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pGL4-phARNT

Promoter collection, Human ARNT promoter

Catalog number RDB07731
Resource name pGL4-phARNT
Alternative name U3013-1
Clone info. PCR amplified human ARNT promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 150878102 to 150876641(NC_000001.11); relatively -1503 to -41, where +1 corresponds to 1 nt of NM_001668.4; relatively -1392 to +70, where +1 corresponds to 1 nt of XM_005245151.2.
Comment F-promoter: gctgaggcaggataactgct; R-promoter: aggggaaaagaaaggccact.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human aryl hydrocarbon receptor nuclear translocator genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human aryl hydrocarbon receptor nuclear translocator

          Reference sequence
            

          Distribution information

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          RDB07731 pGL4-phARNT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07731_A4La.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07731_A4Laa.seq check
          >07731_07731_A4La_2_pGL4-4174F_G04_19.ab1
              1 ACTGGGTTCC AGAAACATTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG
             61 AGGATGCTGA GGCAGGATAA CTGCTTGAAC CCGGGAGGGA GAGGTTGCAG TGAGCCAAGA
            121 TCGCACCACT GCACTCCAGC CTGGGCGATG GAGCAAGACT CCGTCTCAGA AAAAAATAAA
            181 GAAAAAAGAA AATAAATCTG CACAGCCATC ATCTTATTGA TTCCCTATTA AAAACTCCTC
            241 ACTGGCCAGG TGTGGTGGCT CACGCCTGTA ATCCCAACGG TTTGTGAGAC CGAGGCGGGC
            301 AGATTGCCTG ATGTCAGGAG TTCGAGACCA GCCTGGCCAA CGTCGTGAAA CTCCATCTCT
            361 ACTAAAAATA CAAAAAACAT TAGCCAGGCG TGTGGCGTGC GCCTGTAATC CCAGCTACTC
            421 AGGAGACTGA GGCAGGGGAA TTGCTTGAAC CGGGGAGGTG GAGGTTGCAG TGAGCCGAGA
            481 TTTGCGCCAC TGCACTCCAG CCTGGGGCGA CAGAGAGAGA CTCTGTCTCA AAAACAGACA
            541 AACAAAACTC CTCACTCTGT CCCTGTCACT GACCAAAGAA GTTACCCATT CTCAAATTAC
            601 TTTCTTCATC CATTGCCTCC AATTTTGGCT GGCAATATCT CCCATACCAA ACCGTTAACA
            661 CCTGGATCCT TTTAGACTAC CGGGTTCCAT AGCAGGCATT CAAAAGGTAG TCATTTAACT
            721 TTTTTCACCA GGACAAAAAG TCCTTTAAAA AAGGAAAAAA AACTGGAAAG GGATTACAAT
            781 CACCCATGGT GAAGTTTAGG GTGCTTCTTT AGCACCTAAA GAGGATTTAC CAAGAATACA
            841 TGTGTCCTGC ACCGCGAACC AATTAGACAG AAATCTACCA CAAGCGGAAG GGTTTTAGAA
            901 AATAATCCCT CTTTTTTTTG AGACGGAATT CGCTCTTTGT GTTGCCCAGA ATGGGGGGTG
          961 AGATGGGCGG CCATATCTCG GT
          //
          Primer: pGL4-136R
          Sequence file: RDB07731_A4Lab.seq check
          >07731_07731_A4La_2_pGL4-136R_H04_22.ab1
              1 CCCTTATGTT TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT
             61 TGGCCGCCGA GGCCAGATCT TGATAGGGGA AAAGAAAGGC CACTCCCCAG AAGCGGCGCC
            121 TGACCGCCGC CTCCGCTACC GCGGAATCCA AGATGGCGGC TTCAGCAGCC CCCAGCATCC
            181 CAGCCCCTGC GGCGGGGAGA CCCCGCCTAT ATAGTAGGCG GAGTCAACAC ACGGCCAGCC
            241 TCCTAAGAAA AATTGACCAA TGAAAATGCC AAATCGTCCA AGGGCAGGGC TACTCACTGC
            301 GCCAGCCAGT CCAGTGAAGG GAGGGCGAGG AAGAAGTCCA GCTGTCTCCC TAATCTCGCC
            361 CTCCTTGCGT AAGAGGTCAA TGACCGAGTA ACGTCATTGA AATGCAATTA AAATGGGTAT
            421 TTTAGGCTGG GCGCGGTGGC TCGCGTCTGT AATCCCAGCA CTTTGGGAGG CCGAGGCGGC
            481 CGGATCACCT GTGGCCAGGG GTTCGAAACC AGCCTGGCCA ACATGGCGAA ACCCCGTCTC
            541 TACTAAAAAT ACAAAATTAG CCGGGCGTGG TGGCGCATGC CTGTAATCCC AGCTTCTTGG
            601 GAGGCTGAGG CAGGAGAATC GCTTGAACCT GGGAGGCAGA GGTTGCAGTG AGCCGAGATA
            661 GCGCCACTGC ACGCCAGTCT GGGCAACAAG AGCGAAACTC CGTCTCAAAA AAAAAAAAGG
            721 ATATTTTACT AAAAACCTTC GCTTGGTGGT GGATTCGTCT GAGTGGTTGC CGTGCAGACA
            781 ATGTATCTTG TAAATCCTCT TTAGGTGCTA AAGAGCACCT AAACTTCACA TGGTGATTTG
            841 TAATCCTTTC CAGTTTTTTT CCTCTTTTAA GGACTTTTGT CCCTGGTGAA AAAGTTAAAT
            901 GACTAACTTT TGAATGCCTG CTATGGAACC GGTAGTCTAA AAGGATCCAG GTGTTAACGG
            961 TTTGGTATGG GAGATATTGC CAGCCAAAAT TGGAGGCAAT GGATGAAGAA AGTAATTTGA
           1021 GAATGGGTAA CTTCTTTGGG TCAGTGACAG GGACAGAGTG AGAGTTTTGT TGGTCTGTTT
           1081 TTGAGAACAG AGTCTCTCTC TGTCGGCCCA GGCCTGATGC AGTGGCGGCA TCTCGGCTTC
          1141 ACTGGCAAGC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl