RDB07731 - pGL4-phARNT

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pGL4-phARNT (#RDB07731)

Promoter collection, Human ARNT promoter

Alternative name	U3013-1
Clone info.	PCR amplified human ARNT promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 150878102 to 150876641(NC_000001.11); relatively -1503 to -41, where +1 corresponds to 1 nt of NM_001668.4; relatively -1392 to +70, where +1 corresponds to 1 nt of XM_005245151.2.
Comment	F-promoter: gctgaggcaggataactgct; R-promoter: aggggaaaagaaaggccact.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human aryl hydrocarbon receptor nuclear translocator genomic DNA
Depositor\|Developer	DNA Bank, \|

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07731	pGL4-phARNT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phARNT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07731).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07731_A4La.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07731_A4Laa.seq
>07731_07731_A4La_2_pGL4-4174F_G04_19.ab1 1 ACTGGGTTCC AGAAACATTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG 61 AGGATGCTGA GGCAGGATAA CTGCTTGAAC CCGGGAGGGA GAGGTTGCAG TGAGCCAAGA 121 TCGCACCACT GCACTCCAGC CTGGGCGATG GAGCAAGACT CCGTCTCAGA AAAAAATAAA 181 GAAAAAAGAA AATAAATCTG CACAGCCATC ATCTTATTGA TTCCCTATTA AAAACTCCTC 241 ACTGGCCAGG TGTGGTGGCT CACGCCTGTA ATCCCAACGG TTTGTGAGAC CGAGGCGGGC 301 AGATTGCCTG ATGTCAGGAG TTCGAGACCA GCCTGGCCAA CGTCGTGAAA CTCCATCTCT 361 ACTAAAAATA CAAAAAACAT TAGCCAGGCG TGTGGCGTGC GCCTGTAATC CCAGCTACTC 421 AGGAGACTGA GGCAGGGGAA TTGCTTGAAC CGGGGAGGTG GAGGTTGCAG TGAGCCGAGA 481 TTTGCGCCAC TGCACTCCAG CCTGGGGCGA CAGAGAGAGA CTCTGTCTCA AAAACAGACA 541 AACAAAACTC CTCACTCTGT CCCTGTCACT GACCAAAGAA GTTACCCATT CTCAAATTAC 601 TTTCTTCATC CATTGCCTCC AATTTTGGCT GGCAATATCT CCCATACCAA ACCGTTAACA 661 CCTGGATCCT TTTAGACTAC CGGGTTCCAT AGCAGGCATT CAAAAGGTAG TCATTTAACT 721 TTTTTCACCA GGACAAAAAG TCCTTTAAAA AAGGAAAAAA AACTGGAAAG GGATTACAAT 781 CACCCATGGT GAAGTTTAGG GTGCTTCTTT AGCACCTAAA GAGGATTTAC CAAGAATACA 841 TGTGTCCTGC ACCGCGAACC AATTAGACAG AAATCTACCA CAAGCGGAAG GGTTTTAGAA 901 AATAATCCCT CTTTTTTTTG AGACGGAATT CGCTCTTTGT GTTGCCCAGA ATGGGGGGTG 961 AGATGGGCGG CCATATCTCG GT //
Primer: pGL4-136R Sequence file: RDB07731_A4Lab.seq
>07731_07731_A4La_2_pGL4-136R_H04_22.ab1 1 CCCTTATGTT TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATAGGGGA AAAGAAAGGC CACTCCCCAG AAGCGGCGCC 121 TGACCGCCGC CTCCGCTACC GCGGAATCCA AGATGGCGGC TTCAGCAGCC CCCAGCATCC 181 CAGCCCCTGC GGCGGGGAGA CCCCGCCTAT ATAGTAGGCG GAGTCAACAC ACGGCCAGCC 241 TCCTAAGAAA AATTGACCAA TGAAAATGCC AAATCGTCCA AGGGCAGGGC TACTCACTGC 301 GCCAGCCAGT CCAGTGAAGG GAGGGCGAGG AAGAAGTCCA GCTGTCTCCC TAATCTCGCC 361 CTCCTTGCGT AAGAGGTCAA TGACCGAGTA ACGTCATTGA AATGCAATTA AAATGGGTAT 421 TTTAGGCTGG GCGCGGTGGC TCGCGTCTGT AATCCCAGCA CTTTGGGAGG CCGAGGCGGC 481 CGGATCACCT GTGGCCAGGG GTTCGAAACC AGCCTGGCCA ACATGGCGAA ACCCCGTCTC 541 TACTAAAAAT ACAAAATTAG CCGGGCGTGG TGGCGCATGC CTGTAATCCC AGCTTCTTGG 601 GAGGCTGAGG CAGGAGAATC GCTTGAACCT GGGAGGCAGA GGTTGCAGTG AGCCGAGATA 661 GCGCCACTGC ACGCCAGTCT GGGCAACAAG AGCGAAACTC CGTCTCAAAA AAAAAAAAGG 721 ATATTTTACT AAAAACCTTC GCTTGGTGGT GGATTCGTCT GAGTGGTTGC CGTGCAGACA 781 ATGTATCTTG TAAATCCTCT TTAGGTGCTA AAGAGCACCT AAACTTCACA TGGTGATTTG 841 TAATCCTTTC CAGTTTTTTT CCTCTTTTAA GGACTTTTGT CCCTGGTGAA AAAGTTAAAT 901 GACTAACTTT TGAATGCCTG CTATGGAACC GGTAGTCTAA AAGGATCCAG GTGTTAACGG 961 TTTGGTATGG GAGATATTGC CAGCCAAAAT TGGAGGCAAT GGATGAAGAA AGTAATTTGA 1021 GAATGGGTAA CTTCTTTGGG TCAGTGACAG GGACAGAGTG AGAGTTTTGT TGGTCTGTTT 1081 TTGAGAACAG AGTCTCTCTC TGTCGGCCCA GGCCTGATGC AGTGGCGGCA TCTCGGCTTC 1141 ACTGGCAAGC T //