT ACGTTGGCTG
            901 TTGGAGATTT TGGCTAAAAT GGGACTTGCA GGAAAGCCCG ACGTCCCCCT CGCCATTTCC
            961 AGGCACCGCT CTTCAGCTTG GGCTCTGGGT GAGCGGGATA GGGCTGGGTG CAGGATTAGG
           1021 ATAATGTCAT GGGTGAGCAA GTTGGAGGAT GAGAGTGGCT GATGCTGGCT GTGTACTGAT
          1081 GATCTTGAAA GAGAGGACAG TCTCTGGCAA TCTTAAGCCT TGAAGGCCTT GT
          //
          Primer: pGL4-136R
          Sequence file: RDB07810_A4Hbb.seq check
          >07810_07810_A4Hb_pGL4-136R_F07_16.ab1
              1 GTCTCTTTGT TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGCG CATTGCCAAG
             61 CTTGGCCGCC GAGGCCAGAT CTTGATAAAG TCCCGGCCAC TTTTACGCGC CAAATCCTTT
            121 TTGCCGCGAA AGAGCCACGA GCCGCCGAGC GCCGCCACAA TTGGCTGCCC CGGCCGTGAC
            181 GGGAACAACG GCGGCGGTTC CTATTGGCTT TAACGCGCCG GACCCGGCGG CGAGGGGCGG
            241 GATGGGGCGG CGGCGCGGGG CGGTACGGCC AATGGCGGGG CGGGGCGCGC GCAGGCTTTG
            301 TCCGGATGGT ACCAGGCGCG GTCTGACTCC CCGCCCCCAG CCCGGGCGTC CCCTGCTGGA
            361 GGCGTGGGGA CACGGGAACA TAGGCCCCGC CCCGCCCCGA CGCTCCCGCA TCCCACTGAC
            421 CTTTCTATAG TGCAGGTTCA GAACAGAGCC TCTCTTCTGC ACGTGACCCT CAACCTGTAG
            481 CCCCCAACAG CCTCAGCTTA GATTTCCAGA GACTGTCCCC TCTTCTTTTC AGGATCATCA
            541 GTTCCACAGC CCAGCCATCA GCCACCTCTT CCATCCTCAA CTTGCCTCAC CCATGACATT
            601 ATCCTAATCC TGCACCCAGC CCTATCCCGC TCACCCAGAG CCCAAGCTGA AGAGCGGTGC
            661 CTGGAAATGG CGAGGGGGAC GTCGGGCTTT CCTGCAAGTC CCATTTTAGC CAAAATCTCC
            721 AACAGCCAAC GTATAGCATC TGCTTGAACT CATTCATTCC CTCACTCATT CAACAAACGT
            781 TTGGAGAGCT TGCTCTGTGC CCCATCCTCA ATTCGTGGCC CTAAGGAGCT CACAGTCCAG
            841 TAGACAGGGG AACAGAAAGG TAACAGACGT TTACAATACG ATGAAGTGGC GTCTGGGCGC
            901 GGTGGCTCAC CACCGGAATC CCTGTAATCA GCTGGCGGGG TGGATCGCAT GAGCCCAGGA
            961 GTTCGAGACC AGCCCTGATC AACATGGTGA AAGACCCCAT CTCTACAAAA AATACCAAAA
           1021 AATTTAGCCA GGCATGGTTG GCGTGTGCCC GTAGTCCCAG CTACTTGGGA AGCCTGAGTG
           1081 GGAAGGATTG GCGTGGAGCT CTGAGAAGGT TCGAGCCTGG CAGTTGAGCC TTAATGTGAA
          1141 TCTCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl